ECEU692 Subsurface Imaging Course Notes Part 2: Imaging with Light (1) Profs. Brooks and DiMarzio Northeastern University Spring 2004 January 2004 Chuck DiMarzio, Northeastern University 10471-2-1 Lecture Overview • • • • • Optical Spectrum and Sources Interaction of Light with Matter A Bit of Geometric Optics A Bit of Physical Optics Microscopes (1) – Point-Spread Function – Optical Transfer Function • Detectors and Cameras January 2004 Chuck DiMarzio, Northeastern University 10471-2-2 Classical Maxwellian EM Waves Thanks to Prof. S. W.McKnight v=c λ E H x E z H E H λ=c/υ y c=3x108 m/s (free space) υ = frequency (Hz) January 2004 Chuck DiMarzio, Northeastern University 10471-2-3 Electromagnetic Spectrum (by λ) UV= Near-UV: 0.3-.4 μ VIS= IR= 0.40-0.75μ Near: 0.75-2.5μ Vacuum-UV: 100-300 nm Mid: 2.5-30μ Extreme-UV: 1-100 nm Far: 30-1000μ 0.1 μ 10 nm =100Å 0.1 Å 1Å γ-Ray 1μ (300 THz) 10 Å X-Ray Soft X-Ray January 2004 1 mm Mm-waves Chuck DiMarzio, Northeastern University Thanks to Prof. S. W.McKnight 10 μ 100 μ = 0.1mm 1 cm 0.1 m Microwaves 10471-2-4 RF Typical Outdoor Radiance Levels Ultraviolet Visible Near IR 6000K Sun 6.9 G Lux Sunlit Cloud 6.9 k Lux Blue Sky Mid IR Far IR 300K night sky Atmospheric Passbands January 2004 Chuck DiMarzio, Northeastern University 10471-2-5 D Ml/Delta T Thermal Imaging T = 300 K 1 0.5 0 1 0 1 2 10 T = 500 K 10 10 D Ml/Delta T 0 -1 10 6 4 2 0 -1 10 January 2004 10 10 l, Wavelength, m m Chuck DiMarzio, Northeastern University 10 10471-2-6 2 Light in a Turbid Medium Input Specular Reflection Diffuse Reflection Scattering Absorption Direct Transmission Diffuse Transmission January 2004 Chuck DiMarzio, Northeastern University 10471-2-7 Example Interactions Slab Absorption Slab Absorption and Scattering Semi-Infinite Medium January 2004 Chuck DiMarzio, Northeastern University Two-Layer 10471-2-8 Lecture Overview • • • • • Optical Spectrum and Sources Interaction of Light with Matter A Bit of Geometric Optics A Bit of Physical Optics Microscopes (1) – Point-Spread Function – Optical Transfer Function • Detectors and Cameras January 2004 Chuck DiMarzio, Northeastern University 10471-2-9 Camera Lens Image Location F’ A A’ F f f’ s’ s Lens Equation January 2004 Chuck DiMarzio, Northeastern University 10471-2-10 Camera Lens Magnification x A -x’ A’ s’ s m January 2004 x' s' x s Chuck DiMarzio, Northeastern University 10471-2-11 Field of View Film= Exit Window January 2004 Chuck DiMarzio, Northeastern University 10471-2-12 Diffraction x1 x What is the light distribution in the spot? January 2004 Chuck DiMarzio, Northeastern University 10471-2-13 Fraunhofer Diffraction January 2004 Chuck DiMarzio, Northeastern University 10471-2-14 Point Spreadand Optical Transfer Functions Fourier Transform object scale perfect image actual image January 2004 x’ SCALE PERFECT IMAGE x’ x MULT OTF ACTUAL IMAGE Chuck DiMarzio, Northeastern University d0 Convolve with PSF OBJECT R 10471-2-15 Fraunhoffer Examples January 2004 Chuck DiMarzio, Northeastern University 10471-2-16 Coherent and Incoherent OTF • Incoherent Image – Irradiance rather than field. – PSFinc is |PSFcoh|2 • Fourier Transform – OTFinc is |OTFcoh|2 – Meaning of Phase is a bit complicated January 2004 Chuck DiMarzio, Northeastern University 10471-2-17 1 0.9 0.8 0.7 An Extreme Example 0.5 Colorbar for all Image Object 20 0.6 40 0.4 0.3 0.2 0.1 60 20 80 40 20 100 60 40 120 80 60 140 100 80 160 120 100 180 20 40 60 80 Point-Spread Function of System January 2004 100 140 120 0 120 140 160 180 160 140 180 20 40 60 80 100 160 120 140 160 180 180 20 40 Chuck DiMarzio, Northeastern University 60 80 100 120 140 160 10471-2-18 180 Bright-Field Microscopy A’ F A F’ f s f’ s’ Object Plane January 2004 Image Plane Chuck DiMarzio, Northeastern University 10471-2-19 Lecture Overview • • • • • Optical Spectrum and Sources Interaction of Light with Matter A Bit of Geometric Optics A Bit of Physical Optics Microscopes (1) – Point-Spread Function – Optical Transfer Function • Detectors and Cameras January 2004 Chuck DiMarzio, Northeastern University 10471-2-20 Semiconductor Detector Conduction Band Absorption Emission Valence Band 10057p1-2 here hn eJanuary 2004 Chuck DiMarzio, Northeastern University 10471-2-21 Remember N-FET Structure? S, Source G, Gate n+ D, Drain n+ SiO2 Insulator 20-100mm Channel: 2 to 500 mm into page P-Type Material NMOS Metal-Oxide-Semiconductor January 2004 B, Body Chuck DiMarzio, Northeastern University Channel Length 1 to 10 mm 10471-2-22 Charge-Coupled Device (CCD) S G D S D B Channel Length 1 to 10 mm January 2004 B ~10 mm X nRows Chuck DiMarzio, Northeastern University 10471-2-23 CCD Charge Transfer Clock Voltage 1 0.9 2 Row Number 0.8 4 0.7 0.6 m10057_1.m Figure 1 6 8 0.5 0.4 0.3 10 0.2 0.1 12 0 0.5 V 1 1.5 2 time, Clock Cycles V 2.5 0 One Line Clock Signals January 2004 3 time Chuck DiMarzio, Northeastern University 10471-2-24 Computer Interfacing • Analog Camera and Frame Grabber • Digital Camera Digital Camera Analog Camera Computer with Frame Grabber Analog Monitor January 2004 Computer Monitor Chuck DiMarzio, Northeastern University Computer Computer Monitor 10471-2-25 Linearity and AGC 1 y Gx 0.8 Output Voltage • Automatic Gain Control (AGC) • Feedback 0.5 0.6 0.4 1 – Control G – Based on... 2 • Peak Signal • Average Signal • Peak in a Region 0.2 0 0 0.2 January 2004 0.4 0.6 Input Voltage 0.8 1 • Not Desirable for Quantitative Work Chuck DiMarzio, Northeastern University 10471-2-26 Pixelation and Digitization 255 Count 0 “Brightness” January 2004 Chuck DiMarzio, Northeastern University 10471-2-27 Digitization and Dynamic Range 2N-1 Saturation Maximum Signal Minimum Signal Step Size Pedestal Signal Voltage 0 January 2004 Dark Chuck DiMarzio, Northeastern University 10471-2-28 Some Standard and Extreme Parameters • VGA Frame Size: 640 by 480 – Up to 4k Square? • Standard Update Rate: 30 Hz. Interlaced – Up To few kHz. • Standard Digitization: 8 Bits – Up To 12. • Pixel Size: 10 micrometers. • Color Camera: 3 Channels, 8 Bits Each January 2004 Chuck DiMarzio, Northeastern University 10471-2-29