BIOTECHNOLOGY STUDY GUIDE (H) Major Learnings: There are distinct patterns in the organization of nature. Essential and Guiding Questions: 1. How does biotechnology affect society and the environment? Be able to: Model the steps of recombinant DNA technology. Model the steps of somatic cell cloning. Interpret a DNA fingerprint. Discuss the ethical considerations of genetic engineering. Describe the process of differentiation and how this process relates to gene activation or deactivation. Explain how genetic engineering has impacted the fields of medicine, forensics and agriculture (eg., selective breeding, gene splicing, cloning, genetically modified organisms, gene therapy) VOCABULARY TERMS Blastocyst Polymerase Chain Reaction (PCR) Differentiation Polyploidy DNA Fingerprint Radiation/Chemical Induced Mutations DNA Ligase Recombinant DNA Enucleated Egg Reproductive Cloning Epigenetic Marks Restriction Enzymes Epigenetics Selective Breeding Epigenome Somatic Cell Gel Electrophoresis Somatic Cell Nuclear Transfer (SCNT) Gene Cloning/Splicing Stem Cells Gene Therapy Sticky Ends Genetically Modified Organism (GMO) Surrogate Hybrid/hybridization Therapeutic Cloning Inbreeding Transformation Induced Pluripotent Stem Cells (iPS) Transgenic Organism Inner Cell Mass (ICM) Vector Plasmid