Valencia College
MCB 2010C MICROBIOLOGY
CRN 23584 Spring 2015 (Jan12 – May 3)
Professor
Nalini Odapalli
Office
HSB117
Phone
330 789 2824 (Cell) 407 582 1809 (Office)
Email
nodapalli@mail.valenciacollege.edu
Office Hours
In office: Monday 10:00 am – 11:30 am; Wednesday 10:00 am- 01:00 pm;
Thursday 02:00 pm – 03:30 pm;
By email/text:
Friday 12:00 pm – 06:00 pm; Saturday 12:00 pm – 01:00 pm
Course
Microbiology - 4 credit hours
Class
Tuesday - Bldg 1-230
Thursday- AHS 317
10:00- 12:45 pm (Lecture)
10:00- 12:45 pm (Lab)
COURSE DESCRIPTION This lecture/lab course is designed for Health Science majors. Survey of microbial forms
with emphasis on bacteria and viruses: morphology, physiology, genetic mechanisms, and control of
microorganisms. Pathogenic processes and microbes discussed in detail.
PREREQUISITE
Minimum grade of C in BSC 1010C or BSC 2093C
PREREQUISITE NOTE
TEXT
If you do not meet the prerequisite requirements for this course, please arrange for a
conference with your instructor as soon as possible. It is not advisable to take BSC 2093C
and MCB 2010C in the same term unless you have a strong science background.
Cowan, Marjorie K. Microbiology: A Systems Approach. 4th ed.
LABORATORY MATERIALS Leboffe, Michael J. and Burton E. Pierce. Microbiology Laboratory Theory & Application. 2nd ed.
Additional lab materials - Lab coat placed in a gallon-sized freezer bag, disposable gloves that fit to the hand
(minimum of two pairs per lab), black permanent ink marking pen, closed-toe shoes, and a hair tie-back if appropriate.
ASSESSMENT
Lecture Tests
Final Lecture Exam
Lecture Quizzes
Laboratory Quizzes
Laboratory Practicals (200 pts each)
Total Points
400
100
100
100
400
1,100
Note: Any uncompleted coursework will be assigned a grade of 0.
GRADING SCALE
A 90 - 100 %
B 80 - 89 %
C 70 - 79 %
D 60 - 69 %
F 59 - 0 %
Lecture Tests and the Final Lecture Exam will cover the material presented from the point of the previous test. A
lecture quiz will be given once a week at the beginning of class. If you are late, you will not be permitted to take
the quiz. You must be present for the complete class for your quiz grade and exam grade to be counted.
A lab quiz will be given at the beginning of each lab. If you are late, you will not be permitted to take the quiz. You
must be present for the complete lab for your quiz grade to be counted. Two laboratory practicals, a midterm and
a final, contain actual specimens collected from laboratory exercises. Answers to questions following each
laboratory exercise are potential questions for lab practicals.
Read labs before coming to lab. Lack of preparation or poor technique may cause a loss of up to 10 points from
the laboratory work grade. In the case of repeated safety violations, a student may be excused from the lab.
CLASS ATTENDANCE AND MAKE-UP WORK
Attendance is marked at the beginning of each class. If you are
late even by a second you will be marked tardy. In this class a student missing 4 hours of class time or who
has 3 late entries or 3 early exits will be withdrawn from the class. Before withdrawing from the class you are
advised to consult the professor and an advisor or counselor. The drop/refund deadline 1/20/2015. The deadline
for withdrawal (W grade) from this course is 03/27/2015.
Absences will be excused with proper documentation of an unexpected life event. If you have perfect attendance,
a 69, 79 or 89% following the final exam, your grade will be rounded up but otherwise no rounding will occur.
Make-up policy: You can make up only lecture exams on the final lecture exam day. You cannot make up quizzes
and lab exam whether you arrived late or were absent.
DISCLAIMER STATEMENT
Changes in this syllabus and/or schedule may be made at any time during the term by notice of the professor. It is
your responsibility to stay informed of any changes. You are required to keep all returned graded work for two
weeks after a final grade has been issued to ensure that no grade discrepancies have occurred. This syllabus is a
contract between the student and the instructor. By attending this course, the student agrees to and accepts the
terms and conditions of this contract. It is the responsibility of the student to carefully read this syllabus/contract and
to adhere to all policies and procedures within.
ACADEMIC HONESTY All forms of academic dishonesty are prohibited at Valencia College. Academic dishonesty
includes, but is not limited to, plagiarism, cheating, furnishing false information, forgery, alteration or misuse of
documents, misconduct during a test situation, and misuse of identification with intent to defraud or deceive.
Academic penalties may include, without limitation, one or more of the following: loss of credit for an assignment,
examination, or project; withdrawal from course; a reduction in the course grade; or a grade of “F” in the course. If a
student is found cheating, notification will be submitted to the Dean of Science and record of the violation will be
added to your permanent transcript.
During tests, all cell phones and electronic devices should be turned off and stored away in purses or backpacks. Book
bags, backpacks, purses, notes, drinks and/or food are not allowed in the testing area. You will need only a pencil and
eraser. Students will not be permitted to leave the room and come back to finish their test under any circumstances
unless medical documentation has been submitted.
FINAL EXAM Students not taking the final exam will receive 0 points for that assignment, and the 0 will be
averaged in as part of the final grade.
WITHDRAWAL POLICY
If you withdraw from class before the withdrawal deadline, you will receive a
“W.” Students are not permitted to withdraw themselves after the withdrawal deadline. However, you may be
withdrawn up to the beginning of the final exam period for violation of the class attendance policy in which case
you would receive a “W.” Any student who withdraws or is withdrawn from a class during a third or subsequent
attempt will be assigned a grade of “F.” You are allowed 2 attempts at the regular tuition rate, after which you will
be required to pay out-of-state tuition.
STUDENT CONDUCT
By registering at Valencia Community College, a student assumes the responsibility for
becoming familiar with and abiding by the general rules of conduct. Violation of any of these rules may lead to
disciplinary warning, probation, suspension, expulsion, or other appropriate and authorized penalty. The Student
Code of Conduct is published in the Valencia Student Handbook.
-No walking in and out of the classroom/lab frequently
-If you have questions then raise your hand and ask professor not your neighbor
-Recording lectures is allowed but the privilege will be taken away depending on student behavior
-Be on time and stay till the end of the class for complete credit
COLLEGE POLICIES
A full description of all College policies can be found in the College Catalog at
http://www.valenciacollege.edu/catalog/ ; Policy Manual at http://www.valenciacollege.edu/generalcounsel/;
and the Student Handbook at http://valenciacollege.edu/studentdev/CampusInformationServices.cfm
COMPUTER/EQUIPMENT USE Use of computers in the Business, IT, and Public Service classrooms at Valencia
Community College is restricted to those activities designated by the instructor to enhance the class materials.
Any other use is strictly forbidden. Inappropriate use includes, but is not limited to:
Use of computer to send Email or access Internet sites not specifically assigned in class.
Use of computer for job, internship, or other activities not assigned in class.
Modifying any hardware or software system configuration or setting.
Activities not in accordance with the Valencia Student Code of Conduct.
Use of computers in the departmental open lab is limited to those activities involved with preparing homework or
coursework in this department and is subject to the same restriction as listed above. Computer use is remotely
monitored; any student using computers inappropriately may be subject to dismissal from class or banishment
from the lab. Subsequent offense may be sent to the campus administration for further disciplinary action.
STUDENT ASSISTANCE PROGRAM
Valencia College is interested in making sure all our students have a
rewarding and successful college experience. For that purpose, Valencia students can get immediate help that
may assist them with psychological issues dealing with stress, anxiety, depression, adjustment difficulties,
substance abuse, and time management, as well as relationship problems dealing with school, home or work.
Students have 24-hour unlimited access to the BayCare Behavioral Health’s confidential student assistance
program phone counseling services by calling (800) 878-5470. Three free confidential face-to-face counseling
sessions are also available to students.
STUDENT SERVICES
Tutoring Center: Bldg 7: 240 (Phone: 407 582 1633)
Testing Center: Bldg 7: 217 (Phone 407 582 1323)
Please note that tutoring services are free and are available at all Valencia campuses.
STUDENTS WITH DISABILITIES Students with disabilities who qualify for academic accommodations must
provide a letter from the Office for Student with Disabilities (OSD) and discuss specific needs with the professor,
preferably during the first two weeks of class. The Office for Students with Disabilities determines
accommodations based on appropriate documentation of disabilities (SSB 102. Phone 407 582 1523. West
Campus).
CELL PHONES
Turn off or silence your cell phone during class. Text messaging during class is distracting
to others and does not allow the student to be mentally attentive. A student using cell phone will be asked to
leave the classroom. Cell phones should not be handled at all during lab and should be turned off and put away
due to safety concerns. Use of cell phone usage in class and lab will result in a loss of 10 points per incident.
In addition to specific microbiology objectives, the course will also reinforce the following competencies wholly or
partially:
VALENCIA COMMUNITY COLLEGE STUDENT COMPETENCIES
1. Think – Think clearly, critically, and creatively. Analyze, synthesize, integrate, and evaluate in many domains of
human inquiry.
2. Value – Make reasoned value judgments and responsible commitments.
3. Communicate – Communicate with different audiences using varied means.
4. Act – Act purposefully, reflectively, and responsibly.
LEARNING COURSE OUTCOMES
1. Students will be able to identify the major milestones in microbiology.
2. Students will be able to describe how macromolecules contribute to the functions necessary for microbial life.
3. Students will be able to classify microorganisms according to a common taxonomic hierarchy.
4. Students will be able to differentiate between prokaryotic and eukaryotic organisms and explain the function
of each structure in both cell types.
5. Students will gain an understanding of viruses by explaining viral structure, multiplication, spread and control.
6. Students will be able to explain basic microbial metabolism and summarize what is needed for the growth of
microorganisms.
7. Students will be able to describe all the chemical and physical means available to control microbes and to
identify those that provide sterility.
8. Students will be able to identify the mechanism of action of commonly used antimicrobial drugs and the
concerns associated with the development of drug resistant strains.
9. Students will model their knowledge of molecular genetics and recognize the importance of basic genetic
engineering.
10. Students will be able to demonstrate the appropriate use of epidemiological terminology in context of public
health.
11. Students will be able to identify the mechanisms of pathogenicity and distinguish the different levels of
immunity operating in the human body.
12. Students will be able to identify the causes of major human infectious disease and relate that information to
the appropriate organ system.
13. Students will demonstrate a working knowledge of aseptic technique.
14. Students will demonstrate an understanding of microscopy.
15. Students will integrate their lab knowledge to identify unknown microbes and determine how appropriate
antimicrobial drugs are chosen for given bacterial infections.
Grade tracking table
Date
Exam/ Quiz
Total points
My Score
Measures to improve grade
Microbiology Schedule
Spring 2015
Date
Lecture
Date
Lab (LAB COATS REQUIRED)
Jan 13
Introduction – Ch. 1
Tools of the Lab – Ch. 3
Jan 15
Introduction to the Microbiology Lab
Jan 20
Bacteria and Archaea – Ch. 4
Eukaryotes – Ch. 5
Viruses – Ch. 6; Quiz in Chap 6
Jan 22
Culturing Bacteria and Using the Microscope
Jan 27
LECTURE TEST 1: Ch. 1; 3-5
Genetics – Ch. 9
Jan 29
Staining I
Feb 3
Genetic Engineering – Ch. 10
Feb 5
Staining II
Feb 10
Controlling Microbes – Ch. 11
Antibiotics – Ch. 12
LECTURE TEST 2: Ch. 9-12
Feb
12
Physical Growth Factors for Bacterial Growth
Infection and Disease – Ch. 13
Nonspecific Defenses – Ch. 14
Specific Defenses – Ch. 15
Feb 24, Specific Defenses – Ch. 15
Mar 3, Disorders in Immunity – Ch. 16
Mar 5
LECTURE TEST 3: Ch. 13-16
Mar 17 Diagnosing Infections – Ch. 17
Diseases of the Skin/Eyes – Ch. 18
Feb
19
Introduction to Biochemical Test Media
Feb
26
MIDTERM LAB PRACTICAL EXAM WEEK
Materials come from laboratory experiments
performed during weeks 1-5
Mar
19
Culture and Sensitivity (C&S)
read page 455
Mar 24
Nervous System Disease – Ch. 19
Cardio/Lymph Disease –Ch. 20 (Quiz)
Mar
25
C&S: Unknown Identification Lab and Antibiotic
Selection
Mar 31
LECTURE TEST 4: Ch. 17-19
Respiratory Disease - Ch. 21
Apr 2
DNA Lab
Apr 7
Apr 9
Immunology lab
Apr 14
Digestive System Disease– Ch. 22
Genitourinary Diseases – Ch. 23
Genitourinary Diseases – Ch. 23
Apr 21
Apr 28
Presentations (Ch. 20 – 23)
Final exam Ch 21-23
Apr 23 FINAL LAB EXAM
Feb 17
Apr 15 Human Microbiology/Epidemiology Simulation Lab
Analysis
Exam on lab experiments performed during weeks
6-12
Changes in syllabus and/or schedule may be made at any time during the term by notice of the professor. It is
your responsibility to stay informed of any changes. You are required to keep all returned graded work for two
weeks after a final grade has been issued to ensure that no grade discrepancies have occurred.
Classes do not meet on the following dates: Jan 19, March 9 to March 15.
Final grades due: May 4, 2015 9:00 AM.
MICROBIOLOGY (MCB 2010C) LABORATORY EXERCISES
Valencia College – Spring 2015
Week 1. Lab Introduction
- Safety and laboratory Guidelines: students read pp. 1-6
- “Scavenger hunt” (introduce students to location of lab’s safety equipment)
- Exercise 1-1 Glo-Germ™ Hand Wash Education System (optional)
- Exercise 2-1 Ubiquity of Microbes
- Exercise 3-1 Introduction to the Light Microscope
- learn parts, use, care and storage of the microscope; crossed thread and letter “e”
prepared slides,
- Exercise 3-3 Examination of Eukaryotic Microbes (begin observing prepared slides)
Week 2. Culturing Bacteria and Using the Microscope
- Analysis of last week’s experiment
- Students read Exercise 1-2 about Nutrient Agar and Nutrient Broth Preparation,
page 19, and pp. 59-66 about Colony Morphology
- Exercise 1-4 Common Aseptic Transfers and Inoculation Methods
- Exercise 1-5 Streak Plate Methods of Isolation
- Exercise 2-11 Steam Sterilization (“field trip demo” to see and explain the principles of an
autoclave) (optional)
- Exercise 3-1 Introduction to the Light Microscope (cont.)
- Exercise 3-3 Examination of Eukaryotic Microbes (finish observing prepared slides)
Week 3. Staining I
- Analysis of last week’s experiments
Students read pp. 69 and 73 about Growth Patterns on Slants and Broth
Students read pp. 153-158
- Exercise 3-4 Simple Stains
- Exercise 3-5 Negative stains
(Optional- assess each student’s ability to properly clean the microscope)
- Exercise 3-10 Wet Mount Preparations – Live protist samples or pond water samples from Lake
Pamela (Students read page 143)
Week 4. Staining II
- Exercise 3-6 Gram Stain (optional assessment of student ability to observe stained bacteria
under the oil immersion lens)
- Exercise 3-7 Acid-Fast Stain (prepared slides for observation)
- Exercise 3-9 Endospore Stain (prepared slides for observation)
Selective and Differential Media - Students read pp. 77, 207 and 237
- Exercise 4-3 Mannitol Salt Agar
- Exercise 4-1 Phenylethyl Alcohol Agar
- Exercise 4-2 Columbia CNA with 5% Sheep Blood Agar
- Exercise 4-5 Eosin Methylene Blue Agar
- Exercise 4-6 Hektoen Enteric Agar
- Exercise 4-4 MacConkey Agar
Week 5. Physical Growth Factors for Bacterial Growth
- Analysis of last week’s experiments
Students read pp. 77, 207 and 237
- Exercise 2-6 Fluid Thioglycollate Medium
- Exercise 2-8 The Effect of Temperature on Microbial Growth
Bacterial Nutrition
-Exercises 5-11; 5-13; 5- 16
Week 6. Introduction to Biochemical Test Media
- Analysis of week 5 experiments
- Exercises 5-4; 5-5; 5-6; 5-9; 5-10; 5-19; 5-20; 5-21; 5-23
Week 7. MIDTERM LAB PRACTICAL EXAM WEEK
- Materials come from laboratory experiments performed during weeks 1-5
- Analysis of week 6 experiments (You also have the option of doing this during week 8, before
the C&S inoculation labs)
There will be NO scheduled labs on March 3rd – 7th as this will allow all the labs to be on the same
schedule after Spring Break. Instructors may use this time to lecture, if so desired.
Week 8.
Culture and Sensitivity (C&S)
Students read page 455
- Exercises 5-2; 5-3; 5-7; 5-8; 5-12; 5-14; 5-15; 5-18
- Exercise 9-1 Identification of Enterobacteriaceae
- Exercise 7-2 Antibiotic Susceptibility Test (Kirby-Bauer Method)
Week 9. C&S: Unknown Identification Lab and Antibiotic Selection
Students read pp. 501-508 (API 20 E) and pp. 509-516(Enterotube II)
- Exercise 9-1 Identification of Enterobacteriaceae (analysis)
- Exercise 7-2 Antibiotic Susceptibility Test (Kirby-Bauer Method) (analysis)
Week 10. DNA Lab
- Exercises 2-12 and 8-2 The Lethal Effect of Ultraviolet Light on Microbial Growth
-instructors read handout to see how this lab will be performed differently from the book
- Exercise 8-3 Bacterial Transformation: the pGLO System
Week 11. Immunology lab
- Analysis of last week’s experiments
Students read page 391
- ELISA Test (HIV simulation test)
- Latex Agglutination Assay
- Exercise 7-1 Snyder Test
- Exercise 7-4 Epidemic Simulation (Influenza party)
Week 12. Human Microbiology/Epidemiology Simulation Lab Analysis
- Analysis of previous lab’s experiment
- Exercise 6-4 Differential Blood Cell Count
Review
Week 13. FINAL LAB EXAM
- Exam on lab experiments performed during weeks 6-12
LABORATORY POLICIES
Your “lab designation” is SAM. The first letter represents the day of the week that your lab meets. The next two
letters are AM for a morning lab. You will be assigned a group number the first day of lab.
Any submitted work for lab or lecture should always include your lab designation and your lab group number
assigned the first day of lab. For example, you would always include SAM-6 on your work if you were assigned to
group 6. Failure to indicate your lab designation on your work may result in a one point penalty.
Always come to the lab prepared with your Lab Manual, the companion Photographic Atlas, lab coat, at least two
pairs of disposable gloves, and a black permanent marker. Shoes are required and long hair should be pulled back.
A gallon-bag or larger is required for your lab coat, marker, pen or pencil to be left in the lab for the term. Failure
to bring your supplies and to dress appropriately for lab will result in the loss of 1 point from your lab grade.
A lab quiz will be given at the beginning of each lab. If you are late and the lab quiz has already been
administered, you will not be allowed to take the quiz. You must participate in the entire lab to receive credit for
the lab quiz.
Lab safety and proper technique are of paramount importance; penalties may be accrued for failure to follow
instructions (up to 10 points). In the case of repeated safety violations, a student may be excused from the lab.
Food and drinks are not permitted in the lab. Cell phones and other electronic devices are not permitted to be
accessed during the lab without prior permission from the instructor. Cell phone use is considered to be a safety
violation and will cause the deduction of 1 point from your lab quiz grade. Lab coats and writing utensils should
not be removed from the lab without first autoclaving or disinfecting the surfaces. All lab coats will be autoclave
after the Lab Practical Final and returned to you the following week.
Recently, an outbreak of Salmonella in the Northeast was traced back to a teaching lab. A child of one student
became infected after handling her mother’s cell phone. Please understand that your safety is very important to
us and safety rules are in place to protect you and yours.
Safety in the Microbiology Laboratory
The following safety guidelines are to be adhered to at all times when performing exercises and other activities
contained in this lab manual. Gross negligence of the following safety practices will result in the reduction of your
laboratory grade by 10 points and possible expulsion from the lab.
If you are pregnant, immunocompromised or immunosuppressed, please contact your primary health care
provider to discuss your lab participation. Bring any concerns to your instructor.
1. Handling of electronic devices is not permitted in the lab. This includes iPods, MP3 players, cell phones,
and calculators. If you want to take pictures of cultures, please make arrangements with your instructor.
2. Do not eat, drink, smoke, store food, or apply cosmetics in the laboratory. You may take a short break to
eat and drink at the student lounge at your instructor’s discretion. Keep paper, pencils, fingers and other
objects out of your mouth.
3. Keep all aisles clear. Place all backpacks or purses under your desk or on the cart provided.
4. Keep the tops of the bench neat, clean, and free from wearing apparel and unnecessary texts.
5. Always wipe the working area of the lab bench with the disinfectant provided before and after every
laboratory period.
6. Wash your hands before and after every laboratory period. Wash your hands immediately and
thoroughly if they are contaminated with microorganisms. You may apply copious amounts of alcohol gel
on your hands as a final wash step.
7. Shoes are required for admittance to the lab. Open-toe sandals are not allowed. Baggy clothing, dangling
jewelry, shoes made of woven material, high heels, sandals and flip-flops are not appropriate.
8. Long hair should be tied back securely.
9. Lab coats must be worn at all times when working with microbes or when working near each others who
are working with these materials. Lab jackets are not to be construed as substitutes for appropriate lab
attire. Following current CDC and OSHA guidelines, laboratory coats are never to be worn outside the
confines of the laboratory.
10. A gallon-bag or larger is required for the following lab supplies: lab coat; permanent marker; pen or
pencil. These items are to be left in the lab for the term. If a spill should occur or your lab coat needs
washed, the lab coat must be autoclaved before it is removed from the lab.
11. Use aseptic technique at all times. Wear gloves when performing exercises. Do not hesitate to replace
gloves when torn or suspected of contamination. Gloves should be removed by using proper technique to
avoid auto-inoculation. Do not discard used gloves in the trashcan. Always place gloves in the orangelined biohazard containers under the biosafety hood.
12. Please be aware that improper handling of bacterial cultures may place you and others at risk for
infection. Cultures used in the lab experiments are highly concentrated. Handle all bacterial cultures with
caution.
13. Never carry test tubes without the tubes first being placed in a test tube rack.
14. Use mechanical pipetting devices (pipettors) when pipetting. Place pipettes in designated containers
after using. Do not lay them on the bench top or in the sink.
15. Call your Instructor in cases of culture spills. Cover the spill with paper towels, wet the towels with
disinfectant, and leave for 20 minutes. Using gloves, place the paper towels in a biohazard container.
16. Appropriately label all Petri plates with the microbe or microbial source, lab group, medium, date and
initials on the bottom of the Petri dish using a permanent marker. Label test tubes accordingly.
17. Place glassware and slides contaminated with cultures and related specimens in the designated
containers.
18. Do not perform unauthorized experiments, or play practical jokes or engage in any other behavior that
might confuse, startle, or distract anyone else. Do not use equipment without proper instruction.
19. Work only with your own body fluids in exercises requiring these specimens.
20. Dispose of waste and excess material in proper places:
a. Place used gloves in the orange-lined biohazard containers under the biosafety hood.
b. Place all used pipettes in designated containers.
c. Place Petri dishes in the orange-lined biohazard containers after evaluation.
d. Place test tubes in the designated test tube racks under the biosafety hood after evaluation.
e. Dispose of broken glass in the broken glass containers.
21. Turn off Bunsen burners and all other instruments after used including microscopes. Be sure to follow the
instructor’s directions on the proper way to store microscopes.
22. Know the locations of and the proper use of fire alarms, fire extinguishers, fire blankets, chemical fume
hoods, safety showers and emergency eye wash stations. Know the location of the phone in order to call
for help when necessary.
23. Notify your instructor immediately of any injury, spill, fire or explosion. ALL INJURIES AND ACCIDENTS,
REGARDLESS OF SEVERITY, MUST BE REPORTED. Clean-up non-hazardous spills immediately. Never clean
up a spill of hazardous material unless it can be done safely with assistance from your instructor. Assess
the size of a fire and make an effort to CONTAIN THE FIRE. Wet paper towels are very efficient for
smothering small fires. Use the fire extinguisher for moderately sized fires. If there is a large fire, close the
doors to the adjacent laboratories, evacuate the laboratory, close the entrance door to the laboratory and
LEAVE THE BUILDING.
The following information must be provided prior to participation in the Microbiology Laboratory.
Course _________________ Section ________________ Room_______
Time ___________________ Instructor ___________________________
I understand that participating in the microbiology laboratory exposes me to potential hazards. I have read and
understand the above safety rules and agree to abide by them and follow the directions of the instructor. I agree
to act responsibly and safely in the laboratory at all times. Furthermore, if I am pregnant or immunocompromised
or discover that I have become pregnant or immunocompromised, I will notify my instructor at my earliest
opportunity.
_______________________
Signature
__________________________
Print Name
_________
Date