ENERGY IN THE CELL Usable Energy Energy is stored when bonds are created and released when bonds are broken. The molecule used by the cell to transport energy from one reaction to another is called ATP (adenine triphosphate) . Phosphate groups have a positive charge which means they are difficult to force together. By attaching three of them together the cell stores a lot of energy in the bond created between the 2nd and 3rd phosphate. Releasing Energy In order to use the energy stored in ATP (perhaps to put CO2 together into sugar) the bond between the 2nd and 3rd phosphate is broken and the 3rd phosphate is removed. When this occurs the molecule left with 2 phosphates is called ADP (adenine diphosphate) and has little energy stored. A Reusable Cycle When energy is released from ATP the ADP and single phosphate are not destroyed. ADP and the phosphate can be attached back together using the energy that comes out of an exergonic reaction. This stored energy can then be transported to any other reaction as an energy source. This makes ATP reusable (like a rechargeable battery) PHOTOSYNTHESIS The Big Picture The process of photosynthesis is carried out by plants and some unicellular organisms in order to use the energy from light to assemble sugars. This will transform the light energy into potential energy for the cell. This entire process will occur in the chloroplast. Step 1 the light dependent reaction occurs in the thylakoid. Step 2 the Calvin Cycle occurs in the stroma. During photosynthesis the cell will bring in light energy, water, and carbon dioxide and use these reactants to create oxygen and glucose (sugar). The chemical reaction is: Light + 6 H2O + 6 CO2 C6H12O6 + 6 O2 reactants products Step 1: Light-dependent Reaction Water is brought into the thylakoid and broken apart to remove electrons. The oxygen from the water is released as a byproduct. The electrons are put into a group of chlorophyll pigment called a photosystem. The photosystem collects light energy in order to energize the electron. The electron passes down a series of proteins imbedded in the membrane. As they travel down the electron transport chain (ETC) the electron’s energy is used to create ATP which is the usable energy for the cell. Step 1: Light-dependent Reaction The electrons will end up in a second photosystem where they receive more energy from light and are passed down another ETC. At the end of the 2nd ETC the electrons must be stored so they do not damage the cell. The electron carrier NADP+ will catch the electron becoming NADPH. Step 2: The Light Independent Reaction AKA The Calvin Cycle The energy in ATP and NADPH made in the first reaction will be used to put CO2 together to make sugar in the second reaction. Remember that creating bonds requires and stores energy. The cycle will start with a 5 carbon molecule called RuBP. Carbon dioxide is brought into the cell from the environment and attached to the RuBP. At this point the energy made in the first reaction is used to rearrange and break this molecule in half creating two 3 carbon molecules called PGAL. This will occur three times creating 6 PGAL. Step 2: The Light Independent Reaction AKA The Calvin Cycle At this point one of the PGALs is reserved while the remaining five are rearranged back into the three RuBPs that were used. The process repeats making 6 more PGAL. Again one is reserved while the other five are rearranged back into RuBP. The two PGALs that have been reserved can now be attached to create glucose. This process took six turns of the cycle. But, the RuBP was recreated so process is able to continue. Recap Photosynthesis occurs in the chloroplast of plants and some unicellular organisms. Photosynthesis uses light, water, and carbon dioxide to make oxygen and sugar. The chemical reaction is: Light + 6 H2O + 6 CO2 C6H12O6 + 6 O2 Step 1 The Light Dependent Reaction: Occurs in the thylakoid. Uses water and sunlight. Produces oxygen (which is released), ATP and NADPH (energy for the next cycle). Step 2 The Calvin Cycle: Occurs in the stroma. Uses ATP and NADPH breaking them into ADP and NADP+ to be sent back to the first reaction for more energy. Also uses CO2 and RuBP. Produces PGAL which is turned into glucose (sugar) and RuBP to be reused. Warm Up: Write and answer the questions on pg 32 What is the main function of photosynthesis? In what organelle does photosynthesis occur? What are the reactants of photosynthesis? What are the products? What can change the rate of photosynthesis and how would the rate change? What types of organisms carry out photosynthesis? plants Write out the reaction of photosynthesis. What is the main function of photosynthesis? Make sugar In what organelle does photosynthesis occur? chloroplast What are the reactants of photosynthesis? Light, water, and CO2 What are the products? Oxygen, sugar (glucose/starch) What can change the rate of photosynthesis and how would the rate change? Temperature: any temp other than optimum slows reaction Ph: any pH other than optimum slows reaction Amount of reactants: faster reaction with more reactants What types of organisms carry out photosynthesis? plants Write out the reaction of photosynthesis. CO2 + H2O + LIGHT C6H12O6 + O2 CELLULAR RESPIRATION The Big Picture The process of cellular respiration is carried out by all eukaryotic cells that can obtain oxygen to break down sugar in order to create the energy molecule ATP. The majority of this process occurs in the mitochondria. Step 1 glycolysis occurs in the cytoplasm. Srep 2 the Kreb’s cycle occurs in the mitochondria. Step 3 the electron transport chain is attached to the inner membrane of the mitochondria. During cell respiration the cell will bring in glucose (sugar) and oxygen and produce carbon dioxide, water, and ATP. The chemical reaction is: C6H12O6 + 6 O2 6 H2O + 6 CO2 + 36 ATP reactants products Step 1 Glycolysis Occurs in the cytoplasm. The bond in the middle of glucose is broken forming two 3 carbon molecules called pyruvic acid. Like most chemical reactions, glycolysis requires some activation energy in the form of 2 ATP to start the reaction. Once the bond is broken the electron that was being shared is placed in an electron carrier called NAD+ to make NADH. This way the electron’s energy can be used later. The energy that is released by the breaking bond is used to create 4 ATP molecules meaning this step makes 2 net ATP. Step 2 Kreb’s Cycle The pyruvic acid made in the first reaction is moved onto the mitochondria. As the pyruvic acid is moved one of the carbons is broken off and released as CO2. The breaking of this bond releases another electron which is placed on NAD+ making NADH to be used later. The energy that was released by the bond breaking is used to form a new bond between the remaining two carbons and a coenzyme A to form AcetylCoA. Step 2 Kreb’s Cycle Once Acetyl-CoA is made the molecule has the correct shape to react with the enzymes in the next part. Through a series of chemical reactions the Acetyl-CoA is broken down. The remaining two carbons are released as CO2. The electrons from each broken bond are placed into the electron carriers NADH and FADH2 so that their energy can be used later. The energy released from the breaking of the bonds is used to create 1 ATP. Remember that there were 2 pyruvic acids so this process occurs twice for a total of 2 ATP. Step 3 Electron Transport Chain The electron carriers NADH and FADH2 made in the first steps are moved to the inner membrane of the mitochondria. The electrons are removed and passed down a series of proteins releasing a little bit of energy at a time. The energy released is used to create 32 ATP. At the end of the ETC the electrons are used to create bonds between oxygen and hydrogen to make water. Recap Cell respiration occurs mostly in the mitochondria of almost all eukaryotic cells. Cell respiration breaks down sugar using oxygen to create water, carbon dioxide, and 36 ATP molecules. The chemical reaction is: C6H12O6 + 6 O2 6 H2O + 6 CO2 + 36 ATP Step 1 Glycolysis: occurs in the cytoplasm Sugar is broken in half using 2 ATP. This creates pyruvic acid, 4 ATP and NADH. Step 2 Kreb’s Cycle: occurs in the mitochondria Pyruvic acid is broken down and released as CO2. The electron carriers NADH and FADH2 as well as 2 ATP are also produced. Step 3 ETC: occurs on the inner membrane of the mitochondria The electrons in NADH and FADH2 are placed into a series of proteins where the energy is released a little at a time and used to create 32 ATP. The electrons are then used to attach oxygen and hydrogen together to make water. Warm Up: write and answer the questions on pg 32 What is the main function of cell respiration? In what organelle does cell respiration occur? What are the reactants of cell respiration? What are the products? What can change the rate of cell respiration and how would the rate change? What types of organisms carry out cell respiration? Write out the reaction of cell respiration. What is the main function of cell respiration? Use oxygen to break down sugar to make ATP energy In what organelle does cell respiration occur? mitochondria What are the reactants of cell respiration? Glucose/sugar and oxygen What are the products? Water, CO2, and 36ATP What can change the rate of cell respiration and how would the rate change? Temperature: any temp other than optimum slows reaction Ph: any pH other than optimum slows reaction Amount of reactants: faster reaction with more reactants What types of organisms carry out cell respiration? All eukaryotic cells (animals AND plants) Write out the reaction of cell respiration C6H12O6 + O2 CO2 + H2O + 36ATP FERMENTATION The Big Picture Fermentation is a process used to break down sugar without oxygen. There are situations (such as sprinting) in which the cell does not have enough oxygen to break down sugar through cell respiration. Some smaller organisms never carry out cell respiration because they do not require that much energy and the alternative is faster. While fermentation is faster it only creates 2 ATP from a sugar molecule. Therefore it is not as efficient as cell respiration which created 36 ATP for each sugar molecule. There are two kinds of fermentation but each organism only carries out one or the other. Alcoholic Fermentation Occurs in the cytoplasm. Step 1: Glycolysis The bond in the middle of glucose is broken forming two 3 carbon molecules called pyruvic acid. This requires 2 ATP, but it makes 4 ATP and NADH Step 2: The electron stored in NADH is used to break off a carbon as CO2 and rearrange the remaining two carbons into ethyl alcohol. Yeast and bacteria are the two most common organisms to carry out alcoholic fermentation. We use fermenting yeast to make CO2 bubbles causing bread to rise. Lactic Acid Fermentation Occurs in the cytoplasm. Step 1: Glycolysis The bond in the middle of glucose is broken forming two 3 carbon molecules called pyruvic acid. This requires 2 ATP, but it makes 4 ATP and NADH Step 2: The electron stored in NADH is used to rearrange pyruvic acid into lactic acid. Human muscle cells will carry out this type of fermentation for quick energy or when there is not enough oxygen to meet the energy demands such as during a sprint.