Cellular Structure and Function Chapter 7 Section 1: Section 2: Section 3: Section 4: Cell Discovery and Theory The Plasma Membrane Structures and Organelles Cellular Transport Section 1 Section 1: Cell Discovery and Theory Section 1: Cell Discovery and Theory The invention of the microscope led to the discovery of cells. K What I Know W What I Want to Find Out L What I Learned The history of Cell Theory http://ed.ted.com/lessons/thewacky-history-of-cell-theory#watch History of the Cell Theory • A cell is the basic structural and functional unit of all living organisms. • Cells are so small that their existence was unknown before the invention of microscopes. Cell Discovery and Theory History of the Cell Theory The cell theory • The cell theory is one of the fundamental ideas of modern biology, and includes the following three principles: • All living organisms are composed of one of more cells. • Cells are the basic unit of structure and organization of all living organisms. • Cells arise only from previously existing cells, with cells passing copies of their genetic material on to their daughter cells. Cell Discovery and Theory Discovery of Cells • Cells are about 1/500th the size of a . • In 1665 Robert Hooke used a 3-lens compound microscope to examine thin slices of cork. • He observed that cork is made of tiny, hollow compartments. • He gave them the name “cells” • Hooke was only looking at cell walls & empty space. Microscope Technology Compound light microscopes • Use a series of glass lenses and visible light to magnify images • Magnify images up to ~1000× actual size Electron microscopes • Create an image by illuminating a sample with a beam of electrons and collecting the electrons that are reflected back from the sample • Magnify images up to 500,000× actual size Cell Discovery and Theory Basic Cell Types • All cells have at least one physical trait in common: a plasma membrane. • A plasma membrane is a special boundary that helps control what enters and leaves the cell. • Most cells contain genetic material in some form. • Most cells break down molecules to generate energy. • There are two basic kinds of cells: eukaryotic and prokaryotic. Cell Discovery and Theory ALL ORGANISMS ARE MADE OF CELLS 1. ___________________________________________________________ THE CELL IS THE MOST BASIC UNIT OF LIFE. 2. ___________________________________________________________ ALL EXISTING CELLS ARE PRODUCED BY OTHER LIVING CELLS 3. ___________________________________________________________ HOOKE _____________ CORK The first to IDENTIFYcells. ____________ Responsible for NAMING them ____________ LEEUWENHOEK _____________ ANIMALCULES Made better LENSES ______________ and observed cells in greater DETAIL ______________. First to observe NUCLEUS ______________ SCHLEIDEN _____________ SCHWANN _____________ VIRCHOW _____________ RESPONSIBLE FOR CELL THEORY The first to note that _____________ PLANTS were made up of CELLS ___________ Concluded that all LIVING THINGS ___________ ___________ were made up of CELLS ___________ Proposed that all cells come from OTHER CELLS ____________ __________ Basic Cell Types • Eukaryotic cells contain a nucleus and other organelles. • Organelles are specialized structures that perform specific cell functions. • The nucleus is the distinct central organelle that contains the cell’s genetic material. • Eukaryotic cells are generally much larger than prokaryotic cells. Cell Discovery and Theory Basic Cell Types • Prokaryotic cells are cells without a nucleus or other membrane-bound organelles. • Smaller and simpler than eukaryotes • Probably similar to first organisms that lived on earth Cell Discovery and Theory Basic Cell Types Origin of cell diversity • Eukaryotic cells may have evolved from prokaryotic cells. • The endosymbiont theory proposes that a symbiotic relationship formed between two prokaryotic cells, one of which lived inside the other. • Eventually the symbiotic relationship led to the two cells becoming one. • Because eukaryotic cells are larger and more complex, they developed specific functions. • These specific functions led to cell diversity, and thus organismal diversity. Cell Discovery and Theory Eukaryotes VS. Prokaryotes Video • Prokaryotes VS. Eukaryotes Essential Questions • How are the advances in microscope technology related to discoveries about cells? • What are the similarities and differences between compound light microscopes and electron microscopes? • What are the principles of the cell theory? • What are the differences between a prokaryotic cell and a eukaryotic cell? Cell Discovery and Theory Section 2 Section 2: The Plasma Membrane Section 2: The Plasma Membrane The plasma membrane helps to maintain a cell’s homeostasis. K What I Know W What I Want to Find Out L What I Learned The Cell Membrane http://ed.ted.com/lessons/insightsinto-cell-membranes-via-dishdetergent-ethan-perlstein#watc Function of the Plasma Membrane • The process of maintaining balance in an organism’s internal environment is called homeostasis. • One of the structures responsible for homeostasis is the plasma membrane. • The plasma membrane forms a thin, flexible boundary between a cell and its environment. The Plasma Membrane Function of the Plasma Membrane • Plasma membranes have selective permeability, meaning they allow some substances to pass through while keeping others out. • Control of how, when, and how much of various substances enter and leave a cell depends on the structure of the plasma membrane. The Plasma Membrane Video • Selective Permeability • Selective Permeability 2 Structure of the Plasma Membrane The phospholipid bilayer • The plasma membrane is composed of phospholipid bilayer – two layers of phospholipids are arranged to allow the membrane to exist in a watery environment. • The phospholipid bilayer has polar heads facing outside and nonpolar tails facing inside the cell, allowing the membrane to keep the internal and external environments separate. The Plasma Membrane Phospholipids Video • Cell Membrane & Phospholipid Bilayer Video • Phospholipid Bilayer Structure of the Plasma Membrane Other components of the plasma membrane • The plasma membrane also contains cholesterol, proteins, and carbohydrates. • Some proteins called receptors transmit signals to the inside of the cell. • Some proteins serve as support structures for the membrane. • Transport proteins move needed substances and wastes through the membrane. The Plasma Membrane Structure of the Plasma Membrane Other components of the plasma membrane • Cholesterol prevents the fatty acid tails of the phospholipid bilayer from sticking together. • Cholesterol contributes to the fluidity of the membrane. The Plasma Membrane Structure of the Plasma Membrane Other components of the plasma membrane • Carbohydrates define the cell’s characteristics • Help cells identify chemical signals The Plasma Membrane Structure of the Plasma Membrane Other components of the plasma membrane • The fluid mosaic model describes the phospholipids in the bilayer as a “sea” in which other components can float and move around. • The different substances in the plasma membrane create a pattern or mosaic on the surface of the cell. The Plasma Membrane Fluid Mosaic Model Video • Fluid Mosaic Model Essential Questions • How does a cell’s plasma membrane function? • What are the roles of proteins, carbohydrates, and cholesterol in the plasma membrane? The Plasma Membrane Section 3 Section 3: Structures and Organelles A Tour of the Cell • https://www.youtube.com/watch?v=1Z9pqST 72is • Crash courses (animal cell then plant) • https://www.youtube.com/watch?v=cj8dDTH GJBY • https://www.youtube.com/watch?v=9UvlqAV CoqY Section 3: Structures and Organelles Eukaryotic cells contain organelles that allow the specializations and the separation of functions within the cell. K What I Know W What I Want to Find Out L What I Learned Cytoplasm and Cytoskeleton • The environment enclosed by the plasma membrane is a semifluid material called cytoplasm. • In prokaryotes, all the chemical processes of the cell take place directly in the cytoplasm. • In eukaryotes, these processes take place within organelles in the cytoplasm. Structures and Organelles The clear, jellylike material between the cell membrane and _________________________________________________ the nucleus that makes up most of the cell _______________________________________ (Most of all cell activity occurs here.) ____________________________________________ CYTOPLASM _______________ Cytoplasm and Cytoskeleton • The cytoskeleton is a supporting network of long, thin protein fibers that form a framework for the cell and proved an anchor for the organelles. • Microtubules are long, hollow protein cylinders that form a rigid skeleton for the cell and assist in moving substances within the cell. • Microfilaments are thin protein threads that help give the cell shape and enable the part or the entire cell move. Structures and Organelles Cell Structures The nucleus • • Contains most of the cell’s DNA, which stores information used to make proteins that determine a cell’s growth, function, and reproduction Is surrounded by a double membrane called a nuclear envelope Structures and Organelles The control center of the cell. ___________________________________________________ Parts of the Nucleus: _________________________________________________ Nuclear Membrane: The membrane that surrounds the nucleus. __________________________________ Nuclear Pores: allow__________________________________ some particles to move in and out of the nucleus Nucleolus __________________________________ Chromosomes __________________________________ NUCLEUS _______________ Cell Structures Ribosomes • Ribosomes are organelles that manufacture proteins. – – Not membrane-bound like other organelles Produced inside the nucleus in the nucleolus Structures and Organelles MAKES PROTEINS FOR THE BODY. _____________________________________ FOUND ON ENDOPLASMICE RETICULUM AND THROUGHOUT __________________________________________________________ CYTOPLASM ___________________________________ RIBOSOMES _______________ Cell Structures Endoplasmic reticulum • The endoplasmic reticulum (ER) is a membrane system of folded sacs and interconnected channels that serves as the site for protein and lipid synthesis. – Two types: rough and smooth Structures and Organelles _________________________________________________________ Moves cell materials from cell membrane to nuclear membrane and _______________________________ visa versa. ____________________________________________ (Can usually find ribosomes on this network.) ENDOPLASMIC _______________ RETICULUM _______________ Cell Structures Golgi apparatus • • The Golgi apparatus is a flattened stack of membranes that modifies, sorts, and packages proteins. Proteins are packaged into sacs called vesicles, which can fuse to the plasma membrane and release the proteins to the environment. Structures and Organelles Packages materials for the cell. _______________________________________ ______________ GOLGI ______________ APPARATUS Cell Structures Vacuoles • • • A vacuole is a membrane bound sac used for temporary storage. Vacuoles store food, enzymes, or waste. Vacuoles are only found in plant cells. Structures and Organelles Stores food and water for cell _______________________________________ ANIMAL CELL PLANT CELL Contains many small vacuoles ____________________________ ________________________________ Contains one large “central vacuole ________________________________ that takes up most of the space _______________________ inside of a plant cell VACUOLE ________ Cell Structures Lysosomes • • Lysosomes are vesicles that contain substances that digest excess or wornout organelles and food particles. They also digest bacteria or viruses that invade the cell. Structures and Organelles Helps digest, (break down), waste materials of cell. _________________________________________________ _______________________________________ LYSOSOMES ____________ Cell Structures Centrioles • • Centrioles are organelles made of microtubules that function during cell division. They are located in the cytoplasm. Structures and Organelles Parts of an ANIMAL cell that help with cell REPRODUCTION ____________________________________________ Cell Structures Mitochondria • • Mitochondria convert fuel particles into usable energy. They have an outer membrane and a highly folded inner membrane that provides surface area for breaking the bonds in sugar molecules. Structures and Organelles Responsible for supplying ENERGY to the cell. __________________________________________________________________________ MITOCHONDRIA ____________ Cell Structures Chloroplasts • • Plant cells and some other eukaryotes contain chloroplasts, which capture light and convert it into chemical energy in process called photosynthesis. They are composed of multiple small disks called thylakoids, which contain the light capturing pigment chlorophyll. Structures and Organelles _________________________________________________ The plant cell parts that contain the green pigment, _________________________________________________ chlorophyll, which are responsible for producing food. __________________________________ ______________________ CHLOROPLASTS Cell Structures Cell wall • Plant cells have a cell wall – a thick, rigid, mesh of fibers that surround the outside of the plasma membrane. – – Protect the cell and give it structure Are made from a carbohydrate called cellulose Structures and Organelles The_________________________________________________________ outer most side of an plant cell, (surrounds the cell membrane), that gives the cell structure, and a specific shape. __________________________________________________________ (More rigid than cell membrane.) __________________________________________________________ ______ CELL ______ WALL Cell Structures Cilia and flagella • • • Cilia are short, numerous projections that look like hair. • Move in tandem like oars in a rowboat Flagella are longer and less numerous than cilia. • Move with a whip-like motion Both are made of microtubules. Structures and Organelles Comparing Cells • Features plant cells have that animal cells generally do not: • Chloroplasts/chlorophyll • Vacuoles • Cell walls Structures and Organelles Centrioles Large central vacuole Organelles at Work • An understanding of different organelles allows for an understanding of cellular processes. • Protein synthesis: • Begins in the nucleus with information contained in DNA • RNA and ribosomes leave the nucleus and produce a protein on the endoplasmic reticulum. • Proteins produced in the ER are sent to Golgi apparatus for packaging. • Packaged proteins are delivered to other organelles where they serve a variety of functions. Structures and Organelles Plant vs animal cell rap • https://www.youtube.com/watch?v=Yu21Shn KhHk Label the organelles of this animal cell. 1. NUCLEOLUS _________________ 2. NUCLEUS _________________ 3. RIBOSOMES _________________ 4. VESICLE _________________ 5. ROUGH _________________ ER 6. GOLGI _________________ BODIES 7. CELL _________________ MEMBRANE 8. SMOOTH _________________ ER 9. MITOCHONDRIA _________________ 10. VACUOLE _________________ 11. CYTOPLASM _________________ 12. LYSOSOME _________________ 13. CENTRIOLES _________________ ANIMAL CELL 2. ____________ 3. ____________ 1. ____________ 8. ____________ 4. ____________ 7. ____________ 6. ____________ 5. ____________ PLANT CELL 2.__________________ 1.__________________ 12.__________________ 3.________________ 4.______________ 11.__________________ 10.__________________ 5.________________ 9.__________________ 8.__________________ 7.__________________ 6.__________________ Essential Questions • What are the structures of a typical eukaryotic cell, and what are their functions? • What are the similarities and differences between plant and animal cells? Structures and Organelles Section 4 Section 4: Cellular Transport Section 4: Cellular Transport Cellular transport moves substances within the cell and moves substances into and out of the cell. Diffusion • Particles in solids, liquids, and gasses are in constant random motion. • Diffusion is the net movement of particles from an area of high concentration to an area of lower concentration. • Additional energy is not required for diffusion because the particles are already in motion. Cellular Transport Diffusion • When diffusion occurs over a long enough time, concentrations will become uniform, and the solution will reach dynamic equilibrium. • Molecules continue to move, but the overall concentration remains the same. Cellular Transport Diffusion Diffusion across the plasma membrane • • • Water can diffuse across the plasma membrane, but most other substances cannot. Facilitated diffusion uses transport proteins to move ions and small molecules across the plasma membrane. Diffusion and facilitated diffusion are types of passive transport – they require no additional energy. Cellular Transport Osmosis: Diffusion of Water How osmosis works • • • Osmosis is the diffusion of water across a selectively permeable membrane. Water is the solvent in a cell and its environment It will move across a membrane until the concentration of solute is the same on both sides. Cellular Transport Video • Diffusion Video • Osmosis Video • DIffusion VS. Osmosis Diffusion and Osmosis • There are three types of solutions. • isotonic – concentrations are equal • hypertonic – solution has more solutes than a cell, cell shrivels and dies • Hypotonic – solution has fewer solutes than a cell, cell expands and may burst hypertonic hypotonic Video • Hypotonic, Hypertonic, & Isotonic Active Transport • • • Sometimes substances must move from an area of lower concentration to an area of higher concentration. Movement of particles across the cell membrane, against the concentration gradient, requires energy and is called active transport. Occurs with the aid of carrier proteins, often called pumps Cellular Transport Active Transport Na+/K+ ATPase pumps • • Are found in the plasma membrane of animal cells Maintain levels of sodium (Na+) and potassium (K+) inside/outside the cell Cellular Transport Active Transport Na+/K+ ATPase pumps • • • Large molecules like sugar need to move against a concentration gradient into the cell. In a process called coupled transport, sugars can pair with Na+ ions and enter the cell through a membrane protein called a coupled channel. Allow sugars to enter through facilitated diffusion – saving energy Cellular Transport Transport of Large Particles • Endocytosis is the process by which a cell surrounds an object in the outside environment in a portion of the plasma membrane. • Exocytosis is the excretion of materials at the plasma membrane. Cellular Transport Exocytosis Video • Endocytosis & Exocytosis • Crash on transport – https://www.youtube.com/watch?v=dPKvHrD1eS 4 Essential Questions • What are the processes of diffusion, facilitated diffusion, and active transport? • What is the effect of a hypotonic, hypertonic, or isotonic solution on a cell? • How do large particles enter and exit cells? Cellular Transport