BACTERIA Biology Chapter 18.2 1 Bacteria are very small 2 This is a pore in human skin and the yellow spheres are bacteria 3 Bacteria are very small compared to cells with nuclei 4 Bacteria Bacteria compared to a white blood cell that is going to eat it 5 Clean skin has about 20 million bacteria per square inch 6 Evolution/Classification Most numerous on Earth Most Ancient Microscopic Prokaryotes Evolution has yielded many species adapted to survive where no other organisms can. Grouped based on: Structure, physiology, molec. Composition reaction to specific types of stain. Eubacteria= Germs/bacteria Archaebacteria 7 Kingdom Archaebacteria First discovered in extreme environments Methanogens: Harvest energy by converting H2 and CO2 into methane gas Anaerobic, live in intestinal tracts Extreme halophiles: Salt loving, live in Great Salt Lake, and Dead sea. Thermoacidophiles: Live in acid environments and high temps. Hot Springs, volcanic vents 8 Volcanic vents on the sea floor 9 Chemosynthetic bacteria use the sulfur in the “smoke” for energy to make ATP. 10 The red color of this snow is due to a blue-green bacteria 11 Kingdom Eubacteria Can have one of three basic shapes 1. Bacilli – rod-shaped 2. Spirilla – spiral-shaped 3. Cocci – sphere-shaped Staphylococci – grape-like clusters Streptococci – in chains SHOW ME 12 BACTERIA PICS 13 Bacillus bacteria are rod or sausage shaped 14 Coccus bacteria are sphere or ball shaped 15 Spirillium bacteria have a corkscrew shape 16 Diplo-bacteria occur in pairs, such as the diplococcus bacteria that causes gonorrhea 17 Staphylo bacteria occur in clumps, such as this staphylococcus bacteria that causes common infections of cuts 18 Streptobacteria occur in chains of bacteria, such as this streptococcus bacteria that causes some types of sore throats 19 Spirillium bacteria 20 Diplobacillus bacteria 21 Streptococcus bacteria 22 Staphylococcus bacteria 23 The tip of a needle The red and yellow dots are bacteria 24 Gram Stain Gram-positive retain stain and appear purple Have thicker layer in cell wall. Gram-negative do not retain stain and take second pink stain instead. Phylum Shape Motility Metabolism Gram reacion Cyanobacteria Bacilli, Cocci Gliding, some nonmotile Aerobic, Gramphotosynthetic negative autotrophic Spirochetes Spirals Corkscrew Aerobic, and anaerobic; heterotrophic Gram-Pos Bacilli, cocci Flagella; some nonmotile Aer/anaer.; Mostly heterotrophic, gramphotosynthetic positive Proteobacteria Bacilli, cocci, spiral Flagella; some nonmotile Aer/anaer.; heterotrophic, photosynthetic autotrophic Gramnegative Gramnegative 25 STRUCTURE OF BACTERIA Structure Function Cell Wall Protects and gives shape Outer Membrane Protects against antibodies (Gram Neg. Only) Cell Membrane Regulates movement of materials, contains enzymes important to cellular respiration Cytoplasm Contains DNA, ribosomes, essential compounds Chromosome Carries genetic information Plasmid Contains some genes obtained through recomb. Capsule & Protects the cell and assist in attaching cell to Slime Layer other surfaces Endospore Protects cell agains harsh enviornments Pilus Assists the cell in attaching to other surfaces Flagellum Moves the cell 26 No Nucleus-DNA in Cytoplasm 27 Nutrition and Growth Heterotrophic or Autotrophic Some are Photoautotrophs – Use sunlight for Energy Some are Chemoautotrophs. Many are Obligate Anaerobes. Oxygen = Death Ex. Some are Faculatative Anaerobes With or without Oxygen Ex. Clostridium tetani – Tetanus Escherichia Coli Some are Obligate Aerobes Ex.) Mycobacterium tuberculosis Temperature requirements Some are Thermophilic, Some prefer acidic envmt. 28 These heterotrophic bacteria digest oil -remember oil is partially decayed plant and animal cells 29 REPRODUCTION IN BACTERIA (please add to notes) 30 No Nucleus-DNA in Cytoplasm 31 BACTERIA REPRODUCES BY FISSION First the chromosomal DNA makes a copy The DNA replicates 32 NEXT THE CYTOPLASM AND CELL DIVIDES The two resulting cells are exactly the same 33 In addition to the large chromosomal DNA, bacteria have many small loops of DNA called Plasmids 34 Genetic Recombination Nonreproductive Methods bacteria can acquire new genetic material. Characteristic Transformation Conjugation Transduction Method of DNA Across cell wall Transfer and cell membrane of recepient Through a By a virus conjugation bridge between two cells Plasmid transfer Yes Yes Not likely Chromosome transfer No Sometimes No Antibiotic resistance acquired Yes Yes Sometimes 35 TRANSFORMATION This plasmid of DNA is new to the bacteria – added by transformation! Produces the glowing protein 36 CONJUGATION 37 TRANSDUCTION 38 Bacteria and Disease Disease Pathogen Areas affected Mode of transmission Botulism Clostridium botulinum Nerves Improperly preserved food Cholera Vibrio cholerae Intestine Contaminated water Dental Caries Streptococcus mutans, sanguis, salivarius Teeth Environment to mouth Gonorrhea Neisseria gonorrhoeae Urethra, fallopian Sexual contact Lyme disease Berrelia burgdorferi Skin, joints Tick bite Rocky Mountain SF Rickettsia recketsii Blood, skin Tick bite Salmonella Salmonella Intestine Contaminated food, water Strep throat Streptococcus pyogenes URT, blood, skin Sneezes, coughs, etc. Tetanus Costridium tetani Nerves Contaminated wounds Tuberculosis Mycobacterium tuberculosis Lung, bones coughs 39 Some bacteria cause diseases -Disease causing bacteria are call PATHOGENIC 40 Helicobacter pylori is the pathogenic bacteria that can causes ulcers 41 Leprosy is a bacterial infection that decreases blood flow to the extremities resulting in the deterioration of toes, ears, the nose and the fingers. 42 BOTULISM 43 CHOLERA 44 DENTAL CARIES 45 ROCKY MOUNTAIN SF 46 LYME DISEASE 47 SALMONELLA 48 STREP THROAT 49 TETANUS 50 TUBERCULOSIS 51 Common Antibiotics Antibiotic Mechanism Target bacteria Penicillin Inhibits cell wall synthesis Gram Positive Ampicillin Inhibits cell wall synthesis Broad spectrum Bacitracin Inhibits cell wall synthesis Gram Positive – Skin Ointment Cephalosporin Inhibits cell wall synthesis Gram Positive Tetracycline Inhibits Protein Synthesis Broad spectrum Streptomycin Inhibits Protein Synthesis Gram Neg. tuberculosis Sulfa drug Inhibits cell metabolism Bacterial meningitis, UTI Rifampin Inhibits RNA synthesis Gram Pos., some Neg. Quinolines Inhibits DNA Synthesis UTI 52 Some Final Information Because antibiotics have been overused, many diseases that were once easy to treat are becoming more difficult to treat. Some Bacteria are Useful Ex.) Producing and Processing food Breaking down dead organic material Make unripened cheese like ricotta and cottage by breaking down the protein in milk. 53