BIOL 404.01
Immunology Laboratory
Spring 2008
Longwood University
Course Syllabus
Instructor:
Office:
Office hours:
Email:
Text:
Dr. David W. Buckalew
305 A Chichester Sciences Center
395-2586 (or 2586 from on-campus)
As Posted or By Appointment
buckalewdw@longwood.edu
N/A. Handouts to be distributed weekly
Objectives:
1. To perform selected experiments in the areas of immunologic serology.
2. To analyze the methods and data of the selected immunological assays.
3. To learn methodology of basic scientific research.
4. To gain proficiency in these serological diagnostic techniques used in
research, medicine, industry, and other areas of application to mankind.
Course Requirements:
There are a few general classes of requirements. These include: data sheets, a
term paper and its presentation, and exams.
1) Exams. The majority of exercises for this laboratory will be supplied
through a variety of commercial immunological laboratory kits. Given the
perishable nature of most of the necessary supplies, the exams for this part of
the course will be written but will focus on the techniques and applications of the
laboratory procedures.
2) Term Paper and Presentation: The term paper theme will be selected by
the instructor , discussed and assigned within the first few weeks of the
semester. Specific topics for each individual’s term paper need to be submitted
to the instructor for approval. Topics will be chosen on a first-come, first-served
basis. Presentation dates will be randomized for the class.
Evaluation and Grading
The tabulation of points will include:
Term paper report…(75 pts paper; 25 pts talk)..….. 100 points
Mid-term exam………………………………………… 100 points
Final exam……………………………………………… 100 points
Total points possible……………………………….……300 points
Tardiness and Absences:
Class attendance and punctuality are strongly emphasized. Please refer to
the lecture syllabus for discussion on this topic. Attendance will be taken
when required. Any student not present when roll is taken will be considered
absent. If a student arrives after roll is called, it is the responsibility of the
student to personally inform the instructor of his/her presence as soon as the
class is over. An absent or tardy student will also be held accountable for all
materials missed during absence(s).
Honor Code
I strongly support the Longwood University Honor System and expect you to do
likewise. All written work is to be pledged in full and signed. Any student found
responsible for an Honor Code violation in this laboratory will receive an “F”
grade for the entire course.
Additional Note:
The safety and housekeeping policies discussed in the lab introduction
and any similar instructions given toward that end must be adhered to
faithfully. Consumption of food or drink in the lab is expressly
forbidden.
Laboratory Schedule
Week of:
BIOL 495 Immunology
Spring 2008
Exercise
Jan
14
1. Introduction and review of term project prospectus
Jan
21
2. Blood typing using antisera
Jan
28
3. Hematocrits for leukocytes
Feb
4
4. Ultrastructure and function of 1º/2º lymph organs
Feb
11
5. Direct and Indirect ELISA
Feb
18
6. ELISA and disease detection
Feb
25
7. Mid-term Lab Examination
Mar
3
Mar
10
Mar
17
10. Ouchterlony determination of cross-reactivity
Mar
24
11. Vaccine Readiness for Biowarfare
Mar
31
12. Diagnostic Immunoblots
Apr
8
Apr
15
14. Presentation of term projects
Apr
19
15. Final Lab Examination
8. Radial Immunodiffusion
9. Spring Break Week
13. Applications of Immunofluorescence