WEBQUEST—Methods used for genetic biotechnology
Name ___________________
Date ___________
How are plasmids used to construct recombinant DNA
http://www.hhmi.org/biointeractive/genetic-engineering
What is a restriction enzyme?
http://www.phschool.com/science/biology_place/biocoach/red/cutting.html
Read the slide, then run all active links within the next few slides on the topic of restriction
enzymes.
What is the purpose for using a restriction enzyme for plasmid cloning
OR plasmid restriction mapping to verify correct construction of a
recombinant plasmid?
http://www.phschool.com/science/biology_place/biocoach/red/mapping.html run all active links
related to the topics.
What is electrophoresis and how does it work? Why is it used?
http://www.phschool.com/science/biology_place/biocoach/red/gel.html run all activated links
associated with the topic
What is DNA sequencing? How does it work? How do you read the
sequence? The old fashioned way—Sanger—to sequence DNA
www.hhmi.org/biointeractive/dna/DNAi_sanger_sequencing.html
The new way to sequence DNA : Shot gun sequencing
http://www.hhmi.org/biointeractive/dna/DNAi_shotgun_seq.html
How the human genome was sequenced
http://www.hhmi.org/biointeractive/dna/DNAi_human_genome_seq.html
What is PCR, and why has this technique revolutionized the study of
genetics?
http://www.hhmi.org/biointeractive/polymerase-chain-reaction-pcr
http://www.hhmi.org/biointeractive/polymerase-chain-reaction
What is a gene chip (microarray)? How is it made? Why are they used?
http://www.hhmi.org/biointeractive/gene-chip-manufacturing
http://www.hhmi.org/biointeractive/how-analyze-dna-microarray-data
http://media.hhmi.org/hl/02Lect3.html chapter 22-29
What is a genomic DNA library and how is it screened? Why are they
used?
http://www.sumanasinc.com/webcontent/animations/content/dnalibr
ary.html
What is a cDNA library and how is it screened? Why are they used?
http://www.maxanim.com/genetics/cDNA/cDNA.htm
What are the different types of cloning? How are they done? Why are
they used?
http://learn.genetics.utah.edu/content/tech/cloning/ run all the activities
What are all the different types of stem cells? How are they made?
What are the controversies for making and using stem cells? How are
they used for research and medicine?
http://learn.genetics.utah.edu/content/stemcells/ run all the applications
How does DNA fingerprinting work? Why is it done? How do you read
a DNA fingerprint?
http://www.teachersdomain.org/asset/tdc02_int_creatednafp2/
Watch all 6 of these animations associated with this chapter and fill in
any blanks remaining above, as well as gather info on southern blotting.
http://highered.mheducation.com/sites/0072556781/student_view0/c
hapter14/animation_quiz_3.html