Station 1: Bracket Fungi Found in woodlands where they are a major wood rotting group. Once a tree is infected, the fungus cannot be killed. They cause millions of dollars in damage through lost wood production and lumber decay. The only treatment may be to harvest younger trees before the rot spreads too far. Bracket fungi are usually hard and leathery or woodlike and have no stems. Woody shelves are a micro (small) habitat. They provide a unique place for animals to live. Spiders, mites, and insects live in large shelves. Some of the bracket or shelf fungi is used for herbal medicine. 1. What is the primary function of the bracket fungi? 2. What kinds of organisms use these fungi as a habitat? 3. What is the bracket fungi also called? Why do you think it has this name? Station 2: Do you like to eat yeast? There are about 160 species of single-celled fungi called yeasts. Some yeasts are used in baking, brewing and fermentation. Yeast does its work primarily by interacting with sugar and starches in either the dough of bread or liquid brewing and fermentation. Brewers’ yeast has long been considered nutritionally useful to humans because it contains a high quantity of B vitamins. Some yeasts can cause decay in fruits and vegetables. 1. How do we use yeast in our everyday lives? 2. Look at the microscope slide. It shows you how budding in yeast occurs. Draw what you see. What is budding an example of? 3. Use the hand lens to look at the yeast in the petri dish. How does it make the bread rise? Describe what you think happens. Budding yeast Station 3: Penicillin One of the most beneficial uses of a fungus came with the virtually accidental discovery of the antibiotic penicillin by Sir Alexander Fleming in 1928. Antibiotics, many of them derived from fungi, have helped revolutionize the practice of medicine in the 20th century. Alexander Fleming was studying deadly bacteria in 1928 when he made this dramatic discovery. For examination purposes, Fleming had removed the cover of the bacteria culture plate with which he was working. A mold formed on the exposed culture. Fleming noticed that in the area surrounding the mold, the bacteria had disappeared. He kept the strain of mold alive and began testing it on laboratory animals. In 1929 he published his first medical paper proving that a lowly mold from soil was a powerful microbe killer that did not injure human tissue. The mold was Penicillium notatum. 1. What did Alexander Fleming study? 2. How did Fleming discover the ‘miracle drug’? 3. Look at the microscope slide provided. It is of Penicillium. Can you see the hyphae and the spores? Draw what you see. Station 4: Molds: One chief difference between molds and other fungi is that molds, in their life cycles, alternate between animal and plant behavior. The fact that they are able to, at certain states of their lives, move about, gives them a similarity to protozoa, or tiny one-celled animals. Yet most of their functions and by their appearance, they resemble fungi. Most of them are saprophytic, obtaining their nutrition from dead organic matter such as meat, cheese, bread, fruits and vegetables. Some molds also produce mycotoxins that can pose serious health risks to humans and animals. Inhaling or touching mold or mold spores may cause allergic reactions or asthma attacks in sensitive people. Mold in the home can usually be found in damp, dark or steamy areas e.g. bathroom or kitchen, cluttered storage areas, recently flooded areas, basement areas, plumbing spaces, areas with poor ventilation and outdoors in humid environments. Symptoms caused by mold allergy are watery, itchy eyes, a chronic cough, headaches or migraines, difficulty breathing, rashes, tiredness, sinus problems, nasal blockage and frequent sneezing. 1. Look at the moldy bread with the hand lens. Can you see the hyphae (small filaments)? Can you see any spores? They look like tiny dots. 2. What is the meaning of saprophytic? 3. Why do some molds pose health risks to humans and animals? Station 5: Lichens: These are found on places like tree trunks, rocks and old boards. Each lichen consists of a fungus and an alga or cyanobacteria that live together so intimately that they resemble one organism. This is an example of a mutualistic relationship where both organisms benefit. The fungus makes up the bulk of the body. The algal and/or cyanobacterial partner(s) possess the green pigment chlorophyll, enabling them to use sunlight’s energy to make their own food from water and carbon dioxide through photosynthesis. They also provide vitamins to the fungus. Cyanobacteria can make amino acids directly from the nitrogen gas in the atmosphere, something neither fungi nor algae can do. The fungus, in turn, protects its partners from drying out and shades them from strong sunlight by enclosing the photosynthesizing partners within the body of the lichen. Lichens have a truly remarkable resistance to drought. A dry lichen can quickly absorb from 3 to 35 times its weight in water! Lichens can also absorb moisture from dew or fog, even from the air itself if the humidity is very high and the temperature is low. Lichens have many uses. Some are used to scent soaps and make perfumes. Others were used in the past to dye woolen cloth. The Lapp people, who live above the Arctic Circle in Scandinavia and Russia, harvest lichens as winter food for their reindeer, just like farmers in temperate zones stockpile hay. Sheep in the deserts of Libya survive, in part, by eating crustose lichens growing on rocks. Lichens are also important in making soil. Soil is made up of organic matter, such as decayed plants, and minerals. Species that grow on rocks infiltrate and wedge apart pieces of the rock by both pressure and chemical action. 1. How do lichens adapt to harsh conditions? 2. What does mutualistic mean? 3. Name the 2 parts a lichen consist of. 4. Name 3 uses of lichens. Station 6: Fungi: The Latin word for mushroom is ‘fungus’. The word stand for a whole group of organisms that do not contain chlorophyll and lack complex structures such as roots, stems, leaves and flowers. Included among the fungi are mushrooms, molds, yeasts, mildews, rusts, smuts and truffles. They get their food from other plants and animals. When they get it from living organisms, they are parasites. When they get it from dead organic matter, they are called saprophytes. The record price paid for a single white truffle was set in December 2007, when a casino owner paid 330,000 USD (£165,000) for a specimen weighing 1.5 kilograms (3.3 lb). Mildew 1. What is the difference between a parasite and a saprophyte? 2. What do the organisms in these pictures have in common? Corn smuts Truffle Station 7: Where can you find a fungus? Fungi live both on land and in the water. Fungi usually grow best in environments that are slightly acidic (a pH measurement of 5 or so; a pH of 7 is neutral). They can grow on substances with very low moisture. Fungi live in the soil and on your body, in your house and on plants and animals, in freshwater and seawater. A single teaspoon of topsoil contains about 120,000 fungi. Only a small portion of those that live on land is normally visible. Most of the fungus grows underground and form the mycelium which is a tangled mass of hyphae. The visible parts of the fungi vary greatly in size. Some are so tiny they cannot be seen without the aid of magnification. Others are quite large. In 1992 scientists announced the discovery of a giant underground fungus, Armillaria ostoyae, that covered 1,500 acres in Washington State. It is believed by most to be bigger than the 110-foot- (33.5-meter-) long, 200-ton blue whale. Based on its current growth rate, the fungus is estimated to be 2,400 years old but could be as ancient as 8,650 years, which would earn it a place among the oldest living organisms as well. The only visible signs of its existence were above ground mushrooms and a rot deadly to trees. 1. What is the mycelium? 2. Describe the habitat requirements for most fungi. The only sign of the Armillaria ostoyae above ground are these golden honey mushrooms. Station 8: Mycorrhizae: It is a symbiotic association between a fungus and the roots of a vascular plant. The plant provides carbohydrates made through photosynthesis to the fungus and the fungus helps with the absorptions of water and minerals out of the soil. The fungus also acts as a moist cushion against heat and drought. Both organisms benefit in this association. Research since these discoveries show that 90 percent of all green plants on land form these relationships. The fungal hairs are much finer than root hairs, and so have a much larger surface area compared to roots. They can grow into small places that plant roots cannot. 1. Describe 3 ways how the fungus help plants in this relationship. Plant on the left is without mycorrhizae. Plant on the right with mycorrhizae.