Central Dogma of Biology DNA→RNA→Protein Introduction • DNA info is in the form of specific sequences of bases along the DNA strands. • The DNA leads to specific traits by dictating the synthesis of proteins. • Proteins are the links between genotype and phenotype. – For example, Mendel’s dwarf pea plants lack a functioning copy of the gene that specifies the synthesis of a key protein, gibberellins. – Gibberellins stimulate the normal elongation of stems. One Gene - One Polypeptide • George Beadle and Edward Tatum were to establish the link between genes and enzymes in their exploration of the metabolism of a bread mold, Neurospora crassa. • Their results provided strong evidence for the one gene - one enzyme hypothesis. • Later research refined the one gene - one enzyme hypothesis. • First, it became clear that not all proteins are enzymes and yet their synthesis depends on specific genes. – This tweaked the hypothesis to one gene - one protein. • Later research demonstrated that many proteins are composed of several polypeptides, each of which has its own gene. • Therefore, Beadle and Tatum’s idea has been restated as the one gene - one polypeptide hypothesis. DNA vs RNA • Components of DNA – Sugar (deoxyribose) – Base (A,G,C,T) – Phosphate group • Components of RNA – Sugar (ribose) – Base (A,G,C,Uracil) • RNA does not contain thymine – Phosphate group DNA vs RNA continued • Structural Characteristics of DNA – Double stranded – Base-pairing rules apply (A:T & G:C) • Structural Characteristics of RNA – Primarily single stranded – Limited base-pairing (G:C & A:U) Types of RNA • Messenger RNA (mRNA) – Complementary to info in DNA strand – Variable in length – Contains specific structural info for the sequence of amino acids – Processed before using Types of RNA continued • Transfer RNA (tRNA) – Multiple varieties, each specific for a specific amino acid – Relatively small, with a consistent 3-d shape – Specificity for each amino acid is accomplished by a triplet base-pairing relationship between codon on mRNA and anti-codon on tRNA Transcription and translation are the two main processes linking gene to protein: an overview • Genes provide the instructions for making specific proteins. • The bridge between DNA and protein synthesis is RNA. • RNA is chemically similar to DNA, except that it contains ribose as its sugar and substitutes the nitrogenous base uracil for thymine. – An RNA molecules almost always consists of a single strand. DNA→RNA→Protein • DNA is TRANSCRIBED to messenger RNA (mRNA) • mRNA carries the message to tranfer RNA (tRNA) • tRNA is TRANSLATED to an amino acid chain, which makes up proteins • In DNA or RNA, the four nucleotide monomers act like the letters of the alphabet to communicate information. • The specific sequence of hundreds or thousands of nucleotides in each gene carries the information for the primary structure of a protein (the linear order of the 20 possible amino acids) • To get from DNA, written in one chemical language, to protein, written in another, requires two major stages, transcription and translation. • During transcription, a DNA strand provides a template for the synthesis of a complementary RNA strand. – This process is used to synthesize any type of RNA from a DNA template. – Transcription is from the 3’→5’ strand (template strand) • Transcription of a gene produces a messenger RNA (mRNA) molecule. – mRNA carries the message from the nucleus to the ribosomes • During translation, the information contained in the order of nucleotides in mRNA is used to determine the amino acid sequence of a polypeptide. – Translation occurs at ribosomes. • The basic mechanics of transcription and translation are similar in eukaryotes and prokaryotes. • Because bacteria lack nuclei, transcription and translation are coupled. • Ribosomes attach to the leading end of a mRNA molecule while transcription is still in progress. Fig. 17.2a • In a eukaryotic cell, almost all transcription occurs in the nucleus and translation occurs mainly at ribosomes in the cytoplasm. • In addition, before the primary transcript can leave the nucleus it is modified in various ways during RNA processing before the finished mRNA is exported to the cytoplasm. – Introns are removed Fig. 17.2b RNA Packaging • Introns are removed (only exons contain genetic info) • Addition of a 5’ cap on mRNA (for orientation purposes) • Addition of a 3’ tail on mRNA (allows it to last longer) • To summarize, genes program protein synthesis via genetic messenger RNA. • The molecular chain of command in a cell is DNA → RNA → protein. This is referred to as the Central Dogma of Biology