MCB 3023 lab – tentative syllabus for Spring Week Topic Lab Manual Exercise 1, 3 Reading in Text 25,26 1 1/14 Introduction, Microscopy; Microbes in the environment 2 1/21 Wet mounts; Smears and simple staining 2, 5 26-28 3 1/28 Gram staining; acid fast staining Isolation of bacteria; carbohydrate metabolism fermentation, protein catabolism, respiration, UV radiation 7, 8, 11, 13 Special media; Biofilms – set up Test 1 Oxygen and bacterial growth; biofilms cont. 12, 21 26, 90-91, 403-405 373-378, 384-385, 146, 175, 270 86-90, 133 4 2/4 5 2/11 6 2/18 19 143-147 7 2/25 Growth curves; biofilms, set up Winogradsky Column 20, 56 Regulation of Gene expression; Transformation; Take home Sabouraud agar plate Plant viruses, Fungi Test 2 Start Koch’s postulates March 19th – last day to drop with a “W” Spring Break Phototrophs; Protozoa; Koch’s postulates 27, 29 123-126, 131-132, 644-646 212-217, 275-277 624, 601-607 8 3/4 9 10 3/11 3/18 11 3/25 4/1 12 4/8 Koch’s postulates, Microbes in Water, Bioremediation Microbes in soil – N and S cycles Microbes in water Finish Koch’s postulates and Winogradsky 52, 57 56 13 4/15 14 4/22 14, 15, 16, 17, 23 38; 33; 40 34, 35 53 591- 600; 607-610 15-18, 647648, 714-715 678-680, 703-706, 683-689, 1005 Test 3 Laboratory Manual: Laboratory Experiments in Microbiology by Johnson and Case, 10th ed. Lecture Text: Brock Biology of Microorganisms by Madigan, et al. 13th ed. Professor: Teresa G. Fischer Office Hours: posted online and on office door S208 Phone/E-Mail: 336-6261; [email protected] or via Angel LMS Biology department web page: biology-irsc.weebly.com Pre-Lab Quizzes: Before attending lab, students must have taken and scored a minimum of 70% on the Pre-Lab Quiz for that week’s exercises. Quizzes are taken online, and are found under the Lessons tab in the Angel LMS. The Pre-lab Quizzes are worth 10% of the semester grade Grades: Grades for the semester are based upon the average of the three exams given and the Pre-Lab Quizzes. See the attendance policy below. No extra credit will be offered. Grades are assigned on the following basis: A: 90-100, B: 80-89, C: 70-79, D: 60-69, F: below 60 Make-up policy: Students should take their lab practical with their lab section at the assigned time. If a student is unable to attend that practical, he/she MUST obtain permission within 24 hours in order to arrange to take the practical with his/her instructor in a different lab section during the test week (provided space is available), or discuss other options with his/her instructor. Attendance: Students are expected to attend all labs. Students are responsible for knowing exact dates and times of exams, results of lab experiments, exact arrangements for written assignments, etc. Students may attend a lab in another section if space is available. Be sure to give me the filled out make-up slip in order to get credit for attendance. One absence is allowed without penalty; any subsequent absence will result in a 10 % reduction in grade per absence. Students more than 10 minutes late will be considered absent, and must attend an alternate lab session. Appropriate Laboratory Attire: Closed toed and closed-heeled shoes are required. If long sleeves are worn, they should fit tight to the wrists and be free of ruffles. Students should dress in a conservative manner. Lab instructors have the right to refuse admittance to any students improperly dressed. Students are required to use lab coats in the Microbiology lab. Disposable coats to be used during one semester are available at the bookstore. The coats will be used and stored in the lab and autoclaved and discarded at the end of the semester. If you wish to supply your own cloth lab coat, you will not be allowed to take the lab coat home at the end of the semester. Withdrawal: Please contact the instructor if you feel that you need to withdraw. It might not be necessary. You will NOT be automatically withdrawn if you stop coming to class. Withdrawal requires that you submit an official IRSC form which can be obtained at Educational Services or any site office. Students wanting to withdraw must do so by the withdrawal date listed in the course schedule. Cheating: Cheating of ANY kind WILL NOT be tolerated by this Department. Anyone caught cheating will automatically receive an F for the semester. Anyone who witnesses this inexcusable behavior, and does not report it, is also considered to be guilty of improper conduct. Learning Outcome: Develop the knowledge skills, attitudes and values necessary for positions of responsibility in a variety of Life Science industries.