Chapter 8 Photosynthesis Ch. 8-1 Energy & Life • All living things need energy • Plants capture energy from sunlight to produce food (autotrophs) • Animals get energy from food (heterotrophs) Chemical Energy • One of the most imp. compounds that living things use to store energy – ATP • ATP – adenosine triphosphate • Has 3 parts: → • When the bond is broken b/t the 2nd & 3rd phosphate energy is released ATP – ADP cycle • When ATP gives up some energy, it’s converted to ADP (adenosine diphosphate) • ADP doesn’t have much energy, so it must be re-converted to ATP again Ch. 8-2 Investigating Photosynthesis • Van Helmont – discovered that plants gain most of their mass from water Investigating Photo • • Priestly – discovered oxygen ←his expt. Ingenhousz – showed that plants give off O2 only when exposed to light • Equation for Photosynthesis • 6 CO2 + 6 H2O → C6 H12 O6 + 6 O2 • carbon dioxide + water → glucose + oxygen Light & Photo • Plants gather sunlight with light-absorbing molecules called pigments *necessary for photosynthesis! • Main pigment is chlorophyll • Chlorophyll absorbs light only in certain parts of the visible spectrum • Two types of Chlorophyll: • Chlorophyll a - absorbs blue-violet and red light. • Chlorophyll b • - absorbs blue and red light. Green light is not absorbed! It’s reflected! Light & Photo • Plants do not absorb light well in the green part of the spectrum. Why? (green light is reflected by plants, which is why they appear green) • A pigment will be the color of the spectrum of light that it CANNOT absorb! http://www.sciencelearn.org.nz/ScienceStories/Harnessing-the-Sun/Sci-Media/Animations-and- Accessory Pigments: Their purpose is to absorb light where chlorophyll does not – they absorb green light! (They complete the absorption spectrum) • Carotenoids: which produce orange, red, and brown colors in such things as carrots, cranberries, grapes, and rutabagas • Anthocyanins: produce purple, blue and red colors, such as: concord grapes, blueberries, and plums. • Xanthophylls: produce yellow colors, such as in corn and daffodils stoma Ch. 8-3 The Reactions of Photo • Take place in the chloroplasts • Parts of a chloroplast: thylakoids: saclike photosynthetic membranes granum(a) – stacks of thylakoids stroma – space outside thylakoid http://www.mhhe.com/biosci/bio_animations/02_MH_P hotosynthesis_Web/ great review video Steps of Photosynthesis • Two main parts: 1. Light-dependent reaction 2. Light-independent reaction (Calvin Cycle) 6 CO2 + 6 H2O → C6 H12 O6 + 6 O2 (2) (1) (2) (1) Electron Carriers • When light excites electrons (e-) in chlorophyll, they gain energy • These high-energy e- require a special carrier • NADP+ is one e- carrier • It is like an uncharged battery (like ADP) • It turns to NADPH when charged • http://www.mhhe.com/biosci/bio_animations/02_MH_Photosynthesis_Web/ Light-Dependent Reaction • Take place in the grana / thylakoid membrane (with chlorophyll) • Raw materials: light, water, ADP & NADP+ (uncharged batteries) • Products: ATP, NADPH (charged batteries) & O2 (given off into air) http://www.sumanasinc.com/webcontent/an http://www.science.smith.edu/departme • Video Slower animation imations/content/harvestinglight.html Biology/Bio231/ltrxn.html Step 2 – Light Independent Reaction (Calvin Cycle) • Raw materials: ATP, NADPH & CO2 • Products: C6H12O6 • Takes place in the stroma Calvin Cycle – add to picture • Uses ATP and NADPH from the light reactions to produce high-energy sugars such as glucose. • Uses 6 molecules of CO2 to produce one 6-Carbon sugar. • The sugar is either then used for immediate energy or to build larger molecules such as cellulose or starch. • Calvin cycle video clip • http://highered.mcgrawhill.com/sites/0070960526/student_view0/ chapter5/animation_quiz_1.html • Calvin cycle animation • http://faculty.nl.edu/jste/calvin_cycle.htm Overview of Photosynthesis Factors Affecting Photosynthesis a. Amount of water b. Temperature (photo depends on enzymes that work best at 0°C to 35°C (32oF to 95oF) c. Light Intensity d. CO2 concentration e. surface area of leaves Factors Affecting Photo Photosynthesis Review Round 1 Number your paper 1-8. 1. Name the energy storage molecule (the charged battery)._______ 2. What are organisms called that can produce their own food?_______ 3. What molecule can be compared to a partially charged battery?________ 4. Name the ultimate source of energy for plants & animals._____ 5. What are organisms called that get energy from other animals & plants? ________ 6. What is the location of the high energy bond in ATP? _________ 7. What is the simple sugar that stores great amounts of energy? ________ 8. The 3 parts of ATP are: ________, _________ & 3 _________. Answers – Exchange papers with a partner Answers – Round 1 • • • • • • • • 1. 2. 3. 4. 5. 6. 7. 8. ATP Autotroph ADP The sun Heterotrophs Between the 2nd & 3rd phosphate Glucose Adenine, ribose, 3 phosphates Photosynthesis Review Round 2 • • 1. 2. Number your paper 1-4 Match each scientist with their contribution to photosynthesis: Plants gain most of their mass through water. Using a candle & a jar, he observed that plants produce a gas that kept the candle burning. 3. 4. Found that plants need sunlight to produce oxygen. Traced the path in plants that produces glucose; known as the light-independent reaction. Priestly Ingenhousz van Helmont Calvin Answers – Exchange papers with a partner Answers – Round 2 • • • • 1. 2. 3. 4. van Helmont Priestly Ingenhousz Calvin Photosynthesis Review Round 3 • Number your paper 1-9. • 1. Write the equation for photosynthesis in symbols. • 2. Write the equation for photosynthesis in words. • 3. Plants gather light with molecules called _______; the main one in plants is ____________. • 4. Two areas of the visible spectrum that chlorophyll absorbs light well from are ______ & ______. • 5. Photosynthesis occurs in the _________ of plants. • 6. Photosynthetic membranes in the chloroplasts are called ___________. • 7. A ______ is a stack of thylakoids. • 8. The _________ is the space outside the grana. • 9. _______ can be compared to a device used to transfer hot coals. • Answers – Exchange papers with a partner. Answers – Round 3 • 1. 6CO2 + 6H2O + light → C6H`2O6 + 6O2 • 2. carbon dioxide + water + light → glucose + oxygen • 3. pigment; chlorophyll • 4. blue & red • 5. chloroplasts • 6. thylakoids • 7. granum • 8. stroma • 9. NAPD+ Photosynthesis Review Round 4 Number your paper 1-9. 1. The part of photosynthesis that requires light is called the _____________ reaction. 2. The part of photosynthesis that does not require light is called the _______ cycle. 3. Name 4 raw materials of the light- dependent reaction: ______, ______, ______ & ______. 4. Name 3 products of the light-dependent reaction: ______, ______ & ______. 5. Name 3 raw materials of the Calvin cycle: _____, ______ & ________ 6. Name 1 product of the Calvin cycle: ________. 7. The light-dependent reaction takes place in the _______. 8. The Calvin cycle takes place in the ________. 9. Name 3 factors that affect the rate of photosynthesis: • Answers – Exchange papers with a partner. Answers – Round 4 1. 2. 3. 4. 5. 6. 7. 8. 9. light-dependent Calvin cycle (light-independent) Light, water, ADP & NADP+ ATP, NADPH, & O2 ATP, NADPH & CO2 C6H12O6 (glucose) grana stroma 9. temperature, light & water Photosynthesis Review Round 5 • 1. 2. 3. 4. Number your paper 1-8. Multiple Choice: Which of the following is an autotroph: A. cheetah B. mushroom C. fern D. frog One of the most important compounds that living things use to store energy is: A. DNA B. ATP C. H2O D. CO2 Which scientist discovered oxygen? A. Van Helmont B. Calvin C. Priestly D. Ingenhousz The products of photosynthesis are: A. sugar & oxygen B. water & CO2 C. oxygen & hydrogen D. sugar & carbon dioxide Photosynthesis Review Round 5 5. 6. 7. 8. Which substance from the light-dependent reaction of photosynthesis is a source of energy for the Calvin cycle? A. ADP B. H2O C. NADPH D. CO2 In which structures in a chloroplast does the Calvin cycle take place? A. stroma B. grana C. thylakoid D. stacks of pancakes The leaves of a plant appear green because chlorophyll: A. reflects blue light B. absorbs blue light C. reflects green light D. absorbs green light Which molecule stores the most amount of energy? A. ADP B. ATP C. fat D. glucose Answers – Round 5 1. C 2. B 3. C 4. A 5. C 6. A 7. C 8. D • Answers – Exchange papers with a partner