Lab One: Is the Red Stain Actually Blood
Blood And Serology
Forensics
MissL
2015-6
Chapter 8 Blood and Blood Spatter
Introduction
History of the Study of Blood
Composition of Blood
Blood Cells
Blood Types and Forensics
Antigen-Antibody Response
Probability and Blood Types
Blood-Spatter Patterns
History of Blood-Spatter Analysis
Blood-Spatter Pattern Analysis
Directionality of Blood
Bloodstain Patterns
Area of Convergence
Angle of Impact Calculations
Blood Velocity and Spatter Size
Crime-Scene Investigation of Blood
Confirmation of Blood
Collection of Blood Evidence
Chapter Summary
Case Studies
Careers in Forensics Bloodstain Pattern Analyst
Chapter 8 Review
Activity 8-1 A Presumptive Test for Blood
Activity 8-2 Creating and Modeling Blood-Spatter
Patterns
Activity 8-3 Blood-Spatter Analysis: Effect of Height
on Blood Drops
Activity 8-4 Area of Convergence
Activity 8-5 Blood-Drop Angle Impact
Activity 8-6 Area of Origin
Activity 8-7 Crime-Scene Investigation
STEM Topics
Next Generation Science Standards (NGSS)
HS-ETS1-2 Design a solution to a complex real-world
problem by breaking it down into smaller, more
manageable problems that can be solved through
engineering.
HS-PS1-2 Construct and revise explanation for the outcome of a simple
chemical reaction based on the outermost electron states of atoms,
trends in the periodic table and knowledge of the patterns of chemical
properties.
HS-LS3-3 Apply concepts of statistics and probability to explain the
variation distribution of expressed traits in a population.
mod
names
roles
Common Core (CCSS Literacy)
RST.9-10.3 Follow precisely a multistep procedure when
carrying out experiments.
RST.9-10.4 Determine the meaning of symbols, key terms and
phrases.
RST.11-12.1 Cite specific textual evidence to support analysis
of science.
RST.11-12.3 Follow precisely a multistep procedure when
carrying out experiments.
RST.11-12.4 Determine the meaning of symbols, key terms and
phrases.
WHST.9-10.1 Write arguments focused on science content.
WHST.9-10.2 Write informative/explanatory texts, including
the narration of historical events, scientific
procedures/experiments or technical processes.
WHST.11-12.2 Write informative/explanatory texts, including
the narration of historical events, scientific
procedures/experiments or technical processes.
Common Core (CCSS Mathematics)
HSN.Q.A.1 Use units as a way to understand problems and to
guide the solution of multi-step problems; choose and
interpret units consistently in formulas; choose and interpret
the scale and the origin in graphs and data displays.
HSN.VM.A.1 Recognize vector quantities as having both
magnitude and direction. Represent vector quantities by
directed line segments, and use appropriate symbols for
vectors and their magnitudes.
HSG.SRT.C.7 Explain and use the relationship between the sine
and the cosine of complementary angles.
Focus: when you see a red stain at a crime scene, is it really blood?
Pre Lab- Questions:
1. the Kastle Meyer Test: why does it turn pink in the presence of blood?
2. What in blood causes the luminal test to glow?
3. Why does H2O2 bubble in the presence of living tissue?
Step One: let’s go here and see the color changes we are looking for.
4. Hydrogen peroxide test for blood https://www.youtube.com/watch?v=aCQIxpbss94
The reaction of hydrogen peroxide 30% and blood
5. https://www.youtube.com/watch?v=sF0Z0jJGwX8 Kastle-Meyer Test 1.02
6. https://www.youtube.com/watch?v=u73H-5-RP5Y Detecting blood using luminol
Data table One: Is the Stain Actually Blood
Item ##
Catalase reaction:
bubbling with
H2O2
** Blood in spot
plate, add H2O2
Most obvious test
for living
material.
Did it bubble? Is
it living?
Reaction with Kastle
Meyer/Phenolthalian
Reaction with
Luminol
More presumptive test
for blood.
Fairly certain test
for blood.
Blood, alcohol, KM,
H2O2, in that order, on
spot plate
Alcohol, blood
Luminol, H2O2, in
that order, in the
sport plate
Did it turn pink?
Could it be blood?
Is It Really Blood?
Describe why you
can very likely say
it really is blood??
Did it light up blue?
Could it be blood?
Item 1
Item 2 etc
Add lines!!
Look at the prep
bench for the
number of things
http://www.texasobserver.org/a-bloody-injustice/
Bloody Injustice
1. As the dispatcher contacts paramedics, Warren can be heard in the background yelling, “Why’d you
do that, goddammit. Why? Why? Why? Why?” When he returns to the phone, he is panicking. “Are you
there? My wife….
2. By the time paramedics and police arrived, Bonnie Horinek had died. She was lying on the bed in her
pink nightgown with a single……..
3. There were two possible scenarios: Either Bonnie Horinek had committed…………..
4. Horinek’s fate would hinge on a few specks of blood found at the scene. A few specks of blood, that
is, along……..
5. Bonnie’s parents hired an attorney and a private investigator, who unearthed…….
6. THIS CASE HAS HAUNTED ME since 1995,” says Jim Varnon. “There are dozens of reasons that all
indicate this was a suicide.” He always believed Warren Horinek was innocent. And for the past 13
years….
7. He and Bonnie returned home from TGI Friday’s and got ready for bed. Warren went to his study to
check messages (he had a home business that provided companies with language translators). He heard
a single gunshot. He assumed someone……….
8. Zannin and MacDonell contend—in a report and two affidavits—that blood spots smaller than one
millimeter aren’t necessarily the result of a gunshot, as Bevel testified. Flecks that small can often…
9. To prove it, MacDonell set up an experiment at his lab in Corning, N.Y., in which a student placed a
small amount of blood in his mouth and then simply breathed on a white shirt. The result was…