Phylum Platyhelminthes Goals for today • Learn to recognized the Phylum Platyhelminthes from other animals • Learn the main ‘diagnostic’ characteristics • Learn about some free-living and parasitic species biology Phylum Platyhelminthes: flatworms These are acoelomate metazoans Parazoa Lophotrochozoa Protostomia Radiata Bilateria Eumetazoa Deuterostomia Ecdysozoa Annelida Mollusca Lophophorata Rotifera Platyhelminthes – Bilateral symmetry – Third germ layer-mesoderm (triploblastic animals) – Excretory system made up of specialized flame cells and tubules for removal of nitrogenous waste – Highly organized nervous system and sense organs in the anterior part of the body (cephalization) Cnidaria and Ctenophora More organized than radiate animals in that: Porifera Lophotrochozoa Phylum Platyhelminthes: Classification Classes: Turbellaria: • Ciliated epidermis • Paraphyletic group • Mostly free-living Cestoda: • Syncytial tegument without cilia • Long ribbon like body shape Trematoda: • Body divided in proglotids • Digenetic flukes (having two stages of multiplication, one sexual in the mature forms, the • Organs to attach host in the oral part of the body: suckers, other asexual in the larval stages) hooks • Syncytial (many nuclei within a single cell) • No digestive system tegument without cilia • Parasitic in digestive tract of • Leaf-like to cylindrical shape. all classes of vertebrates • Organs to attach host in the oral and ventral part of the body: suckers, NO hooks • Parasitic in all classes of vertebrates Phylum Platyhelminthes: Your Tasks Phylum: Platyhelminthes – Class Turbellaria • Genus: Dugesia Planarias are freshwater usually under stones or submerged leaves or sticks. But other members of the class are marine Phylum Platyhelminthes: Dugesia 1. Take a slide from your box with a planaria: identify the eyespot, aurículas, intestino, faringe, y boca. intestino boca What is the function of the eyespot and the auricles? ____________ Check your book for answers! Phylum Platyhelminthes: Dugesia As in cnidarians the digestive tract of turbellarians is a gastrovascular cavity, the branches of which fill most of the body. They don’t have an anus so undigested food is ejected through the mouth! Yikes Phylum Platyhelminthes: Dugesia Reproduction: these animals are monoecious (hermaphrodites) they can reproduce sexually and asexually by transverse fission Asexual reproduction, the planarian detaches its tail end and each half regrows the lost parts by regeneration, allowing neoblasts (adult stem cells) to divide and differentiate. sexual reproduction Planarians exchange sperm. Eggs develop inside the body and are shed in capsules. Weeks later, the eggs hatch and grow into adults. Sex is desirable because it enhances the survival of the species by maintaining the level of genetic diversity. Phylum Platyhelminthes: Dugesia Excretion and osmorregulation: the excretory system consist of canals and protonephridia called flamed cells. Phylum Platyhelminthes: Dugesia 2. Take a slide from your box with a planaria cross section cuts: identify intestino, diverticulos intestinal, lumen de la faringe, musculos dorsoventrales. Planaria – cross section circular muscle rhabdites longitudinal muscle epidermis intestine mesenchyme pharyngeal pouch lumen of pharynx pharynx Phylum Platyhelminthes: Dugesia 3. Observe live planarias. Take a look to their locomotion. How does it use the head and auricles? Does it ever move backwards? Phylum Platyhelminthes: Dugesia 4. Observe live planarias. Perform with your instructor the stimuli experiments described in page 136. • Response to touch • Response to food • Response to directional illumination Write your answers this is part of the Planaria report Phylum Platyhelminthes: Dugesia 5. Regeneration : Some planarians exhibit an extraordinary ability to regenerate lost body parts. A planarian split lengthwise or crosswise will regenerate into two separate individuals When divided in two it will first regrow the head. Body segment differ in capacity to regenerate, because the amount of neoblasts differs in the area around the cut. Phylum Platyhelminthes: Your Tasks Phylum: Platyhelminthes – Class Trematoda • Genus: Clonorchis sinensis Clonorchis lives in the human bile duct (bilis) where it feeds on bile and lacerated cells from the inflamed bile duct very common in Asia Phylum Platyhelminthes: Clonorchis 1. Observe slide of Clonorchis sinensis: identificar ventosa oral, boca, faringe, intestino, ventosa ventral, glandula de yema, poro genital, vesicula semina, ovario, receptabulo seminal, testículos, vejiga, poro excretor Phylum Platyhelminthes: Your Tasks Phylum: Platyhelminthes – Class Trematoda • Genus: Schistosoma mansoni Schistosoma are blood flukes of humans that affect ~ 200 million people in Asia, Africa, the Caribbean (including Puerto Rico!) and South America. Disease: Schistosomiasis Phylum Platyhelminthes: Schistosoma 1. Ciclo de vida: Observe slides of Schistosoma mansoni adults, eggs, and cercariae eggs adults miracidum cercariae You need to understand the life cycle Phylum Platyhelminthes: Your Tasks Phylum: Platyhelminthes – Class Cestoda • Genus: Taenia pisiformis- dog tapeworm Extreme parasites: No digestive system. Tegument specialized epidermis that absorbs nutrients and rejects toxins and digestive enzymes. Live is all about maximize reproduction. In fact tapeworms are egg factories, some species can even fertilize their own to guarantees offspring. Phylum Platyhelminthes: Taenia 1. Observe slide of Taenia. Noticed the scolex and the body composed of units called proglottids which are not segments, but units formed by budding behind the scolex. proglottids Phylum Platyhelminthes: Taenia 1. Observe slide and model of Taenia scolex which is equipped with suckers and hooks. Note the neck from which new proglotids are budded off. A= suckers B=hooks scolex Phylum Platyhelminthes: Taenia 2. Observe slide and model of Taenia mature proglotid. They have reproductive organs: testículos, vaso deferente, poro genital, glandula de yema, ovario, vagina, utero, cordon nervioso, canales excretores. Uterus Phylum Platyhelminthes: Taenia 2. Observe slide and model of of Taenia gravid proglottid, with the uterus fulled with eggs or embryos. Where do you find gravid proglottids? These proglottids break off and shed in the feces of the host. Outside the host the proglottid breaks releasing thousands of infected eggs. Uterus with eggs Phylum Platyhelminthes: Taenia Taenia life cycle Important Links Platyhelminthes http://www.savalli.us/BIO385/Diversity/05.Platyhelminthes.html http://es.wikipedia.org/wiki/Platyhelminthes http://www.pbs.org/kcet/shapeoflife/episodes/hunter.html