Lesson Overview Energy and Life Chapter 8: Photosynthesis Lesson Overview Energy and Life Section 8.1: Energy and Life Lesson Overview Energy and Life A. Chemical Energy and ATP a. Adenosine triphosphate (ATP) i. Used to store and release energy in the cell ii. Consists of adenine, a 5-carbon sugar, and 3 phosphate groups Lesson Overview Energy and Life b. Adenosine diphosphate (ADP) i. very similar to ATP ii. Has 2 phosphate groups instead of 3 iii. If a phosphate group is added to ADP, it produces ATP Lesson Overview Energy and Life B. Cells release energy in ATP by breaking bonds between the last two phosphate groups a. ATP is useful for short term energy, but not for long term energy storage b. ADP is like a rechargeable battery in the cell Lesson Overview Energy and Life Section 8.2: Photosynthesis: An Overview Lesson Overview Energy and Life A. Chemical Energy and ATP a. Light Energy i. Sunlight is a mixture of different wavelengths ii. Visible spectrum – red, orange, yellow, green, blue, indigo, and violet Lesson Overview Energy and Life b. Pigments i. pigments: light-absorbing molecules that gather the sun’s energy ii. Chlorophyll: plant’s principle pigment 1. two types of chlorophyll; chlorophyll a and chlorophyll b 2. absorb light in the blue-violet and red regions but not in the green 3. plants reflect green light, which is why plants look green Lesson Overview Energy and Life iii. Carotene: red and orange pigments that absorb light in other regions of the spectrum iv. Chlorophyll is primary pigment, but as temperature drops and chlorophyll breaks down, the red and orange pigments may be seen Lesson Overview Energy and Life c. Chloroplasts i. Organelle in which photosynthesis takes place ii. Thylakoids: saclike photosynthetic membranes that are interconnected and arranged in stacks known as grana iii. Stroma: fluid portion outside thylakoids iv. Pigments are located in thylakoid membranes Lesson Overview Energy and Life B. High-Energy Electrons a. Plants use electron carriers to transport high-energy electrons from chlorophyll to other molecules b. NADP+ accepts and holds two high-energy electron, along with a hydrogen ion (H+) and is converted to NADPH i. NADPH carries the high-energy electrons to chemical reactions elsewhere in the cell Lesson Overview Energy and Life C. An Overview of Photosynthesis a. Photosynthesis uses the energy of sunlight to convert water and carbon dioxide into high-energy sugars and oxygen. In symbols: 6 CO2 + 6 H2O C6H12O6 + 6 O2 In words: Carbon dioxide + Water Sugars + Oxygen Lesson Overview Energy and Life i. Plants use the sugars generated by photosynthesis to 1. Produce complex carbohydrates (starch) 2. Provide energy for the synthesis of other compounds (lipids and proteins) Lesson Overview Energy and Life b. Light-Dependent Reactions i. Require the direct involvement of light and light-absorbing pigments ii. Use energy from sunlight to produce ATP and NADPH iii. Reactions take place within the thylakoid membranes of the chloroplast iv. Water is required as a source of electrons and hydrogen ions v. Oxygen is released as a byproduct Lesson Overview Energy and Life c. Light-Independent Reactions i. Plants absorb carbon dioxide from the atmosphere ii. ATP and NADPH molecules produced in the light-dependent reactions are used to produce high-energy sugars from carbon dioxide iii. No light is required iv. Reactions take place outside thylakoids in the stroma of the chloroplast Lesson Overview Energy and Life Lesson Overview Energy and Life Section 8.3: The Process of Photosynthesis Lesson Overview Energy and Life A. The Light-Dependent Reactions: Generating ATP and NADPH a. photosystems: clusters of chlorophyll and proteins in the thylakoid i. Absorb sunlight ii. Generate high-energy electrons that are passed to a series of electron carriers in the thylakoid membrane Lesson Overview Energy and Life b. Photosystem II i. Light energy is absorbed by electrons in the pigments within photosystem II, increasing the electron’s energy level ii. High-energy electrons are passed to the electron transport chain iii. Electron Transport Chain: series of electron carriers that shuttle high-energy electrons during ATPgenerating reactions Lesson Overview Energy and Life iv. Thylakoid membrane provides new electrons to chlorophyll from water molecules v. Enzymes break up water molecules into 2 elecrons, 2 H+ ions, and 1 oxygen atom vi. The 2 electrons replace the highenergy electrons that have been lost to the electron transport chain vii. Oxygen is released into the air vii. H+ ions are released inside the thylakoid Lesson Overview Energy and Life c. Electron Transport Chain i. Energy from the electrons is used by proteins to pump H+ ions from the stroma into the thylakoid space i. At the end of the electron transport chain, electrons pass to photosytem I Lesson Overview Energy and Life d. Photosystem I i. Pigments in photosystem I use energy from light to reenergize electrons ii. NADP+ molecules in the stroma pick up high-energy electrons and H+ ions at the outer surface of the thylakoid membrane to become NADPH Lesson Overview Energy and Life e. Hydrogen Ion Movement and ATP Formation i. ii. iii. Gradient (difference in both charge and H+ concentration across the membrane) provide energy to make ATP ATPsynthase: protein that spans the thylakoid membrane and allows H+ ions to pass through ATP synthase binds ADP and a phosphate group to produce ATP Lesson Overview Energy and Life f. Summary of Light-Dependent Reactions i. Produce oxygen gas and convert ADP and NADP+ into ATP and NADPH ii. ATP and NADPH provide energy needed to build high-energy sugars from low-energy carbon dioxide Lesson Overview Energy and Life B. The Light-Independent Reactions: Producing Sugars a. Also called the Calvin cycle b. Use energy that ATP and NADPH contain to build stable highenergy carbohydrate compounds that can be stored for a long time Lesson Overview Energy and Life c. Carbon Dioxide Enters the Cycle i. Carbon dioxide enters the Calvin cycle from the atmosphere ii. An enzyme in the stroma combines carbon dioxide with 5-carbon compounds that are already present iii. Produces 3-carbon compounds that continue into the cycle Lesson Overview Energy and Life Carbon Dioxide Enters the Cycle iv. For every 6 carbon dioxide molecules, a total of twelve 3-carbon compounds are produced v. Other enzymes convert the 3-carbon compounds into higher-energy forms, using energy from ATP and NADPH Lesson Overview Energy and Life d. Sugar Production i. 2 of the twelve 3-carbon molecules leave the cycle halfway through ii. Remaining ten 3-carbon molecules are converted back into six 5-carbon molecules that combine with 6 new carbon dioxide molecules to begin the next cycle Lesson Overview Energy and Life e. Summary of the Calvin Cycle i. 6 molecules of carbon dioxide are used to produce a single 6carbon sugar molecule ii. The energy for the reactions is supplied by compounds produced in the light-dependent reactions Lesson Overview Energy and Life Lesson Overview Energy and Life C. Factors Affecting Photosynthesis a. Temperature, Light, and Water i. Temperatures above or below 0-35 C may slow down the rate of photosynthesis or stop it entirely i. Shortage of water can slow or stop photosynthesis Lesson Overview Energy and Life iii. High light intensity increases the rate of photosynthesis iv. After reaching a certain level of light intensity, plant reaches its maximum rate of photosynthesis Lesson Overview Energy and Life i. C4 Photosynthesis 1. Have specialized chemical pathways that allow them to capture even very low levels of carbon dioxide and pass it to the Calvin cycle 2. Require extra energy in the form of ATP 3. Examples: corn and sugar cane Lesson Overview Energy and Life ii. CAM Plants 1. Admit air into their leaves only at night 2. During the daytime, leaves are tightly sealed to prevent water loss 3. Examples: cacti, succulents and pineapple trees