Determine the repertoire of chromosome movement in segregation, repair and recombination Identify forces responsible for chromosome movement Is chromosome movement integral for DNA repair? What is the cellular response, including chromosome reorganization to DNA damage? KBY2501 (LacO integrated ~1.1Kb from CEN11) ~1.1 Kb LacO Repeat CEN11 (32mer) I III KBY2502 (LacO integrated ~12.7 Kb from CEN11) ~12 Kb LacO Repeat (32mer) CEN11 I III Comparison Timecourse of Chromosome Markers 1Kb and 25Kb from CEN3 9 8 Spot Separation Distance (um ) 7 6 5 CEN spot separation 4 25Kb from CEN3 spot separation 3 2 1 0 0:00:00 0:14:24 0:28:48 0:43:12 Elapsed Tim e (hrs:m in:sec) 0:57:36 1:12:00 1:26:24 Yeast chromosomes oscillate from pole to pole prior to anaphase onset. Centromeres separate prior to the chromosome arms Rate of separation of chromosome arms exceeds rate of centromere separation Mechanisms of cohesion and separation are likely to differ along the chromatin fiber Centromeres on the same chromatid attach to opposite poles: Chromosome Breakage Centromeres on the same chromatid attach to same poles: NO Chromosome Breakage Chromosome rearrangements visualized in time course from galactose to glucose Gal Glu Gal Glu 1100bp 865bp RAD+ Repair mutant rad52- or rad1- Single strand excision Repair via Single strand annealing Revealed by 1100 bp repair product Activate Dicentric Chromosome Does mid-anaphase delay precede break ? or mid-anaphase delay follows break Mid-anaphase pause is dependent upon RAD9 checkpoint gene Mid-anaphase pause is not dependent upon the spindle assembly checkpoint genes, MAD2 or BUB1 RAD9 recognizes DNA damage and/or chromatin alterations Does RAD9 “mobilize” chromosomes/repair machinery in response to DNA damage Viability % 80 70 60 50 40 30 20 10 0 ka r9 p3 ki dy ne in u7 0 yk ra d1 ra d5 2 ra d9 W ild ty pe Viability % Viability of cells upon activation of the dicentric chromosome Repair mutants (rad1, rad52 and Yku70), rad9 checkpoint and mt based motility mutants (dynein, kip3, kar9) The mid-anaphase pause is “longer” in the absence of cytoplasmic dynein or kip3 Cytoplasmic microtubule-based motor proteins contribute to the fidelity of chromosome repair Mt based motor proteins effect the DNA damage response How do “repair mutants” effect chromosome organization Ku- Non-homologous end-joining Physiological functions of Ku • Ku70, Ku80 knockouts in mice have similar phenotype to SCID – V(D)J defects arrest lymphocyte development • Ku70, Ku80 -/- mice are runts compared to +/littermates – Number of cell divisions in development limited by impaired ability to repair endogenously generated DNA damage – Ku-deficient cells might take longer to repair this damage • Ku80 -/- dams fail to nurture their pups Yeast Ku in telomere maintenance • Disruption of yKu70p and yKu80p genes affect telomeric silencing, telomere length maintenance and viability of cells containing dicentric chromosomes – inactivate Ku, lose telomeric silencing – inactivate Ku, shorten telomeres – inactivate Ku, decrease viability of cells containing dicentric plasmids • Ku clusters yeast telomeres to peripheral sites in nucleus – In diploids, telomeres usually found in 6-7 clusters around nuclear periphery – In Ku subunit mutants, more clusters in random locations Featherstone, C., and Jackson, S. Mutat Res. 1999 May 14;434(1):3-15. Review. Activation of dicentric chromosome in Ku mutants results in chromosome decondensation Chromosome decondenses in stepwise fashion Dicentric chromosome stretching in metaphase in a stepwise fashion and recoil upon spindle disassembly Regularity of spacing upon chromatin decompaction Chromosome breakage and retraction to spindle poles Sir2p binds to stretched lacO DNA MAT Chromatin stretching is recognized in vivo Chromatin can act as an in vivo tensiometer RAD9+ rad9- Rad9 is required for chromosome unfolding in response to activation of the dicentric chromosome Laboratory Projects Dale Beach- RNA localization Paul Maddox-Mt polarity and dynamics Elaine Yeh- Asymmetric protein determinants THANKS !!!