ADVENTURES WITH A DIGITAL MICROSCOPE A Guide for Project Teachers by MacGregor Kniseley Digital Microscope Case Study Project February – April 2012 Color Photograph in a Newspaper (50x) ProScope HR TABLE OF CONTENTS 1. Introduction ................................................................................................................................ 2. Aligning with Standards ................................................................................................................................... 3. Introducing Early Childhood Students to Magnifiers .............................................................................. 4. Beginning Digital Microscope Activities ...................................................................................................... 5. Additional Digital Microscope Activities ..................................................................................................... 6. Ten GEMS Microscopic Explorations Activities ......................................................................................... 7. Observing Materials in Life, Earth, and Physical Science ....................................................................... 8. Sources of Materials ......................................................................................................................................... 9. Living Organisms by Classification in Science Kits .................................................................................... 10. Living and Nonliving Materials in Science Kits .......................................................................................... 11. Activities for Recording Video and Time-Lapse Images ......................................................................... 12. Microscopy on the Internet ............................................................................................................................ 13. Improving Observation Skills ...................................................................................................... 14. The Private Eye Approach ............................................................................................................................... 15. Teachers Resources (Books, Articles, Video, Internet) ........................................................................... 16. Children’s Literature ......................................................................................................................................... DRAFT: Please Do Not Distribute. ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Introduction Elementary teachers can use different technical resources with their students to engage them in scientific and creative thinking. Students can use digital microscopes to observe and compare the finer details of living and nonliving materials. They can develop an understanding about how scientists use digital microscopes as a scientific instrument to gather data and extend their senses. During investigations, students can use software to capture evidence as still, video, and time-lapse images. Teachers can project magnified images for the entire class to view. A student can interpret a small object projected on a white board by drawing and labeling different features. And, students can think creatively and create artwork while applying an interdisciplinary approach called “The Private Eye.” A handheld digital microscope is easier to use than a traditional compound microscope that requires greater fine motor control. After a 20-30 minute lesson students and teachers can begin using a digital microscope. Unlike a compound microscope, a digital microscope with a computer can record still, video, and time-lapse images, drawing, and measuring. This document provides tips for using handheld digital microscopes in hands on, elementary science and other subjects. The document relates national science education and educational technology standards and explains activities and materials for observing and comparing, and lists teacher resources and children’s literature. 2 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Aligning with Standards Align digital microscope lessons and units to National Science Standards and National Educational Technology for Standards for Students. National Science Education Standards Content Standard A: Science as Inquiry - Abilities Necessary to Do Scientific Inquiry - Understandings About Scientific Inquiry KNOWLEDGE Abilities Necessary to Do Scientific Inquiry Understandings About Scientific Inquiry GRADES K-6 Employ simple equipment and tools to gather data and extend the senses Tools include magnifiers to observe objects and organisms and microscopes to observe the finer details of plants, animals, rocks and other materials Instruments such as magnifiers can provide more information than scientists obtain using only their senses. GRADES 5-8 Use appropriate tools to gather, analyze, and interpret data. Tools include microscopes to view and observe cells and microorganisms. Technology used to gather data enhances accuracy and allows scientists to analyze and quantify results of investigations. Scientists use microscopes to observe objects and phenomena that are otherwise unobservable due to factors size. Source: National Research Council. (1996). National science education standards. Washington DC: National Academy Press. Retrieved from http://www.nap.edu/html/nses/ National Educational Technology for Standards for Students Standard: Ability to demonstrate creativity and innovation Students demonstrate creative thinking, construct knowledge, and develop innovative products and processes using technology. Students: a. apply existing knowledge to generate new ideas, products, or processes. b. create original works as a means of personal or group expression. Standard: Ability to conduct research and use information Students apply digital tools to gather, evaluate, and use information. Students: a. plan strategies to guide inquiry. b. evaluate and select digital tools based on the appropriateness to specific tasks. Source: International Society for Technology in Education. (2007). National educational technology for standards for students NETS-S. Retrieved from http://www.iste.org/standards/nets-for-students/nets-student-standards-2007.aspx 3 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Introducing Early Childhood Students to Magnifiers Most kindergarten and grade 1 students need to learn about focusing objects and concepts of part and whole before using magnifiers. Here are three activities: Blurry vs. Clear (In Focus vs. Out of Focus) Introduce students to "in-focus" and "out-of-focus." The best way to convey the clarity and sharpness associated with focus is to place an image on an overhead projector or digital projector (e.g. a close-up photo of recognizable object) and move it in and out of focus until kids can verbally recognize which is "in focus" and which is "out-of-focus" What does in focus mean (clear, sharp)? What does out of focus mean? (blurry, hard to see) Part and Whole Teach the concept of part versus whole using an empty paper towel tube or toilet paper tube. Have students hold the tube up to the bones around the eye and look through an empty paper towel tube or toilet paper tube at their arm or another student across the room. Describe what part of the whole you are seeing. Mystery Picture Challenges Use the digital microscope and record a set of images for “Mystery Picture Challenge.” Record images of parts of wholes. Have students guess the “whole.” For examples, see the following url: http://mason.k12.il.us/havanajh/mystery/ Then have students use the digital microscope and software to create their only mystery picture challenge. Beginning Digital Microscope Activities Here are several activities for introducing digital microscopes to students for the first time. With only one digital microscope for the classroom, use the microscope with the whole class and in a learning center. To increase active participation, combine the use of the digital microscope and a classroom set of low magnification plastic magnifiers. Single Lens (3x) Tri-Lens (2X, 6X, 8X) Set of 30 = $28.50 The Private Eye Loupe (5x) * Set of 30 = $88.50 . * Two loupes nested gives 10x magnification. Proper Use of the Digital Microscope Use direct instruction to teach students how to set up and operate the digital microscope and flexible stand. 4 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute To learn how operate Bodelin Technology’s ProScope HR, go to http://www.bodelin.com/proscopehr/how_it_works/ Click Demonstration Videos (on the right) and PDF Microscope Documentation (on the left). Create rules together for the safe and responsible use of the equipment. Some ground rules include: 1. Respect the equipment. Replacement costs are $450. 2. Store and carry the equipment in the plastic case. 3. Supervise the students while they’re using the equipment. Teach students to hold the digital camera carefully. 4. Keep the lens dry. If the plastic lens comes in contact with any wet or sticky substance, clean and dry it immediately. 5. Work with the digital microscope on dry tables. Clean up surrounding spills immediately . Keep beverages away from laptop and digital microscope. Discover Something New in an Everyday Objects The Penny Begin by directing students to draw an enlarged view of the head/tails sides of a penny on an 8 1/2 x 11 inch sheet of paper. Include as much detail as possible. Then, use the digital microscope to project images of the penny. Before observing the penny, clean the penny with some vinegar so it’s bright and shiny. You can observe Lincoln inside the Lincoln Memorial, initials FG representing the designer Frank Gasparro. Five Dollar Bill Observe and count the names of states on the Lincoln Memorial. Notice the different ink colors and patterns of ink, such as the random yellow numbers. Fingerprints Use a water-soluble inkpad to stamp your fingerprints and observe the details using the digital microscope. Create a detective game by having the detective leave the room while one criminal makes a fingerprint. The detective can examine the fingerprint and compare everyone’s fingerprints to identify the criminal who made the fingerprint. Sugar and Salt Place some sugar and salt on black cardboard. Keep them separate and observe the crystals. How are they similar and different? Create a Venn Diagram or Same-Different Chart. Flat Materials First Observe and draw flat objects such as five-dollar bill, color newspaper photo, or fingerprint. Then, observe larger, solid objects and living things that move. Observe Objects Around School Students don’t need to go far to find interesting materials to observe and compare: On You: skin, fingernails, hair, pocket lint, fabric, watch, shoe soles 5 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute In the Classroom: chalk, pencil points, pencil shavings, eraser crumbs, paper clips, paper, dust In the Cafeteria: small pieces of bread, fruit, chips, and salad items On the Playground: pavement, sides of buildings, rocks, leaves, soil, insects, dandelions, grass On the Principal’s Desk: paper clips, pens, staples, and “Catch You Being Good” reward slips 6 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Additional Digital Microscope Activities Microscopic Explorations Learning Center Set up a GEMS Microscopic Explorations learning center in the classroom. Rotate small groups of students through the center. Create small group cooperative roles (e.g., Reader, Starter, Checker). Students can rotate roles with each new activity. See p. 8. Source: Brady, Susan & Willard, Carolyn. Microscopic explorations. (2003). Berkeley, CA: Lawrence Hall of Science, University of California at Berkeley. http://lhsgems.org/GEMmicro.html The Private Eye Approach “The Private Eye” is an instructional strategy that includes close observation of everyday objects using a magnification tool, drawing, and thinking by analogy. The magnifier enhances the observer’s focus on details and patterns. While observing, the student things by analogy “What does this remind me of?” The observer “sees with meaning,” connects form with function, solves problems, and draws inferences. See p. 14. Source: Ruef, K. (1992). The Private Eye, (5x) Looking/Thinking by Analogy, A Guide to Developing the Interdisciplinary Mind, Hands-On Thinking Skills, Creativity, Scientific Literacy. Seattle: The Private Eye Project. Secret Message Use “Object Palette” in Microsoft Office and make a 3 inch black dot. Insert a text box and type a secret message inside the black dot. Type the secret message in white font on the dot then reduce the font size to 4 pt - so the font is too small to read with the naked eye. Print it out using a laser printer. Read the secret message with a 50x lens. What did Mars say to Saturn? Observe black dot with 50x lens and find the secret message. Secret Message Still image (50x) from digital microscope Microscopic World Bookmark Insert images recorded from the digital microscope in a table in a Microsoft Word document. Microscopic World Bookmark 50 x images of a newspaper photo 7 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Shapes and Colors Observe different materials and look for shapes and colors in natural materials. Example of shapes and materials to observe: Square – salt crystals, iron pyrite, fabrics Rectangle – fabrics Triangle – tips of fern fronds, flower (trillium), fish scales, spider webs, Circle – drops of water, spores on fern frond, radish seeds, mushroom cap, acorn shell, whorl patterns on fingerprints Heart – red bud, aspen Patterns in Nature Observe different materials and look for form and symmetry in nature. To prepare, view a six-minute video by biologist and photographer Amy Lamb. http://www.amylamb.com/films.htm The 6-minute video is based on her 2007 US Botanical Garden exhibition. She used her photographs to illustrate patterns in a nature. She explains how beauty can be found through form (branches, layers, spirals) and symmetry (radial and bilateral ) in flowers and other materials in the natural world. Examples of patterns and materials to observe using the digital microscope: Form: Branches - feathers, branches on twig Layers - buds, fish scales, snakeskin Spirals - snail shell, fiddlehead fern Symmetry: Radial - snowflake, starfish, sunflower Bilateral - insects Draw Fingerprints and Assemble a Fingerprint Quilt Have students make colorful enlarged drawings from observing their fingerprints. For examples of drawings by students, see “Fingerprint Gallery” at The Private Eye: http://www.the-private-eye.com/html/galleries/fp2html/gal2.html Assemble fingerprint drawings into a “fingerprint quilt.” See “Fingerprint Poster” at The Private Eye: http://www.the-private-eye.com/html/aboutTPE/WhatsNew.html#fingerprintposter Sand at the Beach Collect sand from different parts of the beach. Does it look the same? Collect sand from different beaches. Do they look the same? Growing Mold Grow mold on bread and observe different molds. Place a moist paper towel in bottom of a plastic container. Place a slice of white bread on top of the wet paper towel. Cover the container with plastic wrap. Store in a dark place for 3-7 days. There are more than 85,000 8 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute known kinds of mold. Some are beneficial such as manufacturing beverages and cheese (Roquefort, blue, and Gorgonzola) and production of penicillin. Some are harmful and cause disease in plants (mildew on grapes, rot on peaches) or in people (ringworm and athlete’s foot). After a week, you can see a variety of molds growing on the bread. Each color is a different colony. Black mold called rhizopus is a common variety that can be examined with 10x magnifier. The small round balls on the end of the slender stalks are cases containing spores. When the cases ripen, they release the spores into the air, and some fall on the bread to form new mold. Drawing Scientifically Teach students to observe and draw the finer details of an object – to draw like a scientist. Careful drawing while closely observing can improve observation skills. Drawing slows a student down and helps them focus on the details of material. Younger students might draw cartoonish figures that feature the obvious properties color and shape. Demonstrate the difference between a cartoon and scientific drawing of an object. For example: Make a drawing of a cartoon drawing of insect or spider with a circle and a dot in the middle represents the eye. Then draw the eye more scientifically. Have them draw a details of the insect or spider eye scientifically. Improving Visual Memory Students can observe an object using the digital microscope for a period of time and identify five features to remember to draw. Then, students return to their workstation to draw the object and label five features they can recall. They can return to the digital microscope and check to see if the drawing is accurate. NOTE: This activity relates to a fun visual memory game, called Kim’s Game. In Rudyard Kipling’s novel Kim, the teenage hero Kim plays a game with a shop owner while training in secret to be a spy. In the classroom, a leader can present a tray with 20 different objects. Students observe the objects for several minutes and try to remember all of the objects. Next, the leader removes one of the objects when students are facing another direction. Then, the students observe the tray and identify the missing object. 9 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Ten GEMS Microscopic Explorations Activities Microscopic Explorations is a Grades 4-8 unit featuring ten learning-station activities designed to encourage students’ curiosity using different materials representing life, earth, and physical science. The book includes “behind the scenes” scientific information about the materials used for observation. 1. Up Close Construct water drop magnifiers (1 inch by 2 inch cover stock with hole punched, transparent tape) to observe small print in a newspaper. Drop water on top of non-sticky side of tape and observe small print of newspaper. 2. Fingerprint Ridges Make impressions of fingerprints using pencils and a piece of transparent tape. Fin details of ridge patterns used by forensic scientists to identify fingerprints. 3. Dots and Dollars Observe and compare the dots on color newspaper photos, magazine pictures, and other images and other images . Understand how size and distance apart of dots on color photos can be combined to give the appearance of a range of other colors. 4. Fabrics Observe and compare a variety of fabrics (.g., light-colored woven, knitted, and pressed). Understand how fabrics are made by observing their appearance under the digital microscope. 5. Salts Observe and compare crystals of various salts (e.g., rock salt, table salt, alum, boric acid, Epsom salt) cardboard “slides” (1 inch by 2 inch cover stock with hole punched, transparent tape). Observe crystals form by adding Epsom salts to a crystal “suncatcher” (crayon-colored clear plastic lid) 6. Sand Observe and compare sand samples from several geographic locations based on the color, size and shape of the sand grains. Sand samples can be found from the International Sand Collector’s Society. They locate and mark the source of their sand on a map. 7. Kitchen Powders Observe and compare color, size and shape of seven white powders found in the kitchen (e.g., table salt, cream of tartar, laundry detergent, baking soda, cornstarch, white granulated sugar, flour). 8. Small Creatures Observe structures of entire of parts (wings, eyes, legs, antennae) of dried insects, spiders, and isopods. 10 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute 9. Brine Shrimp Observe and compare the overall appearance, structures (e.g., legs, head, eyes, gills, eggs sacs), and movement patterns of adults, larvae and eggs of live brine shrimp (also called “sea monkeys”). 10. Pond Life Compare plant, animal, and one-celled organisms found in a pond. Source: Brady, Susan & Willard, Carolyn. Microscopic explorations. (2003). Berkeley, CA: Lawrence Hall of Science, University of California at Berkeley. http://lhsgems.org/GEMmicro.html 11 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Observing Materials in Life, Earth, and Physical Science Observe and compare different materials that relate to your life, earth, and physical science curriculum. Here are interesting materials to observe. LIFE SCIENCE 1. Butterfly and Dragonfly: wings 2. Dandelions: flower head, leaves 3. Earthworm: Blood flowing in an earthworm. Using a 10x lens, place an earthworm in a Petri dish with some water. Record the blood flowing. 4. Earthworms (blood flowing) 5. Feathers: shaft and barbules; different types of feathers 6. Ferns: house ferns - fronds and spores 7. Fingerprints: Prints from different fingers; big toe and thumb; different people’s fingerprints 8. Fish scales 9. Flowers: different reproductive parts (stamen, pistil) 10. Flowers: rose, forget-me-nots; tulips; foxglove 11. Fruit: strawberries, oranges; star fruit, kiwi 12. Fur or hair: dog and cat 13. Granola 14. Human body parts: skin, hand, fingers, arm, leg 15. Insects: stages of development [e.g., larvae of mealworm (darkling beetle) and wax worm (wax moth); structures (eye, antennae, legs, body parts – head thorax, abdomen) 16. Isopods: pillbugs and sowbugs 17. Lichens, liverworts, moss, mushrooms 18. Movement of Organism: Using a 10x lens, record movement patterns of an invertebrate in a clear plastic container organism. 19. Owl pellet: bones of prey 20. Plant buds: buds that haven’t opening, that are beginning to open, and buds that have opened 21. Popped popcorn 22. Rice: basmati, enriched white, long-grained brown 23. Seeds of every day foods: strawberry, peanut, sunflower 24. Shells: quahog, oyster, slipper, scallop, barnacle, periwinkle, razor 25. Spices: peppercorns, anise, dill seed, celery seed 26. Sprouts: fresh, whole, edible sprouts – mung bean, alfalfa. 27. Tree and shrub twigs 28. Tree bark: birch, sycamore, pine, oak 29. Tree clippings: cedar branch, holly 30. Tree leaves: conifer and deciduous leaves (dusty miller, sage, foxglove, ferns 31. Tree seeds: maple, sycamore, acorn, walnut 12 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute EARTH SCIENCE 1. Minerals: quartz, mica, iron pyrite 2. Rocks : granite, limestone, marble 3. Sands from different parts of the beach and geographic locations of the world 4. Soil: sand, clay, humus 5. Water: property of density at different temperatures - cold water in room temperature water. Position a low power lens to capture the side view of a clear glass. Add a small plastic vial of red-colored cold water (iced) and empty at the top of the glass filled with clear room temperature water. Record the downward movement of the denser, red water. PHYSICAL SCIENCE 1. Clothing: coat, sweater, shirt, soles of shoes 2. Crystal formation: crystals forming from drops of mixture of 1 part Epsom Salt and 1 part water 3. Crystals: dissolving of salt or sugar (video/time lapse) 4. Fabrics: microfiber cloth, burlap fabric, yarn 5. Kitchen powders: table salt, cream of tartar, detergent, baking soda, cornstarch, white granulated sugar, flour 6. Paper and ink: printed text/photos: book, newspaper, magazine, wrapping paper 7. Paper chromatography: Make a stripe with a bold black water-soluble ink marker on white paper towel. Add drops of water on the black ink and record the image of water separating black ink into different colors. 8. Phase change - liquid to gas: Place a small drop of liquid water on a surface and record the evaporation. 9. Phase change - solid to liquid: Place a small piece of ice on a surface and record the melting. 10. Postage stamps 11. Salts: rock salt, table salt, alum, boric acid, Epsom salt 12. String: thread, cotton and nylon string 13 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Sources of Materials 1. Home; Hardware, Fabric, Grocery, Pet Stores 2. Science Supply Companies: Connecticut Valley Biological http://www.connecticutvalleybiological.com/ Carolina Biological http://www.carolina.com/ Delta Education http://www.deltaeducation.com/ Pellets, Inc. http://www.pelletsinc.com/ 3. The Private Eye Jewelers’ Loupes and Specimen Kits http://www.the-private-eye.com/ 14 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Living Organisms by Classification in Science Kits Here are organisms classified by kingdoms included in FOSS and STC science kits supplied by East Bay Educational Collaborative Science Materials Resource Center: ANIMALS 1. Annelids - earthworms 2. Mollusks – ramshorn snails, pond snails, earthworms 3. Arthropods Insects – American painted lady butterfly, wax moth (waxworm), darkling beetle (mealworm), cricket, and Bess beetle Millipede Crustacean - crayfish, daphnia, isopods , pill bugs, sowbugs 4. Fish - guppies and goldfish PLANTS 1. Brassica (mustard), alfalfa, beans, corn, pumpkin, sunflower, variety of fruits and vegetables 2. Pine seedlings 3. Moss 4. Elodea, cabomba, duckweed FUNGUS Lichen PROTISTS 1. Culture of blepharisma 2. Culture of volvox and other algae STC: Science and Technology for Children. Developed by National Science Resources Center Published by Carolina Biological FOSS: Full Option Science System Developed by Lawrence Hall of Science Published by Delta Education Source: http://ebecri.org/content/kitcon tents 7 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Living and Nonliving Materials in Science Kits K GRADE SCIENCE MODULE Animals 2x2 PROGRAM FOSS Grade 1 Organisms STC Grade 2 Insects FOSS Grade 2 Pebbles, Sand & Silt FOSS Grade 3 STC Grade 3 Plant Growth & Development Structures of Life FOSS Grade 3 Water FOSS Grade 3 Changes STC Grade 3 Rocks & Minerals STC Grade 4 Electric Circuits STC Grade 4 Earth Materials FOSS Grade 4 Ideas & Inventions FOSS Grade 4 Ecosystems STC Grade 4 Land & Water STC Grade 5 Microworlds STC Grade 5 Grade 5 Environments Landforms FOSS FOSS STC: Science and Technology for Children. Developed by National Science Resources Center Published by Carolina Biological EXAMPLE OF MATERIALS TO OBSERVE Goldfish, guppies elodea, mystery snails, ramshorn snails, red worms, earthworms, pillbugs, sowbugs; fish food, shells, potting soil, aquarium dip net Lichen/moss mats, pine seedlings, elodea, cabomba, guppies, pond snails, pillbugs, sowbugs, Bess beetles (Millipedes) American painted lady butterfly, wax moth (waxworm), darkling beetle (mealworm), milkweed bugs; screening, pabulum, millet, shelled sunflower, soil Basalt, scoria, tuff, clay, cornstarch, humus, fiver rocks, gravel pebbles, sand, sandpaper, screens brassica (mustard) – different stages of development; lima beans, soil, dried honey bees crayfish; kidney bean, lima bean, pea, popcorn, corn, sunflower, variety of fruits and vegetables; dry cat food, gravel, nutrient powder, yarn profile of water drops on a penny; movement of cold and hot water through room temperature water Cover, field corn, pea, radish, soil, Brine shrimp eggs, fish food, pabulum, antacid tablet, fabric, gravel, pepper, kosher salt, steel wool pad, sugar cube, granular sugar calcite, biotite, feldspar, fluorite, galena, gypsum, halite, hematite, magnetite, muscovite, quartz, sulfur, basalt, conglomerate, gneiss, granite, limestone, marble ,obsidian, pumice, sandstone, schist, shale, slate, D-cell battery, paper fasteners, brass washer, wire insulation, flashlight bulb, diode, fiberglass screen, wire screen, brass screw, golf pencil, pipe cleaner, nail, marble gravel, calcite, feldspar, fluorite, gypsum, hornblende, mica, quartz, basalt, cumberlandite, limestone, marble, sandstone, sand, shell, coral pieces, Fingers (fingerprinting), rubber bands, water-based markers, fabric, netting, alfalfa, mustard, rye grass, Algae mix, elodea, duckweed, pond snails, guppies, crickets, isopods; salt; fiberglass screen, soil clay, humus, sand, markers, rubber bands, Velcro, mustard seed, rye grass, one celled organisms: Blepharisma, Vinegar eels, Feathers, fish scales, gelatin, grits, hay, marble, microfiche, Epsom salt, table salt, sand, wire screen , poppy seed, year darkling beetles, isopods, goldfish, elodea, gammarus/daphnia, pond snails, duckweed sand, clay, cotton swab applicator FOSS: Full Option Science System Developed by Lawrence Hall of Science Published by Delta Education 8 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Activities for Recording Video and Time-Lapse Images Second Hand Movement on a Wristwatch Record the second hand movement on a analog wristwatch. Dissolving Crystals Using a 50x lens, sprinkle a few crystals of salt or sugar on a surface. Add a drop of water and use time-lapse video settings to record the particles flying off the crystals into the liquid solution. Formation of Crystals Place a drop of a hot mixture of Epsom salt and water (1 part Epsom salt and 1 part water) on a hole-punch slide. Record the formation of crystals. Phase Change: Solid to Liquid Place a small piece of ice on a surface. Record the melting. Phase Change: Liquid to Gas Place a small drop of liquid water on a surface. Record the evaporation. Adding Cold Water to Room Temperature Water Add a small plastic vial of red-colored cold water (iced) and empty at the top of the glass filled with clear room temperature water. Use a 10x lens and record the downward movement of the denser, red water. Movement of Organism Use a 10x lens and record movement patterns of an invertebrate in a clear plastic container organism. Blood Flowing in an Earthworm Place an earthworm in a Petri dish with some water. Use a 10x lens and record the blood flowing. Capillary Action Make a bold black water-soluble ink stripe on white paper towel. Add drops of water on the black ink. Use a 10x lens record the image of the capillary action of water separating black ink into component colors. 9 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Microscopy on the Internet Rogers, Kirsteen. (2006). The Usborne complete book of the microscope. London, UK: Usborne Publishing. The Internet-linked printed book includes recommended URLs to extend the learning from photographs and information about the microscopic world of the human body, plants, fungi, invertebrates, rocks, minerals, crystals, and nanotechnology. http://www.usbornequicklinks.com/usa/usa_entity_pages/usa_select_page.asp?lvl=1&menu=s73&id=797 Magnification Module. Retrieved from http://micro.magnet.fsu.edu/primer/virtual/magnifying/index.html Students can explore the effect of increasing magnification (equivalent to changing microscope objectives) on the ability to resolve features in a sample in his interactive Java tutorial exploring. Virtual Scanning Electron Microscopy. Retrieved from http://micro.magnet.fsu.edu/primer/java/electronmicroscopy/magnify1/index.html Students can explore how specimens appear when magnified in the virtual SEM in his interactive Java tutorial exploring. Microscope Imaging Station. Retrieved from http://www.exploratorium.edu/imaging_station/index.php Students can explore high-resolution images and video using research-grade microscopes. A range of images and movies illustrate the diversity of what can be seen with a microscope. The Exploratorium’s website includes information about the magnified images and activities. Microscopy Ideas. Retrieved from http://members.ozemail.com.au/~macinnis/scifun/micrscop.htm Teachers and students can learn about materials for microscopy provided by Australian science writer, Peter Macinnis. 10 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Improving Observation Skills Students are often challenged making observations exact and detailed. They jump to conclusions or confuse observations with inferences. These are the expected observation abilities for early and later stage development. Early Stages uses more than one of the sense to make observations identifies the obvious features of an object or event Later Stages makes use of several senses notices relevant details of the object and its surroundings identifies similarities and differences discerns the order in which events take place uses aids to the senses for study of details makes measurements or comparisons using appropriate instruments To improve students’ ability to observe, try these strategies: 1. Facilitate observing of living and nonliving material by asking attention-focusing, measuring, and comparing questions. See Observing Questions below. 2. Have children draw as part of the process of observing. It slows them down and causes them to spend more time observing and interpreting the material. 3. Make an observation guide before students observe. See below. 4. Distinguish between observations that are accurate and inaccurate. 5. Distinguish between an observation and an inference. Questions to Improve Observation Type of Question Attention-focusing Form of Questions Have you seen...? Do you notice...? More complicated observation questions follow: What is it? What does it do? What does it show about itself? What happens? What do you find inside or outside? What do you feel, see, hear, and smell? 11 Examples * Have you seen the carapace of the crayfish? Show me where it is. Do you notice the swimmerets? Where on the plant is the bean pod developing? ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Measuring and counting Comparison How many? How much? How long? How strong? How heavy? How often? In how many ways are ---alike and how do they differ? Classifying, attribute games, making identification keys, Venn diagrams, or tables of collected data are based on comparison questions How many legs does your crayfish have? How long is your crayfish? How long does it take for a bush bean plant to complete its life cycle? How many bean pods do you see? How are the seedlings alike and different? Which of the fruits has the fewest seeds? In what ways are the male and female crayfish alike? How do they differ? Which is the longest crayfish? the shortest? * Examples of questions – FOSS Structures of Life science kit Using an Observation Guide Before observing an object or material, create a guide with your students and develop relevant descriptive language. Ask students to anticipate what they might observe with a digital microscope for features of color, size, number, shape, and shape. Ask students to suggest language to use for each feature. Provide a chart like this to describe properties they observe. COLOR SIZE SHAPE Create a guide to encourage students to use other senses . Other Features to Observe: Parts Quantity Markings Wet or dry Shadows Luster (shiny or dull) Patterns - Geometrical Shapes (including vertex, edge, 3-D perspective) Lighting/Reflections 12 SHAPE ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Measurement (including mass, volume, length, height, depth, width) What is happening? Change Behavior pattern Movement, Location Expressions or gestures Speed Sequence in time Source Checkovich & Sterling, (2001, January). Oh say can you see. Science and Children. pp. 32-3 Graphic Organizers for Observing and Comparing Students can observe, compare, and organize their thinking using Venn Diagrams and Same – Different T charts. 13 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute THE PRIVATE EYE APPROACH I. Collecting, Wondering, and Close Observation The most beautiful thing we can experience is the mysterious. It is the source of all true art and science. --Albert Einstein Tools: loupe, measuring tape, clipboard, zipper bags, paper, pens, magnifying boxes Find an object and observe the finer details. What do you notice? Where is it? How does it feel? Smell? Hear? Taste (?) Measure it. Count it. Weigh it. II. Looking for Patterns— Thinking by Analogy and Writing All perception of truth is the perception of analogy; we reasons from our hands to our head. --Henry David Thoreau III. Loupe-Drawing (Close Observation and Aesthetics) Paleontology is a very visual inquiry. All paleontologists scribble on napkins at coffee breaks, making sketches to explain their thinking. --Robert Bakker American Palentologist Artist A. What else does it remind me of? B. What else doe it look like? It reminds me of… To draw is to look closely. It looks like… Visually explore the object with your loupe, putting pen to paper. Look, draw, look, draw, look . . . 14 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute V. Theorizing-Hypothesizing IV. Change the Scale (More Loupe Drawing, Close Observation, and Aesthetics) The ability to imagine a change of scale—to imagine something bigger or small, more or less intense, more or less crowded is essential to understanding in many areas: demographics, overpopulation, issues of ecology, man’s impact on the environment, the impact o toxins building and passing thresholds, and more. As a habit of seeing—the ability to change scale is part of the ability to change paradigms. Close (1X) Why did it remind me of that? Why do I think it is like that? What do I think is the design’s function? If it reminds me of that, I wonder if it might function like that in some way? From the list of analogies, pick any five and explain why it reminded you of that. Consider shape, texture, color, smell, size, movement, feelings, etc. Use your analogies organism to survive? Draw as you see the object with your eye only. Choose three analogies from above as clues to help you answer ”Why is it like that?” Closer (5X) Search the first view for a small portion to enlarge. Draw a section as you see it with one loupe Closest (10X) Search the second view for a small portion to enlarge. Draw a section as you see it with two loupes. VI. Planning Investigations NOW, how would you test your theories? 15 It could be this because______ It could be this because______ It could be this because______ ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Object: Date: Observing Closely By Loupe Drawing First, use your senses and explore the object with your loupe, putting pencil to paper. Look, draw, touch, draw, smell, draw, measure/count/weigh, draw, hear, draw…. Analogizing Hypothesizing 1 What does it remind me of? What else does it look like? It reminds me of … It looks like…. Why did it remind me of that? From your analogy list, pick 3 and explain why it reminded you of that. Consider shape, texture, color, smell, size, movement, and feelings. 1. 2. 3. 4. 5. 6. 7. Hypothesizing 2 Use your analogies as clues. Ask yourself: If it reminds me of _________, I wonder if it might act like that to help this organism survive? Choose 1 analogy from above as a clue to help you answer “Why is it like that?” It could be because . . . Planning an investigation NOW, how would you test your theories? 16 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Teacher Resources Here are suggestions of books, articles, video, and Internet resources for teaching about magnification and microscopy. Books Ahouse, Jeremy & Barber, Jacqueline. (2000) Fingerprinting. Berkeley, CA: Lawrence Hall of Science, University of California at Berkeley. Fingerprinting is s 46-page teacher guide for a Grades 4-8 unit where students compare fingerprints and observe the variations of fingerprints. They learn about fingerprinting as an aspect of forensic science. They make fingerprints using pencil and transparent tape. They observe, compare, and classify the fingerprints. They apply ability to classify to solve a crime using a mystery, Who Robbed the Safe?, developed by the authors or create their own characters and plots for a mystery. http://lhsgems.org/GEM180.html Beals, Kevin & Willard, Carolyn. (1999). Mystery festival. Berkeley, CA: Lawrence Hall of Science, University of California at Berkeley. Mystery Festival is 268-page teacher guide for a Grades 2-8 unit using a classroom learningstations. Students investigate a "crime scene," conduct tests on the evidence, analyze the results, and solve the mystery. http://lhsgems.org/GEM350.html Brady, Susan & Willard, Carolyn. Microscopic explorations. (2003). Berkeley, CA: Lawrence Hall of Science, University of California at Berkeley. Microscopic Explorations is a Grades 4-8 unit featuring ten learning-station activities designed to encourage students’ curiosity using different materials from life, earth, and physical science. http://lhsgems.org/GEMmicro.html Headstrom, Richard. (2012, March). Adventures with a hand lens. Dover Publications. A 240-page guide to observing the living world using a magnifier: flowers and grasses, fish scales, moth and insect wings, egg cases, buds, feathers, seeds, leaf scars, moss, molds, ferns, common crystals. Includes 209 exact line drawings. http://www.amazon.com/Adventures-Hand-Lens-Richard-Headstrom/dp/0486233308 Headstrom, Richard. (1977). Adventures with a microscope. Dover Publications. A 240-page guide to observing the natural world using a simple microscope. Students can observe structures of numerous microscopic animals, everyday objects and foods really look like at the cellular level. Students can learn how to prepare specimens and slides. http://www.amazon.com/Richard-Headstrom/e/B001HMPG90/ref=ntt_athr_dp_pel_1 National Science Resources Center. (1991). Microworlds. Science and Technology for Children. Washington, DC: Carolina Biological. Microworlds is a 123-page teacher guide for Grades 5-6 science kit. During the eight-week, 16lesson unit, students learn how to observe, how to record their observations, using both writing 17 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute and drawing. They learn about the properties of magnifiers and observe a variety of living and nonliving materials. They learn to use 3x-6x hand lenses and 30x microscopes. Using slides, cover slips, droppers and other related apparatus, they observe organisms found in ponds and a hay infusion. http://www.nsrconline.org/curriculum_resources/Mic_overview.html Ruef, Kerry. (2003). The private eye - (5X) looking / thinking by analogy: A guide to developing the interdisciplinary mind. Lyle, WA: The Private Eye Project. A 240-page guide to developing scientific and creating thinking by using The Private Eye approach. You can view examples of student writing, drawing and theorizing, more than 200 lesson plans, and activities for enhancing different subject areas. The Private Eye process builds communication, problem solving, and observing skills. http://www.the-private-eye.com/html/materials/MATmenu.html#book Articles Ashbrook, Peggy. (2008, February). Observing with magnifiers. Science and Children. pp. 18-20. Checkovich & Sterling, (2001, January). Oh say can you see. Science and Children. pp. 32-35. Coskie, Tracy L. & Davis, Kimberly J. (2009. Summer). Larger than life: Introducing magnifiers. Science and Children. pp. 64-65. Farland, Donna. (2008, February). Close, closer, closest. Science and Children. pp. 40-42. Goode. Laurie & Mullins, Vanessa. Digital microscopes in elementary classrooms. Retrieved from http://www.oercommons.org/courses/digital-microscopes-in-elementaryclassrooms/view McNall, Rebecca L & Bell, Randy L. (2004, January). Discovering flowers in a new light. Science and Children. pp. 35-39. Tessmer, Michael & Cowlishaw, Richard. (2011, December). Time for slime. Science and Children. pp. 38-41. Travaille, Madelaine & Adams, Sandra. (2006, April/May). Using digital microscopy. The Science Teacher. pp. 50-54. Video Eames, Charles & Eames, Ray. (1977). Powers of ten. Retrieved from http://www.powersof10.com/ A 9-minute film. “Powers of Ten takes us on an adventure in magnitudes. Starting at a picnic by the lakeside in Chicago, this famous film transports us to the outer edges of the universe. Every ten seconds we view the starting point from ten times farther out until our own galaxy is visible only a s a speck of light among many others. Returning to Earth with breathtaking speed, we move inward- into the hand of the sleeping picnicker- with ten times more magnification every ten seconds. Our journey ends inside a proton of a carbon atom within a DNA molecule in a white blood cell. “ Video: http://www.powersof10.com/film Interactive web site: http://www.powersof10.com/ 18 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Haeckel, Ernest. Art forms in nature. Retrieved from http://www.youtube.com/watch?v=shqoFrJCU-o This 7-½ minute video includes images from pages of Ernst Haeckel’s drawings from the book Art Forms in Nature. Ernst Haeckel (1984-1919) German biologist, naturalist, philosopher, physician, professor and artist discovered, described and named thousands of new species. Lamb, Amy. Patterns in nature. Amy Lamb Studies. Retrieved from http://www.amylamb.com/films.htm The 6-minute video is based on her 2007 US Botanical Garden exhibition. She used her photographs to illustrate patterns in a nature. She explains how beauty can be found through form (branches, layers, spirals) and symmetry (radial and bilateral ) in flowers and other materials in the natural world. Website: http://www.amylamb.com Nuridsany, Claude & Pérennou, Marie. (1996). Microscosmos. Galatée Films. A documentary of insect life in meadows and ponds, using close-ups, slow motion, and timelapse photography. Examples of images include bees collecting nectar, ladybugs eating mites, snails mating, spiders wrapping their catch, a scarab beetle relentlessly pushing its ball of dung uphill, lines of caterpillars, an underwater spider creating an air bubble to live in, and a mosquito hatching. Full video: http://www.youtube.com/watch?v=m3JsZMr3NFw&feature=related Part 1 http://www.youtube.com/watch?v=-BseGLUTkD8 (There are nine segments of the full video on YouTube) Internet Resources The Private Eye Project. Retrieved from http://www.the-privateeye.com/html/aboutTPE/Wht1_whatis.html TPE is a project devoted to materials and training for an interdisciplinary approach to thinking that begins with close observation. Students develop scientific and creating thinking using everyday objects, a unique 5x jeweler's loupe (designed by TPE), close observation, drawing, and simple questioning. View the gallery of images of results of loupe drawing. Project MICRO - Microscopy Society of America. Retrieved from http://www.microscopy.org/education/projectmicro/ Books, media, and websites for teaching K-12 students about microscopy. Exploring the World of Optics and Microscopy. Retrieved from http://www.micro.magnet.fsu.edu Color photographs taken by an optical microscope – birthstones, ice cream to integrated circuits and ceramic superconductors. 19 ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute Photo Microscopy. Retrieved from http://www.nikonsmallworld.com/ Images of materials using digital photography through a microscope. http://www.microscopyu.com/smallworld/gallery/index.html Microworlds Festival. Retrieved from http://www.mnmicroscopy.org/ProjectMicro/Stations/Welcome.html Magnified images of materials from GEMS Microscopic Explorations. Center for Disease Control and Prevention. Retrieved from http://phil.cdc.gov/phil/home.asp Photographs, illustrations, multimedia files associated with diseases and pathogens. Microworlds. Retrieved from http://notes-from-dreamworlds.blogspot.com/ A blog maintained by Daniel Stoupin, a biology student and microscopic photographer Dennis Kunkel Microscopy Education. Retrieved from http://education.denniskunkel.com/ A web site with images and links to other microscopy web sites. Dennis Kunkel contributed photographs to Vicki Cobb and Alexandra Siy books. CSI – The Experience: Web Adventure. Retrieved from http://forensics.rice.edu An interactive exhibit, activities, focusing on the television show CSI. 20 Children’s Literature Here are titles of children’s literature for teaching about magnification, microscopes, photographing small objects, and close observation. Cobb, Vicki, Harris, Andrew & Kunkel, Dennis. (2009). Your body battles a broken bone. Millbrook Press. CRRAACK! Breaking a bone hurts! But did you know that it's the start of a battle for your body to heal itself? That ache happens because damaged nerve cells send out a cry for help. In response, red blood cells rush in to bring oxygen and nutrients. White blood cells clean up the dead bone and muscle cells. Blood also brings fibroblast cells. They build a sort of scaffolding around the break so that new bone will fill in. And stem cells arrive to transform themselves into cells that will make brand-new bone. The whole area becomes an active construction site as your body battles the damage and heals itself. Get a close look at this body battle with comic illustrations and groundbreaking photomicrographs. The photomicrographs magnify the actual cellular processes tens of thousands of times, offering you a front-row seat for all the action. Other similar Vicki Cobb Books: Your Body Battles a Cold, Your Body Battles a Skinned Knee, and Your Body Battles an Earache. Author’s Web Site: http://www.vickicobb.com/ Martin, Jacqueline Briggs & Azarian, Mary. (1998). Snowflake Bentley. Houghton Mifflin. From the time he was a small boy, Wilson Bentley saw snowflakes as small miracles. And he determined that one day his camera would capture for others the wonder of the tiny crystal. Bentley's enthusiasm for photographing snowflakes was often misunderstood in his time, but his patience and determination revealed two important truths: no two snowflakes are alike; and each one is startlingly beautiful. His story is gracefully told and brought to life in lovely woodcuts, giving children insight into a soul who had not only a scientist's vision and perseverance but also a clear passion for the wonders of nature. "Of all the forms of water the tiny six-pointed crystals of ice called snow are incomparably the most beautiful and varied." -Wilson Bentley. Author’s Web Site: http://www.jacquelinebriggsmartin.com/books/snowflak.html Simon, Seymour. (2007). Spiders. Collins. Other Seymour Simon Titles: Over 250 titles on a range of topics: Insects, Human Body, Author’s Web Site: http://www.seymoursimon.com/index.php Siy, Alexandra and Kunkel, Dennis. (2011). Bug shots: The good, the gad, and the bugly. Holiday House. Bug Shots features “rap sheets” and “wanted posters” of the world’s most wanted insects. Mug shots created from scientist Dennis Kunkel’s stunning electron micrographs provide the evidence for this larger than life entomological “case.” Author Alexandra Siy invites readers to join the FBI (by becoming Fellow Bug Investigators) and reach a verdict themselves: good, bad, or just plain bugly? The amazing photomicrographs magnify insect parts from 8 to more than 12,000 times their actual sizes providing close-up views of claws, jaws, eyes, antennae, wings, stingers, and other specialized parts. A detailed glossary and resource section compliment this fascinating ADVENTURES WITH A DIGITAL MICROSCOPE - DRAFT: Please Do Not Distribute book. Other Similar Alexandra Siy Titles: Mosquito Bites Author’s Web Site: http://www.alexandrasiy.com/ Van Allsburg, Chris. (1988). Two bad ants. Houghton Mifflin. Read the story of an ants’ point of view of the perils of the kitchen. Viewing the images reduces the reader to the size and perspective of an ant. Author’s Web Site: http://www.chrisvanallsburg.com/flash.html Wick, Walter. (1997). A drop of water: A book of science and wonder. Scholastic. Color photographs of water in various states and stages of movement capture moments of change in beautiful patterns. Photos include water drops; soap bubbles; water condensing and evaporating; snowflakes, frost, and dew; and water as a prism. There are activities for observing and experimenting with water. Author’s Web Site: http://www.walterwick.com/ Rogers, Kirsteen. (2006). The Usborne complete book of the microscope. London, UK: Usborne Publishing. Photographs and information about the microscopic world. Includes project ideas and Internet links to website. 22