Lentikats Biotechnology
in Organic Acid Production
Content
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What is the Lentikats biocatalyst?
How is the Lentikats biocatalyst produced?
Benefits of the Lentikats biotechnology
Lentikats biocatalyst and its usage in organic acid production
Partners
Guarantees
Contacts
What is the Lentikats Biocatalyst?
Unique shape of the matrix and original immobilization procedure enables to
encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a solid
porous carrier from polyvinylalkohol (PVA). The advantages of PVA cells are:
• Excellent physical and mechanical characteristics that result in a long-term
mechanical stability
• Hardly biodegradable
• No toxic effects
Lentikats biocatalysts enable easy separation of enzymes or microorganisms from
the bulk media and therefore can be used repeatedly.
Biotechnologies are changing
the world…
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats
biocatalyst results in a substantial reduction of diffusion limitations associated
with the transfer of substrate into the matrix and the product out of the
matrix.
Why polyvinylalcohol?
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It is an inexpensive material
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It is a non-toxic material
Encapsulation takes place under optimal, cell-sparing conditions
It allows a production of hydrogel with excellent mechanical properties
It does not have any side effects on fermentation
It offers high capacity for the cell loading
The process is stable and easy to control
Biotechnologies are changing
the world…
How is the Lentikats Biocatalyst
produced?
What can be immobilized?
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Free cells
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Dead cells
Living non-growing cells
Living growing cells
Free or immobilized enzymes
The size of Lentikats biocatalyst pores varies depending on the size and diameter of
the biocatalyst due to:
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type of PVA additives (size of macromolecules)
concentration of additives
biomass concentration
Laboratory Units for the Encapsulation
• Lab scale units with a low production capacity of Lentikats
biocatalysts (for research purposes) are also available
Lentikats Biocatalyst – production line
What are the benefits of LB?
Biotechnologies
that are environmentally friendly …
…and also…
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Lower biochemical reaction time
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Reuse of the biocatalyst (sieve
separation)
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Reduction of the risk of
contamination during fermentation
processes
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High stability of production
processes
In comparison with conventional
technologies much higher yield (i.e.
5 – 15% higher)
Lentikats Organic Acid Production
Solutions
Lentikats biotechnology is used in the following processes:
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lactic acid production from glucose, sucrose and lactose
(immobilized bacteria Bacillus coagulans)
gluconic acid production from sucrose and glucose
(immobilized filamentous fungi Aspergillus niger)
itaconic acid production from sucrose and glucose
(immobilized filamentous fungi Aspergillus terreus).
Lentikats Biotechnology
LENTIKATS BIOTECHNOLOGY FOR LACTIC ACID PRODUCTION
Producer: bacteria Bacillus coagulans.
Substrates: glucose, sucrose, lactose.
Production:
• batch, fed batch and continuous lactic fermentation is stable for all C-substrates.
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saccharose fermentation solutions with 60 g/l of saccharose concentrate (The total fermentation
process was decresed from 48 hours in „the first conversion“ to 12 hours in „the tenth
conversion“ with a lactic acid yield of 91 to 93 %.)
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glucose fermentation solution with 60 g/l glucose concentrate (After 14 hours fermentation
broths consist of 55.2 g /l of lactic acid and 1.2 g /l of glucose in the tenth conversion.)
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fermentation productivity is increased from 13.5 to 17.0 (g LA.dm-3.h-1).
Lentikats Biotechnology
LENTIKATS BIOTECHNOLOGY FOR ORGANIC ACIDS PRODUCTION
Producer:
1) filamentous fungi Aspergillus terreus for itaconic acid production
2) filamentous fungi Aspergillus niger for gluconic acid production
Substrates: sucrose, glucose
Production:
• extensive production of itaconic acid is not stable yet
• first results indicate that if the initial concentration of sucrose is 50 g/l then
the concentration of itaconic acid is 18.5 g/l in the batch mode process
What does Lentikats Biocatalyst bring?
Optically clear Llactic acid (99%)
manufacturing
Fermentation
processes
regulation
Biomass
regeneration
costs decrease
Robustness
High
specific
activity
High yield of
lactic acid
(about 20 %)
Operational costs decrease
Delivering on Lentikats Biotechnology Key
Benefits
Significant
Notable cost
reduction
improvement of
operating economic
results
Low risk of
contamination
Decreased
fermentation volumes
Increased
operational costs
High Productivity
Short fermentation time (10 hours)
High concentration of
Lentikats Biocatalysts in fermentors
Decreased sales
revenue
High yields
About 91-93 %
Reusable
Lentikats Biocatalysts
Our partners in the field of industrial
applications
geniaLab®, BioTechnologie-Produkte und
Dienstleistungen Gmbh
Büchnerstraße 7, 38118 Braunschweig, Germany
www.genialab.com
Lentikats Biotechnology – guarantee of your
investments and the investment return
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You can test our biocatalyst easily by yourself with your substrate in your own plant
or plant of your choice to see the results.
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We offer you our assistance and full support.
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We offer a long-term cooperation to the engineering companies specialising in the
design of distilleries and are interested in the implementation of technologies
requiring minimal investment at high profitability.
Biotechnologies are changing
the world…
Lentikats Biotechnology – Investment
and Investment Return Guarantees
We guarantee for the following:
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delivery of production facilities within 24 months
delivery of output parameters
operational stability of Lentikats biocatalyst and their activity and
productivity
purchase price stability within 36 months.
Our partners in the field of research and
development
Institute of Microbiology, Academy of Science
of the Czech Republic,
www.biomed.cas.cz
Vídeňská 1083, 142 20 Praha 4, Krč
STU Bratislava, Faculty of chemical and food
technology, Institute of biotechnology and food technology,
Radlinského 9, 81237 Bratislava, SK
ICT Prague, Department of Fermentation Chemistry
and bioengineering, Technická 5, 166 28 Praha 6
University of Alberta, Edmonton Alberta, T6G 2J9
Canada
www.stuba.sk
www.vscht.cz
www.ualberta.ca
Lentikats Biotechnology Applications
Lentikats Biocatalysts and applied Lentikats Biotechnology also delivers
additional value and benefits in the following sectors:
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waste and industrial water treatment,
distillery industry – bioethanol production,
pharmaceutics – production of robust biocatalysts,
food industry – whey processing, production of special
sugars carbohydrates and syrups
Biotechnologies
are changing the world…
Contacts
Company Headquarters:
Laboratory:
LentiKat‘s a.s.
Office Building ARGO
Evropska 846/176a
160 00 Prague 6
Czech Republic
INBIT LentiKat‘s a.s.
Kamenice 34
625 00 Brno
Czech Republic
Tel.:
Fax:
E-mail:
+ 420 255 710 680
+ 420 255 710 699
info@lentikats.eu
Production plant:
LentiKat‘s a.s.
Pod Vinicí 83
471 27 Stráž pod Ralskem
Czech Republic
Tel.:
Fax:
E-mail:
+ 420 487 851 393
+ 420 487 851 399
info@lentikats.eu
Tel.:
Tel.:
+ 420 511 205 288
+ 420 511 205 287