Levin Lab Benthic Studies (1) Assess benthic faunal colonization on banks (2) Assess trophic status of restored salt marshes Goal: Develop metrics for healthy salt marsh and subtidal habitat Bank Macrofauna What are macrofauna? • small worms, clams, crustaceans and other invertebrates Significance of macrofauna: • major agents of sediment stability/instability • primary food items for bottom feeding fish, shrimp Strategy: • sample macrofauna at time intervals following bank placement Sampling and analysis methods • field - sediment coring, vertical sectioning, preservation • laboratory - sieving, sorting under dissecting microscope, • counting, species identification (~ 1 da/sample) • statistical measures of diversity and community structure What we learn: • proxy for ecosystem successional state and health Salt Marsh Trophic Function What is Trophic Function? QuickTime™ and a Photo - JPEG decompressor are needed to see this picture. A key ecosystem property, Food web and energy transfer Goal: Develop a method for assessing recovery of trophic function in restored Venice Lagoon salt marshes Rationale: Most measures of recovery examine structural properties rather than function Approach: Use stable isotopic analyses to examine food web organization (isotopic signatures differ for different plants/algae and are passed from food to consumer) Compare food webs in natural and restored salt marshes of different ages Methods: Sample primary producers and consumers, dry and powder Analyze isotopic signatures (d13C, d15N) on a mass spectrometer. Use mixing models to establish trophic structure What we learn What forms the base of the food chain in undisturbed marshes? How many trophic levels are present in a healthy marsh? Are these similar in restored marshes? After how many years? Can we develop a functional measure of marsh recovery? Salicornia? QuickTime™ and a Photo - JPEG decompressor are needed to see this picture. Spartina? Limonium? QuickTime™ and a Photo - JPEG decompressor are needed to see this picture. QuickTime™ and a Photo - JPEG decompressor are needed to see this picture. Phytoplankton? Cyanobacteria? QuickTime™ and a Photo - JPEG decompressor are needed to see this picture.