Last of cell respiration and lab - FWScienceJohnson-Bio

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BW
1. Take out your science fair packet, and explain to
me how much you have done so far.
2. How many hours do you think you have put
into sci. fair so far?
3. What would you do if you were given another
week to work on it?
Bring a book to your seat
Friday the 13th-SCI FAIR
• Rough draft of:
-hypothesis
-procedures
-materials
-data table
-graph
-conclusion
*extra credit for bringing in board before break
Wed 12/4
-Re-do lesson on Cell Respiration and
Aerobic/Anaerobic
*Be able to fill in pink Graphic Organizer as
closure
-Do pre-lab for Yeast Lab (tomorrow)
(do NOT be absent!)
Step
1
Glycolysis
Where:
What:
NO Oxygen available:
Anaerobic respiration
Products:
Require O2?
ATP:
Kreb’s cycle
Where:
Products:
Require O2?
ATP:
2 types are:
Products:
Require O2?
ATP:
Step
2
What:
Fermentation
What: Pyruvate +NADH ATP
Alcoholic Fermentation
Where:
What:
Oxygen required:
Aerobic respiration
Lactic Acid Fermentation
Where:
What:
Products:
Require O2?
ATP:
Cellular Respiration: glucose ATP
Break down food to form energy
Step
3
Electron Transport
Chain (ETC)
Where:
What:
Products:
Require O2?
ATP:
Step
1
NO Oxygen available:
Anaerobic respiration
Glycolysis
Where: cytoplasm
What: Break down glucose into
pyruvate and ATP
Products: Pyruvate, NADH
Require O2? NO
ATP: 2
2 types are:
NAD+
Require O2? NO
ATP: 2
Step
2
Kreb’s cycle
Where: Matrix of
mitochondria
What: Breakdown of pyruvate
Products: CO2, NADH, FADH2
Require O2? Sort of
ATP: 2
Fermentation
What: Pyruvate +NADH ATP
Alcoholic Fermentation
Where: Yeast (fungi)
What: PyruvateATP
Products: CO2, ethanol,
Oxygen required:
Aerobic respiration
Lactic Acid Fermentation
Where: muscle cells (animals)
What: PyruvateATP
Products: Lactic Acid, NAD+
Require O2? NO
ATP: 2
Cellular Respiration: glucose ATP
Break down food to form energy
Step
3
Electron Transport
Chain (ETC)
Where: inner
membrane of mitochondria
What: NADH, FADH2  ATP
Products: H20, NAD+
Require O2? YES!
ATP: 32
Aerobic VS. Anaerobic
-Requires O2
-Produces 36
ATP from 1
glucose.
-Happens in
cytoplasm
&mitochondria
-3phases
-does NOT
require O2
-produces 2 ATP
from 1 glucose
-happens in
muscle
-Yeast does it
- 2 types of
fermentation
Make a Table
Process
Glycolysis
Krebs Cycle
Electron
Transport
Chain
Lactic Acid
Fermentation
Alcoholic
Fermentation
Cell
Reactants
Location
Products
ATP
Produced
Oxygen
Required
?
*blue= not used up (not ‘real’ reactant) but important electron carriers!
Process
Cell
Location
Reactant
s
Glucose
Products
ATP
Produced
Oxygen
Required
?
Pyruvate
NADH
2
NO
CO2
2
Yes
Glycolysis
Cytoplasm
Krebs Cycle
Mitochondira Pyruvate
matrix
Electron
Transport
Chain
Mitochrondira
inner
membrane
O2
NADH
FADH
H20
32
Yes
Lactic Acid
Fermentatio
n
Muscle Cells
Pyruvate
NADH
Lactic
Acid
NAD+
2
NO
Alcoholic
Fermentatio
n
Yeast,
Bacteria
Pyruvate
NADH
Alcohol
CO2
NAD+
2
NO
Fill in key terms sheet
Bellwork
Pass your lab report up & to the left! (see board for the order to
staple pages)
1.
What were 2 of your “Rules of Life”
from yesterday?
2.
Humans use yeast every day. What is
yeast, and what are some common uses
of yeast?
3.
You can buy yeast to make bread in the
grocery store. This yeast consists of
little brown grains. Do you think that
these little brown grains of yeast are
alive? Why or why not?
4.
Finish updating your table of
contents!!!
Yeast
To be or not to be…
• There are 5 characteristics of all living
things (organisms):
• 1. Organization (cells)
• 2. Metabolism (use energy/make waste)
• 3. Grow and develop
• 4. Reproduce
• 5. Respond and adapt
Yeast Lab
• Problem Statement/Question:
• Is yeast alive?
Decide Who’s Who
• Jefe- reads instructions, keeps everyone on task, keeps
track of time limits (use a cell phone stopwatch)
• Recorder- Makes sure everyone writes down the correct
information, makes sure everyone is listening
• Presenter- answers teacher’s Qs, encourages all members
to speak up and participate during lab
• Materials Manager- gets & puts away all materials in
correct location, makes sure everyone treats materials with
respect
Yeast Lab
• Problem Statement/Question:
• Is yeast alive?
Presenter: read introduction on lab
sheet
Yeast Lab
• Problem Statement/Question:
• Is yeast alive?
• Hypothesis:
• Write an If…then…as measured by…hypothesis
(we will be measuring to see if yeast produces a
gas as a metabolic waste product)
Jefe: take group to NEW lab table to
read procedures & assign tasks
Materials person: collect materials
Finish Key Terms
• Add these 4 terms
1. Aerobic
2. Anaerobic
3. Lactic Acid Fermentation
4. Alcoholic Fermentation
• Use your Notes! (and
complete your table if you
haven’t already)
Cellular Respiration Key Terms
1. C6H12O6
2. O2
3. CO2
4. ATP
5. Autotroph
6. Heterotroph
7. Cellular Respiration
8. Mitochondrion(a)
9. Cristae
10. matrix
11. glycolysis
12. Calvin Cycle
13. Krebs cycle
14. Electron Transport Chain
15. Aerobic
16. Anaerobic
17. Lactic Acid Fermentation
18. Alcoholic Fermentation
Closure
• If a strange new sample was brought back
from Mars, it may look like nothing we’ve ever
seen before. Explain briefly how you would
test to see if it was a living organism!
BW
1. If yeast is alive, what does it need to survive?
2. Why might yeast be ‘dormant’ now and ready
to do ‘work’ when we add water?
3. What parts of cell respiration create CO2?
4. What parts of cell respiration occur in the
mitochondria?
5. What are the byproducts of alcoholic
fermentation?
Friday the 13th-SCI FAIR
• Rough draft of:
-hypothesis
-procedures
-materials
-data table
-graph
-conclusion
*extra credit for bringing in board before break
Thurs 12/5
-Review cell respiration (fill in table from
memory)
-Brainstorm what to change in lab report. You
can choose to follow given procedure or
change something.
-during experiment, work on filling in table
with partners, tan worksheet, key terms
sheet, and notecard (for test tomorrow)
Step
1
Glycolysis
Where:
What:
NO Oxygen available:
Anaerobic respiration
Products:
Require O2?
ATP:
Kreb’s cycle
Where:
Products:
Require O2?
ATP:
2 types are:
Products:
Require O2?
ATP:
Step
2
What:
Fermentation
What: Pyruvate +NADH ATP
Alcoholic Fermentation
Where:
What:
Oxygen required:
Aerobic respiration
Lactic Acid Fermentation
Where:
What:
Products:
Require O2?
ATP:
Cellular Respiration: glucose ATP
Break down food to form energy
Step
3
Electron Transport
Chain (ETC)
Where:
What:
Products:
Require O2?
ATP:
Yeast
Lab
Background info: I tried the experiment 4 times
using the given procedures, and it did not make
the balloon rise. (other teachers too)
Problem statement: Does something in the
procedures affect the production of CO2 or the
amount needed to fill the balloon?
Hypothesis: If we change one of the factors
affecting the yeast growth, then it will produce
enough CO2 to fill the balloon and it will stand
up, as measured by diameter of balloon.
YEAST
Check our variables:
The yeast (amount each group get has to be constant)
The sugar (not too much or too little)
The temp. (not too hot or too cold)
The balloon
The container
The time
YEAST
• Are alive so….
Want food….but not too much….or too little.
Will do aerobic, then anerobic respiration (both
of which release CO )
Like warmth (body temp), but not too hot.
If we warm the experiment, the reaction will
take place faster.
2
Alteration of procedures
• Do you and your group want to change any of
the procedures?
• You are still required to have at least 1 control
group (no sugar), but you can change other
factors: If you do, alter your procedures and
get them checked off by me. (Remember to do
the math)
Yeast Lab
• Problem Statement/Question:
• Is yeast alive?
Presenter: read introduction on lab
sheet
Jefe: take group to lab table to read
procedures & assign tasks
Materials person: collect materials
Finish Key Terms
• Add these 4 terms
1. Aerobic
2. Anaerobic
3. Lactic Acid Fermentation
4. Alcoholic Fermentation
• Use your Notes! (and
complete your table if you
haven’t already)
Cellular Respiration Key Terms
1. C6H12O6
2. O2
3. CO2
4. ATP
5. Autotroph
6. Heterotroph
7. Cellular Respiration
8. Mitochondrion(a)
9. Cristae
10. matrix
11. glycolysis
12. Calvin Cycle
13. Krebs cycle
14. Electron Transport Chain
15. Aerobic
16. Anaerobic
17. Lactic Acid Fermentation
18. Alcoholic Fermentation
Closure
• Groups that changed something, did it work to
make the CO2 raise the balloon?
• What could we try if we did this experiment
again?
To be or not to be…
• There are 5 characteristics of all living
things (organisms):
• 1. Organization (cells)
• 2. Metabolism (use energy/make waste)
• 3. Grow and develop
• 4. Reproduce
• 5. Respond and adapt
Yeast Lab
• Problem Statement/Question:
• Is yeast alive?
Decide Who’s Who
• Jefe- reads instructions, keeps everyone on task, keeps
track of time limits (use a cell phone stopwatch)
• Recorder- Makes sure everyone writes down the correct
information, makes sure everyone is listening
• Presenter- answers teacher’s Qs, encourages all members
to speak up and participate during lab
• Materials Manager- gets & puts away all materials in
correct location, makes sure everyone treats materials with
respect
Yeast Lab
• Problem Statement/Question:
• Is yeast alive?
• Hypothesis:
• Write an If…then…as measured by…hypothesis
(we will be measuring to see if yeast produces a
gas as a metabolic waste product)
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