EGR 1200 Fall 2011 Automatic Blood Pressure Measurement Lab 4 Oscillation Signal Amplifier and Electronic Blood Pressure Measurement Electrical and Computer Engineering Villanova University General Overview of Lab 4 Purpose: The purpose of Lab 4 is to give you a feel for electronic circuit construction, characterization, and verification, which, in addition to design and simulation, are necessary aspects of electronic engineering. Method: We will apply the oscillometric method and implement an oscillation signal amplifier circuit to extract information from the pressure sensor signal. This will allow us to make an electronic measurement of blood pressure without any additional sensor(s). Major Procedures: I. Implementing the oscillation signal amplifier circuit. II. Setting up the digital oscilloscope to acquire signals. III. Acquiring signals using the oscilloscope. IV. Determining the blood pressure. I. Implementing the oscillation signal amplifier circuit 1. Build the circuit to correspond to the diagrams and photos shown in in the next few pages. 2. Use color wires as follow: RED for +9V, BLACK for -9V, GREEN for 0 V (GND), and YELLOW for pressure signal from input to output of the amplifier. 3. Double check that the electrolytic caps have their negative terminal () at the lower potential. That is, for the capacitor across the +9V node, the capacitor’s negative terminal (-) is connected to 0 V. For the -9V node, its capacitor’s negative terminal (-) is connected to -9V and the positive terminal (+) is connected to 0 V. 4. You can either use the resistor color code or an ohmmeter to determine the resistance value for the resistors R1, R2, and R3.* 5. Show the completed circuit construction to your instructor/TA before proceeding further. *See the link below for resistors and the color code: http://samengstrom.com/nxl/3660/4_band_resistor_color_code_page.en.html Oscillation Signal Amplifier Circuit Schematic Resistors:R1, R2, & R3 Electrolytic caps: C1, C2, C3, & C5 Ceramic cap C4 -9 VDC (Black wire) (Yellow wire:Input) Op-amp (Yellow wire: Output) +9VDC (Red wire) 0 VDC (GND) (Green wire) 4 Oscillation Signal Amplifier Circuit Breadboard Diagram 5 Oscillation Signal Amplifier Circuit Breadboard Photo NEGATIVE SIDE of CAP C2 C3 C5 R3 R1 C4 Connector Location from Pressure/Power Supply Board Oscillometric Signal R2 LM324 U1A +9V (Red) 0V (Green) Pressure Signal -9V (Black) NEGATIVE SIDE of CAP C1 6 Oscillation Signal Amplifier Circuit (Zoom in at Op Amp Chip) Oscillometric Signal R1 R3 R2 LM324 U1A 1 2 3 4 Notch 11 C4 Pressure Signal 7 II. Setting up the digital oscilloscope to acquire signals 1. 2. 3. 4. 5. 6. 7. On the oscilloscope probes, slide the yellow switches to the 10X position to set the probe attenuation. Press the AUTO button to start the oscilloscope at a default state. Press the CH 1 button of the VERTICAL system. Verify that under the display word “Probe” is the text indicating “10X.” This setting indicate that we are using a 10X probe as set in step 1 above. Press the CH 2 button of the VERTICAL system. Verify that under the display word “Probe” is the text indicating “10X.” Turn the SCALE knob of the HORIZONTAL system to adjust the time scale to 1.0 s/div. The display should show “Time 1.000s.” Press the CH 1 button of the VERTICAL system. Turn the SCALE knob to adjust the voltage per division for channel 1 to 0.5 V/div. The display should show “CH1 500 mV.” Press the CH2 button of the VERTICAL system. Turn the SCALE knob to adjust the voltage per division for channel 2 to 1.0 V/div. The display should show “CH2 1.00 V.” II. Setting up the digital oscilloscope to acquire signals 8. Press the CH 1 button. Select the gray softkey next to the displayed word “Coupling” and keep pressing until “GND” is highlighted. GND, which stands for ground, will be our reference point. Now, using the POSITION knob within the VERTICAL system, adjust the knob such that the display shows “POS=0.00mV.” Press the CH 1 button again and using the same gray softkey, press until the word “DC” is shown. This DC indication tells us that we are directly coupling the pressure signal to the scope. 9. Press the CH 2 button and repeat the step above for channel 2. By setting both CH 1 and CH 2 GND to the center of the vertical display, we are setting the reference level for our signal to the center of this display. This then tells us that any signal waveform above the vertical center is a positive voltage and any signal waveform below is a negative voltage. 10. Press the MENU button of the HORIZONTAL system. Under the display word “Time Base,” set the time base to “Roll.” 11. Press the RUN/STOP button to put the oscilloscope in RUN mode. 12. Show your oscilloscope setup to your instructor/TA before proceeding further. III. Acquiring the signals using the oscilloscope Use the diagram and photos on the next page to aid with steps 1-3 below. 1. Connect the white tubing from the cuff to the pressure sensor on the backside of the pressure sensor/power supply board. 2. Attach the oscilloscope probes to the oscillation signal amplifier circuit breadboard. a. Connect CH 1 probe to the test point for Pressure Signal. b. Connect CH 2 probe to test point for the Oscillometric (Oscillation) Signal. 3. Connect the pressure sensor/power supply board to your board using the ribbon connector. Pin 4 of the connector should be in the same row as the Yellow Wire for Pressure Signal. Move the board’s switch to the ON position. 4. Place the cuff securely on your arm as done in Lab 1 Manual Blood Pressure Measurement and then press the START on the OMRON blood pressure meter to start inflating the cuff. 5. Press the RUN/STOP button to stop the signal acquisition. Make sure that the oscilloscope has acquired sufficient number of peaks of the oscillation signal after the maximum peak had appeared. Pressure Sensor Board to Breadboard Connections Ribbon Connector Pressure Signal from Cuff Pin 1 MPX5050 Pressure Sensor BACK FRONT (with connect to Breadboard) 11 Pressure Sensor/Power Supply Board Schematic (Red wire) (Black wire) (Yellow wire) (Green wire) 12 Measurement Setup Digital Oscilloscope: Captures pressure and oscillometric signals Omron BP Meter: Auto Inflate/Deflate Cuff Pressure Sensor & Power Supply Board: Outputs pressure (electrical) signal and supplies +9,-9 volts DC Breadboard of Amplifier/Filter Circuit to extract oscillometric signal from pressure sensor 13 IV. Determining blood pressure 1. 2. 3. 4. Push the Cursor button in the MENU system. On the display, using the gray softkeys choose “Track” for the “Mode”, “CH1” for “Cursor A”, and “CH2” for “Cursor B.” Notice also on the display under “CurA—” and “CurB—” there is a symbol of a clockwise rotation. This symbol refers to the knob to the left of the MENU system panel. If the symbol is highlighted under “CurA—,” for example, that means turning this knob moves cursor A. To select cursor B to correspond to the knob, push the gray softkey next to “CurB--.” Using Cursor B find the maximum valid peak for the oscillation signal. Record the voltage value displayed by the Cursor B. Select Cursor A and move it to the time position and record its voltage value. This latter value represent the mean arterial pressure in terms of voltage. Multiply the maximum peak recorded value by 0.8 and 0.9 to obtain the peak values for the systolic and diastolic pressures, respectively. Turn the POSITION knob in the HORIZONTAL system to locate peaks on the oscillation signal that closely match the calculated values from above. As you find a closest peak, record its value and the corresponding voltage value on Cursor A. This latter value represents the magnitude of the pressure signal in voltage. Appendix: Saving the signals to file You can save the display plots onto a bitmap file. To do so, 1. Press the STORAGE button in the MENU system. 2. Select the gray softkey to the right of the display word “Storage.” Keep pressing the button until “Bit Map” is highlighted. 3. Select the gray softkey to the right of the display word “External.” This will allow you to save to a USB key drive. 4. A new set of display words are shown. Press the gray softkey to the right of the display word “New File.” 5. After a new window popped up, type in the filename. Turn the knob with the clockwise symbol to move between the characters and press the knob to select the desired character. 15 Lab 4 Sensors and Analog Electronics Instructor/TA Sign Off Sheet & Report Form 1. 2. 3. 4. Breadboard construction successfully completed:_____________________________ Oscilloscope properly configured for measurement:___________________________ Pressure and oscillometric signal successfully captured:________________________ Measurements verified:_________________________________________________ Measured Max. Osc. Peak Amplitude (V) Calculated Osc. Peak Amplitude (V) Measured Osc. Peak Amplitude (V) Corresponding Measured Pressure Signal (V) Measured Pressure Signal (mm Hg) Mean Arterial Pressure Systolic Pressure Diastolic Pressure 5. Based upon your implementation of the oscillometric method, discuss the advantages and disadvantages of this method.