LABORATORY GUIDE NATURAL SCIENCE AND ENVIRONMENTAL EDUCATION DEPARTMENT GIMNASIO LOS PINOS Elaboro: Jefe Ciencias Aprobó: Dir SGC Fecha de emisión: 9-Jun-10 Código: GCU-LAB-03 Versión.1 Página 1 de 3 LABORATORY GUIDE No. 2.2 PRACTICE NAME TEACHER: ____________________________________________ GRADE: _____ DATE: ____________ GROUP NAMES: OBJETIVE: Recognize the main organ of the excretory system, its parts and functions. THEORETICAL FRAMEWORK: Basic Kidney Anatomy There are three primary components to a kidney: 1. Renal Capsule: A smooth semitransparent membrane that adheres tightly to the outer surface of the kidney. 2. Renal Cortex: The region of the kidney just below the capsule. In a fresh kidney the colour of the cortex will be reddish brown. 3. Renal Medulla: The region deeper into the kidney, beneath the cortex layer. In a fresh kidney it is more red in coIour than the cortex. It is segregated into triangular and columnar regions. The triangular regions are the renal pyramids, which should be striated (or striped) in appearance due to the collecting ducts running through them. The columnar regions between the pyramids are the renal columns. These renal columns are where the interlobar arteries are located. LABORATORY GUIDE NATURAL SCIENCE AND ENVIRONMENTAL EDUCATION DEPARTMENT GIMNASIO LOS PINOS Elaboro: Jefe Ciencias Aprobó: Dir SGC Fecha de emisión: 9-Jun-10 Código: GCU-LAB-03 Versión.1 Página 2 de 3 MATERIALS: Lamb FROZEN Dissection kit (knife – dissection board) Camera 1/8 cardboard (Flags - labels) Red plastic bag Pins kidney Towel Goggles Gloves Liquid soap Lab coat Surgical face mask PROCESS: Activity 1: 1. Follow the instructions in this dissection guide to identify all the structures in the kidney. 2. Collect dissecting tools, a ruler and a tray for your lab group. Obtain a fresh lamb kidney, it must be frozen. 3. Observe carefully the kidney, identify what you are seeing. 4. Take the kidney and lay it flat on your dissection board. 5. Continue with the following specific steps. 1. Observe in detail the kidney. 2. Identify the following parts. 3.Handle sharp instruments with caution. Always point 4. Call to your teacher and cut the kidney in half longitudinally them and cut away from yourself and anyone else who is using the knife or with short repeated strokes of the scalpel. DO nearby. IT WHEN YOUR TEACHER IS PRESENT, WITH YOU 5. Examine the interior structure of the kidney. Identify the cortex, medulla, pelvis, ureter, nephrons and any blood vessels present (renal vein / renal artery). 6. Complete a biological drawing of the interior of one half of the kidney, or take some pictures with your camera. 7.Use pins and the labels provided to make small flags to LABORATORY GUIDE NATURAL SCIENCE AND ENVIRONMENTAL EDUCATION DEPARTMENT GIMNASIO LOS PINOS Elaboro: Jefe Ciencias Aprobó: Dir SGC Fecha de emisión: 9-Jun-10 Código: GCU-LAB-03 Versión.1 Página 3 de 3 indicate the following structures: - Renal vein - cortex - medulla - renal artery - ureter - Nephrons 8. 9. Dispose of the kidney in the waste bag provided. Wash all dissecting equipment and return. Wash your hand thoroughly with warm soap and water. COLLATED DATA: Questions 1. Measure the kidney: Length: _cm Width: _cm 2. 2. What animal has this kidney come from? How does the size of this animal’s kidney compare to a human’s kidney (Our body)? _________________________________________________________________________________________________ ________________________________________________________________________________________________ ________________________________________________________________________________________________ ________________________________________________________________________________________________ 3. 3. It should be possible to identify three tubes entering or leaving the kidney. What are these called? (3 marks) _________________________________________________________________________________________________ _________________________________________________________________________________________________ _______________________________________________________________________________ 4. UTake a photo. BIBLIOGRAPHY. http://www.scienceteacherprogram.org/pdf/JBader11KidneyDissectionGuide.pdf http://www.abpischools.org.uk/page/modules/homeostasis_kidneys/kidneys2.cfm?coSiteNavigation_allTopic=1