Supporting material
Figures
Fig. S1. BT hybrids (brown trout mother) suffered significantly higher mortality during the initial
100 days of juvenile rearing than did other groups (p < 0.001), including (left to right) brown
trout, AS hybrids (salmon mother), and Atlantic salmon. Error bars represent one standard
deviation
1
Tables
Table S1. Parental sources of gametes used in experimental crosses.
Source
Family
1
2
3
4
5
6
Cross
Trout
Trout
Trout
Trout
Trout
Trout
Paternal
Trout 7
Trout 8
Trout 9
Trout 1
Trout12
Trout10
Maternal
Trout13
Trout14
Trout15
Trout 16
Trout 17
Trout 18
7
8
AS Hybrid
AS Hybrid
Trout 4
Trout 12
9
10
11
12
BT Hybrid
BT Hybrid
BT Hybrid
BT Hybrid
Salmon 1 (transgenic)
Salmon 2 (transgenic)
Salmon 3 (transgenic)
Salmon 4 (transgenic)
Trout 19
Trout 20
Trout 21
Trout 22
13
14
15
16
17
18
19
Salmon
Salmon
Salmon
Salmon
Salmon
Salmon
Salmon
Wild salmon 6
Wild salmon 9
Salmon 5 (transgenic)
Salmon 6 (transgenic)
Wild salmon 3
Wild salmon 8
Wild salmon 2
Salmon 8 (transgenic)
Salmon 9 (transgenic)
Wild salmon 11
Wild salmon 10
Salmon 7 (transgenic)
Salmon 10 (transgenic)
Salmon 11 (transgenic)
Salmon 7 (transgenic)
Salmon 8 (transgenic)
2
1
Table S2. Mass (g) and length (mm) of groups at the start and end of 100 days rearing in common laboratory conditions. Note
2
differences in initial size among groups.
Cross
Trout (n=6)
AS hybrids (n=2)
BT hybrids (n=4)
Salmon (n=7)
Initial length (mm)
Wild-type Transgenic
22.38
NA
25.24
25.00
21.33
21.26
24.47
24.08
Initial mass (g)
Wild-type Transgenic
0.091
NA
0.139
0.137
0.087
0.082
0.144
0.138
Final length (mm)
Wild-type Transgenic
43.134
NA
46.290
55.159
39.799
51.671
43.675
51.991
Final mass (g)
Wild-type Transgenic
0.976
NA
1.296
1.977
0.810
1.536
1.040
1.535
3
4
Table S3. Comparisons of size-standardized growth rate in length (mm) and mass (g) during 100 days rearing in common laboratory
5
conditions compared to growth rate not considering size-effects.
-1
Cross
Trout (n=6)
AS hybrids (n=2)
BT hybrids (n=4)
Salmon (n=7)
Growth (mm day )
Wild-type
Transgenic
0.00659
NA
0.00572
0.00746
0.00584
0.00834
0.00565
0.00752
-1
Growth (g day )
Wild-type
Transgenic
0.0240
NA
0.0210
0.0252
0.0209
0.0277
0.0193
0.0236
Standardized Growth (%
change mm-1)
Wild-type
Transgenic
1.92
NA
1.71
2.29
1.66
2.48
1.67
2.28
Standardized Growth (%
change g-1)
Wild-type
Transgenic
1.68
NA
1.64
2.11
1.41
2.07
1.46
1.89
6
3
Table S4. Initial and final mass (g) and length (mm) and of wild-type and transgenic Atlantic salmon reared in allopatry or sympatry
with transgenic AS and BT hybrids.
Treatment
Initial mass (g)
Allopatry- Control (n=92)
Sympatry- AS hybrids (n=61)
Sympatry- BT hybrids (n=45)
Initial length (mm)
Final mass (g)
Final length (mm)
Wild-type
Transgenic
Wild-type
Transgenic
Wild-type
Transgenic
Wild-type
Transgenic
1.081
1.045
0.9569
1.484
1.572
1.644
43.86
43.93
42.36
51.55
52.38
53.05
1.454
1.246
1.136
1.766
1.606
1.693
48.23
46.13
44.52
54.43
54.09
54.42
Table S5. Growth rate (size-standardized) and condition factor (103 x Weight/Length3) of wild-type and transgenic Atlantic salmon
reared in allopatry or sympatry with transgenic AS and BT hybrids.
Treatment
Allopatry- Control (n=92)
Sympatry- AS hybrids (n=61)
Sympatry- BT hybrids (n=45)
Standardized growth
(% mm day-1)
Wild-type Transgenic
0.00973
0.00579
0.00491
0.00340
0.00485
0.00273
Standardized growth
Δ Condition factor
-1
(% g day )
(Final CF-Initial CF)
Wild-type Transgenic Wild-type Transgenic
0.0100
0.0063
1.72E-05
9.37E-06
0.0056
0.0006
2.50E-05 -8.53E-05
0.0051
0.0011
1.86E-05 -4.65E-05
4