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Overview of A&P1 Labs
Lab Syllabus
Text is lab manual – bring it to each lab
Web of Life – Lab Worksheets/Resources
Quizzes – weekly, oral/written format
– Do not be late – questions not repeated
– No Make-ups
– 12 taken, 10 best averaged
• Work as a team with your table
• Access to Lab
• Clean up
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Lab 1
• Membrane transport processes
• Body Cavities, Abdominopelvic Quadrants,
and Organs
• Organ locations
• Directional Terminology
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Membrane Transport Processes
• What membrane are we talking about?
• Why is it important to transport materials
across the membrane?
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Membrane Transport
• Plasma membranes selectively
permeable
– Some molecules pass through easily; some
do not
• Two ways substances cross membrane
– Passive processes
– Active processes
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Types of Membrane Transport
• Passive processes
– No cellular energy (ATP) required
– Substance moves down its concentration
gradient
• Active processes
– Energy (ATP) required
– Occurs only in living cell membranes
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Passive Processes
• Two types of passive transport
– Diffusion
• Simple diffusion
• Carrier- and channel-mediated facilitated diffusion
• Osmosis
– Filtration
• Usually across capillary walls
• The ones we will do in lab today are
diffusion, osmosis and filtration
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Passive Processes: Diffusion
• Collisions cause molecules to move down
or with their concentration gradient
– Difference in concentration between two
areas
• Speed influenced by molecule size and
temperature
• This can occur in a air, water and even
though a membrane
• Video clip
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Passive Processes
• Molecule will passively diffuse through
membrane if
– It is lipid soluble
• oxygen, carbon dioxide
– Small enough to pass through membrane
channels
– Assisted by carrier molecule
PLAY
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Animation: Membrane Permeability
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Figure 3.7a Diffusion through the plasma membrane.
Extracellular fluid
Lipidsoluble
solutes
Cytoplasm
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Simple diffusion of
fat-soluble molecules
directly through the
phospholipid bilayer
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Passive Processes: Osmosis
• Movement of solvent (e.g., water) across
selectively permeable membrane
• Water diffuses through plasma
membranes
– Through lipid bilayer
– Through specific water channels called
aquaporins (AQPs)
• Occurs when water concentration different
on the two sides of a membrane
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Figure 3.7d Diffusion through the plasma membrane.
Water
molecules
Lipid
bilayer
Aquaporin
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Osmosis, diffusion of a
solvent such as water
through a specific
channel protein
(aquaporin) or through
the lipid bilayer
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Passive Processes: Osmosis
• Water concentration varies with number of
solute particles because solute particles
displace water molecules
• Osmolarity - Measure of total
concentration of solute particles
• Water moves by osmosis until hydrostatic
pressure (back pressure of water on
membrane) and osmotic pressure
(tendency of water to move into cell by
osmosis) equalize
• Osmosis Video
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Passive Processes: Osmosis
• When solutions of different osmolarity are
separated by membrane permeable to all
molecules, both solutes and water cross
membrane until equilibrium reached
• When solutions of different osmolarity are
separated by membrane impermeable to
solutes, osmosis occurs until equilibrium
reached
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Figure 3.8a Influence of membrane permeability on diffusion and osmosis.
Membrane permeable to both solutes and water
Solute and water molecules move down their concentration gradients
in opposite directions. Fluid volume remains the same in both compartments.
Left
compartment:
Right
compartment:
Solution with
Solution with
lower osmolarity greater osmolarity
Both solutions have the
same osmolarity: volume
unchanged
Solute
Solute
molecules
(sugar)
Freely
permeable
membrane
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Figure 3.8b Influence of membrane permeability on diffusion and osmosis.
Membrane permeable to water, impermeable to solutes
Solute molecules are prevented from moving but water moves by osmosis.
Volume increases in the compartment with the higher osmolarity.
Left
compartment
Right
compartment
Selectively
permeable
membrane
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Both solutions have identical
osmolarity, but volume of the
solution on the right is greater
because only water is
free to move
Solute
molecules
(sugar)
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Tonicity
• Tonicity: Ability of solution to alter cell's
water volume
– Isotonic: Solution with same non-penetrating
solute concentration as cytosol
– Hypertonic: Solution with higher nonpenetrating solute concentration than cytosol
– Hypotonic: Solution with lower nonpenetrating solute concentration than cytosol
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Figure 3.9 The effect of solutions of varying tonicities on living red blood cells.
Isotonic solutions
Cells retain their normal size and
shape in isotonic solutions (same
solute/water concentration as inside
cells; water moves in and out).
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Hypertonic solutions
Cells lose water by osmosis and shrink
in a hypertonic solution (contains a
higher concentration of solutes
than are present inside the cells).
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Hypotonic solutions
Cells take on water by osmosis until they
become bloated and burst (lyse) in a
hypotonic solution (contains a lower
concentration of solutes than are
present inside cells).
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Lab 1 - Procedure
• Start Osmosis procedure A or B – not both –
takes about an hour to complete
• Start diffusion demo – takes about 30 min to
complete
• Group Work – I hr
– Learn internal organs/ abdominopelvic
quadrants with mannequins
– Learn Directional Terminology
• Start filtration demo
• Read results of osmosis and diffusion demos
and answer questions
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