Green block organelle presentations Cell Membrane by Max Guiffre and Bo Picking Function PLASMA MEMBRANE: -Protects the cell from its surroundings. -Monitors the substances that go in and out of the cell. INTERNAL MEMBRANE: -Protects the organelle from its surroundings. -Monitors the substances that go in and out of the organelle. Structure -Made up of phospholipids and proteins scattered throughout. -Porous structure that allows certain substances to come in and out. Location PLASMA MEMBRANE: -In BOTH Eukaryotic and Prokaryotic cells. -It is located on the outer edge of each cell but inside of the cell wall. -In plant, animal, and bacteria cells. INTERNAL MEMBRANE: -Found in plant and animal cells. -Not found in bacteria because it is a prokaryote and prokaryotes don’t have organelles that have internal cell membranes. Analogy A plasma cell membrane is like the walls surrounding a building giving it protection, and the doors act like the proteins, allowing things to go in and out. Then, inside the building there are other doors, like a freezer door in a grocery store, protecting specific things like the internal membrane would do. PLANT CELL WALL Structure: Primary Wall is relatively thin and flexible to allow for mobility and growth: Consists of fibers of cellulose embedded in a matrix of other polysaccharides and proteins Outermost layer contains cell binding polysaccharides: Between adjacent cells is a layer of sticky polysaccharides, called pectins plasmodesmata connect adjacent plant cells cytosol -- the part of the cytoplasm that is not held by any of the organelles in the cell -- passes through the plasmodesmata allowing small molecule nutrients to pass through Function: To keep a rigid structure for the plant To protect the internal systems of the cell Keeps cells in a unified and organized body Absorbs nutrients Analogy: Cell wall acts like a sand sifter BACTERIA CAPSUL Structure: Sticky layer of polysaccharides or proteins Function: Enables Prokaryotes to adhere to a surface or to other individuals in a colony Shields pathogenic Prokaryotes from attacks by their host’s immune system Analogy: Acts like a suite of armor over the bacteria Cytoplasm ● Provides a storage place for organelles, enzymes, and other cell parts. Gives cells their shape and allows easy movement of material between the organelles that help the cell to function. ● Cytoplasm is a clear, thick, watery substance that fills all cells. It is composed of water, salt, and proteins and is enclosed by the plasma membrane. ● In prokaryotes, the cytoplasm contains all the contents of the cell. New research shows rod-shaped bacteria and Archaea contain a cytoskeleton. ● In eukaryotes, cytoplasm is organized by a protein framework called cytoskeleton, in which the cell organelles move by cytoplasmic streaming. ● Cytoplasm is in plant, animal, and bacterial cells and is abundant in all types of these cells. Cytoplasm ● Cytoplasm is like a fruit jello cup. The fruit pieces are the organelles Animal Cell and the jello is the cytoplasm which is working to hold together the fruit. Plant Cell Bacterial Cell DNA-Prokaryote ●DNA stores the genetic information of a cell. DNA is transcribed into messenger RNA that is translated into proteins. ●Prokaryotes typically have a single, large looping strand of chromosomal DNA contained in the nucleoid. ●Many prokaryotes also contain smaller circular DNA-Prokaryote • DNA of a (DNA) prokaryote is like an entrepreneur. He has an idea that he has formed Bacterial Cell The Nucleus Jamie Mazzola & Aidan Braun Structure Nuclear Envelope ● ● ● ● A double membrane that encloses the nucleus Helps control the flow of materials in and out of the nucleus Each membrane is a separate phospholipid bilayer Protein lined pores control large molecule flow, connect to endoplasmic reticulum Nucleolus ● Synthesizes ribosomal RNA (rRNA) ○ rRNA used in the synthesis of proteins ○ Proteins from cytoplasm + rRNA = Ribosome subunits Chromatin ● ● Forms Chromosomes Complex of Proteins and DNA surrounding the Nucleolus ○ DNA wrapped around 8 proteins called histones = Nucleosomes ○ Multiple nucleosomes in string like formation = Chromatin Function and Location xxxxxxxxxxxxxxxxxxxxxxxxx xx ●Located in eukaryotic cells ○Plants and animals (not bacteria) ●Towards the middle of a cell ●Multinucleate cells x x Analogy The Nucleus is like the operations room (command center) of a battleship. Comman d center Endoplasmic Reticulum By Josie Kampe and Nathaniel Lui Functio ns: SMOOTH ER (lacks ribosomes) : 1. Produce lipids (oils, steroids and phospholipids) 2. Detoxifies potentially harmful substances (drugs and alcohol) -breaks it down into water soluble substances 3. Storage of calcium ions (releases calcium ions into cytosol when the brain sends a nerve signal to contract muscle) ROUGH ER (studded with ribosomes): 1. Produces proteins- ribosomes receive a specific sequence from the mRNA, recruits and folds amino acids (secondary and tertiary structure) and attaches to sugar chains creating glycoproteins , then part of the membrane from the Rough ER separates along with the glycoprotein. 2. (In order to sustain its own loss^) Produces its own membrane wall through phospholipids and membrane proteins Structure: • Ribosomes are bound to the surface of ER by membrane tissue (only in Rough ER) • Made of membrane-enclosed flattened sacs and tubes called cisternae--membranes are made of lipids • Vesicles to transport substances made • Big surface area • Structure in relation to function: big surface area allows for quicker detoxification and lipid and protein synthesis Location: • Inside Eukaryote cell (most plant and animal cells) • Within the Cytoplasm • Rough ER is located throughout the cell but mostly stationed around the nucleus and next to the Golgi Apparatus • Smooth ER is located on the outside of the Rough ER • Due to its function, the RER is most abundant in liver and pancreas cells, or cells of any organ which produces a lot of proteins, and the SER is most abundant in cells that release oils The ER is the factory of the cell because it produces and stores many substances used throughout the body and transports them to other cell organelles (such as the Golgi Apparatus). Golgi Apparatus Function & Structure • Modifies and sorts macromolecules as well as well as formation of lysosomes and transport vesicles. • A Golgi Apparatus is a stack of cup-shaped, membrane-covered, deflated balloon-like sacs • It looks like a stack of deflated balloons (see title slide picture). Location & Analogy • It is found in Eukaryotes (plant and animal cells, not bacterial ones). • It is usually found near the cell’s nucleus and adjacent to the endoplasmic reticulum output points (#6 in the image below). • In both plant and animal cells there is only one Golgi Apparatus. It can vary in amount of layers depending on the type of cell it is in. • It is like a processing station which sorts and makes edits to or refines the raw products Lysosomes Katie Williamson and Erik Prakken What are Lysosomes? • Round, membrane enclosed sacs of digestive enzymes –The enzymes contained within lysosomes are known collectively as acid hydrolases and work best in acidic environments • Located in the cytoplasm in the cell • Found in Eukaryotes (Animals and Plants) –All Eukaryotic cells have lysosomes –Each cell has about 50-1000 lysosomes Function of Lysosomes • Used in digestion • Provides acidic environment for its enzymes while isolating them from the rest of the cell, fuse with vacuoles (which contain food particles) and digest the food. • Serve as recycling centers – ex) white blood cells engulf bacteria and destroy them using lysosomes. • Analogy: Lysosomes would be waste management or recycling committee in a factory setting Central Plant Vacuole Structure: Central Vacuole ● Large blob contained by membrane. ● Can be filled with many different things: water, food, pigment, waste products, poisons, etc. (mostly water) Function of the Vacuoles Vacuoles are the backpack of the Cell- capable of holding large quantities of many different substances--water, food, poison to name a few. Vacuoles collect and expel excess water from within the cell. Cytoskeleton and Centrioles by Charlotte Clapham Cytoskeleton- the Movers and Shapers of a cell Cytoskeleton is found in Eukaryotic cells (in animal and plant cells) Cytoskeleton is a network of fibers that is found throughout the cell, linking organelles to other parts of the cell, and helping make up the interior structure of the cell. Strands are very concentrated near the membrane Fibroblast and cancer cells tend to have an abundance of Cytoskeleton. fibroblast cells move around a lot, and the cytoskeleton can aid with cell movement. Function: Cell shape- the cytoskeleton helps to determine the structure of a cell (like its skeleton) Movement inside cell- materials get around inside the cell by “riding” along the cytoskeleton strands Movement of cell Cell division (through the centrioles) Analogie: The skeleton of a building (support system, elevator shafts, stairs) Filaments of the Cytoskeleton The cytoskeleton is made up of 3 parts, called filaments: - Microtubules - Thickest of the filaments- hollow tube - Materials and organelles move throughout cell by riding on the microtubules - - Help make up cilia and flagella - - use protein motors- this means that ATP energy powers their movement extensions of the cell which help liquid move past the cell, and help the cell move Help make up centrioles, and aid in cell division (more detail on next slide) Intermediate Filaments - Second thickest of the filaments - Reinforce the shape of the cell, and help keep organelles in place (like the nucleus) - Permanent fixtures within the cell Microfilaments (Actin filaments) Centrioles Location: In most animal cells (Eukaryotic) Plant and bacteria cells do not have centrioles- plant cells can reproduce without them Made up of 9 groups of microtubules that make a ring-structure. These structures are very small within the cell Found in pairs, usually at a right angle to each other, near the nucleus (before cell division). In animal cells, the centrioles are found within the centrosome (an organelle) There may be more of an abundance of these in lung cells, due to their abundance of cilia Function: Organizes cellular division, help to determine position of nucleus in newly formed cells, make cilia During cell division: Nucleus membrane is breaking down → proteins in microtubules grab onto chromosomes in the nucleus → This helps to pull apart the chromosomes, divide the cell, and give each new cell a set of chromosomes. Before division, there will be one pair of Centrioles, but during division, another pair will be created, and each pair goes to a separate side of the cell. Analogie: Dividing a large bowl of spaghetti into smaller bowls with tongs The Chloroplast By: Leon Xiong What is a chloroplast? Chloroplasts are the main source of energy for a plant. They do this by perforing photosynthesis which uses sunlight in order to combine CO2 (Carbon Dioxide) and H20 (Water) to create C6H12O6 (Glucose). This is all dependent on the tiny green chlorophyll molecules within a chloroplast. The Outer/Inner Membrane The outer membrane is a thin membrane which surrounds the organelle which is permeable by small organic molecules. The inner membrane is a thicker layer surrounding the inside of a the organelle. This layer is not permeable and is studded with transport proteins. A transport protein is a protein which is incharge Inner Structure Stroma and Grana stacks are stacks of Thykaloids. Thykaloids are the site where the light based reaction that combines H20 and C02 happens. Chloroplasts are held together by tubes called stroma lamellae. These tubes keep the thykaloids a safe distance from each other. Making Energy When sunlight hits a chloroplast, it converts from light energy into energy compounds like ATP and NADPH. Afterwards, the energy rich compounds move into the stroma where Molecular reactions catalyzed by various enzymes happen. The end products of Glucose, CO2 and Water are formed. Analogy A chloroplast is similar to a wind turbine. A wind turbine takes energy from the wind and transforms it into electricity. Similarly the Chloroplast takes the ergy from the sun and transforms it into Glucose. MITOCHONDRIA by Varsha Iyer and Emily Zhang Location/Function ● Eukaryotes have mitochondria floating freely throughout the cell • Found in plant and animal cells • Mitochondria is essential to tissues and organs that need a lot of energy • • • ● Fat: stores a lot of energy Muscle: responds quickly to do work Liver: has over 200 different jobs, meaning it has lots to do Carries out cellular respiration in nearly all eukaryotic cells, converting the chemical energy of foods to the chemical energy of the molecule, ATP ○ ○ Cellular respiration: the reaction that occurs in the mitochondria when oxygen and glucose are carried to your cells to produce ATP, water, and carbon dioxide. ATP (Adenosine triphosphate): the main energy source for cells. ATP releases energy when its phosphate bonds are hydrolyzed. ■ Yes, plants get their energy through photosynthesis, but also by breaking down macromolecules into monosaccharides ■ Example: A cloudy day without enough sun Structure • A mitochondrion is enclosed by the outer membrane and the inner membrane – Outer membrane: acts as the “skin” of a cell and covers it – Inner membrane: highly folded and contains many embedded protein molecules that function in ATP synthesis. • The folds, or cristae, increase the membrane’s surface area, helping the mitochondria produce ATP. • Membranes are made of phospholipid bilayers with a unique collection of embedded proteins. • The inner and outer membranes have two internal compartments – 1) intermembrane space: a narrow region between the inner and outer membranes. – 2) The mitochondrial matrix: enclosed by the inner membrane, which contains mitochondrial DNA and ribosomes (transfers RNA to synthesize proteins), as well as enzymes that catalyze some of the reactions of cellular respiration. Analogy - A Car • Mitochondria is the “powerhouse” of a cell –similar to the engine of a car. • The main function of mitochondria is converting chemical energy of foods to the chemical energy of the molecule. –the main function of an engine is to convert gasoline into energy that powers the car. Car body similar to outer membrane Engine outer body similar to inner membrane References - Textbook pg 63: Reece, Jane B., and Neil A. Campbell. Biology: Concepts & Connections. 8th ed. Boston: Pearson, 2015. Print. - "Molecules of Life." BBC. N.p., 2014. Web. 25 Oct. 2015. <http://www.bbc.co.uk/schools/gcsebitesize/sc ience/add_ocr_gateway/living_growing/molec ulesrev1.shtml>. - Brain, Marshall. "How Car Engines Work." HowStuffWorks. N.p., n.d. Web. 25 Oct. 2015. Ribosomes By: Katie Stevenson and Karina Kropp Structure Made up of… ~ Small subunit → reads RNA ~ Large subunit → joins amino acids to form polypeptide bonds ~ Found in both prokaryotes and eukaryotes. ~ The two units come together to create the one larger ribosome organelle. Location ~ In the cell free ribosomes (produces proteins for the cell) are suspended in the cytoplasm and bound ribosomes (produces proteins transported out of the cell) are attached to the outside of the endoplasmic reticulum or nuclear envelope. ~ Found in both prokaryotes and eukaryotes. ~ Ribosomes are found in plants, animals, and bacterial cells. ~Ribosomes are abundant in parenchyma (plants) and epithelial cells (animals)