Online labs ch - ontario high school library

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Online labs ch.13
Name:
Directions: Go to the school’s library home page. Click on Miss Sweet’s Gene labs. Click on the link’s that
follow. Answer the questions as you go through each interactive.
DNA Extraction lab: http://learn.genetics.utah.edu/content/labs/extraction/
1. Why do scientists isolate DNA?
-_________________________
-_________________________
-_________________________
2. Why is DNA extraction important?
3. How long is the DNA in one nucleus?
4. What are the 4 steps of purifying DNA?
1.
2.
3.
4.
5. What does the word ‘lysis’ mean?
6. What is the purpose of the detergent in the lysis solution?
7. What is the purpose of the salt?
8. What is the purpose of the centrifuge?
9. What is the purpose of the isopropyl alcohol?
DNA fingerprint lab: http://www.teachersdomain.org/asset/tdc02_int_creatednafp2/
10. What happened to Jimmy Sweet?
11. What are the steps to creating a DNA fingerprint, List the steps here:
Step 1:
Step 2:
Step 3:
Step 4:
Step 5:
Step 6:
Step 7:
Step 8:
Step 9:
-What is the purpose of restriction enzymes?
-Define electrophoresis.
-When finished, how will the fragments be distributed?
-Who was the culprit? How do you know?
Crime scene/Paternity lab:
http://www.biotechnologyonline.gov.au/biotechnologyonline/popups/int_dnaprofiling.html
Robbery investigation:
12. Summarize the crime13. Which blood sample is a match?
14. Who does the hair belong to?
15. Does the DNA sample place the suspect at the scene of the crime? Explain.
Investigate the Will:
16. Is Percy the father of all 3 children? How do you know?
17. Is Harriet the mother of all 3 children? How do you know?
18. Who is not Harriet’s child?
19. Does Alistair have right’s to Percy’s fortune?
Cloning Lab: http://learn.genetics.utah.edu/content/tech/cloning/clickandclone/
20. What does somatic cell nuclear transfer mean?
21. What are the steps to cloning Mimi:
Step 1.
Step 2.
Step 3.
Step 4.
Step 5.
Step 6.
-What color will the clone be? Why?
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