MAKING PUNNETT PREDICTIONS http://www.exploratorium.edu/exhibits/mutant_flies/mutant_flies.html IF GENE is AUTOSOMAL and RECESSIVE TO + MALES:FEMALES 1:1 F1 ___________________ F2 ___ = ___ ___ = ___ ___ = ___ ______________ ______________ _______ http://www.exploratorium.edu/exhibits/mutant_flies/curly-wings.gif IF GENE is AUTOSOMAL and DOMINANT TO + MALES:FEMALES 1:1 F1 ______________________ F2 ___ = ___ ___ = ___ ___ = ___ ______________ _______________ ______ F1 IF GENE is LETHAL (YY) (Can’t cross pure dominant) Males:females 1:1 F1 ___ = _________ ___ = _________ F2 F2 ___ = ___ _______ ___ = ___ _______________ ___ = ___ ________________ ______ ratio IF GENE is X-linked recessive Different pattern if gene is inherited from mom or dad Mutant mom X wild type dad F1 F1 ____________________ ____________________ _____________________ _____________________ _____________________ _____________________ When using Virtual fly lab Choose ignore sex and see if it changes the ratios IF GENE is X-linked recessive Different pattern if gene is inherited from mom or dad Wild type mom X mutant dad F1 F2 ______________________ _______________________ ______________________ _______________________ When using Virtual fly lab Choose ignore sex and see if it changes the ratios IF GENE is X-linked dominant MUTANT MOM X WILD TYPE DAD F1 ______________________ ________________________ F2 ________________________ ________________________ ________________________ When using Virtual fly lab Choose ignore sex and see if it changes the ratios IF GENE is X-linked dominant WILD TYPE MOM X MUTANT DAD F1 XM y X+ X+XM X+y X+ X+XM X+y ________________________ ________________________ X+ F2 y X+ X+X+ X+y XM X+XM XMy ________________________ ________________________ ________________________ ________________________ When using Virtual fly lab Choose ignore sex and see if it changes the ratios GENETIC ABBREVIATIONS DOMINANT MUTATIONS: first letter = capitalized. EX: sternopleural bristles= Sp RECESSIVE MUTATIONS = lower case EX: mahogany eyes = mah normal "wild type" version of the gene = same abbreviation as mutant with a "+" superscript For example, Sp+ and mah+. Sometimes, when the meaning is obvious, "+" by itself is used EX: heterozygote genotypes: +/Sp and +/mah. VIRTUAL FLY LAB DOESN’T FOLLOW THIS PROTOCOL: You need to explore and figure out whether it is dominant or recessive.