Chapter 20: Biotechnology
Concept 20.1: DNA cloning yields multiple copies of a gene or other DNA segment
20.2 A preview of gene cloning and some uses of cloned genes
20.3
Using a restriction enzyme and DNA ligase to make recombinant DNA
20.4
Cloning a human gene in a bacterial plasmid: Research Method Box
20.6
Making complementary DNA (cDNA) for a eukaryotic gene
20.7
Detecting a specific DNA sequence by hybridization with a nucleic acid probe: Research Method
20.8
The polymerase chain reaction (PCR): Research Method
Concept 20.2: DNA technology allows us to study the sequence, expression, and function of a gene
20.9 Gel electrophoresis: Research Method
20.10 Using restriction fragment patterns to distinguish DNA from different alleles
20.12 Dideoxy Chain Termination method for sequencing DNA (Sanger Method): Research Method
20.15 DNA microarray assay of gene expression levels: Research Method
Concept 20.3: Cloning organisms may lead to production of stem cells for research and other
applications
20.21 Single nucleotide polymorphism (SNPs) as genetic markers for disease-causing alleles