Rat dissection labs start next week and continue for three weeks total (Labs 10-12) Dissection kits are provided (check your drawers now), but you are welcome to bring your own kit Lab 9: Animals III: Deuterostomes TODAY YOU HAVE A HAND IN FOR YOUR EXIT ACTIVITY Task C-2 today does NOT deal with echinoderms and chordates… Let’s do it first! Task C-2: Invertebrate keying • work as a pair • get one of the invertebrate keying boxes • use the dichotomous key (Table 9-1) • CORRECTIONS to Table 9-1 in your lab manual: 9. Two pairs of antennae; cephalothorax present........subphylum Crustacea/class Crustacea 9. One pair of antennae, cephalothorax absent................(mark out old subphylum here)...10 10. Wings usually present; three pairs of walking legs....subphylum Hexapoda/class Insecta 10. Wings absent; more than three pairs of walking legs..............subphylum Myriapoda...11 Task C-2: Invertebrate keying • fill in Table 9-3 for each organism in the box (TAs will be checking for this as part of your exit activity) • if you don’t know common names, ask a TA • not all have subphyla • you should expect to be able to make such phylum, subphylum, and class identifications on the lab final (without help or a key) for these and any other specimens that have been in lab Phylum Echinodermata • coelomate; unsegmented • pentamerous radial symmetry • water vascular system • dermal endoskeleton with spines Phylum Echinodermata • • 4 selected classes to examine in lab: • Asteroidea (sea stars or starfish) • Ophiuroidea (brittle stars) • Echinoidea (sea urchins and sand dollars) • Holothuroidea (sea cucumbers) watch the video for body plan and feeding…. Task A-1: Phylum Echinodermata • examine the preserved specimens, note the following: • for each: compare to Fig. 9-1, note body plan 5part radial symmetry, endoskeleton, lack of segmentation •Asteroidea (sea stars) – five arms, madreporite, central disc, mouth, tube feet, coelom •Ophiuroidea (brittle stars) – five arms, central disc Task A-1: Phylum Echinodermata • examine the preserved specimens, note the following: • Echinoidea (sea urchins and sand dollars) – look for pentamerous characteristics; jaws Task A-1: Phylum Echinodermata • examine the preserved specimens, note the following: • Holothuroidea (sea cucumbers) – not typically sedentary, just slow (lab manual is wrong) Radial muscle Cloaca & vent Phylum Chordata • group unified by four traits present at some point in the life cycle for all members Phylum Chordata • • three subphyla • Urochordata – tunicate or sea squirt • Cephalochordata – lancelet or amphioxus • Vertebrata – have a backbone; fish, amphibians, reptiles, birds, and mammals (classes listed later) watch the video for some information on the body plan and development…. Task B-1: Invertebrate Chordates • examine the preserved specimens and models, note the following: • Urochordata – tunicate or sea squirt • sessile adult; cellulose tunic, holdfast, siphons, pharynx • image on following slide…. Task B-1: Invertebrate Chordates • examine the preserved specimens and models, note the following: • Cephalochordata – lancelet or Amphioxus • specimen, model, and slide; match to Figs. 9-2 and 9-3 • note oral hood, fins, myotomes, dorsal nerve cord, notochord, pharynx, pharyngeal gill slits, postanal tail; other items from figures Subphylum Vertebrata • Vertebrata – 7 selected classes • Agnatha – jawless fish • Chondrichthyes – cartilaginous fish (sharks, skates, and rays) • Osteichthyes – bony fish with paired fins • Amphibia – two pairs of legs; smooth skin (frogs, salamanders, etc.) • Reptilia – two pairs of legs; scaly skin (lizards, snakes, alligators, etc.) • Aves – feathers; paired wings and legs (birds) • Mammalia – hair, mammary glands, two paired appendages Task B-2: Subphylum Vertebrata • obtain a “vertebrate box” and examine the preserved specimens, note the following: • Agnatha – brook lamprey • • jawless; single median dorsal and caudal fins (no paired lateral fins); 7 pairs of external gill slits Chondrichthyes – shark • scales (makes sandpapery skin); various fins (note especially those in pairs); claspers on pelvic fins of mature males; cloacal opening Task B-2: Subphylum Vertebrata • Osteichthyes – yellow perch • • scales, operculum over gills, various fins (note pairs); lateral lines (from operculum to caudal fin) Amphibia – leopard frog and salamander • smooth skin (no scales); 4 legs – count digits; tympanum on frog; eyelids (do other box items have eyelids?) Task B-2: Subphylum Vertebrata • Reptilia – green anole lizard, turtle, and garter snake • • • scaly skin, 4 legs (reduced to vestigial in snake); lizard – claws, eyelids; turtle – carapace and plastron Aves and Mammalia – no specimens demonstration table – identify specimens by class (you do NOT include them on Table 9-2, but they ARE eligible to be on the lab final)