Osmosis Learning Objective: In order to be successful in this lesson you must be able to: Distinguish between facilitated diffusion and diffusion Osmosis define osmosis in terms of water potential explain the effect of osmosis on plant and animal cells Interpret data from experiments on osmosis and use it to find the concentration of solutions inside cells Fluid Mosaic Model Intrinsic Protein Carbohydrate Glycolipid Glycoprotein A C O2 B Fatty acids Phospholipid F Bilayer E Extrinsic Protein D Phosphate CO2 Free Template from www.brainybetty.com Cholesterol 3 Which is not a function of cell surface membranes? 1) Forms a boundary between cell surface and the environment that can control the substances that enter and leave the cell 2) Allows different conditions to be established inside and outside the cell 3) Allows cells to communicate with each other 4) Provides surfaces on which reactions can occur Name the formation that is created when phospholipids are shaken in water. Which is not a function of the phospholipid bilayer? 1) Allow lipid soluble substances to enter and leave the cell 2) Prevent water soluble substances entering and leaving the cell 3) Make the membrane self flexible and self sealing 4) Provide structural support Which is a function of extrinsic proteins? 1. To form channel proteins to allow water soluble ions to diffuse across the cell membrane 2. To form carrier proteins that bind to molecules like glucose and change shape to move them across the membrane 3. Provide structural support 4. To prevent leakage of water and dissolved ions from the cell Which is not a function of cholesterol? 1) Act as recognition site 2) Reduce lateral movement of other molecules including phospholipids 3) Make the membrane less fluid at high temperatures 4) Prevent leakage of water and dissolved ions from the cell Is cholesterol more common in plant or animal cells? Why? Which does a glycolipid consist of? Which is not a function of a glycolipid? 1) Act as recognition site 2) Maintain stability of the membrane 3) Help cells attach to one another to form tissues 4) Act as channels transporting water soluble substances across the membrane What does a glycoprotein consist of? Explain the reasons that the fluid mosaic model of the cell surface membrane is called the fluid mosaic model Which is not a function of cell surface membranes? 1) Forms a boundary between cell surface and the environment that can control the substances that enter and leave the cell 2) Allows different conditions to be established inside and outside the cell 3) Allows cells to communicate with each other 4) Provides surfaces on which reactions can occur Name the formation that is created when phospholipids are shaken in water. Micelles Which is not a function of the phospholipid bilayer? 1) Allow lipid soluble substances to enter and leave the cell 2) Prevent water soluble substances entering and leaving the cell 3) Make the membrane self flexible and self sealing 4) Provide structural support Which is a function of extrinsic proteins? 1. To form channel proteins to allow water soluble ions to diffuse across the cell membrane 2. To form carrier proteins that bind to molecules like glucose and change shape to move them across the membrane 3. Provide structural support 4. To prevent leakage of water and dissolved ions from the cell Which is not a function of cholesterol? 1) Act as recognition site 2) Reduce lateral movement of other molecules including phospholipids 3) Make the membrane less fluid at high temperatures 4) Prevent leakage of water and dissolved ions from the cell Is cholesterol more common in plant or animal cells? Why? • Animal cells- no cell wall to provide support What is your definition of osmosis? http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html OSMOSIS Movement of water through a selectively/partially permeable membrane from a region of higher water potential to a region of lower water potential. Water potential? What does that mean? Lower solute concentration Higher water concentration Higher water potential Higher solute concentration Lower water concentration Lower water potential Cell surface membranes and other membranes in the cell are partially permeable. They are permeable to water molecules and some other small molecules. They are not permeable to large molecules such as protein. Water potential is given the Greek letter psi. Y Water potential is the pressure exerted by water molecules in a solution. Pure water has a water potential of 0. Therefore all solutions have a water potential that is less than 0. That is, the water potential of a solution is a negative number. Water will pass by osmosis from a solution with a less negative water potential to a solution with a more negative water potential, through a partially permeable membrane. A B Solution A Y = -20kPa Solution B Y = -30kPa Solution A has a higher water potential than solution B. Water will pass from solution A to solution B through the partially permeable membrane. Partially permeable Visking tubing Sucrose solution Water The water has a higher Y than the sucrose solution. Water passes into the Visking tubing by osmosis. Weigh the Visking tubing before placing in the water and again after 20 mins. What will happen to the mass? Potato experiment • https://www.youtube.com/watch?v=GQAVND 5rtcw