Can plants think? Do plants have a social life? Pre-syringa leaf c.s. Plants: Leaf Cross Section Plants review: Vascular Tissue: Xylem, transports water Phloem, transports food Gas intake: Guard cells open forming STOMATES Stomates and Guard cells PHOTOSYNTHESIS: The starting point of life* Experiment design Experiment design: Is light necessary for photosynthesis? Experiment….predict outcome BLUE, EMPTY, LIGHT EMPTY, LIGHT LIGHT DARK RESULTS: explain LIGHT LIGHT LIGHT DARK st 1 concept to know: Living things run on batteries. What is the battery? We recharge ATP from sugar (glucose) …where does sugar come from? Adenosine Triphosphate (ATP) Molecule that provides life’s energy. – Works by popping of a phosphate group, releasing stored energy – 3 parts: Adenine Ribose 3 Phosphate groups Figure 8-3 Comparison of ADP and ATP to a Battery Section 8-1 ADP ATP Energy Adenosine diphosphate (ADP) + Phosphate Partially charged battery Energy Adenosine triphosphate (ATP) Fully charged battery An organism can either make its own food, or eat it. They are called autotrophs or heterotrophs Nutrition Nutrition Autotrophic Nutrition Photosy nthesis Green Plants Chemosy nthesis Heterotrophic Nutrition Ingestion, Digestion, Egestion PHOTOSYNTHESIS: process which light energy is converted into sugar energy PBS interactive How do we know what goes in and out of plants? Major Players in the Development of the Photosynthesis Equation …how did they each contribute? What where their results? Conclusions? • Van Helmont: what ingredient makes up plants? • Priestly: What are plant byproducts? • Ingenhousz: Is light really that important? • What was his conclusion? • Of what was he ignorant? Priestly conclusion? Ingenhousz conclusion? Conclusion of scientists: formula WHY ARE PLANTS GREEN? The chloroplast: the plant 1º worker Chloroplast Anatomy The mechanics of photosynthesis Figure 8-7 Photosynthesis: An Overview Section 8-3 water CO2 Chloroplast Chloroplast NADP+ ADP + P LightDependent Reactions Calvin Cycle ATP NADPH O2 Sugars Photosynthesis + H2 O CO2 Energy Which splits water ATP and NADPH2 Light is Adsorbed By Chlorophyll ADP NADP Chloroplast O2 Light Reaction Calvin Cycle Used Energy and is recycled. + C6H12O6 Dark Reaction Resources: Light-Dependent Reactions Photosynthesis animation, VCAC Photosynthesis video, Mastering Biology Photosystem II Hydrogen Ion Movement Chloroplast ATP synthase Inner Thylakoid Space Thylakoid Membrane Stroma Electron Transport Chain Photosystem I ATP Formation Figure 8-11 Calvin Cycle Section 8-3 CO2 Enters the Cycle Energy Input ChloropIast 5-Carbon Molecules Regenerated 6-Carbon Sugar Produced Sugars and other compounds Comparison of Mitochondria and Chloroplasts Both have a large amount of internal membrane surface area. Both have their own ribosomes. Both have their own genomes. Both produce a large amount of ATP. Both derive energy for ATP synthesis from H+ pumps. The mitochondrial genome (in humans) is about 16,000 nucleotides long. The chloroplast genome is about 10x the size of the mitochondrial genome. Quick Quiz 1. What is the ultimate purpose of photosynthesis? 2. Where does the Calvin Cycle occur? 3. What is the purpose of water? 4. What is the purpose of light? 5. What is the primary raw ingredient of sugar? 6. Where do the Light-Dependent Reactions occur? 7. What makes the turbine spin that makes ATP? 8. What is the waste product of the Light-Dependent Reactions? 9. Why are plants green? Test yourself Section 8-3 includes use to produce takes place in take place in of uses to produce Do Now Section 8-3 Photosynthesis includes Lightdependent reactions use energy from sunlight Calvin cycle thylakoids to produce ATP NADPH takes place in take place in stroma of O2 Chloroplasts ATP uses NADPH to produce High-energy sugars Chromatography Lab follow-up Figure 8-5 Chlorophyll Light Absorption Section 8-2 Absorption of Light by Chlorophyll a and Chlorophyll b Chlorophyll b Chlorophyll a V B G YO R ENERGY ABSORPTION ON AN ATOMIC LEVEL Chlorophyll, a green pigment molecule, allows plants to absorb light energy. A pigment molecule obtains energy only at certain frequencies of light. Energy insufficient to reach an excited state is not absorbed (click 1). Similarly, energy that drives an electron past one energy level but is insufficient to reach a second is not absorbed (click 1). To be absorbed, the energy must be sufficient to reach only allowable energy states (click 1). This simple rule of quantum physics is all you need to know to understand an absorption spectra of chlorophyll (click 1). 2 Chlorophyll b Absorption Intensity excited states 1 ground state Chlorophyll a 300 400 500 600 700 http://seawifs.gsfc.nasa.gov/SEAWIFS.html