Which of the following bacteria is cell wall deficient? A. Mycoplasma. B. Treponema. C. Slaphylococcus. D. Klebsiella. E. Salmonella ANSWER: A What is unique about the Mycoplasma bacterial group? A. they lack a cell membrane B. they have organelles C. they are prokaryotes D. they have ribosome E. they lack cell walls ANSWER: E Mycoplasmas have not next structure. A. nucleus B. cell walls C. ribosomes D. mitochondria E. cell membranes ANSWER: B What microbes do belong to facultative anaerobes? A. Clostridium tetani, Clostridium perfringens B. Mycobacterium tuberculosis, Vibrio cholerae C. Corynebacterium diphtheriae, Salmonella typhi D. Brucella abortus E. Lactobacillus acidophilus ANSWER: C All of bacteria belong to obligate aerobes, EXCEPT: A. Mycobacterium tuberculosis B. Yersinia pestis C. Micrococci spp. D. Clostridium tetani E. Vibrio cholerae ANSWER: D Which of the following is not an appropriate specimen to culture for the presence of pathogenic anaerobes? A. sinus aspirate B. bone C. blood D. bile E. sputum ANSWER: E One common cause of community-acquired pneumonia is: A. Salmonella typhimurium B. Escherichia coli C. Enterobacter D. Shigella flexneri E. Klebsiella pneumoniae ANSWER: E Which of the following bacteria belong/s to the Enterobacteriaceae family? A. Shigella. B. Salmonella. C. Yersinia. D. Proteus E. All answers are corect ANSWER: E What is the most common cause of urinary tract infections? A. Staphylococcus saprophyticus B. Escherichia coli C. Enterococcus fecalis D. Streptococcus bovis E. Staphylococcus epidermidis ANSWER: B A sexually-active woman develops a urinary tract infection which ascends to the kidneys. Blood culture yields a beta-hemolytic Gram-negative rod. In this situation, which pathogen is most common? A. Klebsiella pneumoniae. B. Pseudomonas aeruginosa. C. Salmonella enteritidis. D. Serratia marcescens. E. Escherichia coli. ANSWER: E Humans can get shigellosis from: A. dogs B. cats C. swine D. chickens E. humans ANSWER: E Toxin of Vibrio cholerae is: A. LPS B. Toxic shock syndrome toxin C. Enterotoxins D. Hemolysins E. Toxin - activates adenylate cyclase ANSWER: E Toxicogenity of Vibrio cholerae is accociated with: A. LPS B. Toxic shock syndrome toxin C. Toxin - activates adenylate cyclase D. Enterotoxins E. Hemolysins ANSWER: C Which of the following microorganims can cause severe dehydration? A. Lactobacilli. B. Virbrio cholerae C. Staphylococcus aureus D. Clostridium tetani E. Neisseria gonorrheae ANSWER: B The best treatment for cholera is A. antiserum injection B. tetracycline C. toxoid D. vaccine E. rehydration therapy ANSWER: E Which of the following bacteria can grow in alkaline pH? A. Lactobacilli. B. Vibrio cholerae. C. Salmonella. D. Shigella. E. Neisseria ANSWER: B Shigella is transmitted by A. infected food and water B. ticks C. direct contact D. air dropled E. louse ANSWER: A What disease can cause Salmonella typhi? A. Lymphogranuloma venereum B. Sepsis C. Necrotising faciitis D. Enteric fever E. Subacute sclerosing panencephalitis ANSWER: D In enteric fever, Salmonella typhi may be isolated from: A. urine. B. faeces. C. blood. D. bile E. all answers are true. ANSWER: E Which of the following bacteria is/are primarily human pathogens? A. Salmonella typhi. B. S. paratyphi A. C. S. paratyphi B. D. Shigella boydi E. All answers are true. ANSWER: E A stool sample is plated on standard Endo-agar plates. After overnight incubation some of the colonies are dark pink in color, others are pale, nearly colorless. What can you conclude about the bacteria that produced dark-pink colonies? A. Gram-positive B. Not likely to be ‘normal flora’. C. Non-motile. D. Produce capsules. E. Ferment lactose. ANSWER: E Which bacteria doform dark pink colonies on Endo medium? A. Salmonella enterica B. Escherichia coli C. Listeria monocytogenes D. Shigella sonnei E. Shigella dysenteriae ANSWER: B On bacteriological examination of vomiting mass of the patient with food poisoning, the pure bacterial culture was isolated with the following properties: gram-negative motile bacillus in the Endo medium grows like colourless colony. Representative of what genus has caused the illness? A. Salmonella B. Shigella C. Yersinia D. Escherichia E. Citrobacter ANSWER: A Which of the following specimens is/are most appropriate for culture of bacteria in first week of enteric fever? A. Blood. B. Urine. C. Faeces. D. CSF. E. Bile ANSWER: A A child has gotten bloody diarrhea, caused by Shigella. What from following is/are source of this infection? A. Hamburger. B. Dog or cat. C. Cow, horse, or sheep. D. Another sick child. E. Spores present in soil. ANSWER: D Bipolar staining is characteristic of: A. Yersinia pestis. B. Staphylococcu aureus C. Clostridium botulinum D. Vibrio cholerae E. all answers are true ANSWER: A Pneumonic plague can be transmitted from man to man by: A. droplet infection. B. inoculation. C. ingestion. D. flea bite E. all modes. ANSWER: A The name 'black death' is given to which of the following diseases? A. Tuberculosis. B. Diphtheria. C. Plague. D. AIDS. E. Cholera ANSWER: C Which one of the following zoonotic illnesses has NO arthropod vector? A. Plague B. Lyme disease C. Brucellosis D. Epidemic typhus E. Anthrax ANSWER: C This bacterium, although usually not considered as normal flora, frequently colonizes the stomach and is thought to be the cause of gastric and duodenal ulcers. A. Lactobacillus acidophilus B. Pseudomonas aeruginosa C. Escherichia coli D. Vibrio cholerae E. Helicobacter pylori ANSWER: E A patient has a peptic ulcer. Which organism is most likely to have created this lesion? A. Campylobacter jejuni. B. Enterococcus faecalis. C. Escherichia coli. D. Helicobacter pylori E. Salmonella enteritidis. ANSWER: D A patient has chronic gastritis. Which of the following organisms is most likely? A. Campylobacter fetus. B. Campylobacter jejuni. C. Helicobacter pylori. D. Escherichia coli. E. A non-cholera Vibrio. ANSWER: C Which of the following bacterium is causative agent of dysenteriae? A. E. coli B. Klebsiella C. Proteus D. Vibrio E. Shigella ANSWER: E A unique characteristic of many isolates of Pseudomonas useful in identification is A. drug resistance B. fecal odor C. green pigment D. gram staining E. motility ANSWER: C Urine examination of a patient with acute cystitis revealed leukocytes and a lot of gram-negative bacilli. Inoculation resulted in growth of colonies of mucous nature that formed green soluble pigment. What microorganism is the most probable cause of the disease? A. Pseudomonas aeruginosa B. Escherihia coli C. Klebsiella pneumoniae D. Proteus mirabilis E. Salmonella enteritidis ANSWER: A A classic symptom of pertussis is A. diarrhea B. malaise C. headache D. paroxysmal coughing E. skin rash ANSWER: D A child is diagnosed with Pertussis. How was this infection most likely to have been acquired? A. Bite of a dog or cat. B. Consumption of dairy products made from unpasteurized milk C. Contact with livestock or animal products such as leather or wool. D. Inhalation of respiratory droplets of another human being. E. Release of oral flora into the bloodstream by dental work ANSWER: D What diseases can be transferred through water more often? A. Tuberculosis, tetanus B. Typhoid fever, dysentery, hepatites A C. Anthrax, plagues, tularemia D. Flu, measles, diphtherias E. Botulism, gas gangren infections, tetanus ANSWER: B Specify disease which basis of treatment is application of an antitoxin A. typhoid B. nocardiosis C. eryzopeloid D. diphtheria E. syphilis ANSWER: D A man died from an acute infectious disease accompanied by fever, jaundice, haemorrhagic rash on the skin and mucous membranes as well as by acute renal insufficiency. Histological examination of renal tissue (stained by Romanovsky-Giemsa method) revealed some convoluted bacteria looking like C und S letters. What bacteria were revealed? A. Leptospira B.Treponema C.Spirilla D.Borrelia E. Campilobacteria ANSWER: A While studying a microslide obtained from the punctuate of a regional lymph node and stained by Romanovsky-Giemsa method a physician revealed some light-pink thin microorganisms with 12-14 regular spiral coils and pointed ends, up to 10-13 micrometer long. This might be the causative agent of the following disease: A. Syphilis B. Trypanosomiasis C. Leptospirosis D. Relapsing fever E. Leishmaniasis ANSWER:A What is morphology of Treponema pallidum? A. Spiral-shaped B. Mold C. Vibrio D. Rod E. Yeast ANSWER: A Syphilis is caused by which of the following microorganisms? A. Helicobacter pylori B. Streptococcus pyogenes C. Treponema pallidum D. Staphylococcus aureus E. Clostridia tetanus ANSWER: C The symptom of secondary syphilis is A. neurological problems B. giant lesions affecting many internal organs C. a hard-based, painless chancre D. a rash covering all parts of the body E. Meningitis ANSWER: D Which of the following groups of people is most at risk for contracting T. pallidum? A. Pregnant mothers B. Cystic fibrosis patients C. Sexually active patients D. Elderly patients E. Anyone (spread through contact with inanimate objects, such as toilet seats) ANSWER: C While studying a microslide obtained from the punctuate of a regional lymph node and stained by Romanovsky-Giemsae method a physician revealed some light-pink thin microorganisms with 12-14 regular spiral coils and pointed ends, up to 10-13 micrometer long. This might be the causative agent of the following disease: A. Syphilis B. Trypanosomiasis C. Leptospirosis D. Relapsing fever E. Leishmaniasis ANSWER: A Indicate microbe which has sexual mode of transmission: A. Treponema pallidum B. Borrelia recurrentis C. Borrelia burgdorferi D. Leptospiras interrogans E. Treponema vincentii ANSWER: A Relapses in relapsing fever occur due to: A. antigen variation in bacteria. B. increased toxin production by bacteria. C. increased invasiveness of bacteria. D. super infection with viruses. E. increased fermentative activity of bacteria ANSWER: A Indicate disease caused by Borrelia burgdorferi: A. Weil syndrome (icterohemorrhagic fever), canicola fever B. Endemic relapsing fever C. Lyme disease D. Syphilis E. Enteric fever ANSWER: C Lyme disease is caused by: A. Borrelia recurrentis. B. B. dutioni. C. B. burgdorferi. D. B. hermsii E. B. persica ANSWER: C What disease is caused by Borellia burgdorferi? A. Lyme disease B. Rocky Mountain Spotted Fever C. Hanta D. Rabies E. Relapsing fever ANSWER: A Causative agent of tuberculosis is: A. Staphylococus aureus B. Clostridium tetani. C. Bacilus subtilis D. Mycobacterium tuberculosis E. Salmonella typhi ANSWER: D A consumptive patient has an open pulmonary form of disease. Choose what sputum staining should be selected for finding out the tubercule (Koch's) bacillus? A. Ziel-Neelsen method B. Romanowsky-Giemsa method C. Gram method D. Neisser method E. Burry-Gins method ANSWER: A Microscopy of stained (Ziehl-Neelsen staining) smears taken from the sputum of a patient with chronic pulmonary disease revealed red bacilli. What property of tuberculous bacillus was shown up? A. Acid resistance B. Alkali resistance C. Alcohol resistance D. Capsule formation E. Sporification ANSWER: A The first grade pupils were examined in order to sort out children for tuberculosis revaccination. What test was applied for this purpose? A. Mantoux test B. Schick test C. Supracutaneous tularin test D. Burnet test E. Anthraxine test ANSWER: A Planned mass vaccination of all newborn 5-7 day old children against tuberulosis plays an important role in tuberculosis prevention. In this case the following vaccine is applied: A. BCG B. Diphteria and tetanus toxoids and pertussis vaccine C. Diphtheria and tetanus anatoxin vaccine D. Adsorbed diphtheria vaccine E. Sabin vaccine ANSWER: A BCG vaccine is a: A. live attenuated preparation. B. killed preparation C. toxoid preparation D. recombinant preparation. E. vector vaccine ANSWER: A Which of the following vaccines are prepared from live microbes? A. diphtheria vaccine B. Solk polio vaccine. C. Influenza vaccine D. DPT E. BCG and oral polio vaccines ANSWER: E BCG vaccine is used for prevent: A. tuberculosis B. diphtheria C. botulism D. brucelosis E. Q fever ANSWER: A In the upper respiratory tract of patient doctor observed thick grey ‘pseudomembrane’ formation. Which organism can cause this symptoms? A. Mycobacterium tuberculosis B. Legionella pneumophilia. C. Streptococcus mitis D. Corynebacterium diphtheriae E. Pseudomonas aeruginosa ANSWER: D The main carrier site on the human body for strains of potentially pathogenic Staphylococcus aureus is the A. oral cavity B. throat (posterior nasopharynx) C. nasal membranes D. gastrointestinal tract E. vagina ANSWER: B Scalded skin syndrome is due to which toxin of Staphylococcus aureus? A. Epidermolytic toxin. B. Enterotoxin. C. Leucocidin. D. Haemolysin E. Necrotoxin ANSWER: A Which of the following bacteria ferment mannitol anaerobically? A. Staphylococcus aureus. B. S. epidermidis. C. S. saprophyticus. D. S. canis E.None of them. ANSWER: A The coagulase test is used to differentiate Staphylococcus aureus from A. other staphylococci B. streptococci C. micrococci D. enterococci E. pneumococci ANSWER: A The coagulase test, in which the bacteria cause plasma to clot, is used to distinguish A. Streptococcus pyogenes from Enterococcus faecalis B. Streptococcus pyogenes from Staphylococcus aureus C. Staphylococcus aureus from Staphylococcus epidermidis D. E. coli from Neisseria meningitidis E. Clostridium perfringens from Borrelia recurrentis ANSWER: C The patient has pustular skin lesions. Isolated from patient’s sample a causative agent forms in the blood agar yellow middle-sized colonies surrounded by haemolysis zone. Smears from the colonies contain irregular-shaped clusters of gram-positive cocci. The culture ferments mannitol and synthesizes plasmocoagulase. What causative agent was isolated? A. Staphylococcus aureus B. Streptococcus agalactiae C. Streptococcus pyogenes D. Staphylococcus epidermidis E. Staphylococcus saprophyticus ANSWER: A Phenotypic characteristic of Staphylococcus aureus is: A. Enveloped B. Motility "+" C. ssRNAD. Oxidase "-" E. Coagulase "+" ANSWER: E Analysis of sputum taken from a patient with suspected pneumonia revealed gram-positive capsuleted diplococci. What microorganisms were revealed in the sputum? A. Streptococcus pneumoniae B. Staphylococcus aureus C. Klebsiella pneumoniae D. Neisseria meningitidis E. Neisseria gonorrhoeae ANSWER: A A child develops pneumonia. The bacterium Streptococcus pneumoniae is cultured from the patient’s sputum. Cells of the cultured bacteria have thick polysaccharide capsules. How did the presence of capsules promote development of serious disease? A. Prevented binding of antibodies to the bacteria. B. Prevented initiation of a humoral immune response C. Prevented activation of helper T cells D. Prevented mast-cell degranulation. E. Prevented phagocytosis by neutrophils. ANSWER: E A patient presents to the emergency room after several days of 'flu-like' symptoms. You collect a sputum sample on which the laboratory reports Gram positive lancet shaped diplococci. What is your diagnosis? A. S. pneumoniae pneumonia B. Clostridium difficile C. Staphylococcus spp. D. Viridans streptococci E. E. coli Morphology of Pneumococcus is: A. Rod B. Cocci in chains C. Cyst D. Spiral-shaped E. Diplococci ANSWER: E Which one of the following is not an important characteristic of Streptococcus pneumoniae? A. Alpha- hemolytic B. Causes otitis media C. Polysaccharide capsule D. Normal intestinal flora E. Gram-positive lancet-shaped diplococci ANSWER: D In what way do Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae most closely resemble each other? A. Polysaccharide capsules B. Lipopolysaccharide in their outer membranes. C. Teichoic acid in their envelopes. D. Thick peptidoglycan in their cell walls. E. Motility due to flagella. ANSWER: A Of the organisms listed below, which one is the MOST frequent bacterial cause of pharyngitis? A. Staphylococcus aureus B. Streptococcus pneumoniae C. Streptococcus pyogenes D. Neisseria meningitidis E. Escherichia coli ANSWER: C The clinical diagnosis of a female patient is acute gonorrhoea. What method of examination can be applied for confirmation of this diagnosis? A. Microscopy of pathological material B. Infection of laboratory animals C. Test with bacteriophage D. Hemagglutination reaction E. Immobilization reaction ANSWER: A Microorganisms capable of producing endospores include: A. Escherichia species B. Bacterium species C. Leptospirae D. Staphylococci E. Clostridium species ANSWER: E A young man complaines on pain on urination and profuse purulent discharge from his urethral opening. Gram stain of the discharge revealed large numbers Gram-negative cocci, mostly in pairs within neutrophils. Which bacterium is most likely to have caused his infection? A. Mycoplasma hominis B. Chlamydia trachomatis C. Neisseria gonorrhoeae D. Ureaplasma urealyticum E. Treponema pallidum ANSWER: C What from the listed microorganisms can exist long time in soil? A. Causetive agents of typhoid fever and dysentery B. Causetive agents of tuberculosis and diphtheria C. Hepatites A and B viruses D. Hemophylus influenzea and streptococus pneumonias E. Causetive agents of anthrax and botulism ANSWER: E What is the usual habitat of endospore-forming bacteria that are agents of disease? A. fomites B. soil C. water D. foods E. dust ANSWER: B It is planned to use the territory of an old cattle burial ground (which is not used for more than 50 years) for building houses. But ground analysis revealed presence of the pathogen of the very dangerous illness. Which of the indicated microorganisms is likely to remain in the ground for such a long time? A. Bacillus anthracis B. Francisella tularensis C. Brucella abortus D. Yersinia pestis E. Mycobacterium bovis ANSWER: A Typical drumstick appearance of bacilli is observed in: A. Clostridium perfringens. B. C. tetani. C. С. botulinum. D. C. histolyticum. E. C. difficilae ANSWER: C The causative agent/s of gas gangrene is/are: A. Clostridium perfringens. B. C. novyi. C. С. septicum. D. C. histolyticum E. all of listed microbes. ANSWER: E Resident flora can be found in all of the following locations in the human body EXCEPT the: A. eye B. skin C. blood D. mouth E. large intestine ANSWER: C Which of the following exotoxins is most toxic? A. Botulinum toxin. B. Tetanus toxin. C. Diphtheria toxin. D. Cholera toxin. E. Shigella toxin ANSWER: A Bacterial genus/genera of medical importance which produce endospores is/are: A. Bacillus. B. Bacterium. C. Corynebacterium D. Mycobacterium E. Micrococcus ANSWER: A Indicate among these microbes obligate anaerobic bacteria: A. Staphylococcus spp., Streptococcus spp. B. Clostridium perfringens, Bacteroides spp. C. Escherichia coli, Salmonella typhi D. Bacillus anthracis, Brucella abortus E. Mycobacterium tuberculosis, Corynebacterium diphtheriae ANSWER: B An infant is brought to the emergency room, cyanotic and with poor muscle tone. Which condition is most likely given these findings? A. Antibiotic-associated colitis. B. Diphtheria. C. Anthrax. D. Botulism. E. Tetanus. ANSWER: D Which of the following microorganisms do produce the tetanospasmin? A. M. tuberculosis B. C. tetani C. C. diphtheriae D. S. aureus E. E. coli ANSWER: B In Europe human Tetanus is now a rare disease. Which measure below has been most important in preventing it? A. Vaccination of livestock, especially cattle, with a killed vaccine B. Immunization of humans with a toxoid-containing vaccine. C. Eradication of the organism from the environment. D. Sewage treatment and purification of water supplies. E. Antibiotic treatment of contacts of patients with C. tetani infections. ANSWER: B Which of the following is associated with Floppy Baby Syndrome? A. C. difficile B. C. perfingens C. C. septicum D. C. botulinum E. C. tetani ANSWER: D In which of the following anatomical locations would you expect to find the largest microbial population? A. mouth B. skin C. kidneys D. large intestine E. upper respiratory tract ANSWER: D Bubonic plague is transmitted by: A. rat flea. B. inhalation. C. ingestion. D. lice E. water ANSWER: A Examination of a child revealed some whitish spots looking like coagulated milk on the mucous membrane of his cheeks and tongue. Analysis of smears revealed gram-positive oval yeast-like cells. What causative agents are they? A. Candida B. Staphylococci C. Diphtheria bacillus D. Actinomycetes E. Fusobacteria ANSWER: A As a result of long duration of antibiotic therapy a 37-year old patient has gotten intestinal dysbacteriosis. What type of medicines should be used to normalize intestinal microflora in order? A. Eubiotics B. Sulfanilamides C. Bacteriophages D. Autovaccines E. Vitamins ANSWER: A Bacteriological examination of purulent discharges from the urethra revealed some gram-negative beanshaped bacteria located in the leukocytes. They can be identified as the causative agent of the following disease: A. Gonorrhea B. Syphilis C. Venereal lymphogranulomatosis D. Chancroid E. Trichomoniasis ANSWER: A Neisseria gonorrhoeae are A. Fungi B. Helminths C. Bacteria D. Viruses E. Protozoa ANSWER: C Which genus of bacteria has pathogens that can cause blindness in newborn? A. Branhamella B. Staphylococcus C. Streptococcus D. Shigella E. Neisseria ANSWER: E Smear of CSF sediment from a patient with meningitis stained by Gram method is contained paired Gramnegative cocci, many of them are inside of leucocytes. Which microorganism below is most likely? A. Streptococcus pneumoniae B. Haemophilus influenzae C. Pseudomonas aeruginosa D. Escherichia coli E. Neisseria meningitidis ANSWER: E Morbidity of Candida albicans is A. Endocarditis B. Pneumonia C. Bronchopneumonia D. Bladder carcinoma E. Systemic candidiasis ANSWER: E Choose among listed microorganisms which occupy a soil: A. Mycobacterium tuberculosis, Corynebacterium diphtheriae, Staphylococcus B. Nitrosomonas, Azotobacter, Actynomycetes C. Bacteroides, Vibrio, Spirillum D. Brucella, Bacillus anthracis, Clostridium E. Salmonella, Shigella, Leptospira ANSWER: B Match the organism with its usual colonizing site - Coagulase negative Staphylococcus A. Intestine B. Vagina C. Mouth D. Conjuctiva E. Skin ANSWER: E Match the organism with its usual colonizing site -Lactobacillus spp A. Blood B. Skin C. Mouth D. Conjuctiva E. Vagina ANSWER: E Match the organism with its usual colonizing site -Enterococcus faecalis A. Skin B. Vagina C. Mouth D. Conjuctiva E. Intestine ANSWER: E Streptococcus pneumoniae can be part of the normal flora of 5-40 % of people. At what anatomic site can it be found7 A. Conjunctiva B. Colon C. Urethra D. Vagina E. Nasopharynx ANSWER: E Ascoly thermoprecipitin test helps in confirming the laboratory diagnosis of: A. tetanus. B. typhoid. C. cholera. D. pertussis E. anthrax. ANSWER: E Rickettsias and chlamydias are similar in being A. carried by arthropod vectors B. free of a cell wall C. produce exotoxin D. the cause of eye infections E. obligate intracellular bacteria ANSWER: E You suspect Chlamydia infection in a sexually-active young woman. Which test would be most likely to provide results which would confirm this diagnosis? A. Agglutination test for anti-cardiolipin antibodies. B. Test of a blood sample for ‘cold agglutinins”. C. Gram-stained smear of discharge from the cervix. D. Culture of exudate from the cervix on Thayer-Martin agar. E. PCR test of nucleic acids present in a urine sample. ANSWER: E Which one of the following zoonotic illnesses has NO arthropod vector? A. Plague B. Lyme disease C. Brucellosis D. Epidemic typhus E. Anthrax ANSWER: C