Bellwork
(8 minutes only)
1. What makes
this compound
microscope
different from
the dissection
microscopes
we’ve used?
2.
Copy the
labels onto
your handout
3.
Organize 3
ring binder (all
in order) &
review for test
Essential Question
How do the interactions of
living things and the
environment affect our world?
_______(old answer)__________
____________________________
____________________________
____________________________
NEW & IMPROVED ANSWER:
____________________________
____________________________
____________________________
____________________________
____________________________
Ecology Unit
1. Notes
 Pull
out the ecology Divider
 +2pts
each assignment
present
 Flip
through notebook &
score as Ms. D puts
assignments on board
 Circle
score at top (24 pts
possible). Pass forward.
-What is Life Notetaker
-Energy Flow Notes
-Trophic Levels & Rule of 10 Notes
-Biome Poster Notes/Research Notes
-Aquatic Macroinvertebrates & Water Quality
-Population Notes
2. Labs
-Cricket Mark & Recapture Lab
-Aquatic Macroinvertebrates Lab
-Jackrabbits & Coyotes Lab
3. Assignments
-Critter Outline
-Overpopulation Article
4. Tests
-What Is Life Quiz
 Take
only from appropriate cabinet
 Always carry with 2 hands (base & arm)
 ONLY use lens paper to clean
 Do NOT force knobs
 Keep away from table edge &
keep objects clear of cords
 Store covered & w/ stage down
aka “put it in park”
1.
2.
3.
4.
5.
6.
7.
Place slide on Microscope
Use Stage Clips
Click Nosepiece to the lowest
(shortest) objective
Look into Eyepiece
Turn Coarse Adjustment (big knob) to focus;
move slide if nessary
Click to medium objective
Use Coarse & Fine Adjustments (big & little
knobs) to focus
8. Click the nosepiece to the high power
(longest) objective
9. Do NOT use the Coarse Focus Knob anymore
10. Use ONLY the Fine Focus Knob (little knob)
to focus on high power
What can you find on your slide?
Bellwork
1.
How can you tell the difference between low,
medium, & high power objectives?
2.
Summarize the steps to focusing a microscope in
3-4 sentences (look @ your microscope notes for
assistance)
3.
How do you put a microscope into PARK?
4.
What safety rule do you need to remember when
using HIGH power?
**When finished, study your microscope parts
diagram, short scratch paper quiz today!
Microscope Quiz
For 1-5, name
the part
being pointed
to in the
picture
6. Which power objective must you focus in FIRST?
7. What is the ONLY knob you should use when
focusing on high power?
8. To put the microscope in park @ the end, you must
cover it and put the stage all the way __________.
6. Which power objective must you focus in
FIRST? LOW
7. What is the ONLY knob you should use when
focusing on high power? FINE FOCUS
8. To put the microscope in park @ the end,
you must cover it and put the stage all the
way DOWN
Cells Unit
1. Notes
-Microscope Notes
2. Labs
3. Assignments
4. Tests
Essential Question
Why are cells called the
“basic unit of life”?
_______________________
_______________________
_______________________
_______________________
_______________________
 Title
(what is it, what power lens)
 Size (petri dish)
 Detail (Slow down! Count! Again!)
 Observations (complete sentences)
 Labels (use ID guides & vocab)
U of A pond water, high power
Observations
•The algae is light green
inside
•The cells are all about the
same size rectangles
•There are 16 strands of
algae in view
Green algae
Rhizoclonium
Plant cell
(Eukaryote)
Question: What do cells look like under a
microscope?
Hypothesis: If…then…as measured by….
Procedures:
1. On HIGH power, draw 2+ QUALITY
microscope drawings.
Data (drawings):
Title- 2pts (must describe sample AND power)
Size- 1pt (at least as big as a medium petri dish)
Detail- 3pts (3=very detailed, 2=some detail, 1=scribbles)
Observations- 3pts (3=two+ observations in
complete sentences; 2=two+ observations, but
incomplete sentences; 1=only on observation)
Labels- (1pt) at least one label on the drawing
 Turn
in critter project
Put new assignment & rubric on LEFT side
pocket (new assignment side)
1.
On a scale of 1 (best) to 3 (worst), where do
you rate your ability to focus on high power?
2.
Explain why you gave yourself that rating.
3.
Answer the question below based on your
rating:

If you gave yourself a 1 what specific things can you
do next class period to be a leader and assist students
who need more help?

If you gave yourself a 2 or a 3, what specific things
will you try doing next class period to gain confidence
and practice?
1.
Look back at yesterday’s
hypothesis did the cells
you saw yesterday look
like what you expected
them to? Explain.
2.
What is required for a
microscope drawing? (list
ALL parts)
3.
Set up your lab notebook
for today like picture on
the right
Prokaryote vs Eukaryote Lab
Background Info:
Prokaryote = bacteria cells
Eukaryote = plant & animal cells
Question:
What differences can you see between
prokaryotic cells and eukaryotic cells under
the microscope?
 Science


Fair Proposal
Approved: You earned half of your points. Staple
a lined piece of paper to back addressing ALL
comments to earn the rest.
Not approved: You earned 0 pts and must staple
lined sheet of paper with new proposal to earn
any points back. DUE MONDAY!
 In
computer lab ROOM 38 (DIFFERENT!) next
Tuesday to do experimental design in rough
draft packet
 Turn
in critter project (Bio 1-2 turn in today)
 Pre-AP- attach late pass to turn in today
Put new assignment & rubric on LEFT side
pocket (new assignment side)
Stained MAMMAL TISSUE slides typically
show the nucleus as a small, dark spot
did you see these yesterday?
 Prokaryote=
 Eukaryote=
bacteria cells
plant & animal cells
Question: What differences can you see
between prokaryotic and eukaryotic cells
under the microscope?
Hypothesis:
If
Then
As Measured By
Make a prediction about size, shape, or other
characteristic you can SEE
1.
View prepared slide of prokaryotes
(1 HIGH POWER drawing of CELLS
required)
2.
Prepare wet mount of pond water
& view eukaryotic cells (1 HIGH
POWER drawing of CELLS required)
-Label slide w/ masking tape
-Put 1-2 drops pond water on clean
slide
-Put cover slip on top
3. Clean-up
-Rinse slides & cover slips @ BOTTOM
of sink (in case you drop)
-GENTLY dry slides w/ paper towel
-Sanitize table & dry
-Wash hands
 Microscope



Takes microscope ½ sheet
ONLY fine focus knob used on high power
Keeps microscope away from edge
 Slides



Expert
Safety Expert
Makes sure everyone is being PAYING ATTENTION
while preparing & cleaning slides
Makes sure slides are labeled
Alerts Ms. D immediately of any breaks
What did you learn about prokaryotes vs
eukaryotes? Explain.
Summarize your hypothesis and say whether it
was supported or rejected?
What observations supported or rejected your
hypothesis? (give details)
What did you learn about prokaryotes vs
eukaryotes? Explain.
1.
Sketch a prokaryotic cell & a eukaryotic
cell.
2.
What were the major differences you saw
between the two cell types?
3.
Finish your 3-4 sentence conclusion for
yesterday’s lab- IN YOUR NOTEBOOK:
-Was your hypothesis supported or
refuted?
-What observations supported or
refuted your hypothesis (explain)
-What did you learn about
prokaryotes & eukaryotes?
Cells
are divided into 2
major categories based on
how the DNA is stored:
Prokaryotes or Eukaryotes
Remember
DNA? It’s what
codes for your
GENES and
makes you
who you are!
 NO
nucleus to store DNA, it floats coiled
in the middle of the cell
 Very small and simple structure
 Example: Bacteria
DNA
Draw and label
the DNA on your
diagram
 DNA
stored in nucleus surrounded by
a protective membrane
 Larger size, complex structure
 Examples: plants and animals
Draw and label
the DNA on your
diagram
DNA
http://learn.genetics.utah.edu/content/begin/cells/scale/
Talk to your
partner & be
ready to share
 Organelles
are structures that work like
miniature organs within the cell to perform
specific functions (jobs). ONLY found in
eukaryotes.
 Contains
the genetic material (DNA/genes)
 Surrounded & protected by a nuclear membrane
 Directs all the activities of the cell.
 Analogy = the “brain” or “jefe” of the cell.
Do prokaryotes
have a nucleus?
NO!!!!
NUCLEUS
NUCLEAR
MEMBRANE
 Convert
sugars into ATP energy (ATP is
the chemical used to power the cell)
 They have their own DNA! They probably
came from a eukaryote engulfing a
PROKARYOTE!
 Analogy = “Powerhouse of the cell”
MITOCHONDRIA
A
gel-like substance that fills the cell
(mostly made of water)
 Materials in the cell are suspended in the
cytoplasm
 Analogy = “the glue that holds everything
in place”
Cytoplasm
Cytoplasm
 Separates
& protects the cell from the
outside environment
 It is SEMI-PERMEABLE (let’s some things
in, keeps other things out)
 Analogy = “It’s the bouncer of the cell”
Cell Membrane
Cell Membrane
A
tough, rigid, outer cell layer (outside the cell
membrane)
 Provides extra strength; helps cells keep their
shape
 Found in plants & bacteria, NOT in animal cells
 Analogy = “Spanx of the cell- keeps the shape”
Cell Wall (outer
most layer)
If we’re not labeling
a cell wall on our
eukaryote…does that
make it a plant or
animal cell?
ANIMAL!!!!
 Use
the textbook
Chp. 3.1-3.3 to
fill out your cell
parts worksheet
 Make
a Venn Diagram for Prokaryotes vs Eukaryotes
and decide where in the Venn Diagram to write
each of the following terms:
Nucleus
Mitochondria
Bacteria
Prokaryotes
Cell Wall
Cytoplasm
Plants
Cell Membrane
Animal
Organelles
Eukaryotes
1.
What is 1 major difference between eukaryotes and
prokaryotes?
2.
Look at your notes from yesterday- what 2
ORGANELLES did you learn about? What was the
analogy for each one?
3.
Which organelle would you expect a cell to have only
ONE? Which organelle would there be many of in the
same cell?
4.
Title the next page in your notebook “Eukaryotic Cells
Lab: Plant vs Animal”
5.
Copy the Question: “If plant and animal cells are both
eukaryotic, how do they compare under the
microscope?”
6.
Write an If…then…as measured by…. Hypothesis. Be
ready to share!
 Always



label the slides you prepare!
Use masking tape & pen
Put label on TOP of slide
Include DATE and DESCRIPTION
10-1-12
Jessica’s
cheek
cells
A. Prepare a slide of your cheek cells
1.
2.
3.
4.
5.
Put 1 drop methylene blue on labeled slide
GENTLY scrape inside of cheek w/ toothpick 5-6 times
Swirl toothpick into methylene blue on slide
Place cover slip on top
Clean upLeave slide & cover slip in bleach solution 1-2 mins. before rinsing
& drying
B. View 2 different prepared slides of mammal tissue
C. Prepare a wet mount of an onion skin
1.
2.
3.
4.
Peel off a THIN layer of onion skin
Stretch the skin across slide
Put 1 drop iodine stain on top of onion skin
Place a cover slip on top
D. Prepare a wet mount of an elodea leaf
1.
2.
Put one wet elodea leaf onto slide
Place a cover slip on top
E. View 1 prepared plant slide (come get from Ms. D)
 Microscope



Takes microscope ½ sheet
ONLY fine focus knob used on high power
Keeps microscope away from edge
 Slides



Expert
Safety Expert
Makes sure everyone is being PAYING ATTENTION
while preparing & cleaning slides
Makes sure slides are labeled
Alerts Ms. D immediately of any breaks
1
HIGH power drawings of your CHEEK cells
2
HIGH power drawings of a mammal tissue
slides
1
HIGH power drawing of onion skin cells
1
HIGH power drawing of elodea leaf cells
1
HIGH power drawing of a prepared plant slide
 Which
organelle(s) and cell part(s) did you
see in your cheek cells?
1.
What is due TOMORROW? (look @ science
fair due dates…)
2.
Which of the 6 drawings did you complete
yesterday?
3.
What kinds of labels did you add to your
drawing(s)?
4.
What kind of QUANTITATIVE observations
did you record?
*****When finished, skim through the handout
to learn about an upcoming assignment
Due Mon. October 15th. Turn in early for ex. credit!
1.
3 pages folded in ½ stapled together
2.
Must do a EUKARYOTE.
-Pick either PLANT or ANIMAL cell. Circle on sheet!
3.
Include at least 10 cell parts (look at the
comparison chart in notetaker for possibilities)
4. Compare your cell to something
that has many parts. This is
your title page.
-Example: A cell is like a school
A cell is like a
school
by Ms. D
5. Use the jobs of the cell parts to
figure out how to relate them
to the analogy
-Example: The nucleus directs
all the activities of the
cell…..who directs all the
activities of the school?
-The principal!
Draw a picture & explain the
analogy for each cell part. This
goes on each page.
The principal is like the
NUCLEUS because he directs
the activities of the school,
just like the nucleus directs
the activities of a cell.
A. Prepare a slide of your cheek cells
1.
2.
3.
4.
5.
Put 1 drop methylene blue on labeled slide
GENTLY scrape inside of cheek w/ toothpick 5-6 times
Swirl toothpick into methylene blue on slide
Place cover slip on top
Clean upLeave slide & cover slip in bleach solution 1-2 mins. before rinsing
& drying
B. View 2 different prepared slides of mammal tissue
C. Prepare a wet mount of an onion skin
1.
2.
3.
4.
Peel off a THIN layer of onion skin
Stretch the skin across slide
Put 1 drop iodine stain on top of onion skin
Place a cover slip on top
D. Prepare a wet mount of an elodea leaf
1.
2.
Put one wet elodea leaf onto slide
Place a cover slip on top
E. View 1 prepared plant slide (come get from Ms. D)
 Decide
what you will compare your cell to
(what’s the title of your book?)
 Start
writing down what you could compare
the nucleus, mitochondria, cell membrane &
cytoplasm to….
 Remember,
eukaryotic cells are very complex,
and this category includes both plant and
animal cells.
 They
have many cell parts and organelles, but
there are some differences between plant and
animal cells.
Characteristic/Cell Part
ANIMAL cell
PLANT cell
Round (irregular shape)
Rectangular (fixed shape)
Cell wall
-
+
Plasma (Cell) Membrane
+
+
Cytoplasm
+
+
Mitochondria
+
+
Nucleus
+
+
Smooth Endoplasmic Reticulum
+
+
Rough Endoplasmic Reticulum
+
+
Golgi Apparatus / Golgi Body
+
+
Ribosomes
+
+
Lysosomes
+
Usually -
Microtubules / Microfilaments
+
+
Centrioles
+
Usually -
Sometimes
Sometimes
Cilia
+
Rare
Chloroplast
-
+
+ (small)
+ (VERY large, centrally located)
Overall Shape of Cell
Flagella
Vacuole
 Plastids
are special cell parts that are found
only in plants.
What differences
did you see
looking at the
plant cells?
 Use
energy from the sun to make sugars (food)
for the cell through photosynthesis!
 Contain a green pigment called chlorophyll
 Analogy = the CHEF of the cell! (makes the food)
A
large central fluid-filled sac that regulates
water content & keeps cells “full” & rigid
 HUGE inside plants, can take up 90% of the cell
volume!
 Analogy = the WATER TOWER of the cell!

Write a 3 sentence conclusion for the lab
Was your hypothesis supported or rejected?
What observations supported or rejected your
hypothesis?
 What did you learn about plant vs animal cells (use
vocab)?



Update your table of contents for the Prokaryote
vs Eukaryote Lab AND the Plant vs Animal Cell
Lab

When you are finished, begin labeling your plant
vs animal cell diagram on the back of your note
taker