LIST SLIDES FOR BIOLOGY 7 Lab 2 – Eubacteria and Cyanobacteria Prepared bacterial slides -bacteria – 3 types -bacillus – Mycobacterium tuberculosis – gram-ve or gram +ve -Salmonella typhus (gram –ve) -E.coli (gram –ve) -coccus – Neisseria gonnorhea (gram –ve) -Staphlyococcus aureus (gram +ve) & Micrococcus luteus (gram +ve) -spirillum – Treponema pallidum (syphilis) or Spirillum voluntans -colonial – Corynebacterium diptheriae (gram +ve) - Cyanobacterial slides -Nostoc -Anabaena -Oscillatoria -Rivularia Cyanobacteria wet mounts – Anabaena, Spirulina, Oscillatoria, Fisherella Lab 3 – Protists Lab Study A – Euglenozoans -prepared slides -Trypanosoma cruzi (kinetoplastid) -Typanosoma lewisi -Trypanosoma gambiense -Leishmania donovani (kinetoplastid) -Euglenoid (euglenid) -wet mounts – Euglena Lab Study B – Alveolates -prepared slides - Toxoplasma gondii (apicomplexan) -Plasmodium (apicomplexan) -Paramecium conjugation (ciliate) -Paramecium fission -Balantidium - tropozoites (ciliate) -Stentor (ciliate) -Vorticella (ciliate) -Ceratium (dinoflagellate) Lab Study C – Stramenophiles -prepared slides – diatoms Lab Study D – Rhizaria -prepared slides – Radiolaria -Globigerina (foram) -foraminiferans Lab Study E – Amoebozoans -prepared slides – Amoeba (gymnamoeba) -Amoeba proteus -physarum polycephalum (plasmodial slime mold) -wet mounts – Amoeba proteus, Physarum polycephalum (plasmodial), Lab Study F – Green Algae -prepared slides – Spirogyra conjugation (demo slide) -Volvox -Chlamydomonas -wet mounts – Ulva, Volvox, Spirogyra, Chlamydomonas Lab Study G – Diplomonads & Parabasilids – prepared slides: Trichomonas vaginalis (parabasalid) -Giardia lambia (diplomonad) Lab 4 – Fungus Lab Study A – Zygomycota -prepared slides – Rhizopus Stolonifer mycelium & zygosporangia -Mucor sporangia and zygotes -3 mold types Lab Study B – Ascomycota (Sac Fungus & Yeasts) -prepared slides -Penicillium conidiophores -Penicillium mycelium -Aspergillus conidiophores -Pezizia ascospores -mold types – Rhizopus, Aspergillus, Penicillium Lab Study C – Basidiomycota (Club Fungus) -prepared slides – Coprinus pileus and gills (cs and ls) Lab Study D - Lichens -prepared slides: Physcia lichen Demo slides: budding yeast–Saccharomyces cerevisiae -Candida albicans yeast -Aspergillus cleistothecia -Morel gill wet mount -Mushroom gill wet mount Lab 5 – Non –vascular plants (Mosses & Liverworts) and Seedless vascular plants (Ferns) Lab Study A: Bryophytes (Moss) -moss life cycle with archegonia and antheridia -Mnium archegonium -Mnium antheridium -moss protonema Lab Study B: Hepatophytes (Liverworts) – Marchantia sp. -liverwort sporocyte -liverwort life cycle with archegonium and antheridium Demo slides-liverwort antheridium -liverwort archegonium -liverwort gemmae cup Lab Study C: Seedless Vascular Plants (Ferns, Clubmosses and Quillworts) -Lycopodium strobilus with sporangia (quillwort) – cross-section and longitudinal section -Selaginella strobilus – cross section and longitudinal section Lab Study D: Ferns, Horsetails and Whiskferns -fern life cycle -fern sporangium with indusium -Cyrtomium (holly fern) sorus with indusium -fern prothallium with archegonia -Psilotum (whisk fern) sporangia longitudinal section (l.s.) -Equisetum (horsetail fern) sporangia – cross section and longitudinal section -Equisetum spores Demo slides-fern archegonium Lab 6 – Seed plants - Gymnosperms Prepared slides – Pine archegonia/ovule l.s -Pine pollen grains -Pine ovulate cone l.s. -Pine staminate cone l.s -Male pine with developing staminate cones -Pine leaf in cross section -Pine ovule with immature embryo -Pine embryo with elongating suspensor -Pine young leaf with needles -Pine young stem and 1 yr stem (secondary growth) Lab 7 – Angiosperms Lab Study A: Flower & Fruit morphology Prepared slides: –floral bud -pollen tube Demo slides – germinating pollen -Lily ovary megaspore mother cell -double fertilization Demo dissection scopes -Mum ray flower with carpel and stamens -Mum disc flower with carpel and stamens -perfect flower – rose anthers -perfect flower - lily carpels -perfect flower – lily anthers & carpel -perfect flower – lily ovary with ovules Lab Study C: Angiosperm Life Cycle Prepared slides – lily anthers – 1st division and tetrads -monocot pollen – typical pollen grains -capsella mature embryo -lily ovary embryo sac -lily floral bud -zea kernel and embryo Demo slides – germinating pollen grains -double fertilization -megaspore mother cell Lab 8 – Plant Anatomy Prepared slides – monocot leaf section – Zea mays -monocot stem section – Zea mays -monocot root section – Zea mays -monocot root tip – Zea mays -dicot leaf section – Syringa -dicot leaf section – shade and sun -dicot leaf bud with abcission zone -dicot stem section -dicot root section – Ranunculus -dicot root tip - Ranunculus -apical meristem – Coleus stem tip -dicot stems – annual and mature secondary growth –Quercus rubra (Oak) -dicot stems – woody growth 1,2,3 yrs – Tilia -secondary growth – pine stem -plant tissues: pine needle (c. s) -psilotum shoot cross section -equisetum shoot cross section Demo slides- Pine stem - xylem and tracheids & phloem (green) and sieve cells -secondary xylem from Oak wood – xylem rays -Rubinia wood – tracheids and vessel elements (c.s) -Quercus rubra – earlywood, latewood -section through a woody stem (Tilia) – growth rings -Zea mays – monocot leaf vascular bundle -Ranunculus - dicot root - endodermis -Medicago (alfalfa) – dicot stem – vascular cambium -dicot stem – fasicular cambium -dicot leaf Syringa (lilac) leaf -air, water and soil roots -primary and secondary roots -root nodules Lab 9 – Animal Diversity I Sponges & Cnidarians Lab Study A – Sponges Prepared slides – Scypha (c.s) -commercial sponge fibers - Leucoselenia -Grantia choanocytes -Grantia spicules Demo slides – sponge gemmules -scypha/Grantia – incurrent canals Lab 9 cont…. – Animal Diversity I Lab Study B – Cnidarians Prepared slides – Hydra whole mount -hydra with bud (l.s.) -hydra (l.s.) -hydra cross section -Aurelia medusoid form -Obelia young medusa -Obelia hydroids Demo slides – Metridium (c.s) Lab 10: Worms Lab Study A – Platyhelminthes Prepared slides – planaria whole mount -planaria digestive tract -taenia pisiformis (tapeworm) -dwarf tapeworm – hymenolepsis nana -liver fluke (Clonorchis sinensis) -giant liver fluke (Fasciola gigantica) -Fasciola hepatica – miracidium and cercaria stages -leech Demo slides – tapeworm scolex -tapeworm proglottids -schistosoma male vs. femal Lab Study B – Nematodes Prepared slides – trichenella spiralis (roundworm) -ascaris (c.s) – male and female -pin worm (enterobius vernicularis) Demo slides - hook worm (Necator americanus) Lab Study C – Annelids Prepared slides – earthworm (c.s) – anterior to clitellum -earthworm (c.s.) posterior to clitellum -earthworm nephridium -earthworm – three sections Demo slides – Nereis sandworm – parapodia & setae -Lumbricus sections