Successfully Applying Bioremediation at Upstream,
Midstream, Downstream and Retail Sites
2015 Presentation
PRESENTER: Eric Bueltel, P.E.
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
2
Roadmap
ETEC, LLC - Recirculation-Based Remediation Strategies

Introduction:

ETEC, LLC
Bioremediation technologies
 Applications
 Role in cleanup


ETEC Approach to Bioremediation:




Delivery
Mass balance
Data evaluation & treatment modifications
Onsite assistance
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Roadmap Continued
3
ETEC, LLC - Recirculation-Based Remediation Strategies

Upstream Case Study:


Midstream Case Study:


Emergency Response at pipeline rupture
Downstream Case Study:


Landfarming for soil treatment
Surfactant recirculation for LNAPL recovery
Retail Case Study:


Groundwater recirculation at fueling station
Air sparge enhancement at fueling station
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
4
Introduction
ETEC, LLC - Recirculation-Based Remediation Strategies

ETEC, LLC
Providing bioremediation services since 1991
 Started landfarming Exxon Valdez shoreline soil
 Been providing in situ treatments since 1997
 Projects throughout North America include:

 Upstream
 Midstream
 Downstream
 Retail
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
5
Introduction - Technologies
ETEC, LLC - Recirculation-Based Remediation Strategies

For petroleum:
In situ Treatment (DO-ITTM)
 Super-OxTM oxygenation equipment
 PetroBacTM biological products & CBNTM Nutrients
 PetroSolvTM biosurfactant
 Dissolved Oxygen

For chlorinated solvents
Situ Delivery (ISDTM) groundwater recirculation
equipment
 CarBstrateTM nutrient-amended, soluble substrate
 In
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Introduction - Applications
6
ETEC, LLC - Recirculation-Based Remediation Strategies

For petroleum:
Landfarming
 Excavation applications
 Surfactant-flushing
 Groundwater recirculation with bioremediation
 Air sparge / biosparge enhancement
 Direct injection (via wells or DPT)


For chlorinated solvents:
Groundwater recirculation with bioremediation
 Direct Injection (via wells or DPT)

E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Introduction – Role in Cleanups
7
ETEC, LLC - Recirculation-Based Remediation Strategies

ETEC’s Cleanup Role

To assist environmental consultants with remediation
 Provide
in-depth, engineering-based proposals
 Guide consulting partners through the approval process
 Help with system design
 Install, startup and train consulting partners
 Assist with onsite injection labor (if requested)
 Provide all parts, pieces & maintenance if necessary
 Evaluate data for help in report writing
 Recommend treatment modifications
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Introduction – Role in Cleanups
8
ETEC, LLC - Recirculation-Based Remediation Strategies

ETEC’s Cleanup Role is not

To be remediation consultants
 Do not
perform site assessments
 Do not sample
 Do not write work plans
 Do not perform onsite injection (unless upon request)
 Do not carry out routine equipment maintenance
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
ETEC Approach to Bioremediation
9
ETEC, LLC - Recirculation-Based Remediation Strategies

It’s All About Delivery!

All applications are designed with contact in mind
 Products


are 100% soluble
No particles
No viscosity
 Applications

have adequate infrastructure
ETEC has experience with creative infrastructure
 Groundwater
recirculation is the best way to achieve sitewide in situ delivery
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
ETEC Approach to Bioremediation
10
ETEC, LLC - Recirculation-Based Remediation Strategies

It’s All About Delivery!

All applications are designed with contact in mind
 Products


are 100% soluble
No particles
No viscosity
 Applications

have adequate infrastructure
ETEC has experience with creative infrastructure
 Groundwater
recirculation is the best way to achieve sitewide in situ delivery
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
ETEC Approach to Bioremediation
11
ETEC, LLC - Recirculation-Based Remediation Strategies

Mass Balance - Petroleum

Biological product applications are contaminant mass based
A 1,000 lbs. hydrocarbon release

Using contaminant mass for calculating nutrient needs


Breakdown of 1,000 lb. of TPH requires



200 lb. nitrogen
50 lb. phosphorus
Using contaminant mass for calculating electron acceptor needs

Breakdown of 1,000 lb. of TPH requires


3,000 lb. oxygen
5,000 lb. nitrate/sulfate
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
ETEC Approach to Bioremediation
12
ETEC, LLC - Recirculation-Based Remediation Strategies

Surfactant-flushing applications are volume based
 Ideally 0.25 to
2 pore volumes
 Extraction volume can be 1.5 to 2 times injectate volume

Mass Balance – Chlorinated Solvents

Surfactant-flushing applications are mass based
 Overcome
natural electron acceptors
 Electron acceptor, ORP & TOC dependent
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
ETEC Approach to Bioremediation
13
ETEC, LLC - Recirculation-Based Remediation Strategies

Data Evaluation

Evaluate physical, chemical & biological data






Flow rates
Nutrients
Electron acceptors
ORP, pH
Bacterial counts
Treatment Modification

Provide partners with




Product application increases / decreases
Focus areas
Additional infrastructure
Operational modifications
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
ETEC Approach to Bioremediation
14
ETEC, LLC - Recirculation-Based Remediation Strategies

Onsite Assistance – As Requested

Bioremediation is work!
 Provide
equipment installation
 Perform periodic equipment maintenance
 Retrofit existing remediation systems
 Onsite biological product injections
 Onsite surfactant-flushing
 Landfarming services
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Upstream Case Study
15
ETEC, LLC - Recirculation-Based Remediation Strategies

Landfarming of Crude Impacted Soil

Crude oil spill, southern California
 Initial concentration
– 2,700 mg/kg
 Volume – 40,000 yards
 Cleanup goal - < 500 mg/kg
 Silty sand
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Upstream Case Study
16
ETEC, LLC - Recirculation-Based Remediation Strategies
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Upstream Case Study
17
ETEC, LLC - Recirculation-Based Remediation Strategies

Landfarming Application

Biological products used
 2,240 gallons
active microbial cultures
 6,500 lbs. nutrients

Activities
 Cell construction
 Soil
screening
 Tilling once per week
 Water application
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Upstream Case Study
18
ETEC, LLC - Recirculation-Based Remediation Strategies

Landfarming Results

After 3 months treatment
 TPH
soil concentrations were 200 mg/kg
 Cost with tilling - ~$10/cubic yard
 Order of magnitude savings over disposal
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
19
Midstream Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies

Excavation Application

Pipeline Rupture, central Florida
 Diesel
fuel release – aerial, surface & subsurface
 Very large excavation
 Emergency application – 48-hour mobilization
 Free-product in excavation bottom
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Midstream Case Study
20
ETEC, LLC - Recirculation-Based Remediation Strategies

Landfarming Application

Biological products used
 750 gallons
active microbial cultures
 4,000 lbs. nutrients

Activities
 Product
Mixing
 Application to pit bottom & sidewalls
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Midstream Case Study
21
ETEC, LLC - Recirculation-Based Remediation Strategies
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Midstream Case Study
22
ETEC, LLC - Recirculation-Based Remediation Strategies

Excavation Application Results

One Quarter Following Treatment
 All installed wells
below regulatory goals
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Downstream Case Study
23
ETEC, LLC - Recirculation-Based Remediation Strategies

Surfactant-flushing Application

Oil spill, eastern New Jersey
 35 feet
x 35 feet
 Up to 3.0 feet free-product oil
 Cleanup goal - < 0.01 feet free-product
 Silty sand
 DTW 5-8 feet bgs
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Downstream Case Study
24
ETEC, LLC - Recirculation-Based Remediation Strategies
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
25
Downstream Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies

Surfactant-flushing Application

Surfactant products used
 8,000 gallons

of 5% surfactant solution
Activities
 Injected
8,000 gallons of surfactant solution
 Extracted 15,000 gallons of groundwater
 Injected 1 pore volume / extracted 2 pore volumes
 1 week injection/extraction + 1 week extraction
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
26
Downstream Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies

Surfactant-flushing Application

After 3 months treatment
 No
LNAPL was detected
 Closure monitoring activities ongoing for NFA status
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
27
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies

Dissolved Oxygen In situ TreatmentTM (DO-IT)

Former fueling station, southern Virginia
 250 feet
x 100 feet
 Free-product present
 35,000 ppb benzene in MW-13
 Benzene contaminated groundwater entering creek
 Cleanup goal - < 50 µg/L in creek
 Silty sand with silt/clay confining horizons
 DTW 13-18 feet bgs
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Surfactants & Chemical Oxidants
Oxygenated Water
Nutrients
Substrates (Electron Donors)
Roadmap
REMEDIATION SUCCESS DEPENDS ON:
1. Physical Contact
1) It’s All About Delivery!!! Why automated
systems provide
2. equipment
Mass Balance
powerful delivery platforms to support site-wide biological degradation of
target contaminants.
APPLICATION/ENGINEERING
2) Fundamentals of Enhanced In Situ Bioremediation:
DIRECT
An overview
of the laws that govern biological
CONTACT
degradation of organic compounds.
SCIENCE
3) Case Studies: Field applications of the
technology/approach.
RESULTS
28
29
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies

Super-OxTM Oxygenation Equipment

Specifications








10-gpm recirculation rate
45-ppm dissolved oxygen process water
Metering for biological products, nutrients, & surfactants
Up to 12 customizable injection areas
Up to 6 integrated groundwater pumps
Activated carbon pre-treatment controls
Fully weatherized 8’x10’ building
220V 100-amp
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Retail Case Study
30
ETEC, LLC - Recirculation-Based Remediation Strategies

Super-OxTM Oxygenation Equipment
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Bioremediation Treatments
31
ETEC, LLC - Recirculation-Based Remediation Strategies
GasSuper-Ox
Station ™
systems provide:
24/7 electron
acceptor &
amendment
delivery
UST
UST
Optimum
contact via pore
volume
exchanges
Managed in situ
environment for
accelerated
microbial
activity
Hydraulic
capture and
plume control
Extraction well
Bedrock
Aerobic Bioremediation
32
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies
GW BTEX: Freeproduct gasoline
(MW-33, MW-34)
GW BENZENE:
35,000 ppb
(MW-13)
LITHOLOGY:
silty sand with semiconfining silt/clay
horizons
DTW: 13-18’ bgs
Injection Area #3
Injection
Area #1
PLUME: 250’ x 100’
GOALS:
BTEX/Benzene
reductions
INFRASTRUCTURE:
Existing MPE
Network
Injection
Area #2
12 Injection wells
2 Extraction wells
Aerobic Bioremediation
Retail Case Study
33
ETEC, LLC - Recirculation-Based Remediation Strategies

Groundwater Recirculation – Case Study

System operation



TPH Degradation:




24-Month system operation
Electron acceptor delivery:
 3 million gallons of water @ 45 ppm DO = 1,200 lbs. of O2 from Super-OxTM
 1,000 lbs. Initial + 500 lbs. x 23 mo. = 12,500 lbs. CBN™ (7,500 lbs. NO3-SO4)
Aerobic degradation via DO = 400 lbs.
Anaerobic degradation via nitrate/sulfate = 1,500 lbs.
TOTAL: 2,900 lbs. in 24 months
Costs:


Super-OxTM Installation & Rental: $72,000
Amendment Costs: $61,000
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
ETEC
Bioremediation
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies
MW-34
100,000
90,000
18
Startup
Shutdown
80,000
Restart
20
Axis Title
70,000
60,000
22
50,000
40,000
24
BTEX
DTW
30,000
20,000
26
10,000
0
28
Axis Title
Retail Case Study
35
ETEC, LLC - Recirculation-Based Remediation Strategies
Injection Area #3
Injection
Area #1
Injection
Area #2
Aerobic Bioremediation
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies
MW-13
40,000
35,000
14
Startup
Shutdown
Restart
16
30,000
18
Axis Title
25,000
20,000
20
BTEX
15,000
22
10,000
24
5,000
0
26
Axis Title
DTW
37
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies
GW BTEX: Freeproduct gasoline
(MW-33, MW-34)
GW BENZENE:
35,000 ppb
(MW-13)
LITHOLOGY:
silty sand with semiconfining silt/clay
horizons
DTW: 13-18’ bgs
Injection Area #3
Injection
Area #1
PLUME: 250’ x 100’
GOALS:
BTEX/Benzene
reductions
INFRASTRUCTURE:
Existing MPE
Network
Injection
Area #2
12 Injection wells
2 Extraction wells
Aerobic Bioremediation
38
Retail Case Study
ETEC, LLC - Recirculation-Based Remediation Strategies

Observations from the Treatment

MW-34
 99%

BTEX reduction
MW-13
 92%
BTEX reduction
 98% benzene reduction
Appeased stakeholder with minimal disturbance
 Stream locations were below 50 ppb benzene in 24
months

E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
Aerobic
Bioremediation
39
Summary
ETEC, LLC - Recirculation-Based Remediation Strategies
Eric Bueltel, P.E.
Project Manager - Colorado
ETEC, LLC
971-222-3616 x104
eric@etecllc.com
www.etecllc.com/movie
E-MAIL: eric@etecllc.com
PHONE: 971-222-3616 x104
Aerobic
Bioremediation