AP Lab 7 Wisconsin Fast Plants “Who’s The Father? What are Wisconsin Fast Plants? FIRST: Read the lab and do p.2 • Type of mustard, radish, cabbage plant especially bred by Paul Williams of the University of Wisconsin WFP Life Cycle Video • WFP Time Lapse Video (scroll below life cycle diagram) See stems:PP or Pp (purple) and pp (non-purple or green) Standard (Tall) TT Dwarf (tt) Grow seeds in Petri dish Use Lid as bottom Other part as cover Cut paper towel circles • Wet paper towel well with a pipette NAME FRIDAY –May 15 Work in Pairs 2 Petri dishes/table -Mark Petri dish lid into two halves -Label P on one side, F1 the other -Moisten paper towel circle well and place 5 seeds in each side of the lid Put you initials F2 WFP (1:57) P F1 on the Petri dish cover 2 cm water seeds above water line tilt dish slightly name on Petri dish Petri dish at slight angle under a light It will sit all week-end so be sure there is enough water in the Petri dish. Water is to break the seed coat Germinates in a day or two Root emerges with root hairs to increase surface area for water absorption. Day4 (MONDAY) (cont. on next slide) • Stem and leaves emerge • Water F1 and P well. • “Observing the Seeds” (make 3 quantitative and 3 qualitative observations, sketch and color, count the genotypes and phenotypes.) • Can also take a picture. SOME TRAITS • • • • • Non-purple (green) – recessive pp Purple - dominant PP or Pp Green – dominant pp Tall – dominant T Dwarf- recessive t Clean out 1 Petri dish Label F2 Save F1/P1 Petri Dish Tuesday: Now plant F2 • • • • • • • • Place 10 seeds in a Petri dish 0 1 per table 0 Two rows of 5 0 Water well. 0 Label F2 0 Cover Label your lab table and period Put under light 0 0 0 0 0 Day 7 (Friday) p. 6 • DATA COLLECTION DAY OF F2 Purple Tall Green Tall Purple Dwarf Green Dwarf (see pictures next slide) Monday: ALL CLASS TOTALS OF F2 Purple/standard stem Non-purple/Standard stem Rosette dwarf Collect Group Data • P. 6 • Sketch plants, color • Do dihybrid of the plant genotypes Chi square Test • Bozeman Biology Chi Square Test • To test your null hypothesis • Critical value and degrees of freedom Day14-20 (flowers) How do you grow WFP? • Need constant light How do you grow WFP? • Seeds are tiny-do not cover with very much potting soil How do you grow WFP? • Need fertilizer Label your quads Green and Non-green leaves • Wisconsin Fast Plants® F1 Non-Purple Stem, Yellow-Green Leaf (F1 Anthocyaninless, Yellow-Green) • F1 seed from cross of Non-Purple Stem, Yellow-Green Leaf (anl/ygr) × Purple Stem (ANL/YGR);