SYLLABUS
MICROBIOLOGY – MCB 2010C (4 credit hours)
Summer 2009
Instructor: Kim Lorinda Tash
Office: Adjunct Faculty Office
Email: Ktash@mail.ucf.edu
Required Textbook:
Microbiology: An Introduction 9th edition by Tortora, Funke and Case (ISBN
0-8053-4791-7).
Required Lab Manual:
Microbiology Laboratory Theory and Application (Brief Edition). Leboffe and
Pierce, 2008 (ISBN 0-89582-705-0).
Additional Materials:
Each student will need a lab coat or acceptable substitute, latex gloves for lab
work, and a permanent marking pen (fine or extra fine point).
Prerequisite:
Microbiology is traditionally a course taught at the sophomore level and you
Should have had a minimal grade of C in either BSC 1005 and BSC 1005L or
BSC 1005C or BSC 1010C or BSC 2093C to take this course.
Course Description:
The course stresses the structure, nutrition, growth, control, metabolism, and
introductory genetics of bacteria. Characterization of representative groups of
bacteria, fungi, and viruses is included. Laboratory experience in techniques
and primary isolation will be provided.
Course Objectives:
Upon successful completion of this course the student will be able to:
1.
Describe the tremendous diversity, distribution, and growth requirements
for microorganisms.
2.
Safely handle and identify bacteria
3.
Describe the basic concepts of microbial structure, metabolism, genetics,
and mechanisms of microbial pathogenicity.
4.
Collect, interpret, analyze, and evaluate data.
5.
Critically analyze experiments.
6.
Define and solve problems.
7.
Describe some of the more common bacteria, fungi, and viruses which are
harmful to man.
8.
Recognize that many microorganisms are beneficial to man.
9.
Transform bacterial cells through the use of plasmids.
GRADING POLICY:
The final grade will be determined as follows.
Lecture Grade
4 Unit Exams worth 100 points each
(The lowest of the 4 grades will be dropped)
300 points
Final Exam
180 points
Lab Grade
(1 Lab Exams worth 100 points,
Lab Technique Grade worth 20 points, and
MMWR Report worth 50 points)
170 points
Quizes
Lab Quizzes (10 points each)
100 points
TOTAL POSSIBLE
750 points
90% - 100%
80% – 89%
70% – 79%
60% - 69%
Below 60%
A
B
C
D
F
Borderline grades (e.g. a final average of 79.8%) will be determined based on factors such as
attendance, punctuality, and class participation.
Students with Disabilities
Students with disabilities who qualify for academic accommodations must provide a letter from
the Office of Students with Disabilities (OSD) and discuss specific needs with the professor,
preferably during the first week of classes. The office of Students with Disabilities determines
accommodations based upon appropriate documentation of disabilities (West campus SSB 102,
ext 1523)
Lecture Grade:
Each Unit Exam will cover 2 - 4 chapters from the text and will consist of multiple choice
questions. The lowest of these 4 exams grades will be dropped.
Anyone missing an exam for jury duty, court-mandated appearance or observance of religious
holiday must contact the instructor in advance as outlined in VCC’s General Attendance Policy.
Anyone missing an exam for any other reason must present an excuse from an appropriate
authority (doctor, police, etc.) within 24 hours of the start of the exam. No “make-up”
examinations will be given without an acceptable documented excuse.
Lab Grade:
The first lab exam covers all of the exercises performed during the first half of the course and
consists of both a written and a practical component. The second lab exam (practical only)
covers those labs performed during the last half of the course and the media and procedures used
to determine the unknown. Lab practicals are particularly difficult and time-consuming to
prepare and make-ups for these will only be given at the discretion of the instructor, and only by
prior arrangement. Make every effort to attend scheduled practicals.
The lab technique grade is based on lab attendance (the first absence will not result in point
deductions, however, every absence thereafter will count 2 points against your technique grade),
preparation, and participation as well as the appropriate care of microscopes and other equipment
and the proper storage and disposal of microbial cultures. Mastery of basic microbiological
skills, such as aseptic technique, will also be taken into account.
The unknown determination will be performed during the last half of the semester. The
Unknown Defense is an oral exam in which the student presents his or her results to the
instructor and answers questions regarding the tests that were run. More information about the
procedure for unknowns will be distributed as the course progresses.
ATTENDANCE AND WITHDRAWAL POLICY :
Regular attendance is required in order to be successful in the course. Attendance will be taken
at each class meeting. Students who do not maintain regular attendance and who fall behind in
their work are subject to withdrawal by the instructor. A student may withdraw at any time
during the term in Atlas. Students will receive a W if withdrawn by July 24th 2009. After this
date, if a student withdraws or is withdrawn by the professor for excessive absences or other
reasons, the professor will assign a grade of WP (withdrawn passing) or WF (withdrawn failing),
based upon the student’s academic achievement in the class as of the last day of attendance. W
and WP will not be counted in the grade point average. WF will be calculated as an F in the
grade point average.
STUDENT RESPONSIBILITIES:
This is a college-level science course and, as such, will require academic self-discipline and good
study skills on your part. Regular attendance, good note-taking, and a willingness to "keep up"
and seek help when you need it will contribute to your success in this class.
Cell phones and other electronic devices must be placed in backpacks or purses with ringers
turned off before entering class. Talking on the phone, checking voicemail, playing video
games, and text messaging are not allowed during class time and may result in deductions from
your final grade or instructor–initiated withdrawal from the class.
MON
June 22
TUES
WED
THUR
June 23
June 24
Class: Chp 1, 14
June 25
June 30
July 1
Class: Exam 1
Chp 4
Lab: Quiz 2
July 2
July 6
Class: Chp 13
Lab: Quiz 3
July 7
July 9
July 13
Class: Chp 20
Lab: Quiz 5
July 14
July 20
Class: Chps 5, 6
Lab: Quiz 7
July 27
Class: Exam 4
Chp 15
Lab: Quiz 9
August 3
Class: Final Exam
Lab: Lab Practical
Turn in MMWR report
July 21
July 8
Class: Exam 2
Chp 7
Lab: Quiz 4
July 15
Class: Exam 3
Chp 5
Start MMWR report
Lab: Quiz 6
July 22
Class: Chps 6, 8
Lab: Quiz 8
July 29
Class: Chps 16, 17
Lab: Quiz 10
June 29
Class: Chp 2
Lab: Quiz 1
Cl
July 28
July 16
July 23
July 30
August 4
MICROBIOLOGY CLASS SCHEDULE
MICROBIOLOGY (MCB 2010C) LABORATORY EXERCISES
Valencia Community College
Summer 2009
Week 1. Lab Introduction (June 24th, Wednesday)
- Safety and laboratory Guidelines: students read pp. 1-6
- “Scavenger hunt” (introduce students to location of safety equipment)
- Exercise 2-1 Ubiquity of Microbes
- Exercise 3-1 Introduction to the Light Microscope
- learn parts, use and care of the microscope; crossed thread and letter “e” prepared
slides,
- Exercise 3-3 Examination of Eukaryotic Microbes
Week 2. Culturing Bacteria and Using the Microscope (June 29th, Monday)
- Students read Exercise 1-2 about Nutrient Agar and Nutrient Broth Preparation, page 19, and
pp. 55- 62 about Colony Morphology
- Exercise 1-3 Common Aseptic Transfers and Inoculation Methods
- Exercise 1-4 Streak Plate Methods of Isolation
- Exercise 3-1 Introduction to the Light Microscope (continued)
- test students on ability to clean the microscope’s oil immersion lens
- Exercise 3-3 Examination of Eukaryotic Microbes (cont.)
- Exercise 3-10 Wet Mount Preparations
Week 2. Staining I (July 1, Wednesday)
- Students read pp. 65 and 69 about Growth Patterns on Slants and Broth
- Students read pp. 145-148
- Exercise 3-4 Simple Stains
- Exercise 3-5 Negative stains
Week 3. Staining II (July 6, Monday)
- Exercise 3-6 Gram Stain
- Exercise 3-7 Acid-Fast Stain (prepared slides for observation)
- Exercise 3-9 Endospore Stain (prepared slides for observation)
Week 3 Selective and Differential Media (July 8, Wednesday)
- Students read pp. 73, 197 and 225
- Exercise 4-1 Mannitol Salt Agar
- Exercise 4-2 Phenylethyl Alcohol Agar
- Exercise 4-3 Columbia CNA with 5% Sheep Blood Agar
- Exercise 4-4 Eosin Methylene Blue Agar
- Exercise 4-5 Hektoen Enteric Agar
- Exercise 4-6 MacConkey Agar
Week 4. Physical Growth Factors for Bacterial Growth (July 13, Monday)
- Students read pp. 101 and 115
- Exercise 2-6 Fluid Thioglycollate Medium
- Exercise 2-8 The Effect of Temperature on Microbial Growth
- Exercise 2-9 The Effect of pH on Microbial Growth
Bacterial Nutrition
-Exercises 5-11; 5-13; 5-16
Week 4. Introduction to Biochemical Test Media (July 15, Wednesday)
-Students read 497-498
- Exercises 5-4; 5-5; 5-6; 5-9; 5-10; 5-18; 5-19; 5-20; 5-21
Week 5. Culture and Sensitivity (C&S) (July 20, Monday)
- Students read page 455
- Exercises 5-2; 5-3; 5-7; 5-8; 5-12; 5-14; 5-15; 5-17
- Exercise 9-1 Identification of Enterobacteriaceae
- Exercise 7-2 Antibiotic Susceptibility Test (Kirby-Bauer Method)
Week 5. C&S: Unknown Identification Lab and Antibiotic Selection (July 22, Wednesday)
- Students read pp. 35, 481-484 (API 20 E) and pp. 489-493(Enterotube II)
- Exercise 9-1 Identification of Enterobacteriaceae (analysis)
- Exercise 7-2 Antibiotic Susceptibility Test (Kirby-Bauer Method) (analysis)
Week 6. DNA Lab (July 27, Monday)
- Exercises 2-11 and 8-2 The Lethal Effect of Ultraviolet Light on Microbial Growth
- instructors read handout to see how this lab will performed differently from the book
- Exercise 8-3 Bacterial Transformation: the pGLO System
Week 6. Human Microbiology (July 29, Wednesday)
- Students read pages 35 and 365
- Exercise 6-4 Differential Blood Cell Count
- Exercise 7-1 Snyder Test
- ELISA Test
Epidemiology Simulation Lab
- Exercise 7-4 Epidemic Simulation (Influenza party )
Review
Week 7. FINAL LAB (August 3, Monday)