BIOL 404.01 Immunology Laboratory Spring 2010 Longwood University Course Syllabus Instructor: Office: Office hours: Email: Text: Dr. David W. Buckalew 305 A Chichester Sciences Center 395-2586 (or 2586 from on-campus) As Posted or By Appointment buckalewdw@longwood.edu N/A. Handouts to be distributed weekly Objectives: 1. To perform selected experiments in the areas of immunologic serology. 2. To analyze the methods and data of the selected immunological assays. 3. To learn methodology of basic scientific research. 4. To gain proficiency in these serological diagnostic techniques used in research, medicine, industry, and other areas of application to mankind. Course Requirements: There are a few general classes of requirements. These include: data sheets, a term paper and its presentation, and exams. 1) Exams. The majority of exercises for this laboratory will be supplied through a variety of commercial immunological laboratory kits. Given the perishable nature of most of the necessary supplies, the exams for this part of the course will be written but will focus on the techniques and applications of the laboratory procedures. 2) Term Paper and Presentation: The term paper theme will be selected by the instructor , discussed and assigned within the first few weeks of the semester. Specific topics for each individual’s term paper need to be submitted to the instructor for approval. Topics will be chosen on a first-come, first-served basis. Presentation dates will be randomized for the class. Evaluation and Grading The tabulation of points will include: Term paper report…(75 pts paper; 50 pts talk)..…..125 points Mid-term exam………………………………………… 100 points Total points possible……………………………….……200 points Tardiness and Absences: Class attendance and punctuality are strongly emphasized. Please refer to the lecture syllabus for discussion on this topic. Attendance will be taken when required. Any student not present when roll is taken will be considered absent. If a student arrives after roll is called, it is the responsibility of the student to personally inform the instructor of his/her presence as soon as the class is over. An absent or tardy student will also be held accountable for all materials missed during absence(s). Honor Code I strongly support the Longwood University Honor System and expect you to do likewise. All written work is to be pledged in full and signed. Any student found responsible for an Honor Code violation in this laboratory will receive an “F” grade for the entire course. Additional Note: The safety and housekeeping policies discussed in the lab introduction and any similar instructions given toward that end must be adhered to faithfully. Consumption of food or drink in the lab is expressly forbidden. Laboratory Schedule Week of: BIOL 495 Immunology Spring 2010 Exercise Jan 11 1. Introduction and review of term project prospectus Jan 18 2. Blood typing using antisera Jan 25 3. Hematocrits for leukocytes Feb 1 4. Direct and Indirect ELISA Feb 8 5. Radial Immunodiffusion Feb 15 6. Ultrastructure and function of 1º/2º lymph organs Feb 22 7. Mid-term Lab Examination Mar 1 8. ELISA and disease detection Mar 8 9. Spring Break Week Mar 15 10. Ouchterlony determination of cross-reactivity Mar 22 11. Vaccine Readiness for Biowarfare Mar 29 12. Diagnostic Immunoblots Apr 5 13. Presentation of term projects Apr 12 14. Presentation of term projects Apr 19 15. Final Review