Photosynthesis and Cellular Respiration Why are oxygen bubbles a good ? indication of photo Oxygen is produced as a waster products when H2O is broken down by photons. It leaves out of the stomatas. You can see the rate of photosynthesis by counting the bubbles that rise in the water. More bubbles more oxygen produced more photosynthesis. Why is there more photosynthesis with white than green? Pigments in plants do not use green light. They reflected it so with green light there is little photosynthesis. White light is all colors mixed together so there will be a variety of wavelengths available for the pigments. Why is it better that there is more pigments than one? When there are multiple pigments, then the plant an absorb can use multiple wavelengths. If they only had chlorophyll a then thy would only absorb the extreme of violents and reds. By having chlorophyll b and carentoids, pigments can absorb other blues oranges, and yellows. This allows the plant to absorb many more wavelengths. Why do the leaves change color in the fall? Chlorophyll a and b reflect green and are the dominant pigments. In the fall, dissolve first leaving acessory pigments such as the carentoids which reflecy reds and oranges. SO the leaves will lose their green color first so that we can see the carentoids. Purpose of Photosynthesis GLUCOSE!!!!!!!!! What Do Plants Need to Complete Photosynthesis? How Do the Materials Enter the Plant? We must learn the different parts of the cell that is important first!!!! Light Energy Harvested by Plants & Other Photosynthetic Autotrophs 6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2 THE BASICS OF PHOTOSYNTHESIS • Almost all plants are photosynthetic autotrophs, as are some bacteria and protists – Autotrophs generate their own organic matter through photosynthesis – Sunlight energy is transformed to energy stored in the form of chemical bonds (c) Euglena (b) Kelp (a) Mosses, ferns, and flowering plants (d) Cyanobacteria Parts of the Leaf!!! Parts of Plant Important to Photosynthesis Parts of the Leaf Mesophyll - middle layer of leaf where chloroplast are concentrated. Stomata - pores on bottom of leaf where carbon dioxide enters and oxygen leaves. Chloroplasts: Organelles that contain pigments which absorbs light. What Parts do You Know? Stomata (stoma) Pores in a plant’s cuticle through which water and gases are exchanged between the plant and the atmosphere. Oxygen (O2) Carbon Dioxide (CO2) Guard Cell Guard Cell Stomata Guard Cells open and close depending on vacuoles!!!! Mesophyll Cell Nucleus Cell Wall Chloroplast Central Vacuole Mesophyll Chloroplast Organelle where photosynthesis takes place. Stroma Outer Membrane Inner Membrane Thylakoid Granum Chloroplasts Parts Stroma - Thick fluid around grana where sugars are made in Calvin Cycle Thylakoid - membrane sacs that contains pigments and enzymes Grana - Stacks of thylakoids Occurs in the membrane of the thylakoids Thylakoid Thylakoid Membrane Granum Thylakoid Space The location and structure of chloroplasts Chloroplast LEAF CROSS SECTION MESOPHYLL CELL LEAF Mesophyll CHLOROPLAST Intermembrane space Outer membrane Granum Grana Stroma Inner membrane Stroma Thylakoid Thylakoid compartment Other Important Parts Xylem – vessels that carry water from roots to leaves for photosynthesis. Phloem – vessels that carry sugars from the leaves to other parts of the plant. WHY ARE PLANTS GREEN? Plant Cells have Green Chloroplasts The thylakoid membrane of the chloroplast is impregnated with photosynthetic pigments (i.e., chlorophylls, carotenoids). THE SUN: WHY IS IT IMPORTANT? Source of light energy Source of heat energy Gravitational attraction Source of radiation Day and night Source of all energy(electricity) Source of food for all organisms!!!! What part of the spectrum do we see? Visible light – 380 - 750 Sun’s Electromagnetic Spectrum The Sun’s energy travels to Earth in waves. Wavelength - Distance between 2 crest of waves. Shorter the wave the more energy it contains. UV waves - short waves that damage organic tissue(cancer). Electromagnetic Spectrum and Visible Light Gamma rays X-rays UV Infrared & Microwaves Visible light Wavelength (nm) Radio waves SUN’S SPECTRUM WHY ARE PLANTS GREEN? Different wavelengths of visible light are seen by the human eye as different colors. Gamma rays X-rays UV Infrared Visible light Wavelength (nm) Microwaves Radio waves THE COLOR OF LIGHT SEEN IS THE COLOR NOT ABSORBED Chloroplasts absorb light energy and convert it to chemical energy They absorb all colors but green Light Reflected light Transmitted light Chloroplast Absorbed light The feathers of male cardinals are loaded with carotenoid pigments. These pigments absorb some wavelengths of light and reflect others. Sunlight minus absorbed wavelengths or colors equals the apparent color of an object. Why are plants green? Transmitted light Pigments Pigments - light-absorbing molecules located in the thylakoid membranes of chloroplast. They absorb some light and reflect the others. Which would we see? Reflected!!! Chlorophyll Molecules- IT IS A PIGMENT!!! Located in the thylakoid membranes. Chlorophyll pigments harvest energy (photons) by absorbing certain wavelengths (blue-420 nm and red-660 nm are most important). Plants are green because the green wavelength is reflected, not absorbed. Why are Chloroplast Important? The chloroplasts absorb the Sun’s energy and use this energy to excite electrons which powers photosynthesis. To break apart water and carbon dioxide, you must have energy!!!! Why is it important the plants have multiple pigments Broaden the amount of photons collected for photosynthesis!!! 3 Types of Pigments in Thylakoid Chlorophyll a - Participates in light reaction by absorbing blue-violet and red light. Chlorophyll b - absorbs blue and orange light. Carenotiod - absorbs blue and reflects reds, yellow, and orange. Chlorophyll a - MAIN PIGMENT Chlorophyll a: This is the most abundant pigment in plants and main pigment of photosynthesis. Chlorophyll a absorbs light with wavelengths of 430nm(blue) and 662nm(red). It reflects green light strongly so it appears green to us. Accessory Pigments THEY assist chlorophyll a by passing photons to it!! 1. Chlorophyll b - absorbs blue and orange light. 2. Cartenoids - absorbs blue and reflects reds, yellow, and orange. 3. Xanthophyll: absorb blue reflect red and yellow Chorophyll b This molecule has a structure similar to that of chlorophyll a. It absorbs blue and orange light of 453nm and 642 nm maximally. It is not as abundant as chlorophyll a, and probably evolved later. It helps increase the range of light a plant can use for energy. 2. Cartenoids - Beta Carotene This is a class of accessory pigments that occur in all photosynthetic organisms. Carotenoids absorb some green light 460 nm and 550 nm and appear brown, red, orange, or yellow to us. 3. Xanthophylls Xanthophylls are a fourth common class of pigments. They are usually red and yellow and do not absorb energy as well as cartenoids. Why Would It Be Helpful to Have More Than One Pigment? If there are more than one pigment, then it broadens the amount of pigments that the plant can use. This is why leaves can be different colors. Different pigments absorb light differently Why do the leaves change color in winter? Chlorophyll a and b break down first before the carentoids. So they reflect the oranges, reds, and yellows!!!! Why is Sunlight Needed for Photosynthesis? It contains photons(energy) that are trapped by chlorophyll(pigments) from visible light!!!! It absorbs the light to use as energy. Reflected not used!!! Energy is the ability to do work! Cells must have the energy to continue on with cell processes and produce heat. ATP – source of cell energy Autotrophs – produce carbohydrates Plants, algae, blue-green bacteria, and some bacteria are autotrophs 10% of Earth’s species are autotrophs. Heterotrophs – consumer Fungi, animals, some bacteria and some Photosynthesis and Cellular Respiration Processes involved in converting sunlight into ATP. Celllular respiration is conducted by ALLLL organisms!!!! ATP – energy Stored in 3rd phospate Photosynthesis is to make glucose Photosystems Photosystems: Clusters of proteins and pigments that trap the Suns energy called photons. This is where chlorophyll is located. Located in the thylakoid membranes. The photosystems trap a photon and this excited an electron to a higher level of energy. Photosystems These structures are the main structure that traps the Suns energy so that the H2O can be separated. Mixture of pigments and proteins Photosystem Photosystem I and II are proteins and pigments to trap the Sun’s photons. Located in the thylakoid memebrane!!! Photosystem Important Molecules in Photosynthesis NADP and ADP are energy carriers!! They carry the electrons which is energy!!! 2 Steps of Photosynthesis 1.Light dependent reactions: Thylakoid membranes. Pigments trap photons H2O is split 2. Calvin Cycle: Occurs in stroma. CO2 is used to make glucose Overview of Photosynthesis Step 1 – Light dependent reaction(depends on Light) Traps the sunlight and energy is moved along the thylakoid membrane. Water is broken in to O and H by the electrons tha are in ATP and NADPH required for dark reaction. Oxygen given off as waste. Photosystem I and photosystem II - pigments Step 2 – Dark reaction(Calvin Cycle - stroma Carbon Dioxide now is added to cycle to build glucose.Uses ATP and electrons from light reaction to make glucose. Describe what is happening the diagram. Photosynthesis Step 1 – Light Dependent Reaction The light reactions convert solar energy to chemical energy. Takes place in the thylakoid membrane. Photosystem II and electron transport 1. Pigments in photosystem II absorbs the sun’s energy to break apart water into H, O and electrons. 2. These high energy electrons are passed along the electron transport chain to photosystem I. 3. H+ ions are passed to ATP synthase to make ATP. NADPH is made in photosystem I. 4. Oxygen is a waste product leaves by stomata. Electron Transport Chain Series of electron carrier proteins that shuttle high energy electrons. High - energy electrons move down the ETC to photosystem I where it is used to pump H ions across the thylakoid membrane and into the thylakoid space. Photosynthesis Photosystem I 1. Electrons from photosytem II is moved along the membrane to photosystem I. 2. Electrons are added to NADPH which is the energy carrier for the rest of photosynthesis. 3. The H ions are pumped through a protein channel as part of an enzyme ATP synthase to make ATP. Summary of Light-dependent Reaction * Energy is captured from sunlight and transferred to electrons(electron transport chain). Water molecule pulled apart to provide H ions. The ions are used to make ATP and NADPH. Reactants: sunlight and water Products: ATP and NADPH which will be the energy for the Calvin Cycle!!! Why is water needed? Water is split to replace the electrons that are used in the pigments. As water is split, it replaces the electrons. When H and split from O, it releases electrons and O as a waste. Plants produce O2 gas by splitting H2O The O2 liberated by photosynthesis is made from the oxygen in water (H+ and e-) Why do we need high energy electrons? To make ATP and NADPH which are the energy carriers. They are needed in the Calvin Cycle to make sugars. Thylakoid compartm ent (high H+) The production of ATP Lig ht Lig ht Thylakoid membrane Antenn a molecul es Stroma (low H+) ELECTRON TRANSPORT CHAIN PHOTOSYSTEM PHOTOSYSTE II MI ATP SYNTHASE Summary—Light Dependent Reactions a. Reactants light energy, H2O. b. Products ATP, NADPH, O2. Light Independent Reaction Calvin Cycle During the light - independent reaction, ATP and NADPH from the light dependent reactions are used to make high energy sugars. Light Independent Reaction Overview 1. Carbon dioxide added:Carbon Dioxide enters the plant from the atmosphere. Bonds with a 5-carbon sugar. 2. Three-carbon molecules formed: ATP and NADPH use enzymes in the stroma to split the six carbon into 3 carbon sugars. 3. Three-carbon molecules exit: Most 3 carbon stay in cycle. When 2 leave, they form glucose. 4. Three-carbon molecules recycled: Energy from ATP Change 3carbon molecules back into 5 carbon to start the cycle over again. *Energy provided by Light dependent reaction. The plants uses the carbohydrates to meet its energy needs to make all of the macromolecules that it needs(proteins, lipi Calvin Cycle 1. Carbon Dioxide is split and C is added to a 5 carbon sugar by an enzyme in the stroma of the chloroplast to make a 6 carbon sugar. 2.The 6 carbon sugar is divided into 2 by the ATP and NADPH from LDR. 3. One three carbon will leave and others stay in cycle. 4.After 2 cycles, glucose will be formed. 5.ATP is used to change the 3 carbon back to 5. Overview Calvin Cycle Reactants: ATP, NADPH, and Carbon dioxide Products: GLUCOSE!! The end goal – Make glucose from the SUN!! AN OVERVIEW OF PHOTOSYNTHESIS • Step 2 – Light Independent Reaction – CALVIN CYCLE Occurs in the stroma. • The Calvin cycle makes sugar from carbon dioxide 1.ATP generated by the light reactions provides the energy for sugar synthesis 2.The NADPH produced by the light reactions provides the electrons for the reduction of carbon dioxide to glucose. Carbon Dioxide is built to make a 6 carbon sugar called glucose. – END GOAL – to break carbon dioxide down and combine into glucose!!! Need energy to do this!! That is why ATP and NADPH was made!! Factors that affect Photosynthesis 1. Temperature: Enzymes will denature if do not stay in 0 -35 degree range. 2. Light intensity: too little light less photosynthesis but there is a max output they will accomplish. 3. Water: Water is needed for photosynthesis. No water no photosynthesis!!! Label Diagram Below Explain the role pigments play in photosynthesis. Describe 3 factors that would affect photosynthesis. Label picture below Complete the chart below Two Main Processes Reactants Products Location Carbon Oxygen Cycle The Purpose of Cellular Respiration Break down glucose into ATP!!!! You end up with ATP, H ions and electrons. The electrons are sent to the Electron Transport Chain where they help to make ATP through ATP synthase. Overall Equation for Cellular Respiration C6H12O6 + 6O2 YIELDS 6CO2 + 6H20 + e- + 36-38ATP’s Copyright Cmassengale Why so Many Steps? Energy is released slowly. If released all at once would be released as heat!!! GOAL: TO MAKE ATP, NADH, FADH2 to send them to the electron transport chain. They pump H+ across membrane(active transport). The H+ move through ATP synthase to make ATP. Cellular Respiration Breaking down sugars in presence of oxygen to make ATP!!!! ALL ORGANISM NEED ENERGY ALL ORGANISMS DO CELLULAR RESPIRATION!!! 2 Types of Respiration Glycolysis – 2 ATPS Anaerobic Aerobic No O O2 1. Lactic Acid 1.Transitional 2. Fermentation 2. Kreb Cycle 3. ETC 36 ATPS GOAL!!!!!! 4 Stages of Aerobic Respiration Glycolysis: before cellular respiration Occurs in the cytoplasm Glucose is broken down CELLULAR RESPIRATION 1. TRANSITIONAL –pyruvate to Acetyl Co-A 2. Krebs Cycle Breaks down pyruvate into CO2 Occurs in mitochondrial matrix 3. Electron Transport Chain ATP is synthesized - Occurs in mito membrane 1. Mitochondria Structure Smooth outer Membrane 2. Folded inner membrane 3. Cristae: Folds 4. Matrix: Space inside cristae Copyright Cmassengale Where Does Cellular Respiration Take Place? It actually takes place in two parts of the cell: Glycolysis occurs in the Cytoplasm Krebs Cycle & ETC Take place in the MitochondriaCopyright Cmassengale Net profit of ATP’s!!! 38 ATPS – glycolysis and cellular respiration!! Energy Carriers in CR *ATP *NAD *FAD NADP vs. NAD * Photosynthesis use the electron carrier - NADP (nicotinmide adenine dinucleotide phosophate) * Cellular respiration uses - NAD ( nicotinmide adenine dinucleotide) Are There Any Other Electron Carriers? YES! Another Coenzyme! FAD+ (Flavin adenine dinucleotide) Reduced to FADH2 Copyright Cmassengale Diagram of the Process Occurs across Cristae Occurs in Cytoplasm Occurs in Matrix Copyright Cmassengale Glycolysis(sugar splitting) Summary 1. Takes place in the Cytoplasm 2. Anaerobic (Doesn’t Use Oxygen) 3. Requires input of 2 ATP 4. Glucose split into two molecules of Pyruvate or Pyruvic Acid Copyright Cmassengale Glycolysis Glyco = glucose Lysis = break down LOCATION: Occurs in the cytoplasm This stage occurs in BOTH aerobic and anaerobic respiration Glucose breaks down into 2 pyruvate (2 ATP are also made) ◦ Glucose is a 6-carbon sugar ◦ Pyruvate is a 3-carbon molecule (there are two of them) Steps of Glycolysis 1.Two ATP molecules are used to energize a glucose molecule. 2. Glucose is split into 2 - 3 carbon molecules. Enzymes rearrange the molecules. 3. Electrons are transferred to NADP. The carbon molecules are converted to pyurate which enters cellular respiration. Glycolysis: Step 1 Glucose Figure 9–3 Glycolysis 2 Pyruvic acid To the electron transport chain Glycolysis Reactants and Products Reactants 1 glucose Enzymes are needed 2 ATP are needed to start Products 2 Pyruvates (go to next step) 4 ATP (2 are gained) 2 NADH (go to ETC) Really 10 steps with 10 different enzymes involved. TRANSITION REACTION Pyuvic Acid is shuttled into the mitochondria matrix where it is changes into Acetyl Co A. Acetyl CoA – this is the molecule that is used in Kreb Cycle!!! Cellular Respiration Overview After glycolysis, life diverges into two forms and two pathways 1. Anaerobic cellular respiration (aka fermentation) No oxygen 2. Aerobic cellular respiration I Oxygen needed!! ANAEROBIC VS. AEROBIC Anaerobic – no oxygen present fermentation or lactic acid can be formed. No oxygen then no cellular respiration. Aerobic –oxygen present. If oxygen is present , then cellular respiration can occur. Aerobic vs. Anaerobic Anaerobic DOES NOT require oxygen fermentation ◦ Simple ◦ fast ◦ produces smaller amounts of energy (ATP) Aerobic requires oxygen – cellular respiration ◦ Yields large amounts of energy ◦ What is this energy molecule? ATP, ATP, ATP Krebs Cycle Reactants and Products Reactants 2 Acetyl CoA NADH FADH Remember when you form a bond energy is released!! This is the key!! Products 2 ATP 6 NADH (go to ETC) 2 FADH2 (go to ETC) 4 CO2 (given off as waste) END GOAL – Make NADH and FADH NADH and FADH is carried to the elestron transport chain to make ATP. THE END OF GOAL!!! Main Goals of Krebs Cycle or Citric Acid Cycle Transfer high energy electrons(NADH and FADH) to molecules that can carry them to the electron transport chain to make 34 ATPS. Krebs Cycle Summary A. Requires Oxygen (Aerobic) B. Cycle series of oxidation(uses) reactions that give off CO2 and produce one ATP per cycle C.Turns twice per glucose molecule produces two ATP D. Location: matrix of mitochondria Copyright Cmassengale Section 9-2 Citric Acid Production Kreb Cycle 1. Coenzyme A enter the cycle and bonds to 4 carbon molecule 2. Citric Acid formed which is a 6 carbon. 3. Citric Acid broken down: into 5 carbon sugar carbon dioxide and NADH 4. 5 carbon sugar broken down: Into 4 carbon sugar, NADH, ATP and Carbon dioxide. 5. 4 carbon rearranged by enzymes. Molecules of NADH, FADH are formed to be carried down ETC. 6. 4 carbon molecule is recycled. Products of Kreb Cycle High energy carriers – NADH and FADH – This is the main goal!!! HYDROGEN IONS ARE SENT DOWN THE ELECTRON TRANSPORT CHAIN to make ATP. A Little Krebs Cycle History Copyright Cmassengale Discovered by Hans Krebs in 1937 He received the Nobel Prize in physiology or medicine in 1953 for his discovery Forced to leave Germany prior to WWII because he was Jewish Krebs Cycle ATP synthesis Electron Transport Electron Transport Chain Summary 34 ATP Produced H2O Produced Occurs Across Inner Mitochondrial membrane - Cristae Uses coenzymes NAD+ and FAD+ to accept e- from glucose NADH = 3 ATP’s FADH2 = 2 ATP’s Copyright Cmassengale GOAL OF ETC Remove the H from energy carriers and pump them across membrane for diffusion through ATP synthase to make ATP. Make 34 ATP form one glucose!!! In ETC 2 in Kreb Cycle 2 in Glycolysis =ATPS total 38 ATPS Electron Transport Chain Where inner membrane of mitochondria called cristea. Energy Yield Total of 32 ATP O2 combines with TWO H+ to form H2O Exhale - CO2, H2O comes from cellular respiration Electron Transport - Step 3 1. Proteins inside the membrane of the mito. Remove electrons from NADPh and FADH. 2. Electrons(hydrogen) are transported down the chain of the membrane to be pumped across. 3. ATP synthase(enzyme) puts a P on ADP to make ATP(END GOAL!!). 4. Oxygen enters the cycle to pick up electrons and hydrogen ions to make water that leaves the cycle. Electron Transport Chain Section 9-2 Electron Transport Hydrogen Ion Movement Channel Mitochondrion Intermembrane Space ATP synthase Inner Membrane Matrix ATP Production Electron Transport Chain Electron carriers loaded with electrons and protons from the Kreb’s cycle move to this chain-like a series of steps (staircase). As electrons drop down stairs, energy released to form a total of 32 ATP – Final Goal!! Oxygen waits at bottom of staircase, picks up electrons and protons and in doing so becomes water Electron Transport Chain Occurs in the cristae of the mitochondria Review of Mitochondria Structure Smooth outer Membrane l Folded inner membrane l Folds called Cristae l Space inside cristae called the Matrix l Copyright Cmassengale Diagram of the Process Occurs across Cristae Occurs in Cytoplasm Occurs in Matrix Copyright Cmassengale Energy Tally 36 ATP for aerobic vs. 2 ATP for anaerobic ◦ Glycolysis 2 ATP ◦ Kreb’s 2 ATP ◦ Electron Transport 32 ATP 36 ATP Anaerobic organisms can’t be too energetic but are important for global recycling of carbon Photosynthesis What happens to the glucose formed in photosynthesis? PHOTOSYNTHESIS CELLULOSE LIPIDS GLUCOSE respiration ATP Required to make plant cell walls. It is made of 100s of glucose molecules bonded together. Glucose is chemically converted to fatty acids and glycerol to make lipids, which are needed to make plant cell membranes and seed storage oils. STARCH Is used by roots and leaves to store excess glucose in an osmotically inactive form. It is made of 100s of glucose molecules. PROTEINS Using nitrate ions absorbed by plant roots, glucose is converted first to amino acids then to protein. CARBON DIOXIDE AND WATER The carbon dioxide can be used again in photosynthesis or may diffuse out of the leaf via the stomata Anaerobic Cellular Respiration When you exercise, you muscle to run out of oxygen to and produce lactic acid!! Some organisms thrive in environments with little or no oxygen ◦ Marshes, bogs, gut of animals, sewage treatment ponds No oxygen used= ‘an’aerobic What do they cells do withoutoxygen??? Cellular Respiration Overview After glycolysis, life diverges into two forms and two pathways 1. Anaerobic cellular respiration (aka fermentation) No oxygen 2. Aerobic cellular respiration I Oxygen needed!! Aerobic vs. Anaerobic Anaerobic DOES NOT require oxygen fermentation ◦ Simple ◦ fast ◦ produces smaller amounts of energy (ATP) Aerobic requires oxygen – cellular respiration ◦ Yields large amounts of energy ◦ What is this energy molecule? ATP, ATP, ATP Fermentation In absence of oxygen, fermentation releases energy from food molecules by producing ATP. Very small amounts of ATP!!! Fermentation Glycolysis occurs to produce ATP and NADH!! Normal Glucose is broken down into pyruvic acid to make ATP and NADH. This is the only ATP made. Two Types of Fermentation Alcoholic Fermentation Pyruvate converted to ethyl alcohol and CO2 Carried out by yeast and some bacteria Used in producing alcohol (both consumable and for ethanol), and for baking Lactic Acid Fermentation Pyruvate converted to lactic acid Carried out by muscles when working hard (muscles need ATP but can’t get O2 ) Causes muscle soreness and cramps Alcohol Fermentation Pyruvate Fermentation Occurs when O2 NOT present (anaerobic) Called Lactic Acid fermentation in muscle cells (makes muscles tired) Called Alcoholic fermentation in yeast (produces ethanol) Nets only 2 ATP Copyright Cmassengale Importance of Fermentation Alcohol Industry - almost every society has a fermented beverage. Baking Industry - many breads use yeast to provide bubbles to raise the dough. Alcoholic Fermentation Bacteria and fungi (yeast) Ethyl alcohol and carbon dioxide are the end products Process used to form beer, wine, and other alcoholic beverages Also used to raise dough, bread Lactic Acid Fermentation Uses only Glycolysis. Does NOT require O2 Produces ATP when O2 is not available. Lactic Acid Fermentation Carried out by human muscle cells under oxygen debt. Lactic Acid is a toxin and causes fatigue, soreness and stiffness in muscles. Lactic Acid Formation pyruvate + NADH----- lactic acid + NAD+ Lactic Acid Fermentation Glycolysis 4 ATP’s are produced Pyruvic Acid (3C) Lactic Acid (3C) Pyruvic Acid (3C) Lactic Acid (3C) Glucose (6 carbons) 2 ATP’s supply the activation energy 2 NAD+ + 2 e- 2 NADH 2 NAD+ + 2 e- 4 ATP Yield = 2 ATP Net Gain Fermentation - Summary Releases 2 ATP from the breakdown of a glucose molecule Provides ATP to a cell even when O2 is absent.