Homework: Review your lab notebook and find lab procedures that you can apply to this project. Draft 1 of lab procedure & materials in your lab notebook.
 Welcome back
 Final project overview and schedule
– Clarification & questions
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Restriction analysis challenge
Samples – gene cloning

Restriction Analysis:
To determine the presence of the amylase gene in a plasmid

 Individual project – no partners or teams
 Replaces the final exam
 25% of your course grade
– Same as final exam

 Extension of your project on rDNA
 Use of biotechnology procedures already practiced in the lab.
 Demonstrate that you can understand and summarize technical information that provides background to your laboratory work.

 Literature Research to provide background to the laboratory work
 Designing an experiment
– Writing a lab experiment with procedures that you will follow
 Conducting the experiment
 Analyzing the results and making conclusions

 Steps in Cloning a Gene

 1/3: Explore the sources on the Internet
– Credible sources
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Understandable to you as a high school student
Provides useful information for the topic
 Laboratory Work Defined: due 1/4
Hypothesis, materials & equipment & lab procedures must be submitted prior to lab work
 This will be peer reviewed on Friday, 1/4
 Revised lab procedure due Monday, 1/7

 Laboratory Work - 2 – 3 days (1/7?, 1/8;
1/9; 1/10?)
 Background research paper due 1/9/13
 Completed Laboratory Report due: 1/11/13
 Final Exam Day
– Short Presentations
– Reflection of your learning

Read the project description
Write questions & hand-in :
 Literature Research to provide background to the laboratory work
 Designing an experiment
 Conducting the experiment
 Analyzing the results and making conclusions

 Restriction Analysis Challenge 2013.ppt

 Components
– Amylase enzymes for function and possible size
– Process of incorporating desired genes into plasmids
& then bacteria
– Detection of successful take-up of desired DNA fragment by plasmids & then into bacteria
 Requires documented notes from each source
 Starting point: gene cloning description on assignment paper

4 3 2 1
Content and Focus
Organization
Style
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Exceptionally clear, focused, interesting thesis.
Strong, rich supporting details and examples that prove thesis.
A meaningful conclusion explaining the importance of the research and how it can be used.
Strong introduction and conclusion.
Consistent and coherent logical progression.
Uses clear and skillful transitions.
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Clear thesis which maintains a consistent focus from beginning to end.
Specific supporting details are present.
A clear conclusion as to why the research is important.
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Clear introduction and conclusion.
Illustrates some consistency and shows some logical progression.
Uses clear transitions.
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Written in formal language
(avoids slang completely).
Elaborate and colorful language.
Consistently strong and varied sentence structure.
Direct quotes support student’s ideas.
Paper written in student’s own words.
Majority of paper written in formal language.
Language appropriate to topic.
Words convey intended message.
Direct quotes support student’s ideas.
Majority of paper written in student’s own words.
Contains thesis but with inconsistent focus.
Generalized supporting details that prove thesis.
Conclusion tends to summarize research.
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Thesis statement lacks clarity and focus.
Inadequate or missing supporting details.
Missing a summarizing conclusion
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Introduction and conclusion is present but not clear
Show some attempt of consistency and order.
Paper shows attempt of transitions between paragraphs.
Some use of formal language recognized; informal language is dominant.
Most language is appropriate to topic.
Able to get vague idea of message.
Some parts of paper written in student’s own words.
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Unable to clearly identify introduction and conclusion.
Lack of consistency and order.
Paper shows little or no attempt of transitions between paragraphs.
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Paper frequently uses informal language.
Language is not appropriate to topic.
Message is unclear.
Majority of paper is plagiarized.

Sources/Format
Conventions
Follows MLA guidelines:
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Uses 5 or more cited sources.
Sources meet the guidelines for types of sources.
All parenthetical documentation is MLA correct
(author’s name, pg. #).
Works Cited page is MLA correct.
All researched info is documented.
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Follows MLA Guidelines with few exceptions:
3-4 cited sources used.
Sources meet the guidelines for types of sources.
Few errors noted in parenthetical documentation
Majority of Works
Cited page is MLA correct.
Most research info is
Documented.
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Inconsistent use of
MLA style guidelines.
Less than 3 cited sources used.
Majority of parenthetical documentation done incorrectly.
Random MLA documentation.
Rarely documents sources.
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Superior editing. Rarely makes errors in the following areas:
Spelling and mechanics.
Correct usage and grammar.
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Careful editing. Makes few errors in the following areas:
Spelling and mechanics
Correct usage and grammar
Some evidence of editing:
 Extensive spelling and grammatical errors.
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Fails to follow MLA style
Guidelines.
Less than 3 cited sources used little or no parenthetical documentation.
Works Cited page is not understandable.
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Poor editing:
Spelling and grammatical errors make it difficult to read paper.

 By Friday , typed for peer review – use ID number (no name)
– Purpose
– Hypothesis: prediction of what DNA fragments that you are looking for
– Materials
– Equipment list
– Detailed procedure
– Identified controls
– Data table

 Blind review – no names on papers
 Purpose: to optimize the lab procedures to ensure useable results
– Provide feedback to another student to improve her/his lab procedure
– Gain perspective on good/poor lab procedures to revise your lab procedure

 Shared equipment
– Water bath, Gel electrophoresis, etc.
 Waiting time: come prepared to work
– Lab report
– Presentation
 Analysis process and conclusions
– How did you use your results and research in your analysis
 What learned in doing this final project

 How is DNA cloned.ppt