CH. 5
PROKARYOTIC GROWTH
TBL05_01: Some microbial record holders
Figure 05.01: Atacama Desert
© Photodisc/age fotostock
Figure 05.02a: Binary fisson of bacteria
Figure 05.02b: A false color TEM of a cell of Bacillus licheniformis
undergoing binary fission
© Lee Simon/Photo Researchers, Inc.
Figure 05.03: A skyrocketing bacterial population
Growth curve plotted in logs and
arithmatically
Figure 05.04: Bacterial growth curve
Figure 05.05a: Bacterial colony on blood agar
Courtesy of Dr. J. J. Farmer/CDC
Note the slimy colonies of
Salmonella typhi, denoting
capsules
Figure 05.05b: Salmonella typhi culture
Courtesy of CDC
Figure 05.06b: Bacillus anthracis spore
© Scott Camazine/Alamy Images
Figure 05.06c: Germinating spore
Courtesy of Janice Carr/CDC
Figure 05.07: The formation of a bacterial spore
BACTERIAL GROWTH IS
AFFECTED BY MANY
FACTORS
TBL05_01: Some microbial record holders
Oxygen can
support or hinder growth
Figure 05.09abc: Bacterial cultivation in different gas environments
© Scott Coutts/Alamy Images
Figure 05.10: The effect of oxygen on prokaryotic growth
Hyperbaric chamber
Most bacteria like to grow at a
neutral pH
• Optimal pH for growth
Figure 05.12a-e: The streak plate isolation method
Courtesy of James Gathany/CDC
Population growth can be
measured in several ways
• Turbidity
• Direct measurements
• Indirect methods
Counting Bacteria by MPN (most
probable number)
Figure 05.13: Direct counting procedure using the Petroff-Hausser counting
chamber
Counting bacteria by filtration
Counting bacteria by measuring
turbidity in a broth culture
Figure 05.14: The standard plate count
© R.A. Longuehaye/Photo Researchers, Inc.
BACTERIAL DEATH
Bacteria die at a constant rate
Death Curve