DNA REPLICATION
MAIN IDEAS

What exactly happens in the S phase? How does this occur?

What does 3’ and 5’ stand for?

Looking at the website, does replication go in one- way direction? If
yes, which direction?

What does semi- conservative mean?

What is the difference between the leading strand and lagging
strand?

At the end of replication, how do the strands compare with each
other?

USING YOUR NOTES, YOUR MEMORY AND THE WEBSITE BELOW, TRY
AND ANSWER THE ABOVE QUESTIONS:
https://lpscience.fatcow.com/jwanamaker/animations/DNA%20Re
plication%20-%20long%20.html
THE 5 W’s of Replication:

What are the 5W’s in DNA REPLICATION:

WHO is involved (are the major protein players)?

WHAT is being accomplished:

WHEN does it occur in the cell cycle:

WHERE does it occur in prokaryotes vs eukaryotes?


EUKARYOTIC CELLSWHY does replication happen:
PROKARYOTIC CELLS-
THE 5W’s in DNA REPLICATION:

WHO is involved (are the major protein players): Helicase, SSB, DNA
Polymerase, Primase, ligase

WHAT is being accomplished: DNA REPLICATION

WHEN does it occur in the cell cycle: S PHASE

WHERE does it occur in prokaryotes vs eukaryotes?


EUKARYOTIC CELLS- NUCLEUS
PROKARYOTIC CELLS- cytoplasm
WHY does replication happen: TO MAKE MORE DNA (FOR MITOSIS)
HOW does DNA REPLICATION occur????

http://www.bioteach.ubc.ca/TeachingResources/MolecularBiology
/DNAReplication.swf

http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/dn
a-rna2.swf
MAJOR PLAYERS:
WHAT DO THEY DO???
Helicase
SSB
DNA polymerase
RNA primase
Ligase

NOTES: FINISH DNA notes and collect (overview)//

ACT OUT…

VISUALIZE: AMEOBA SISTERS REPLICATION:
https://www.youtube.com/watch?v=5qSrmeiWsuc

DNA REPLICATION PAPER CLIP//PIPE CLEANER ACTIVITY

MUTATIONS …