CHAPTER 7: CELL STRUCTURE AND FUNCTION California State Standards 1. Cell biology a. Cells are enclosed in semipermeable membranes that regulate their interactions with their surroundings c. Know prokaryotic cell differ from eukaryotic cells e. Know the role of endoplasmic reticulum and golgi apparatus in the secretion of proteins g. Students know the role of mitochondria in making stored chemical-bond energy available to cells j. Students know how eukaryotic cells are given shape and internal organization by a cytoskeleton or cell wall or both. 7-1 Life Is Cellular A. The Discovery of the Cell 1. Early Microscopes • Robert Hooke- Used compound microscope to look at a slice of cork • Anton van Leeuwenhoek- Observed tiny living things in pond water 2. The Cell Theory • Mathias Schleiden-Concluded all plants are made of cells • Theodor Schwann- Stated all animals are made of cells • Rudolf Virchow- Concluded new cells come from existing cells Cell Theory: • All living things are composed of _____ cells • Cells are the basic units of structure and function in living things • New cells are produced from Existing cells ____________ B. Exploring the Cell 1. Electron Microscope (TEM & SEM) -Specimen placed in a vacuum http://www.mos.org/sln/sem/ 2. Scanning Probe Microscope -1990 development of fine probe microscope ordinary air -operates in _______________ -can even show samples in solution C. Prokaryotes and Eukaryotes 0.2 micrometers • cells vary in size from _________________1000 micrometers ___________________ • viruses are not cells Prokaryotic cell Cell membrane Eukaryotic cell Cytoplasm Cell membrane Organelles Cytoplasm Nucleus Prokaryotes Common to Both before pro=__________ contain DNA __________ karyote= kernel (nucleus) Cell generally smaller membrane less complicated no Membrane bound organelles Ex: Kingdom Monera - Eubacteria - Archaea Eukaryotes true Eu= ___________ Karyote=kernel (nucleus) Generally larger Contain membrane bound organelles (“little organs”) Ex: Plants, animals, fungi, and protists Eukaryotic Cell vs. Prokaryotic cell Warm-up Section 7-1 1. Name 3 scientists and their contribution to science. 2. What is the cell theory? 3. What are the characteristics of prokaryotes and eukaryotes. Use the “thinking visually” box on page 173 to answer this question. Venn Diagrams 7-2 Eukaryotic Cell Structure(chart) Section 7-2 Prokaryotes Eukaryotes Cell membrane Contain DNA ribosomes Animal Cells Centrioles Lysosomes Plant Cells Cell membrane Ribosomes Nucleus Endoplasmic reticulum Golgi apparatus Vacuoles Mitochondria Cytoskeleton Cell Wall Chloroplasts Central Vacuole Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton artists rendition of the plant cell Section 7-2 Smooth endoplasmic reticulum Vacuole Ribosome (free) Chloroplast Ribosome (attached) Cell Membrane Nuclear envelope Cell wall Nucleolus Golgi apparatus Nucleus Mitochondrion Rough endoplasmic reticulum Plant Cell artists rendition of an animal cell Animal cell Cell membrane Endoplasmic reticulum Microtubule Microfilament Ribosomes Mitochondrion Warm-up Section 7-2 1. 2. 3. 4. 5. Compare and contrast plant and animal cells. Are they prokaryotic or eukaryotic? Eukaryotic cells maintain shape and internal organization with ….? What is the function of the nucleus? Where is chemical energy from food converted to useable energy? Where is light energy converted to chemical energy in plants? What is this process called? 7-3 Cell Boundaries A. Cell Membrane • Regulates what ___________ and __________ enters leaves the cell • Provides protection and support lipid bilayer • Composed of a ______________ • __________________ are embedded in the Protein molecules bilayer; form channels and pumps to move material across the cell membrane. • _________________ are attached to some of the Carbohydrates proteins; allows cells to __________ one identify another. Outside of cell CELL MEMBRANE “FLUID MOSAIC” Carbohydrate chains Proteins “ID tags” Cell membrane Inside of cell (cytoplasm) Protein channel “transport” Lipid bilayer “Phospholipids” B. Cell Walls • Found in plants, algae, fungi and many prokaryotes • Cell walls are porous enough to allow water, oxygen, carbon dioxide easily • Main function is to provide support and protection for the cell cellulose • Plant cell walls made of __________ (carbohydrate fiber) C. Diffusion Through Cell Boundaries •All living cells exists in a liquid environment. •Cell membranes regulate the movement of molecules in and out of the cell. selectively permeable •Cell membranes are _____________________ semipermeable (aka: _______________) •If substances can pass, then the cell membrane = Permeable •If substances cannot pass, then the cell membrane= impermeable 1. Measuring Concentration • Cytoplasm= Water and other substances between the cell membrane and the nucleus. • Concentration= mass of solute/volume of solution • Example:12g salt/3L H2O= 4 g/L 60g salt/3L H2O= 20 g/L 5X more concentrated • Concentration gradient= unequal distribution of particles 2. Diffusion • Particles constantly collide and spread out randomly in solution high concentration _____ to a ____ low • Particles move from a _____ concentration (with the concentration gradient); process is diffusion known as _________ • Diffusion continues until equilibrium is reached • Equilibrium = When particles are evenly distributed in solution • Diffusion depends upon random particle movements, without requiring the substances diffuse across membranes ______ cell to use energy _______ • The movement of particles will continue to move equally across the cell membrane to maintain equilibrium. D. Osmosis • H2O passes easily across most membranes • Osmosis = The diffusion of water through a selectively permeable membrane Before Osmosis Selectively permeable membrane After Osmosis water solute Figure 7-15 Osmosis 1. How Osmosis Works Section 7-3 Left Beaker: left side (low water • More sugar molecules on the ____ concentration). • High water concentration on the right side. • The membrane is permeable to water but not sugar. • Net movement of water from high water concentration to low water concentration. TYPES OF SOLUTIONS Hypertonic Solution “above strength” High concentration of Dissolved substances In Solution Isotonic Solution “same strength” Hypotonic Solution “below strength” Equal concentration of dissolved substances Low concentration of dissolved substances in solution Cells in a hypertonic solution In a hypertonic solution, water leaves a cell by osmosis, causing the cell to shrink. H2O H2O Water Molecule Dissolved Molecule Cells in a hypotonic solution In a hypotonic solution, water enters a cell by osmosis, causing the cell to swell. H2O H2O Water Molecule Dissolved Molecule Cells in an isotonic solution In an isotonic solution, the concentration of dissolved substances in the solution is the same as the concentration of dissolved substances inside the cell. H 2O H2O Water Molecule Dissolved Molecule 2. The effects of Osmosis on cells (animal cells refer to figure 7-16) Cells in a hypertonic solution Cells in an isotonic solution Cells in a hypotonic solution E. Facilitated Diffusion • Molecules, that cannot diffuse across the cell membrane’s lipid bilayer on their own, can move With the help of protein channels Facilitated Diffusion move from high to low concentration • Molecules still ___________________ Section 7-3 Glucose molecules High Concentration Cell Membrane Low Concentration Protein channel F. Active Transport • Movement of molecules against a concentration gradient (from low to high) • Requires energy • Needs a transport protein or pump Molecule to be carried Energy Molecule being carried Large molecules and clumps of material can be taken into the cell by a process known as ___________. endocytosis The two kinds of endocytosis are: 1. Phagocytosis= ‘Cell eating’ 2. Pinocytosis= ‘cell drinking’ Exocytosis release of large amounts of material ___________= endo/exocytosis demonstration Transport of Large Particles • Endocytosis is a process by which a cell surrounds and takes in material from its environment. Nucleus Digestion Exocytosis Endocytosis Wastes removal 1. interactive sites for cell membrane, diffusion, active, passive transport Let’s Review: PASSIVE TRANSPORT 1.Simple diffusion (includes Osmosis) * No ATP Hi-Lo No protein ACTIVE TRANSPORT 2. Facilitated Diffusion * No ATP * ATP required Hi-Lo Lo-Hi Needs protein Needs Protein Warm-up Section 7-3 1. 2. 3. 4. 5. 6. Describe the anatomy of a cell membrane. What are the main functions of the cell membrane and the cell wall? What happens during diffusion? What is osmosis? Describe the terms hypotonic, hypertonic, isotonic. Compare and contrast passive and active transport. 7-4 The Diversity of Cellular Life A. Unicellular Organisms (single celled) • Unicellular organisms _________ outnumber multicellular organisms • Examples: Yeast, algae, bacteria B. Multicellular Organisms (many celled) • Cells become ___________ specialized to perform different tasks • Cells need to communicate and cooperate C. Levels of Organization • The levels of organization in a multicellular organism are: individual CELLS Se ction Muscle cell TISSUES Sm ooth muscle ( ti ssue) ORGANS Stom ach (organ) ORGAN SYSTEMS Digesti ve system (organ system ) 1. Tissues= Group of similar cells that perform a particular function • Four types of tissue: - muscle - epithelial - nervous - connective 2. Organs= Groups of tissues • Ex. bicep muscle is mad of muscle, connective, and nervous tissue 3. Organ Systems= Group of organs that work together to perform a specific function. Warm-up Section 7-4 1. What are the levels of organization in a multicellular organism? 2. What is cell specialization? Provide 3 examples.