LAB 5
Cellular Respiration
http://www.phschool.com/science/biology_place/labbench/lab5/process.html
C 6H 12 O6 + 6O2 → 6 H 2 O + 6 CO 2
• KOH turns CO2 gas into a solid
• So changes in volume will be due to ?
http://www.phschool.com/science/biology_place/labbench/lab5/respwork.html
CONTROL
• If you want to control variables the contents of the
vials must start the SAME
• How can you measure
the volume of an
irregularly shaped
object, like peas?
Make another set
1, 2, 3 will go in room temp; 4, 5, 6 will go in 10° water
http://www.phschool.com/science/biology_place/labbench/lab5/assemble.html
• Place respirometers in water bath with tips of pipettes
resting on a masking tape sling above the water level.
• Wait 7 MINUTES for temperature to equilibrate.
• Add a drop of RED FOOD COLOR into the tip of each pipette
http://www.phschool.com/science/biology_place/labbench/lab5/measure.html
•
•
•
•
•
Lower the pipettes into water
Quickly align the tubes so you can read the numbers
Wait 3 MINUTES
Record level of water in each pipette and start timer
Take readings from each respirometer at 5 minute
intervals for 20 minutes
• REPEAT with 2nd set of respirometers in ice water
http://www.phschool.com/science/biology_place/labbench/lab5/measure.html
http://www.phschool.com/science/biology_place/labbench/lab5/quiz.html
HOW DOES TEMPERATURE AFFECT
THE RESPIRATION RATE IN AN ORGANISM?
Cold blooded
(Ectothermic)
http://www.lbreptileexperience.com/adventures.html
Warm blooded
(Endothermic)
http://www.uselessgraphics.com/rats.htm