Unit 8 test
Biotech study guide
Test format
• 20 questions
– Know vocabulary
– Be able to place the steps for recombinant DNA
technology
• 1 open-ended (10 points)
– Be able to read a DNA fingerprint
– Explain the process of gel electrophoresis
– How this technology is used to solve crimes
What kind of cells are used to make animal
clones?
What kind of cells are used to make animal
clones?
Body cells and egg cells
This is a unique gel electrophoresis
banding pattern produced by a
person’s restriction fragments
This is a unique gel electrophoresis
banding pattern produced by a
person’s restriction fragments
DNA fingerprint
Similar to DNA replication because
it makes lots of copies of the DNA
molecule
Similar to DNA replication because
it makes lots of copies of the DNA
molecule
PCR
Small circular DNA molecules that
are naturally found in bacteria
Small circular DNA molecules that
are naturally found in bacteria
plasmids
Knowing the sequence of an
organism’s DNA allows scientists to
study…
Knowing the sequence of an
organism’s DNA allows scientists to
study…
specific genes
This is combining genes from
different sources into a single DNA
molecule
This is combining genes from
different sources into a single DNA
molecule
recombinant DNA
What process separates DNA
fragments by their size and charge
What process separates DNA
fragments by their size and charge
gel electrophoresis
During genetic modification, the
organism’s cells take in ________
from another ___________.
During genetic modification, the
organism’s cells take in __DNA____
from another __source____.
What was added to
the blood samples
to create these
fragments?
What was added to
the blood samples
to create these
fragments?
Restriction enzymes
What is this process?
What is this process?
Gel electrophoresis – be able to
explain this process
Who committed the crime?
Who committed the crime?
Suspect 2
What would be possible sources for
these DNA samples?
What would be
possible sources for
these DNA samples?
Blood, hair, skin cells,
bodily fluids
What is the correct order?
____ Insert recombinant DNA into bacteria cells
____ Isolate desired product from bacteria cells
____ Cut plasmid and desired gene with the same
restriction enyzmes
____ Clone bacteria cells containing recombinant
DNA
____ Combine plasmid and gene fragment, creating
recombinant DNA
What is the correct order?
__3_ Insert recombinant DNA into bacteria cells
__5_ Isolate desired product from bacteria cells
__1_ Cut plasmid and desired gene with the same
restriction enyzmes
__4_ Clone bacteria cells containing recombinant
DNA
__2_ Combine plasmid and gene fragment, creating
recombinant DNA
What was used to cut this piece of
DNA?
What was used to cut this piece of
DNA?
Restriction enzymes
What are C & D?
What are C & D?
sticky ends
If a scientists wants to make a
human protein (like insulin) using
bacteria cells – what would be
their first step?
If a scientists wants to make a
human protein (like insulin) using
bacteria cells – what would be
their first step?
Use a restriction enzyme to cut out
the human gene and the plasmid
A stretch of DNA that varies among
individuals is called a…
A stretch of DNA that varies among
individuals is called a…
genetic marker
In this gel sample, band 3 moved
further than all the other bands.
Why?
In this gel sample,
band 3 moved further
than all the other
bands. Why?
Moved faster because
it is smaller than other
pieces
What are some advantages to
transgenic (GMO) plants?
What are some advantages to
transgenic (GMO) plants?
More nutrients
resist pests
resist herbicides
longer shelf-life