Name: Date: Biology 11: Kingdom Cnidaria Lab Purpose: -To examine members of the Phylum Cnidaria and be able to: a) Examine and identify the phyla’s unifying characteristics. b) Describe how the phyla carries out its life functions. d) Compare and contrast the structure, general function and mobility of Cnidarian polyp and medusa body plans. e) Describe the advantages of a mobile stage in the life cycle of Cnidarians. Observing Living Cnidarians (Sections I and II) 1. Obtain a living Cnidarian from the stock containers and place it in a small dish. 2. Observe the Cnidarian under a dissecting microscope and illuminate it using the overhead light only. If you use the bottom light you will kill your Cnidarian as the water will become too hot. Reminder: All drawings must be in pencil and accompanied by magnifications! Section I: Hydra Description: Hydra are small aquatic predators that feed on small zooplankton. They use their tentacles and cnidocytes to capture prey. Hydra lack a medusa stage in their life cycle and only exist as polyps reproducing sexually with gametes or asexually by budding. Hydra are fairly sessile, attaching to an aquatic substrate and waving their tentacles waiting for prey. They are, however, capable of locomotion using a somersault or inchworm approach. Biology 11: Phylum Cnidaria Page 1 a) Brown Hydra Observation 1. Follow Observing Living Cnidarians instructions 2. Your hydra may contract into a small ball for several minutes if disturbed. You may need to be patient and wait for it to relax again. 3. Carefully draw the hydra in the circle below. Label: epidermis, gastrodermis, gastrovascular cavity, mesoglea, tentacles, mouth, body column and basal disc. Notes: _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ 4. Observe your Hydra contracting and extending its tentacles. Do they all extend and contract at the same time? Why do you think this is? _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Biology 11: Phylum Cnidaria Page 2 5. Describe how your Hydra moves (what type of skeleton does it use to move?). Would you say that it is sessile, sedentary or motile? ____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ b) Feeding the Hydra 1. Obtain a sample of living daphnia (water fleas) and place them in the small dish containing your hydra. Observe the hydra capture and eat the daphnia. Describe this process using appropriate scientific vocabulary below: _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 3. In your own words, define the term cnidocyte and list its functions. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Biology 11: Phylum Cnidaria Page 3 2. Observe a prepared slide of a hydra tentacle. Draw what you see and label the cnidocytes (stinging cells). Notes: _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ 3. Where do you expect to find the greatest density of cnidocytes? Why does this distribution make sense? _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Biology 11: Phylum Cnidaria Page 4 c) Budding Hydra 1. Follow Observing Living Cnidarians instructions. 2. Carefully draw what you observe and label an asexual bud. Take special note of the two tissue layers and the cellular differentiation Notes: _______________________________________ _______________________________________ _______________________________________ _______________________________________ Section II: Obelia Description: Obelia are polymorphic. Obelia polyps form a sessile colony. There are two types of polyps in the colony; a feeding polyp equipped with tentacles surrounding the mouth and a reproductive polyp which produces asexual medusae by budding. These medusae are free swimming and represent the sexual stage of the life cycle. At maturity, gonads on each meduase release gametes (sperm or egg) into the water. After fertilization, the larva locates a suitable location and attaches to the substrate and develops into a new polyp colony. Biology 11: Phylum Cnidaria Page 5 1. Examine a prepared Obelia slide under a light microscope. Draw what you observe below and label: Polyp, tentacles, mouth and gastrovascular cavity. Notes: _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ 2. How are Obelia similar to Hydra? How are they different? _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 3. Obelia follow an Alternation of Generations life cycle, with both a sexual and asexual generation. Which of these generations does the Obelia colony represent? Why are the benefits of this type of reproduction? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ Biology 11: Phylum Cnidaria Page 6 Section III: Aurelia Description: Marine jellies (or jellyfish) are often encountered along beaches or bay systems. The large, free-swimming medusa move by pulsing actions of their bell. Many species have powerful nematocysts used to capture prey and can deliver an uncomfortable sting to potential predators as well. The tentacles are positioned along the margin of the bell-shaped medusa. Oral arms surround the mouth and hang under the bell. Four circular gonads can be seen in mature specimens during the breeding season. 1. Examine the preserved Jelly sample (or an illustration in one of the books around the lab) and describe its symmetry and body form. _____________________________________________________________________________________ _____________________________________________________________________________________ 2. Where are the tentacles located? ________________________________________________________ 3. Locate the gonads in the centre of the specimen. a) In your own words explain what gonads are and in what stage in the Aurelia lifecycle you would find them. _____________________________________________________________________________________ Biology 11: Phylum Cnidaria Page 7 ____________________________________________________________________________________ _____________________________________________________________________________________ b) How would you describe the shape of the gonads? How many are there? _____________________________________________________________________________________ _____________________________________________________________________________________ 4. Define the term mesoglea in your own words and describe where you would find it in the specimen. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 5. Conduct your own research to discover the ecological role of Jellies. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Biology 11: Phylum Cnidaria Page 8