Unit IV: Cell Structure & Transport Part 1 – Cell Structure Hierarchy of Cell Structure Cells Organelles Mixtures Compounds Atoms/Elements Cell Discoveries… How a cell came to be called a “cell”… Cell Discoveries… Microscope man… Cell Theory 1. All living things are made of cells 2. All cells come from other cells Any exceptions? Where did the first cell come from? 3. Cells are the basic units of life Meaning? Cell Theory 1. All living things are made of cells 2. All cells come from other cells Schleiden – plants + Schwann – animals = ALL Virchow – cell reproduction 3. Cells are the basic units of life Meaning? Cell Structure - Typical Cell? Most cells contain same organelles and compounds, but, cells are NOT identical in structure (appearance) Cell Structure & Function The job fits the parts! This applies to shape of cell & organelles in it Structure and function go hand-in-hand for all parts of living things…including cells Depending on what the cell’s job, it must be structured differently than a “typical cell” For example… Neurons: carry electrical messages How related to its structure? Neurons: carry electrical messages - have many branches & are long…allows them to communicate better Muscle cells contract to cause movements How related to its structure? Muscle cells contract to cause movements - are very long…allows them to contract Respiratory lining cells have cilia (tiny hairs) How related to function? Respiratory lining cells have cilia (tiny hairs) - cilia move mucus along tube Cell Types: The Prokaryotic Cell No nucleus; single circular chromosome No membrane-bound organelles Ex: bacteria ~ cell wall (peptidoglycan) ~ some with flagella ~ no specific spot for DNA pro = first; before kary – kernel; nucleus Cell Types: The Eukaryotic Cell Has membrane-bound nucleus that holds DNA Has “fancy” organelles Usually larger in size Ex: everything EXCEPT bacteria Eu – good Cell Types: Plant Cell ~ cell wall (cellulose) ~ chloroplasts ~ large central vacuole ~ NO centrioles ~ NO cilia or flagella Cell Types: Animal Cell ~ NO cell wall ~ NO chloroplasts ~ small vacuoles ~ centrioles ~ may have cilia or flagella POP Quiz – Cell Structure 1. Which of the following are prokaryotic cells? A. plants B. animals C. fungi D. bacteria 2. If a particular cell has a cell wall, it is unlikely to contain A. centrioles B. ribosomes C. chloroplasts D. DNA POP Quiz – Cell Structure 3. Prokaryotic cells will NOT contain a A. cell wall B. cell membrane C. nucleus D. flagellum 4. Animal cells are easily distinguished from plants because they lack a A. cell wall B. cell membrane C. nucleus D. flagellum POP Quiz – Cell Structure 5. All of the following structures can be found in ALL cell types, EXCEPT A. cell membrane B. mitochondria C. DNA D. ribosomes 6. Schleiden’s contribution to the cell theory was that A. All animals are made of cells B. All cells come from preexisting cells C. All plants are made of cells D. None of the above POP Quiz – Cell Structure 7. Bob finds something in his yard and wonders if it is living or nonliving. Which of the following would be the best evidence that it is living? A. It moves B. It gives off heat C. It contains cells D. It gives off water 8. Which of the following is NOT a part in the cell theory? A. B. C. D. All organisms are made of cells Cells are all the same shape and size Cells are the basic unit of life All cells come from preexisting cells Endosymbiotic theory Cell Structures (refer to your cell diagrams) Cell wall Tough covering…provides structure & protection; animal cells do not have these Cell Structures (refer to your cell diagrams) Cell Membrane Lipid bilayer; controls cell homeostasis & transport Cytoplasm Water-based fluid; contains dissolved substances Cell Structures (refer to your cell diagrams) Cytoskeleton Fibers that form framework of cell Cell Structures (refer to your cell diagrams) Nucleus Membrane-bound… contains DNA; controls cell activities Nucleolus Spot within nucleus; deals with RNAs Cell Structures (refer to your cell diagrams) Endoplasmic Reticulum (ER) “highway system”; chemicals made by cell travel thru this Rough ER – holds ribosomes (makes proteins) Smooth ER – site of lipid synthesis Cell Structures (refer to your cell diagrams) Ribosomes Site of protein synthesis; may be “free” or bound to rough ER Cell Structures (refer to your cell diagrams) Golgi Apparatus “post office”; packages & sends out chemicals Flattened sacs stacked on each other Cell Structures (refer to your cell diagrams) Mitochondria Membranous; turns glucose into energy molecules (ATP) All eukaryotic cells have these Cell Structures (refer to your cell diagrams) Chloroplast Membranous; contains chlorophyll turns light & CO2 into glucose Cell Structures (refer to your cell diagrams) Vacuoles Storage vesicles Central vacuole – in plants; very large Cell Structures (refer to your cell diagrams) Lysosomes Contain digestive enzymes; “eat” substances Cell Structures (refer to your cell diagrams) Peroxisomes Contains enzymes that detoxify chemicals Cell Structures (refer to your cell diagrams) Centrioles Used to make fibers for mitosis Cell Structures (refer to your cell diagrams) Flagella Tail-like structure; moves cell Cilia Hair-like structures; move cell or fluids in cell environment Cell Structures (refer to your cell diagrams) Microvilli Finger-like extensions; increase surface area Endosymbiont Theory Mitochondria & chloroplasts are thought to have come from ancient prokaryotic cells Evidence: Both mitochondria and chlorplasts… have their own DNA have their own ribosomes have membranes similar to bacteria divide like bacteria (binary fission) Recap - Cell Structure Eukaryotic cells are much more complicated than prokaryotic cells, but all cells have: cell membrane, cytoplasm, DNA, ribosomes, & cytoskeleton What is so critical about each of these that ALL cells have them? Unit IV: Cell Structure & Transport Part 2 – Cell Transport & Homeostasis Cell Membrane phospholipids: the main ingredient of the cell membrane; they have polar side & nonpolar sides Which side of the molecule “likes” water? How does that relate to the way it must face? Cell Membrane: controls what goes in/out Structure: fluid-mosaic model molecules stick to each other, but able to move phospholipid bilayer: ~ polar (hydrophilic) …faces outward ~ nonpolar (hydrophobic) …faces inward non = not phil = love phob = fear bi - two Cell Membrane: controls what goes in/out Structure: integral proteins help with transport cholesterol keeps it fluid glycoproteins act as “ID tags” receptors react to chemicals Cell Membrane: controls what goes in/out What is the purpose of… Glycolipids & Glycoproteins? Cholesterol? Integral proteins? 12. What is the function of the cell membrane? 11 10 1 13. What are the three parts of the cell theory? 2 3 4 5 9 8 7 6 14. What is the function of the nucleus? 15. What is the difference between a prokaryote and eukaryote? Cell Transport Active Transport – requires energy; moves against concentration gradient (low to high) or involves large particles 1. endocytosis – “traps” stuff & brings in 2. exocytosis – releasing stuff to outside Cyto - cell Cell Transport Passive Transport – no energy required; moves with concentration gradient (high to low) Cell Transport Passive Transport 1. diffusion – move from high to low concentration until equilibrium is reached Cell Transport Passive Transport 2. osmosis- diffusion of water through selectively permeable membrane (like cell membrane) Typically, water can move freely in/out of membrane but solutes (like glucose) cannot Water moves to try to equal out the concentrations on both sides Hypotonic – lower concentration of solute Hypertonic – higher conc. of solute Ex: distilled water is pure water…it would be hypotonic to cells (and everything else!) Ex: the Dead Sea is very salty…it would be hypertonic to cells Isotonic – equal conc. of solute Osmosis: water moves to the side with more “stuff” What happens if a cell is placed in an environment that has a low solute concentration? Where is there more “stuff”? What will the result be? Hypo – below Osmosis: water moves to the side with more “stuff” What happens if a cell is placed in an environment that has a high solute concentration? Where is there more “stuff”? What will the result be? Hyper – above; over Osmosis: water moves to the side with more “stuff” What happens if a cell is placed in an environment that has an equal solute concentration? Where is there more “stuff”? What will the result be? iso – equal Cell Transport Passive Transport 3. facilitated diffusion- like diffusion, but with “helper” molecule (larger molecules like glucose need this)…still goes high to low The End LAB – Osmosis in Potato Cells I. PURPOSE How will potato cells react when placed in hypertonic or hypotonic solutions? II. BACKGROUND Hypertonic solutions have more “stuff” dissolved. Hypotonic solutions have less “stuff” dissolved. LAB – Osmosis in Potato Cells III. HYPOTHESES Potato cells in hypertonic solution will ___ Potato cells in hypotonic solution will ___ IV. PROCEDURE (draw pic) V. DATA SALT MASS (g) MASS (g) CONC (%) 0% (Hypo) 10% (Hyper) Initial Final MASS Difference LAB – Osmosis in Potato Cells VI. CONCLUSION What happened in set-up? Explain why it happened. Does this support OR not support your hypotheses. Describe 2 sources of error related to this lab. Highlight these in your notes… Passive Transport Diffusion Osmosis Facilitated diffusion Active transport Endocytosis Exocytosis Phospholipid bilayer Fluid mosaic Cholesterol Glycolipid (-protein) Channel/helper protein Integral protein Peripheral protein Highlight these in your notes… Prokaryotic Eukaryotic Endosymbiont theory Mitochondria Chloroplasts Cell theory Cell membrane Ribosome Cytoplasm DNA/chromosome Highlight these in your notes… ALL CELLS… Cell membrane Ribosome Cytoplasm DNA/chromosome Cell wall Flagella (cilia) EUKARYOTES only… Rough ER Smooth ER Golgi Mitochondria Chloroplast Lysosome Nucleus Quiz – Cells #2 Answer on your own paper. You do NOT need to write the questions. Quiz – Cells #2 1. Name the parts. Choices… helper protein – peripheral protein - phospholipid glycoprotein – glycoprotein - cholesterol Quiz – Cells #2 2. Describe the function of each part of the cell membrane. A. helper protein B. glycoproteins C. cholesterol Quiz – Cells #2 3. Why does it have to be a LIPID layer that makes up the cell membrane? 4. Why does it have to be a BILAYER (2 layers) for the cell membrane? 5. Which part “loves” water? A ………..or B Cell Size Limits What is the lower limit of cell size determined by? ~ is set by having enough DNA to code metabolism, do metabolism, and reproduce Cell Size Limits What is upper limit set by? Upper limit is set by surface area to volume ratio ~Must be enough plasma membrane (SA) to allow fast enough transport rate to supply entire cytoplasm (volume) with chemicals Cell Size Limits As cell enlarges, volume increases faster than surface area