BW 1. Take out your science fair packet, and explain to me how much you have done so far. 2. How many hours do you think you have put into sci. fair so far? 3. What would you do if you were given another week to work on it? Bring a book to your seat Friday the 13th-SCI FAIR • Rough draft of: -hypothesis -procedures -materials -data table -graph -conclusion *extra credit for bringing in board before break Wed 12/4 -Re-do lesson on Cell Respiration and Aerobic/Anaerobic *Be able to fill in pink Graphic Organizer as closure -Do pre-lab for Yeast Lab (tomorrow) (do NOT be absent!) Step 1 Glycolysis Where: What: NO Oxygen available: Anaerobic respiration Products: Require O2? ATP: Kreb’s cycle Where: Products: Require O2? ATP: 2 types are: Products: Require O2? ATP: Step 2 What: Fermentation What: Pyruvate +NADH ATP Alcoholic Fermentation Where: What: Oxygen required: Aerobic respiration Lactic Acid Fermentation Where: What: Products: Require O2? ATP: Cellular Respiration: glucose ATP Break down food to form energy Step 3 Electron Transport Chain (ETC) Where: What: Products: Require O2? ATP: Step 1 NO Oxygen available: Anaerobic respiration Glycolysis Where: cytoplasm What: Break down glucose into pyruvate and ATP Products: Pyruvate, NADH Require O2? NO ATP: 2 2 types are: NAD+ Require O2? NO ATP: 2 Step 2 Kreb’s cycle Where: Matrix of mitochondria What: Breakdown of pyruvate Products: CO2, NADH, FADH2 Require O2? Sort of ATP: 2 Fermentation What: Pyruvate +NADH ATP Alcoholic Fermentation Where: Yeast (fungi) What: PyruvateATP Products: CO2, ethanol, Oxygen required: Aerobic respiration Lactic Acid Fermentation Where: muscle cells (animals) What: PyruvateATP Products: Lactic Acid, NAD+ Require O2? NO ATP: 2 Cellular Respiration: glucose ATP Break down food to form energy Step 3 Electron Transport Chain (ETC) Where: inner membrane of mitochondria What: NADH, FADH2 ATP Products: H20, NAD+ Require O2? YES! ATP: 32 Aerobic VS. Anaerobic -Requires O2 -Produces 36 ATP from 1 glucose. -Happens in cytoplasm &mitochondria -3phases -does NOT require O2 -produces 2 ATP from 1 glucose -happens in muscle -Yeast does it - 2 types of fermentation Make a Table Process Glycolysis Krebs Cycle Electron Transport Chain Lactic Acid Fermentation Alcoholic Fermentation Cell Reactants Location Products ATP Produced Oxygen Required ? *blue= not used up (not ‘real’ reactant) but important electron carriers! Process Cell Location Reactant s Glucose Products ATP Produced Oxygen Required ? Pyruvate NADH 2 NO CO2 2 Yes Glycolysis Cytoplasm Krebs Cycle Mitochondira Pyruvate matrix Electron Transport Chain Mitochrondira inner membrane O2 NADH FADH H20 32 Yes Lactic Acid Fermentatio n Muscle Cells Pyruvate NADH Lactic Acid NAD+ 2 NO Alcoholic Fermentatio n Yeast, Bacteria Pyruvate NADH Alcohol CO2 NAD+ 2 NO Fill in key terms sheet Bellwork Pass your lab report up & to the left! (see board for the order to staple pages) 1. What were 2 of your “Rules of Life” from yesterday? 2. Humans use yeast every day. What is yeast, and what are some common uses of yeast? 3. You can buy yeast to make bread in the grocery store. This yeast consists of little brown grains. Do you think that these little brown grains of yeast are alive? Why or why not? 4. Finish updating your table of contents!!! Yeast To be or not to be… • There are 5 characteristics of all living things (organisms): • 1. Organization (cells) • 2. Metabolism (use energy/make waste) • 3. Grow and develop • 4. Reproduce • 5. Respond and adapt Yeast Lab • Problem Statement/Question: • Is yeast alive? Decide Who’s Who • Jefe- reads instructions, keeps everyone on task, keeps track of time limits (use a cell phone stopwatch) • Recorder- Makes sure everyone writes down the correct information, makes sure everyone is listening • Presenter- answers teacher’s Qs, encourages all members to speak up and participate during lab • Materials Manager- gets & puts away all materials in correct location, makes sure everyone treats materials with respect Yeast Lab • Problem Statement/Question: • Is yeast alive? Presenter: read introduction on lab sheet Yeast Lab • Problem Statement/Question: • Is yeast alive? • Hypothesis: • Write an If…then…as measured by…hypothesis (we will be measuring to see if yeast produces a gas as a metabolic waste product) Jefe: take group to NEW lab table to read procedures & assign tasks Materials person: collect materials Finish Key Terms • Add these 4 terms 1. Aerobic 2. Anaerobic 3. Lactic Acid Fermentation 4. Alcoholic Fermentation • Use your Notes! (and complete your table if you haven’t already) Cellular Respiration Key Terms 1. C6H12O6 2. O2 3. CO2 4. ATP 5. Autotroph 6. Heterotroph 7. Cellular Respiration 8. Mitochondrion(a) 9. Cristae 10. matrix 11. glycolysis 12. Calvin Cycle 13. Krebs cycle 14. Electron Transport Chain 15. Aerobic 16. Anaerobic 17. Lactic Acid Fermentation 18. Alcoholic Fermentation Closure • If a strange new sample was brought back from Mars, it may look like nothing we’ve ever seen before. Explain briefly how you would test to see if it was a living organism! BW 1. If yeast is alive, what does it need to survive? 2. Why might yeast be ‘dormant’ now and ready to do ‘work’ when we add water? 3. What parts of cell respiration create CO2? 4. What parts of cell respiration occur in the mitochondria? 5. What are the byproducts of alcoholic fermentation? Friday the 13th-SCI FAIR • Rough draft of: -hypothesis -procedures -materials -data table -graph -conclusion *extra credit for bringing in board before break Thurs 12/5 -Review cell respiration (fill in table from memory) -Brainstorm what to change in lab report. You can choose to follow given procedure or change something. -during experiment, work on filling in table with partners, tan worksheet, key terms sheet, and notecard (for test tomorrow) Step 1 Glycolysis Where: What: NO Oxygen available: Anaerobic respiration Products: Require O2? ATP: Kreb’s cycle Where: Products: Require O2? ATP: 2 types are: Products: Require O2? ATP: Step 2 What: Fermentation What: Pyruvate +NADH ATP Alcoholic Fermentation Where: What: Oxygen required: Aerobic respiration Lactic Acid Fermentation Where: What: Products: Require O2? ATP: Cellular Respiration: glucose ATP Break down food to form energy Step 3 Electron Transport Chain (ETC) Where: What: Products: Require O2? ATP: Yeast Lab Background info: I tried the experiment 4 times using the given procedures, and it did not make the balloon rise. (other teachers too) Problem statement: Does something in the procedures affect the production of CO2 or the amount needed to fill the balloon? Hypothesis: If we change one of the factors affecting the yeast growth, then it will produce enough CO2 to fill the balloon and it will stand up, as measured by diameter of balloon. YEAST Check our variables: The yeast (amount each group get has to be constant) The sugar (not too much or too little) The temp. (not too hot or too cold) The balloon The container The time YEAST • Are alive so…. Want food….but not too much….or too little. Will do aerobic, then anerobic respiration (both of which release CO ) Like warmth (body temp), but not too hot. If we warm the experiment, the reaction will take place faster. 2 Alteration of procedures • Do you and your group want to change any of the procedures? • You are still required to have at least 1 control group (no sugar), but you can change other factors: If you do, alter your procedures and get them checked off by me. (Remember to do the math) Yeast Lab • Problem Statement/Question: • Is yeast alive? Presenter: read introduction on lab sheet Jefe: take group to lab table to read procedures & assign tasks Materials person: collect materials Finish Key Terms • Add these 4 terms 1. Aerobic 2. Anaerobic 3. Lactic Acid Fermentation 4. Alcoholic Fermentation • Use your Notes! (and complete your table if you haven’t already) Cellular Respiration Key Terms 1. C6H12O6 2. O2 3. CO2 4. ATP 5. Autotroph 6. Heterotroph 7. Cellular Respiration 8. Mitochondrion(a) 9. Cristae 10. matrix 11. glycolysis 12. Calvin Cycle 13. Krebs cycle 14. Electron Transport Chain 15. Aerobic 16. Anaerobic 17. Lactic Acid Fermentation 18. Alcoholic Fermentation Closure • Groups that changed something, did it work to make the CO2 raise the balloon? • What could we try if we did this experiment again? To be or not to be… • There are 5 characteristics of all living things (organisms): • 1. Organization (cells) • 2. Metabolism (use energy/make waste) • 3. Grow and develop • 4. Reproduce • 5. Respond and adapt Yeast Lab • Problem Statement/Question: • Is yeast alive? Decide Who’s Who • Jefe- reads instructions, keeps everyone on task, keeps track of time limits (use a cell phone stopwatch) • Recorder- Makes sure everyone writes down the correct information, makes sure everyone is listening • Presenter- answers teacher’s Qs, encourages all members to speak up and participate during lab • Materials Manager- gets & puts away all materials in correct location, makes sure everyone treats materials with respect Yeast Lab • Problem Statement/Question: • Is yeast alive? • Hypothesis: • Write an If…then…as measured by…hypothesis (we will be measuring to see if yeast produces a gas as a metabolic waste product)