Register Number SATHYABAMA UNIVERSITY (Established under section 3 of UGC Act,1956) Course & Branch :M.Tech - W-BTE Max. Marks:80 Title of the Paper :Plant Cell and Animal Biotechnology Sub. Code :SBTX5006 (2010-11) Time : 3 Hours Date :01/10/2012 Session :FN _______________________________________________________________________________________________________________________________ 1. PART - A (6 x 5 = 30) Answer ALL the Questions Explain about in vitro fertilization and its applications 2. Write down the applications of transgenic plants in different areas 3. List out the methods involved in storage of animal cell lines 4. Narrate the methods of gene delivery in animal cell culture 5. Describe the types of plant tissue culture media and its uses 6. Give an account on haploid production PART – B (5 x 10 = 50) Answer ALL the Questions 7. 8. 9. Explain in detail account on Plant derived vaccines. (or) Describe the application of tissue culture in crop improvement for agriculture. List out the methods involved in measurement of cell growth and its advantage. (or) 10. Write short notes on: (a) Primary cell culture (b) Secondary cell culture 11. How the secondary metabolites produced from the callus in large scale? (or) 12. With a neat diagram, explain in details about Micropropagation and its application. 13. What is stem cell culture? Explain in details about method of isolation of stem cells from human. (or) 14. Comment on Assisted Reproductive Technology (ART) and micro insemination. 15. Explain in brief on embryo culture and embryo rescue method in plants. (or) 16. Write short notes on: (a) Somatic embryogenesis (b) Artificial seeds