Kent SeaTech Corporation IFAFS Proposal Kent SeaTech Water Sharing IFAFS Proposal Consortium Members: 1) Kent SeaTech Corporation, San Diego, California (Lead Institution) Principal Investigators: Mr. James M. Carlberg and Mr. Jon C. Van Olst Principal Investigators: Mr. Michael J. Massingill and Mr. Rodney J. Chamberlain 2) University of Arizona, Tucson, Arizona Principal Investigator: Dr. Kevin Fitzsimmons, Dept. of Soil, Water, and Env. Science Principal Investigator: Dr. Jeffrey C. Silvertooth, Plant Sciences Department 3) Clemson University, Clemson, South Carolina Principal Investigator: Dr. Dave E. Brune, Dept. of Agricultural & Biological Engineering 4) University of California Cooperative Extension Service Principal Investigator: Dr. Fred Conte, Aquaculture Extension Specialist, UC Davis Principal Investigator: Mr. Jose L. Aguiar, Farm Advisor, UC Riverside 5) McMullen Valley Water Conservation and Drainage District, Vicksburg, AZ Principal Investigator: Mr. James D. Downing, P.E. 6) Vicksburg Farms, Vicksburg, Arizona Principal Investigator: Mr. R. O. Cramer, General Partner 7) USDA Western Regional Aquaculture Center, Seattle, WA Principal Investigator: Dr. Kenneth Chew, Director This is a preliminary draft of the IFAFS water sharing proposal. It includes the overall concepts that we are hoping to address, but still is lacking an APPROACH section (other than a general task outline). We could use your help in adding to this outline and in adding a paragraph about the portion of the work that you will be responsible for. We also would appreciate any and all editorial suggestions you would like to provide. You can either make comments in a different color in this file and return it to jvanolst@west.net, or print the file, mark it up, and fax your suggestions to 805-649-9081. Thanks very much, Jack Van Olst Director of Research Kent SeaTech Corp Kent SeaTech Corporation IFAFS Proposal U.S. DEPARTMENT OF AGRICULTURE Cooperative State Research, Education, and Extension Service Initiative for Future Agriculture and Food Systems (IFAFS) JAWS: Joint Aquaculture/Agriculture Water Sharing Programs for Manure Management TABLE OF CONTENTS Project Summary.................................................................................................. (not numbered) Project Description...................................................................................................................... 1 A. Introduction ....................................................................................................................... 1 B. Relevance and Significance ............................................................................................... 1 C. Approach ........................................................................................................................... 1 D. Time Table......................................................................................................................... 1 E. Evaluation and Monitoring ................................................................................................ 1 1) Evaluation and Monitoring of Project Results .......................................................... 1 2) Evaluation and Monitoring of Consortium Administration ...................................... 1 F. Collaborative Arrangements............................................................................................... 1 G. Need for Consortium Approach ........................................................................................ 1 H. Consortium Management Plan .......................................................................................... 1 Appendices to Project Description.............................................................................................. 1 Key Personnel ............................................................................................................................. 1 Conflict-of-Interest List ............................................................................................................. 1 Collaborative and/or Subcontractual Arrangements ................................................................... 1 Budget (Form CSREES-55) ....................................................................................................... 1 Current and Pending Support (Form CSREES-663) ................................................................. 1 Kent SeaTech Corporation IFAFS Proposal Assurance Statements (Form CSREES-662) ............................................................................. 1 Certifications ............................................................................................................................... 1 Compliance with NEPA (Form CSREES-1234) ....................................................................... 1 Kent SeaTech Corporation IFAFS Proposal USDA Cooperative State Research, Education, and Extension Service Initiative for Future Agriculture and Food Systems (IFAFS) JAWS: Joint Aquaculture/Agriculture Water Sharing Programs for Manure Management PROJECT SUMMARY (250 Words) The aquaculture industry needs water to expand, but most suitable supplies are already being utilized by land-based agriculture. Our USDA and NIST research indicates that a water sharing approach may allow high density aquaculture operations to be located adjacent to agricultural operations, utilize the source water in a non-consumptive manner, treat it at minimal cost, and deliver it to row crop farms. Fish manure that represents a disposal problem to aquaculturists becomes an asset to downstream agriculture operations, providing nitrogen fertilizer for row crops via fertigation. Multiple-uses of limited water resources allows the two industries to share a single water source, reduce environmental pollution, and effectively double crop production. We will develop aquaculture/agriculture water sharing technologies and conduct education and extension activities to implement this technology in the western states, where 89% of irrigated crops are located. The research will take place in California and at a new aquaculture/agriculture research facility in Arizona that will be designed to develop and showcase the most efficient water sharing technologies available. Founded in 1972, Kent SeaTech has conducted 15 aquaculture research projects for USDA, NSF, DOC, and NIST. We will join with university scientists and extension experts to develop costefficient methods of interfacing aquaculture and agriculture facilities so that existing supplies of valuable irrigation water can be shared by both groups to conserve resources, reduce environmental pollution, and increase profitability. If this technology proves successful and were practiced at 4% of U.S. farms, aquaculture production could double, with no additional water resources required. CONSORTIUM MEMBERS 1) Kent SeaTech Corporation, San Diego, California (Lead Institution) Principal Investigators: Mr. James M. Carlberg and Mr. Jon C. Van Olst Principal Investigators: Mr. Michael J. Massingill and Mr. Rodney J. Chamberlain 2) University of Arizona, Tucson, Arizona Principal Investigator: Dr. Kevin Fitzsimmons, Dept. of Soil, Water, and Env. Science Principal Investigator: Dr. Jeffrey C. Silvertooth, Plant Sciences Department 3) Clemson University, Clemson, South Carolina Principal Investigator: Dr. Dave E. Brune, Dept. of Agricultural & Biological Engineering 4) University of California Cooperative Extension Service Principal Investigator: Dr. Fred Conte, Aquaculture Extension Specialist, UC Davis Principal Investigator: Mr. Jose L. Aguiar, Farm Advisor, UC Riverside 5) McMullen Valley Water Conservation and Drainage District, Vicksburg, AZ Principal Investigator: Mr. James D. Downing, P.E. 6) Vicksburg Farms, Vicksburg, Arizona Principal Investigator: Mr. R. O. Cramer, General Partner Kent SeaTech Corporation IFAFS Proposal 7) USDA Western Regional Aquaculture Center, Seattle, WA Principal Investigator: Dr. Kenneth Chew, Director Kent SeaTech Corporation IFAFS Proposal Page 1 USDA Cooperative State Research, Education, and Extension Service Initiative for Future Agriculture and Food Systems (IFAFS) JAWS: Joint Aquaculture/Agriculture Water Sharing Programs for Manure Management PROJECT DESCRIPTION This proposal for IFAFS Consortium Funding addresses five important objectives under Topic 5. Natural Resource Management (Program Area 14.3 Animal Manure Management): (a) development of rates and methods of land application of manure that are most suitable for a given watershed; (f) determination of water quality impacts of nutrients, pathogens, and other waste products, and the development of strategies to reduce such impacts, and the development of programs to educate the public on such water quality issues; (g) development and implementation of alternative waste treatment technologies; (h) development and marketing of value-added products from animal waste; and (j) development of alternative animal production systems. A. INTRODUCTION Aquaculture, the controlled culture of fish and shellfish, is an extremely large industry worldwide, with more than 55 billion lb produced annually. In the U.S., aquaculture has become a one billion dollar industry, providing nearly 15% of our seafood supplies. Aquaculture is an ecologically efficient means of providing seafood for American consumers that reduces fishing pressure on our limited wild fisheries resources and reduces our dependence on imports. Many recent technological breakthroughs in genetics, nutrition, and pathology have made aquaculture the fastest growing sector of the agriculture industry, expanding at an annual rate of 20%. A survey conducted by the USDA National Agriculture Statistics Service indicated that freshwater fish culture in the U.S. involves primarily catfish (581 million lb), salmon (110 million lb), trout (63 million lb), tilapia (12 million lb), and striped bass (9 million lb). However, aquaculture production in the U.S. appears to be reaching a limit, due to the finite supply of water. The industry also is threatened by environmental pollutants sometimes associated with the discharge of untreated fish farm effluents. As modern technologies for aquaculture develop, there are few locations in the U.S. where unutilized land and water resources are available for their implementation. U.S. agricultural operations already are utilizing nearly all of our water supplies that are not devoted to municipal and industrial activities. The few remaining untapped water resources often are designated for conservation and ecological preservation, or may have "wild river" status. No such limits are faced by foreign aquaculture companies, which have many advantages over U.S. producers. Many developing countries have tropical and sub-tropical climates in which large quantities of warm water are available for aquaculture. Also, land and labor costs are low and there are few environmental restrictions or limitations on drug usage. Imports of fish grown in Colombia, Costa Rica, Ecuador, Taiwan, China, and Indonesia have increased markedly as the foreign competition adopts U.S. culture technologies. In order for U.S. growers to compete against the strong Kent SeaTech Corporation IFAFS Proposal Page 2 advantages of foreign producers and expand significantly in the U.S., novel technologies and approaches will be required. Since its inception in 1972, Kent SeaTech Corporation has been conducting research to develop advanced technologies to improve the competitive position of the U.S. aquaculture industry. With federal research funding from the U.S. Department of Agriculture, the National Science Foundation, the U.S. Department of Commerce, and the NIST Advanced Technology Program, we have developed a variety of technological advances that are assisting the U.S. fish farming industry. Based on our preliminary research funded by USDA and the Advanced Technology Program, we believe that the solution to the problem of limited availability of water supplies for U.S. aquaculture development may involve a water sharing approach. Our studies indicate that high density aquaculture operations can be located in proximity to large agricultural operations, utilize the source water in a non-consumptive manner, treat it at minimal cost, and then deliver it to row crop farmers. This dual or multiple-use of limited water resources means that the two industries can share a single water source and may effectively double crop production by these techniques. When several innovative water treatment and recirculation technologies also are utilized, there may be a 300-400% increase in the total crop value yield per acre-foot of water consumed. There have been several previous attempts to increase productivity from water resources by combining aquaculture and agriculture. However, most of these have involved the stocking of small numbers of fish in existing irrigation canals as a secondary source of income for agriculture operations. As many of these operators have learned, fish culture is a difficult enterprise requiring a considerable amount of technical skill, and therefore these small, supplemental fish crop programs have not been overly successful. In contrast, what we propose is the combining of technologically advanced high density aquaculture technologies with modern row crop agriculture practices. Each industry is sophisticated enough that it requires professional management, but they can still share the water resource that they have in common to mutual benefit. The overall concept is illustrated below: Conceptual Flow Diagram Intensive Aquaculture Water Supply (Continuous, non-consumptive user) Low Cost Water Treatment and Recirculation System Irrigated Agriculture Concentrated Fraction (Intermittent, consumptive user) Easily-treated Fraction Agriculture also would benefit significantly by this approach. At the turn of the previous century, crop irrigation represented just 1% of all U.S. water use. However, as modern agricultural practices have developed, irrigation has grown rapidly and now represents 41% of all our water resource use in the U.S. More than 150 million acre-feet of water per year are devoted to irrigating crops. Nearly all of this water use occurs in the West, since 89% of all irrigated Kent SeaTech Corporation IFAFS Proposal Page 3 crops are farmed in the nine western water regions. The costs of irrigation water are substantial, varying from $6 to more than $400 per acre-ft. In many areas, local water costs are increasing and farmers are sometimes unable to operate at a profit. If techniques were available so that aquaculturists could utilize the source water and then pass it on to land farmers with no loss of volume or quality, the aquaculturists would be more than willing to pay a portion or even all of the farmer's water costs. The resulting increase in profitability for row crop farmers could be significant. Further, the fish manure that is present in aquaculture effluent is at relatively low concentrations and could provide an additional source of nitrogenous fertilizer for row crops. The water sharing concept appears to resolve significant problems facing both aquaculture and agriculture. Kent SeaTech has conducted preliminary research funded by the NIST Advanced Technology Program which indicated that partially treated aquaculture effluent applied to test plots of corn and lettuce was able to provide all the water flow required for irrigation and did not appear to have any adverse chemical effects. In these studies and in additional research funded by the USDA SBIR program, we are finding that constructed wetlands may serve as inexpensive water treatment systems to treat a portion of the effluent so that it can be recycled and reused in the aquaculture component to increase production, before it is released to the agriculture operation. The ability to recycle the water is an important part of the overall concept, since it allows the water flow to the agriculture component to be intermittent, as required by the row crop irrigation schedule, and yet allows continuous flow through the aquaculture component via recycling. We are using the term Joint Aquaculture/Agriculture Water Sharing (JAWS) to describe this overall concept. In this project, we propose to conduct research to develop and refine aquaculture/agriculture water sharing technology and to conduct education and extension activities that will develop and implement this technology in the western U.S. We will utilize a consortium approach to achieve these objectives, and have assembled an excellent team of cooperating researchers, educators, and extension experts to ensure that the technology becomes widely implemented. The research activities will take place at two locations: 1) the high density fish culture facility of Kent SeaTech Corporation in California, where the existing systems are being modified and retrofitted to allow the delivery of treated farm effluent to cooperating agriculture operations, and 2) a new high density fish culture facility to be developed by Kent SeaTech in conjunction with Vicksburg Farms, a modern agricultural operation in Arizona, which will be designed from the outset to utilize the most efficient water sharing technologies that we develop. The California studies will focus upon careful measurement of the effects of fish farm effluent on common row crops such as corn and lettuce, and will build upon initial work we conducted under funding from the Advanced Technology Program. The Arizona test facility will evaluate several new concepts that should result in increased water treatment and reuse capability, and will be specifically designed to assist in convincing agricultural interests that the water sharing concept will work in their application and result in significant cost savings. University extension experts in both states will work closely with the project to insure that the technology appeals to a broad base of small and large agricultural interests. Also, the Director of the USDA Western Regional Aquaculture Center will work collaboratively with the Consortium to encourage aquaculture extension specialists in all western states to promote this technology wherever it may be applicable. This combined research-verification-extension approach is Kent SeaTech Corporation IFAFS Proposal Page 4 exactly what will be required to convince agriculture operations of the significant benefits that could result from water sharing technologies. PLACEHOLDER FOR 3 COLOR PICTURES Project Objectives The overall goal of this project is to develop and promote the widespread implementation of cost-efficient methods of interfacing aquaculture and agriculture facilities so that existing supplies of valuable irrigation water can be shared by both industries to conserve resources, reduce environmental pollution, and increase profitability. In order to accomplish this goal, the Consortium will address the following eight Project Objectives: 1) To evaluate in large-scale field trials the suitability of aquaculture effluent water for irrigation of terrestrial agriculture crops. 2) To determine whether the primary waste products of aquaculture operations such as ammonia nitrogen, phosphorous, and fish manure are useful by-products that can be utilized by agriculture and simultaneously reduce effluent disposal problems of aquaculture. 3) To develop cost-efficient methods of aquaculture effluent treatment that will reduce pollution, permit multiple uses of existing water supplies, and leverage aquaculture production capacity. 4) To evaluate the use of constructed wetlands technology as an extensive method for potential use in aquaculture/agriculture water management to: a) treat aquaculture effluents to allow for recycling water to increase effective utilization of water resources; b) settle solids and concentrate aquaculture waste nutrients for delivery to agriculture; c) function as a buffer to modulate differences in water requirements between the needs for continuous use in aquaculture and intermittent use in agriculture. 5) To use the information developed during these studies to design, construct, and evaluate a prototype water treatment and sharing system of sufficient scale that the results will have commercial applicability and will be useful in convincing potential users of the feasibility of the concept. The prototype system will receive effluent from a high density fish culture system that Kent SeaTech Corporation IFAFS Proposal Page 5 will be provided to the consortium as in-kind match. 6) To conduct economic modeling studies using the results of the above research, in order to predict the cost-effectiveness of the integrated water sharing technologies at full-scale application. 7) To educate the farming community, water agencies, and general public regarding the economic and environmental benefits of water sharing technologies and the large opportunity for expanding aquaculture production without the need for developing any new water resources. 8) To conduct extension activities to promote water sharing strategies in the western U.S. Previous Research There has been relatively little research conducted on the sharing of water resources between aquaculture and agriculture. Most of the research that has been conducted has focused on the potential benefits involved in use of the nitrogen wastes in aquaculture effluent. While the value of the nitrogen released from fish farms may be significant, in our opinion the value of the water itself may be a much more compelling reason to develop sharing strategies. Also important is the role that water sharing could play in reducing the environmental impacts of aquaculture effluents that would otherwise be returned directly to receiving waters or percolate to groundwater. Westerman et al. (1993) estimated that the trout industry alone produces about 10 million kg of solid wastes annually. Another likely benefit is the storage function that a recirculating aquaculture and water treatment facility could offer, which could help to synchronize the intermittent water demands of row crops with the continuous water demands of high density aquaculture. Little research has been conducted concerning these important aspects of integration of aquaculture and agriculture. Use of Aquaculture Effluents in Agriculture. A review by Phillips et al. (1991) indicated that worldwide there is a relatively high water demand in aquaculture and a need to optimize water reuse by integration with agriculture. Some preliminary research to achieve integration has been conducted. The potential economic value inherent in fish farm effluent was described at a Aquaculture Engineering Conference held in Spokane, WA (Wang 1993). At the TVA National Fertilizer and Environmental Research Center, effluent from a conventional sewage treatment facility was used to irrigate agricultural crops (Reed 1988). They studied the role of plant species, hydraulic loading, ammonia removal, and maintenance and operating costs. The effluent from aquaculture operations is rich in nutrients that can be applied to agricultural irrigation. This practice has been used at low densities for over 5,000 years in Asia, where they have fully integrated rice-fish-vegetable production. Integrated aquaculture effluents have been used for crop irrigation in Europe for several decades (Rosenthal 1991). In Canada, researchers have utilized sludge from land-based salmon farms as agricultural fertilizer (Lystad and Selvik 1991, Bergheim et al. 1993). They indicated that trout farm solid waste appeared similar to livestock waste in levels of nitrogen, phosphorous, calcium, and magnesium, but had lower levels of potassium (Naylor and Moccia 1993). In Israel, fish farm effluent has been used in agriculture, and in the Philippines fish farm wastewater has been used to irrigate maize, rice, and vegetables Kent SeaTech Corporation IFAFS Proposal Page 6 to increase yield and profitability. Some of the most pertinent research in this field has been conducted by Dr. Kevin Fitzsimmons, a member of our proposed research team. Several evaluations of the use of aquaculture wastewater in agricultural irrigation were conducted at the Environmental Research Laboratory at University of Arizona, the Maricopa Agricultural Experiment Center, the Gila River Indian Reservation, and several private facilities (Fitzsimmons 1988). One agricultural operation they evaluated required 50-300 kg/ha nitrogen and 1.2 m of water when using conventional irrigation and fertilization techniques. However, when applying integrated water use strategies, the same agricultural operation required only 45 kg/ha nitrogen and 0.9 m of water. These studies indicated possible concerns with particulate waste plugging drip irrigation systems, the mechanics of delivering wastewater from the fish farm, and costs for extensive distribution systems. Nonetheless, wastewater from tilapia and catfish production operations in the Gila and Salt River Valleys was successfully used for experimental crop irrigation. Other research by Dr. Fitzsimmons and his associates demonstrated that water costs can be reduced for both fish and row crop farmers, and that farmers were able to reduce their chemical fertilizer use (Olsen et al. 1992, Olsen and Fitzsimmons 1994). Uneaten feed and fecal matter contributed organic compounds and nutrients. The nitrogen available in this effluent was calculated to be about 0.03 kg NH4-N per kg of feed fed to the fish. The research demonstrated that fish farm effluent could be applied successfully and that a portion of the required nutrients was supplied in the fish farm effluent. In these studies, farmers shared water costs with aquaculture and were able to save $123 per hectare ($50 per acre) in crop production costs (Yates et al. 1992). Other work by these investigators has addressed the potential for surface irrigation of cotton using aquaculture effluent (Olsen et al. 1993). In an experimental field study, Irving et al. (1992) compared commercial fertilizers to fish manure and found similar yields. Sweet corn had higher yields when provided with fish manure than when commercial fertilizers were used. At the LSU Cooperative Extension, scientists conducted research to integrate catfish and crawfish culture with traditional agriculture (Lutz, pers. comm.). At the University of the Virgin Islands, sludge from tilapia culture tanks has been compared to cow manure and liquid and granular inorganic fertilizer (Rakocy 1994). The yield for peppers irrigated with fish wastewater was comparable to yields obtained from application of commercial fertilizers. At the University of Georgia's Coastal Plain Experimental Station, researchers evaluated the use of catfish pond effluent in sprinkler irrigation of soybean and wheat crops (Ghate et al. 1994, Burtle and Ghate 1994). The results indicated that effluent from catfish ponds provided from 15 to 75% of the nitrogen needs of the plants. Other studies of the potential for using aquaculture pond effluent for irrigation in the southeastern U.S. were conducted by the USDA Southern Regional Aquaculture Center (C.S. Tucker, compiler 1998). This report concluded that although the results of crop studies were good, the effluent from ponds, which are operated at much lower densities than high density fish culture tank systems, was not sufficiently nutrient-rich to significantly reduce the use of fertilizers. Aquacultural effluents also have been studied for use in hydroponic systems. The results of most studies have indicated that the effluent should be Kent SeaTech Corporation IFAFS Proposal Page 7 augmented with additional nutrients to be useful. Adler et al (1996) designed a conveyor system to direct high phosphorus effluents to younger plants or seedlings, which are better at removing phosphorus than are older plants. Wetlands Water Treatment Systems. Constructed wetlands have been developed and tested for use in municipal sewage treatment in the U.S., Canada, and Europe. Several publics works projects to restore waterfowl habitat have resulted in the development of techniques to manage bulrush communities. More recently, this water treatment technology has been adapted to treat waste from industrial and agricultural operations. Constructed wetlands have been used to treat concentrated animal waste from livestock and poultry in recent years (Hammer 1993). These systems are low-technology and low-cost, and are more compatible with general agricultural practices. A relatively small amount of energy is required for their operation. In some applications, wetlands technology may be employed for less than 1/10th the cost of conventional sewage treatment systems. It often involves lower construction costs, lower maintenance costs, simpler designs, and lower pumping heads. Also, it is often suitable for multiple objectives, such as waste treatment, recirculation, and reuse applications. Two of the principal research centers for this research are Auburn University's Sand Mountain Agricultural Experimental Station in Alabama, and Mississippi State University's Pototoc Experimental Station. At the Auburn facility, wetlands have been used to treat hog production effluent, and at the Mississippi facility, dairy farm effluent is treated (Hammer and Bastian 1991, Hammer 1993). Wetlands have been used effectively to provide primary and secondary treatment of effluents to remove the organic load (BOD) and suspended solids (TSS), and eliminate pathogens. The principal process to reduce nutrient levels involves ammonification, which requires an oxidized environment. Effluents are effectively treated to meet secondary discharge standards where BOD and TSS levels must be less than 30 mg/l. Constructed wetlands have been used to treat swine manure at Muscle Shoals, Alabama, since the late 1970's (Maddox and Kingley 1991). Livestock waste also has been treated with wetlands at Sand Mountain Agricultural Experimental Station (Hammer et al. 1993). Similar results were observed in the treatment of dairy and swine waste, where there was a 90% reduction in total nitrogen and 80% removal in total phosphorus (Surrency 1993). Zachritz and Jacquez (1993) at the Southwestern Technical Development Institute of Northern Mexico State University evaluated a surface flow wetlands planted with Scirpus californicus. They determined that a four day retention time was required for adequate nitrification. Wetland studies at the facility at Gustin, California, using loading levels of 18-116 BOD/ha/day (16-104 lb/ac/day), showed effective removal of 93% of the BOD. Surface flow systems have been used to polish secondary effluent prior to discharge into Humboldt Bay in Arcata, California (Gearheart and Higley 1993, Gearheart et al. 1991). There has been little previous work on the use of wetlands to treat aquaculture effluent. Our own research in this field is described in the next section. In addition, Axler et al. (1996) conducted Kent SeaTech Corporation IFAFS Proposal Page 8 studies on the use of constructed wetlands for treating of aquaculture wastes in northern Minnesota. Summerfelt et al. (1996) conducted research on aquaculture sludge removal and stabilization within created wetlands. A review by the Southern Regional Aquaculture Center (1998) provided examples where constructed wetlands have been used for the treatment of aquaculture effluents. The authors stated that the space requirements could be prohibitive in some cases and recommended a hydraulic residence time of four days. Schwartz and Boyd (1995) considered the use of constructed wetlands for the treatment of channel catfish pond effluents. Fish Manure. Fish manure contains approximately 4% total nitrogen and 90% organics. It is high in nitrogen and phosphorus, and low in potassium and trace elements. The components of salmonid hatchery waste in Ontario have been quantified for fish receiving a diet with 80% digestibility, at a feed conversion of 1.2:1 where total suspended solids were 300 g/kg feed per day, dissolved phosphorus 2.2 mg/l, and total ammonia 38.3 mg/l (Castledine 1986). Summary data have been published on the average water quality of salmon hatchery effluent in Washington, where suspended solids were 7.0 mg/l, BOD 5.4 mg/l, ammonia 0.5 mg/l and phosphorus 0.1 mg/l (Liao 1970). Measurements of effluents from freshwater fish culture facilities indicated varying levels of waste compounds: ammonia 1-3 mg/l, nitrates 1-5 mg/l, total nitrogen 1.5-6 mg/l, phosphate 0.10.5 mg/l, total phosphorus 1-2 mg/l, and total filterable solids 5,00-1,000 mg/l. Similar concentrations were recorded for catfish pond operations in Mississippi (Pruder and Tchobanoglous 1989). They reported the following measurements of pond water quality in summer: total nitrogen 5.6 mg/l, nitrate/nitrite 0.2 mg/l, total ammonia 0.4 mg/l, total phosphorus 0.8 mg/l, and total solids 500 mg/l. They also noted that an important treatment concern is removal of solids, which is usually accomplished in sedimentation tanks through gravity settling. The amount of suspended solids to be treated greatly depended on the amount and degree of management control of feeding operations. The volume of solids produced ranged from 2001,000 kg per metric ton of fish produced, and represented about 300 g per kg of feed. About 9 g of phosphorus is produced per kg of feed, with 2/3 of the phosphorus bound in the solids and 1/3 in the soluble fraction. Regulatory Issues. The aquaculture industry is becoming increasingly concerned that overregulation will restrict its growth (Batterson and Piedrahita 1996). For some time the EPA has been concerned primarily with particulate wastes in the effluent from fish culture facilities and has required that particulate settling be performed prior to discharge to the environment. More recently however, there are more stringent guidelines being promulgated that also will address the biostimulants (primarily nitrogen and phosphorus) that are present in aquaculture effluent. These compounds are more difficult to remove, even though they are present at low levels compared to traditional municipal or industrial waste streams. Biostimulants can cause eutrophication of receiving waters even at the low concentrations found in aquaculture effluent. Intensive (high density) aquaculture facilities are now required by the EPA to meet discharge standards set by NPDES permit (EPA “Notice of Proposed Effluent Guidelines Plan” Section V.B.2.g – Fish Hatcheries and Farms 1998). Further, there is mounting pressure from environmentalists calling for increased regulation of the industry. The Environmental Defense Kent SeaTech Corporation IFAFS Proposal Page 9 Fund recently published a report cautioning that aquaculture discharges have the potential to damage the environment if the industry is not observed and regulated (Goldberg, and Triplett 1997). These issues will affect all aquaculture operations to a significant degree. Methods of reducing the concentrations of biostimulatory compounds such as ammonia from levels of 3-5 mg/l down to levels of 0.5 mg/l will be expensive and require more effort than systems that reduce municipal effluent from 25 mg/l down to 2-3 mg/l. Our work with constructed wetlands offers a promising means of meeting the target objectives at low operating cost. Even more useful will be water-sharing technologies that would allow the application of the nitrogenous waste directly on field crops without expensive treatment required. Previous Research By Kent Seatech Corporation In previous federally-funded research, Kent SeaTech has developed several advanced aquaculture methods in which fish are held at high densities in intensive raceways or tanks and the culture water is recycled through a series of intensive and extensive systems for water treatment. Under a cooperative funding agreement from the NIST Advanced Technology Program and with substantial in-house matching support, we conducted research to develop low-cost water treatment systems that would provide cost effective and efficient methods of removing particulates and metabolites from fish farm effluents. We investigated the potential for adapting the wetlands method of sewage treatment to aquaculture wastewater treatment. Our wetlands treatment components consist of shallow lagoons in which plant and bacterial populations are managed in order to maximize the removal of ammonia and other environmentally damaging compounds. The managed wetlands wastewater treatment technology can be applied to many forms of land-based aquaculture production, including ponds and open or recirculated tank culture systems. In addition, we conducted research on the development of a nitrifying reactor process to remove nitrogenous compounds from aquaculture effluents. We also conducted preliminary trials concerning the reuse of the treated effluent both for additional aquaculture production and for irrigation of agricultural crops. The results of these studies are summarized in the following sections. Constructed Wetlands. Traditional constructed wetlands designed for treatment of domestic wastes generally have long residence times, employ sub-surface gravel beds, and discard the water rather than recycle it. Our research on wetland ponds designed for use in treating aquaculture effluents involved studies of the effects of pond size, depth, macrophyte species, plant thinning protocols, aeration, and hydraulic retention time (HRT). Initially we conducted a research effort with in-house funds to recirculate a portion of the effluent water from our striped bass culture facility through a series of open-water treatment ponds. This facility is located near Palm Springs, California, and currently produces over 1.3 million kg (3.0 million lb) of striped bass annually. The objective of our preliminary research was to evaluate whether a fraction of the water flow could be nitrified and reused for additional production within an intensive striped bass rearing system. Constructed wetland ponds were shown to be capable of reducing total ammonia to 0.25 -1.0 mg/l, at input flows of up to 4,500 liters/minute (1,200 gpm) of untreated water. Kent SeaTech Corporation IFAFS Proposal Page 10 Based on the preliminary success we achieved in utilizing open-flow ponds to treat aquaculture effluent, we began a program to develop constructed wetlands for a larger study of water treatment and reuse. These systems appear to offer a simple, inexpensive, water treatment alternative for fish farms that are located in areas with sufficient available land. Constructed wetlands used for sewage treatment consist of shallow earthen ponds planted with rooted aquatic macrophytes such as cattails, bulrush, reeds, etc. Current designs suggests multistage lagoon patterns (Hammer 1993) and the use of rectangular ponds (Steiner and Freeman 1991) to reduce accumulation of organic matter and effectively reduce nutrient levels. Support for this research was provided by the NIST Advanced Technology Program. The initial studies indicated that the best species of bulrush for use in this application was the California bulrush, Scirpus californicus, and that the optimal hydraulic residence time for nitrification of aquaculture effluent could be as short as 0.5-1.0 days when supplemental aeration was provided, thus allowing the wetlands to provide more efficient aerobic nitrification. This significantly reduces the area required for wetlands construction. Our initial studies indicated that the economics of wetlands treatment appeared favorable, although seasonal temperature variations affected the efficiency of the system. The wetlands system also appeared to function best as part of a sequence of inexpensive treatment steps, as described below. Sequential Treatment Process. Our ATP research produced several promising treatment methodologies that functioned best as a series of sequential treatment steps in an overall process we termed the SMART-WetlandsTm process. First, aquaculture effluent was delivered to a long, concrete Solids Removal Raceway stocked with high densities of tilapia, a detritivorous fish that was shown to have high potential as a means of removing suspended solids and particulate matter that were present in the effluent. This step removed approximately 30% of the suspended solids before they could decompose and create more harmful and toxic compounds such as ammonia. After exiting the raceway, the effluent water was supplied to an enhanced form of nitrifying reactor we developed, called the Suspended Media Ammonia Removal Technology (SMART) system. This water treatment component consists of a large oval concrete tank in which the water is circulated by means of a large hydraulic paddlewheel. The water column contains a number of polyurethane foam cubes that provide a large amount of well-aerated surface area for the growth of nitrifying bacteria. These bacteria break down ammonia, the most common and most toxic compound present in fish culture effluent, to less toxic nitrogenous compounds such as nitrate. In our prototype systems, the SMART system was capable of removing at least 40% of the total ammonia present. The third step in the sequential treatment process involved the delivery of the effluent to large, shallow earthen ponds that were planted with mature bulrush plants (Scirpus sp.). A series of studies were conducted to determine the optimal species, pond depth, plant density, aeration rates, and water residence time for the effluent flowing through these treatment ponds. This polishing step was shown to be capable of removing at least 40% of the remaining ammonia and nearly all of the remaining suspended solids. Kent SeaTech Corporation IFAFS Proposal Page 11 Water Reuse Program. The final step we evaluated was the delivery of a portion of the effluent to nearby cooperating vegetable farmers. Sharing of water resources in this manner would allow the aquaculture operation to utilize new water first, which is important because most aquaculture species require very clean water, whereas most plants will tolerate and even benefit from a supply of nitrogenous compounds. We conducted a small test program with nearby vegetable growers to evaluate the usefulness of aquaculture effluent in the irrigation of several row crops such as corn and lettuce. A supply line was installed and the cooperating growers planted several test plots that received aquaculture effluent, which were compared to identical test plots irrigated with well water. Laboratory analyses of the plants indicated that there were no significant differences in the quality of crops produced by these two methods. Further, in these preliminary trials, the fertilizer present in the effluent provided some advantages to the growers. Our studies indicated that yields for the crops were increased by 10%, and a 15% savings in fertilizer costs was realized due to the nitrogen fertilizer available in the recycled water. These studies were encouraging and indicated that effluent from the fish production tanks is desirable for use as irrigation water, not only as an alternative source of water, but also as a partial source of fertilizer. Pondway PAS Systems. We also have conducted USDA-funded research in cooperation with Dr. Dave Brune of Clemson University to evaluate the use of wetland ponds in Partitioned Aquaculture Systems (PAS). PAS facilities involve a low energy, intensive-extensive culture approach in which the fish are held at high density in one section of a pond or raceway and the water is passed through the fish chamber and then into larger sections where traditional pond nitrification can take place. In Dr. Brune's research on PAS systems, the main nitrification activity is provided by managed populations of unicellular algae, whereas in our Pondway form of the PAS, the pond zone is planted with vascular plants such as Scirpus, and bacteria on the submerged surface plant surfaces perform much of the nitrification. These studies are providing useful data on wetlands efficiency and interface well with the water sharing research proposed here. B. RELEVANCE AND SIGNIFICANCE Relationship of Objectives to IFAFS Goals We believe this project conforms excellently to the objectives of the IFAFS program. The overall goal of this project is to develop and promote the widespread implementation of costefficient methods of interfacing aquaculture and agriculture facilities so that existing supplies of valuable irrigation water can be shared by both industries to conserve resources, reduce environmental pollution, and increase profitability. This conforms very well with the overall goal of the IFAFS program, which according to the authorizing legislation, is to focus upon "critical emerging agricultural issues related to 1) future food production, 2) environmental quality, or 3) farm income". Also according to the authorizing legislation, consortium projects such as ours should receive priority, since they are "multi-disciplinary projects that integrate agricultural research, extension, and education", and therefore offer the "greatest potential to produce and transfer knowledge directly to end users". Kent SeaTech Corporation IFAFS Proposal Page 12 Our consortium addresses five important IFAFS objectives under Topic 5. Natural Resource Management (Program Area 14.3 Animal Manure Management): (a) development of rates and methods of land application of manure that are most suitable for a given watershed; (f) determination of water quality impacts of nutrients, pathogens, and other waste products, and the development of strategies to reduce such impacts, and the development of programs to educate the public on such water quality issues; (g) development and implementation of alternative waste treatment technologies; (h) development and marketing of value-added products from animal waste; and (j) development of alternative animal production systems. The RFP also specifically mentions aquaculture as one of the five animal groups that should be the focus of proposals under Program Area 14.3, and further suggests that projects to develop methods of managing manure by the use of wetlands are encouraged. Kent SeaTech Corporation has received 15 federally-funded aquaculture research grants previously, and therefore should qualify for IFAFS funding as a private research organization "with an established and demonstrated capacity to perform research and that (1) conducts any systematic study directed toward new or fuller knowledge and understanding of the subject studied, or (2) systematically relates or applies the findings of research or scientific experimentation to the application of new approaches to problem solving, technologies, or management practices; and (3) has facilities, qualified personnel, independent funding, and prior projects and accomplishments in research or technology transfer." Significance of Activity There is little doubt as to the economic importance of research in this field. Almost every major review of aquaculture has described the critical need for improved aquaculture water treatment and water integration systems if this new industry is to continue to expand in the U.S. The National Aquaculture Act and the revised National Aquaculture Plan highlight the importance of this area of aquaculture research and development. The 1994 National Agenda for Aquaculture and the Environment describes the critical need to conserve water and utilize wastes in integrated systems which combine terrestrial agriculture and constructed wetlands. The Congressional Joint Subcommittee on Aquaculture and the National Research Councils promote a "national agenda to encourage the development of advanced aquaculture technologies and environmentally sound, renewable resources", as part of the Presidential Initiative on Sustainable Development. The USDA Regional Aquaculture Centers also emphasize the importance of aquaculture waste management, and encourage research "to characterize waste, evaluate technologies, develop the best management practices, and promote technology transfer" (Broussard, unpubl.). Similar emphasis is placed on the topic by the Cooperative States Research, Education, and Extension (CSREES) Program, and the Sustainable Agriculture Research and Education Program (SARE) (Rasmussen pers. comm.). Aquaculture waste management has been declared a National Need having top priority by the Agriculture in Consort with the Environment (ACE) Program, a joint effort of the USDA and the EPA. Industries to Be Assisted. The development of successful technologies for agriculture uses of Kent SeaTech Corporation IFAFS Proposal Page 13 aquaculture waste water will be of benefit to many farm operations in the West, since they will benefit from sharing water costs and the reduced demand on the limited canal water and groundwater supplies. Those farms that decide to implement the sharing concepts we develop should enjoy increased profitability and production. In addition, the technology and concepts we are proposing to develop can be implemented in many other areas throughout the U. S. The Southwest is one of the most productive agriculture areas in the U. S. It also has a relatively good supply of well water and Colorado River water, which is ideal for the culture of many species of fish. There are many agricultural operations in this area which could benefit from additional sources of irrigation water. Agriculture operations that are physically close to existing fish culture facilities will be the first to benefit from the development of this new technology, but the results of our research and feasibility analyses also will be made available to all agricultural operations through the Cooperative Extension efforts of the University of Arizona, and the Davis and Riverside campuses of the University of California. All interested farmers will be encouraged to tour the research facilities and determine for themselves whether the water reuse concepts we develop would be beneficial to their specific applications. In addition, we will work with the McMullen Water Conservation District to encourage participation by as many agriculture operations as possible, and with Dr. Kenneth Chew, Director of the Western Regional Aquaculture Center, who will coordinate extension activities with all aquaculture extension specialists in the western states. Potential Increases in Agriculture Production. In many areas that are otherwise suitable for increased agriculture production, the major factor preventing increased yields is the lack of additional water supplies. If suitable technology could be developed so that aquaculture effluents could be delivered to nearby agricultural operations, it is possible to estimate the substantial theoretical gains in agricultural production which might be achieved. For example, a major user of water in the Northwest is the rainbow trout culture industry. If the effluent from just 10% of the trout farms in the Northwest could be diverted to irrigation of vegetable crops, there would be sufficient irrigation water made available for 95,000 acres of vegetables, worth nearly $400 million annually. It is also possible to estimate the potential increased profitability that agriculture could achieve if aquaculture companies are allowed to build new facilities near the water source points of existing irrigated fields and use the water prior to delivery to the row crops. In the table below, if we assume that the new aquaculture enterprises would be willing to pay 50% or 100% of the water costs, the benefits to the agriculture operation are $41/acre and $82/acre, respectively. In many cases, this could represent a doubling of the profits made by the growers. Kent SeaTech Corporation IFAFS Proposal Page 14 Potential In creas e in Pro fitab ility for Field Crop Agriculture Op erations Employin g Water Sh arin g Mode of Op eration Av erage Av ereage An nual An nual Income Increased Pro fit Cos t of Water Usage Water Cos ts from Field from Water Water-$/AF -AF/yr - $/yr Crops -$/ac/y r Sharing -$/ac/yr Trad ition al Op eration (ag ricu lture pays all $20.50 4.00 $82.00 $733.00* water co sts ) Water Sharing 50:50 (sp littin g of water $10.25 4.00 $41.00 $733.00 co sts ) Water Sharing 100:0 (aq uacultu re p ays all $0.00 4.00 $0.00 $733.00 water co sts ) *-Average an nual in come from irrigated field cro ps in Californ ia durin g 1995. $0.00 $41.00 $82.00 Similar projections can be made for the Southwest, other major farming areas in the Southeastern U.S., and most agricultural areas of the country. The exact amount of potential increase in crop production and farm profitability will depend on the species of fish, types of land crops that are cultivated, and the local costs for irrigation water. Potential Increases in Aquaculture Production. Substantial gains also could be made in aquaculture production if techniques are developed to allow fish farmers to utilize irrigation water currently used for land crops. This use would be upstream of the existing agriculture operations and would not degrade the water quality or significantly reduce the quantity available. Following the assumptions used in the theoretical analysis below, it can be shown that if agriculture irrigation water were to be used in fish culture prior to application to row crops at just 4% of farming sites in the United States, the amount of seafood produced through aquaculture could double, from about 900 million pounds annually to nearly two billion pounds. The potential increase at Vicksburg, AZ alone could be as much as 10 million pounds annually. Region Vicksburg Coachella Arizona California 6 Southwest States United States Irrigated Land (1000 acres) 6 78 1,090 9,480 16,500 57,900 Water Usage (1000 ac-ft/yr) 25 314 6,300 32,400 62,200 150,000 Potential Fish Production (million lb/yr) 10 43 860 4,400 8,500 20,500 Potential Value $10M $40M $0.8B $4B $8B $20B An increase in aquacultural production of this magnitude in the rural areas of the Southwest would have a dramatic effect on the high unemployment rates typically present in this area. In general, the labor involved in producing one pound of fish represents about $0.25 to $0.50 of the total costs of production. Therefore, the additional employment required to cultivate these additional crops would be about 10 to 20 million dollars annually, which represents approximately 1,500 to 3,000 additional full-time positions. The ultimate commercialization of the technology will provide a range of broad-based benefits to the country. The technology will assist in lowering production costs, which will stimulate increased aquaculture production. Higher levels of production result in increased supplies to meet the demand for nutritious seafood products and also can result in potentially lower prices for consumers. Increased production also will help to reduce our large import trade deficit for Kent SeaTech Corporation IFAFS Proposal Page 15 seafood ($6.9 billion annually). Widespread use of these concepts in aquaculture will provide more domestic employment, more conservation and integrated use of our limited water supplies, reduced pollution, and increased conservation of our wild fisheries stocks. This technology could provide the key for successful integration of intensive, non-consumptive aquaculture with crop agriculture. Approximately 85% of all water distributed in the western U.S. is used by agriculture. Integration of traditional agriculture with aquaculture could provide tremendous expansion opportunities for the aquaculture industry, allowing U.S. aquaculturists to compete more effectively with foreign supplies and the capture fisheries. Cooperating farming operations also will benefit, as a result of reduced operating expenses resulting from the sharing of water resources, reduced fertilizer requirements, and increased crop yields. SharingPrograms Using Imported Canal Water. In California alone, over 4.4 million acre-feet of water is imported annually, primarily for agricultural use. Temporary diversion of even a small portion of this resource through aquaculture facilities would result in the production of many millions of pounds of seafood. In fact, this known, existing water resource is the equivalent of the water availability advantage held by foreign competitors in tropical nations. Wide-scale implementation of aquaculture/agriculture water sharing programs provides a realistic means for U.S. aquaculturists to overcome some of the major advantages held by overseas competitors. C. APPROACH (Not Done-Need Input from each investigator to fill in outline) We will design, implement and conduct several research studies needed to determine the most effective means of using irrigation supply water in aquaculture and agriculture applications. At the conclusion of the research, economic modeling studies will be done to predict the effects of the multiple-use concept on the profitability and production costs of both the fish farming and crop farming components. The final results and recommendations will be made available to all interested parties using a variety of methods to stimulate the implementation of the technology throughout the West.. Technical Research Activities (Just some preliminary ideas) 1) Development of research facilities a) Modifications to California research facility (retrofitted water sharing) b) Construction of Arizona research facility (designed specifically for water sharing) (research on fish culture tanks and components are not part of project - these will be provided at no cost by KST) 2) Effects of aquaculture effluent on crop quality and soil chemistry 3) Development of water treatment components a) Particulate Removal - tilapia channel, bead filter, settling in wetlands b) Primary Nitrification - SMART system, other media c) Secondary Nitrification - wetlands, seasonal effects, aeration, harvesting Kent SeaTech Corporation IFAFS Proposal Page 16 d) Efficient water transport techniques between components e) Wetland underdrains to separate particulates 4) Development of JAWS technology a) Develop methods to interface continuous AQ w intermittent AG 1) Wetlands as "storage" or "water use buffer" 2) Continuous Organic Fertigation vs Intermittent Chemical Fertilization (may be better for groundwater, less leaching) Education Activities 1) University Graduate Student Projects 2) University Class Lectures 3) Development of Secondary Education Curriculum 4) Facility Tours Extension Activities 1) Presentations to Industry Organizations 2) Agriculture Publications and Press Releases 3) Aquaculture Publications and Press Releases 4) Development of Video Presentations 5) Development of Websites 6) Facility Tours 7) Water District Contacts Synchronizing intermittent agriculture water demands with continuous aquaculture demands. One potential problem with the interfacing of high density fish culture and field irrigation for water sharing is the differences in daily and seasonal demand for water. Whereas fish culture tanks need a constant new supply of new makeup water (or treated water that is low in ammonia and other nitrogenous compounds), the water requirements for field crops will vary according to several factors. We will investigate a variety of techniques that will bring the water requirements of the two industries into conformance. The least difficult is the selection of growing areas and specific crops that can be cultivated year-round. In the southwest, several crops are farmed in this manner. The disadvantage of this approach is that the technology may not be as applicable in colder climates. Another relatively simple method of synchronization is the use of the wetland ponds to physically store water volume, until the crop irrigation schedule requires it. However, due to the high volumes involved, this method probably would not provide more storage that what would be needed to accommodate daily fluctuations in water demand. We also will evaluate the use of the wetland treatment component as a storage method that could allow the flow to the field crops to be turned off, but still allow recycling to provide clean water for the fish culture system. In this application, storage does not mean a simple storage of the water volume, but means that the nitrogenous wastes could be "stored" in the wetland component Kent SeaTech Corporation IFAFS Proposal Page 17 until the field crops are able to receive the concentrated flow. In our previous research, it appeared that settled particulates may be allowed to settle in the upper zones of a wetlands pond and may not decompose (and create additional ammonia loading) unless the warmer water temperatures of summer are reached. Staging the release of wetland water at these times could shunt a high portion of the ammonia to the irrigated fields. We will evaluate this concept, and also determine whether a series of perforated underdrain pipes located beneath the wetlands may be useful in capturing this more concentrated flow and routing it to the fields. Another method of synchronizing water use that we will evaluate is varying the feed level supplied to the high density fish tanks to conform with the water usage capability of the field crops. This method will undoubtedly work to reduce or eliminate makeup water requirements for extended periods of time and we have used it successfully in actual commercial production. Another technique to match water requirements would be to treat the effluent sufficiently to allow direct r4elease to the environment. This approach might be most logical when the field crops are receiving enough rainfall that no irrigation is required. In this research we will determine whether these methods, in combination with other synchronization techniques described above, provide solutions that are economically justifiable for the aquaculture operator. Economic Modeling. In several previous federally funded studies, we have developed methods of utilizing computer modeling techniques to project, the effects of full-scale implementation of aquaculture technological innovations. Components of the models address the anticipated effects of new farming concepts on production capacity and on the overall economics of the operation. Using preliminary estimates of the efficiency of the various proposed culture and water reuse methods, we will use these modeling techniques to extrapolate the results to full-scale commercial operation, and conduct an analysis of the costs involved in these methods of culture. The analytical techniques to be used involve Monte Carlo risk analysis, which provides a range of probable outcomes, and calculates the probabilities associated with the estimates, as opposed to the more common single-value prediction technique. These estimates and cost predictions for the test facility design will be compared with agriculture and aquaculture separately, so that recommendations can be made regarding the overall benefits of incorporating these technologies in future commercial-scale facilities. Kent SeaTech Corporation D. TIME TABLE IFAFS Proposal Page 18 Kent SeaTech Corporation IFAFS Proposal Page 19 E. EVALUATION AND MONITORING 1) Evaluation and Monitoring of Project Results During the first quarter of the project we will meet with the consortium members to develop a series of performance criteria and target objectives that will be used to indicate success in each topic area. In the case of the scientific research objectives, such criteria will take the form of specific water treatment efficiencies, filtration loading parameters, and allowable effluent nitrogen concentrations. However, the projected capital and operating costs of each component when implemented at commercial scale also must be considered. We have developed and utilized several economic projection models successfully for this purpose on previous contracts. Some of the models utilize Monte Carlo iterative modeling techniques to predict the most likely costs of commercial-scale operation of system components, and also can associate probability estimates with the predictions. In this manner for example, we may be able to state that the likelihood that the proposed technology will result in nitrogen removal techniques less expensive than traditional municipal treatment technology is 75%. The success of less-quantifiable objectives such as our education and extension efforts will not be as convenient to measure. We will work with the consortium members to develop a series of targets, including the development of teaching curricula, training of graduate students, preparation of promotional materials, etc., that will be used as indicators of success for these activities. The ultimate success of the entire project will be the establishment of full-scale aquaculture/agriculture partnerships that put the water sharing technology we develop to commercial use. We will develop a program of periodic follow-ups by extension experts who will re-contact potential users periodically after they are exposed to the technologies we develop, so we can determine the degree to which the concepts developed by this grant are being put to commercial use. 2) Evaluation and Monitoring of Consortium Administration The consortium members will be consulted regularly to ensure that the administration of each component of the research, education, and extension efforts is meeting their requirements. A Consortium Steering Committee, consisting of three Principal Investigators who are not employed by the Lead Institution, will meet as needed to provide guidance regarding any changes in administrative policies that would improve the quality of the program outputs. Also, to ensure that all consortium members are able to participate at their required levels, funding requirements already have been calculated by each member in a detailed budget proposal. These budget amounts will be passed through to the Contracts and Grants Office of each consortium member, on a no-additional-cost basis. These amounts will not be decreased without the approval of 1) the consortium member affected by the budgetary change, and 2) the USDA IFAFS Project Manager (if required). F. COLLABORATIVE ARRANGEMENTS This multi-disciplinary project consists of research, education, and extension activities to be Kent SeaTech Corporation IFAFS Proposal Page 20 carried out in the western states, primarily in California and Arizona. In addition, we have included researchers on the East Coast with expertise in certain required fields and USDA extension experts based in Washington state. These professionals will participate in the project through a consortium arrangement, with Kent SeaTech Corporation serving as the lead institution. The primary responsibilities of each consortium member are described below: Consortium Members 1) Kent SeaTech Corporation, San Diego, California (Lead Institution) Principal Investigators: Mr. James M. Carlberg and Mr. Jon C. Van Olst Principal Investigators: Mr. Michael J. Massingill and Mr. Rodney J. Chamberlain Responsibilities: As the consortium lead institution, the company headquarters of Kent SeaTech Corporation will coordinate all research, education, and extension activities, provide technical and financial accountability functions for consortium members, and interface with IFAFS Project Leaders to ensure that all project objectives and reporting requirements are met. Kent SeaTech Corporation also will provide substantial matching funds for the project and will purchase or construct many of the capital assets required to conduct the research. Kent SeaTech Corporation scientists also will be a part of the consortium research team and will conduct experiments at the Kent SeaTech Aquaculture Research Facility in Coachella Valley, California, to develop efficient methods of treating effluent from large-scale high density aquaculture facilities so that it can be recycled and reused in aquaculture and delivered to cooperating agriculture operations. Concurrently with this research on water treatment components to be conducted in southern California, Kent SeaTech Corporation will construct a high density fish culture system and a water-sharing research facility in western Arizona, which will be interfaced with large cooperating agriculture interests. This facility will be located between the existing irrigation water supplies and large agriculture crop fields and will be used to develop and evaluate water reuse and sharing technologies. The combined aquaculture/agriculture operation will serve as a research and education facility to develop, promote, and implement water-efficient multiple-use strategies in the southwestern U.S. 2) University of Arizona, Tucson, Arizona Principal Investigator: Dr. Kevin Fitzsimmons, Dept. of Soil, Water, and Env. Science Principal Investigator: Dr. Jeffrey C. Silvertooth, Plant Sciences Department Responsibilities: Scientists from the University of Arizona will direct the educational and extension aspects of this research program. University research projects on water quality, soil chemistry, agriculture crop yield and quality, and other related topics will be conducted by university researchers and graduate students as part of their thesis topic research programs. The Arizona State Cooperative Extension Service will provide extension services to the agricultural and aquacultural communities, encourage potential users to visit the site, and educate the public in regard to the need for water sharing programs. 3) Clemson University, Clemson, South Carolina Kent SeaTech Corporation IFAFS Proposal Page 21 Principal Investigator: Dr. Dave E. Brune, Dept. of Agricultural & Biological Engineering Responsibilities: Agricultural and aquacultural engineers at Clemson University will assist in developing water treatment technologies and manure management processes that are appropriate for use in water sharing applications between aquaculture and agriculture. They also will cooperate in developing the required experimental design and testing protocols that will be used to determine the treatment efficiency and cost-effectiveness of the overall approach. 4) University of California Cooperative Extension Service Principal Investigator: Dr. Fred Conte, Aquaculture Extension Specialist, UC Davis Principal Investigator: Mr. Jose L. Aguiar, Farm Advisor, UC Riverside Responsibilities: Aquaculture and agriculture extension activities in California will be conducted by the University of California Cooperative Extension Service, which will educate the public in regard to the need for multiple use programs and develop statistical data to predict the potential value of water sharing technologies to aquaculture and agriculture users in California. A portion of this work will involve the writing of articles for publications such as Desert Ag Notes and the California Farm Bureau Newsletter. The Extension Service also will conduct research to accurately quantify the benefits and problems involved with the use of aquaculture effluent on agriculture crops, and consider the possible effects (such as pesticide overspray problems) of the agriculture operation on the aquaculture components. 5) McMullen Valley Water Conservation and Drainage District, Vicksburg, AZ Principal Investigator: Mr. James D. Downing, P.E. Responsibilities: Officials of the McMullen Valley Water Conservation and Drainage District (MVWCDD) in Arizona will assist in developing cost-effective water distribution systems and strategies for resolving differences in daily and seasonal water demands between aquaculture (a continuous non-consumptive user) and agriculture (an intermittent consumptive user). They will also evaluate the resulting water quality effects of proposed fish “manure” treatment systems, including particulate removal, nitrification, and constructed wetland systems, and develop statistical predictions regarding the usefulness of this approach for the agriculture industry in Arizona. Mr. Downing and the MVWCDD also will provide a means of contacting additional potential users throughout the state to inform them of the opportunities for water cost savings made available by implementation of this technology. 6) Vicksburg Farms, Vicksburg, Arizona Principal Investigator: Mr. R. O. Cramer, General Partner Responsibilities: Vicksburg Farms will conduct studies to evaluate the advantages and problems involved with the use of aquaculture effluents on agricultural row crops. They will provide several test plots that will receive effluent of varying concentrations, and will observe and quantify the effects on crop quality, crop yields, operational impacts, and ultimate water and fertilizer cost savings. Kent SeaTech Corporation IFAFS Proposal Page 22 7) USDA Western Regional Aquaculture Center, Seattle, WA Principal Investigator: Dr. Kenneth Chew, Director Responsibilities: Dr. Chew will coordinate the efforts of aquaculture extension offices throughout the western states to promote the adoption of this technology, if it is proved successful. Cooperating Investigators and Consultants 1) Mr. Jeff Percy, President, Ocean Mist Farms, Coachella, California Responsibilities: Ocean Mist Farms has offered to cooperate with the research program and will receive treated fish farm effluent, raise several test crops of corn and lettuce, and work with Extension Service scientists to determine the quality of the crops, the effects of effluent on the soil in the test beds, and other related production statistics. 2) Mr. Mart Nickerson, Prime Time Farms, Coachella, California Responsibilities: Prime Time Farms has offered to cooperate with the research program and will receive treated fish farm effluent, raise several test crops of corn and lettuce, and work with Extension Service scientists to determine the quality of the crops, the effects of the effluent on the soil in the test beds, and other related production statistics. 3) Dr. John Menke, St.Gregory College Preparatory School, Tucson Arizona (WET Project) Responsibilities: The Water Education for Teachers (WET) Project is a joint education and extension project of the U.S. Bureau of Reclamation and the University of Arizona. Dr. Menke will develop an education curriculum at the high school level and will develop educational exercises for science students that will promote an understanding of the need to conserve and share water resources throughout the Southwest. Some aspects of this work will be performed using a new wet lab facility being constructed at the school, which will allow students to conduct research on the effects of several source water types on fish culture systems and plant species receiving fish tank effluent. G. NEED FOR CONSORTIUM APPROACH This project has several important and ambitious objectives. While the research component to develop new technologies to allow multiple use of water resources is of extremely high value, on a practical level there must be a coordinated plan for implementation of the new technologies if the aquaculture and agriculture industries are going to benefit through cooperation. Currently, these industries co-exist with little communication or overlap in infrastructure, and do not possess convenient means to learn to work together toward mutual objectives. The wellestablished agriculture industry has considerable influence nationwide, but previously has not viewed the newly-developing aquaculture industry as much more than a competitor for valuable water resources. A multi-disciplinary approach that can bring these industries together will be of Kent SeaTech Corporation IFAFS Proposal Page 23 high value. The consortium that we have assembled to address this problem will provide the multidisciplinary approach that is required. Private industry researchers from the aquaculture and agriculture fields will develop the water treatment and water sharing systems that represent the technological basis for the project. University researchers will work together with the industry scientists to design, evaluate, and refine the systems and to test the results of the water sharing programs in actual row crop field trials. The academic partners also will educate the public regarding water sharing technology through classroom activities, curriculum planning, and graduate student research activities. As the technology develops, four extension specialists (one aquaculture and one agriculture extension scientist each in CA and AZ), together with water district representatives and the Director of the USDA Western Regional Aquaculture Center, will work throughout the western states to encourage the implementation of this technology, using a combination of facility tours, publications, brochures, and audio-visual materials. All of these components are required to actually affect significant changes in irrigation water usage patterns, and can only be provided by a diverse group of cooperating specialists who are expert in the fields of system design, aquaculture and agriculture research, education, and research activities. If the project is successful and the technology is adopted by users in the western states, the USDA Regional Aquaculture Centers in other areas of the country will be in an excellent position to continue the development of water sharing concepts in other regions of the U.S. H. CONSORTIUM MANAGEMENT PLAN As the lead institution, Kent SeaTech Corporation will be responsible for coordinating the activities of the consortium members. Kent SeaTech Corporation has considerable experience in administering large federal grant programs comprised of scientists from multiple disciplines. We have served as the lead agency on several contracts that involved cooperative work by researchers from various aspects of private industry and from many academic institutions, including the University of California, University of Connecticut, University of Strasbourg, Hawaii Institute of Marine Biology, Clemson University, North Carolina State University, and San Diego State University. Based on this experience, we are aware of the need to provide means to closely coordinate the efforts of the various groups. We will monitor the progress of each consortium member by several methods, including frequent meetings, visits to consortium members, the submission of a variety of written reports (monthly database updates, monthly form-based progress reports, and quarterly and annual text reports). The monthly database reports and form-based reports will be submitted and maintained through a non-public internet website that will provide convenient means for consortium members to submit and update draft reports, databases and performance information on water treatment components, row crop yields, and other pertinent data. When sufficient information and a program for large-scale water sharing have been developed, we also will host a public website that will serve as an additional method for extension and public education activities. Kent SeaTech Corporation's Finance Department has successfully administered several million dollars of federal research funds previously, and will serve as the primary administrator of financial matters related to the consortium. We will work with each researcher's Contracts and Kent SeaTech Corporation IFAFS Proposal Page 24 Grants Office to ensure that budget requests and expenditures follow government guidelines. All requested changes in research direction, modifications in budget allocations, and proposed changes in staff commitments will be administered through our offices. We will contract for outside audits as necessary to satisfy all USDA grant requirements. Kent SeaTech Corporation also will serve as the primary interface between the consortium members and USDA IFAFS. We will serve as the focal point for the preparation of all required progress reports, annual reports, and final report. Kent SeaTech Corporation will meet with USDA Program Managers as required, develop meeting agendas and minutes, prepare digital projector presentations, arrange for travel and lodging, and provide all logistics support needed so that constructive and informative meetings will result.