Enzyme Lab
Presentation
We Chose to do Our
Experiment on How
Effective the Enzyme
Sucrase is at Producing
Glucose When Kept at
Different Temperatures
• Sucrase is an enzyme which helps in the digestion of
food. Digestive enzymes are in charge of breaking
down food, allowing for the proper absorption of
nutrients.
• Sucrase enzymes are secreted by the tips of the villi of
the epithelium
• Sucrase fits perfectly into the bond between the
glucose and fructose molecules that make up sucrose
• Sucrase works by bringing about a chemical
reaction in sucrose that divides it into glucose and
fructose
• Sucrase enzymes occur naturally in the human
body. However, eating poorly, natural aging, taking
certain medications, and pre-existing conditions of
intestinal problems may lead to a reduction in the
amount of sucrase your body produces.
1. First we got 16 test tubes and put them in a test tube rack
2.We put 3 mL of sucrose solution into each test tube
3.We put 0.5 grams of yeast into 12 of the test tubes. The other 4 test
tubes will serve as the control so will not contain any yeast
4. Then we separated the test tubes into four groups. Each group had 3
test tubes containing sucrose solution and yeast and 1 test tube
containing just sucrose solution
5.We left the first group of test tubes on our desk at room temperature
(about 20°C). We put the second group into an incubator ( at 37°C).
We put the third group in an ice bath ( about -1°C). And we put the
fourth group in a boiling water bath ( about 100°C)
6.Then we left the test tubes for 15min.
7.After 15min. was up we brought all the test tubes back to our desk
and tested them all for glucose with glucose test strips.
8.Finally we recorded our results
• Independent Variable (is the one that is changed) =
Temperature: Room Temperature (20°C), Incubator
(37°C), Ice Bath (1°C), and Boiling Water (100°C)
• Dependent Variable( the variable that is observed
and responds to the change made to the independent
variable) = Amount of glucose produced measured in
milligrams per decilitres
• Controlled Variables ( the variables that are kept
constant) = Amount of Sucrose solution in each test
tube ( 3 mL), Type of sucrose/sugar, amount of yeast
in un-controlled test tubes (0.5g), type of yeast, and
amount of time the test tubes are left in different
temperature conditions (15 minutes)
• We set up a control group by simply not
adding any yeast to four of the test tubes.
• We put one of these controlled test tubes in
each of the 4 different temperature
conditions.
• We put one in room temperature, one in the
incubator, one in the ice bath and one in the
boiling water bath.
Room Temperature
Incubator
Ice Bath
Boiling Water
Test Tube
Glucose
Formation @
Room Temp.
(20°C)
Glucose
Formation in
Incubator
(37°C)
Glucose
Formation in
Ice Bathe (-1°C)
Glucose
Formation in
Boiling Water
(100°C)
#1
150mg/dL
250mg/dL
800mg/dL
1200mg/dL
#2
100mg/dL
300mg/dL
850mg/dL
1000mg/dL
#3
200mg/dL
350mg/dL
900mg/dL
1250mg/dL
Control
negative
negative
negative
negative
1400
1200
1000
Test tube # 1
800
Test Tube #2
600
Test Tube #3
Control
400
200
0
Room Temp.
Incubator
Ice Bath
Boiling Water
•
Our lab experiment outcome was fairly inaccurate
• Our results were supposed to favour the tests in the incubator
however; they actually favoured the boiling water.
• These results are incorrect because yeast; like enzymes is sensitive
to temperature. The boiling water should have killed the yeast
and denatured the sucrase enzymes; therefore there should have
been very little if any glucose formation.
• The Ice bath also should have slowed down the enzyme’s activity
and not of produced as much glucose as it did.
• The incubator is the most accurate representation of the
temperature in the human body (37°C) therefore; the sucrase in
the yeast should have produced more glucose in that test but for
some reason that we can’t understand it did not.
The End!
Hope you Have a
Very Merry
Christmas and a
Happy New Year 
Christmas Carol
Sucrase The Digestive Enzyme (to the tune of Rudolf the Red-Nosed
Reindeer)
Sucrase, the digestive enzyme
has a very active site.
And if you find its substrate,
you would even say it fits (like a puzzle)
All of the other enzymes
used to bind and make new things
they never let poor Sucrase
join in any enzyme games
Then one chemical reaction
Sucrose came to say:
"Sucrase with your active site,”
won't you bind with me tonight?"
Then all the enzymes joined in
As glucose and fructose were formed
Sucrase the digestive enzyme
you'll produce monosaccharide’s